Application of RT-PCR for the detection of avian reovirus contamination in avian viral vaccines
An efficient procedure for the detection of avian reovirus (ARV)-specific RNA sequences in veterinary immunological medicinal products using reverse transcriptase polymerase chain reaction (RT-PCR) is described. Four ARV vaccine strains (1133, 1733, 2408 and Olson WVU2937), two ATCC strains (VR826 a...
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| Veröffentlicht in: | Journal of virological methods 2005-02, Vol.123 (2), p.179-186 |
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| creator | Bruhn, Silke Bruckner, Lukas Ottiger, Hans-Peter |
| description | An efficient procedure for the detection of avian reovirus (ARV)-specific RNA sequences in veterinary immunological medicinal products using reverse transcriptase polymerase chain reaction (RT-PCR) is described. Four ARV vaccine strains (1133, 1733, 2408 and Olson WVU2937), two ATCC strains (VR826 and VR856) as well as several ARV field isolates obtained from domestic, wild and pet birds could be easily detected with S2- and S4-specific primers. The identity of the amplified fragments was confirmed by restriction endonuclease analysis and sequence analysis. Furthermore, not only the starting substrates used for the production of a vaccine, but also additives and the manufacturing process (matrix effects) can influence the results. These RT-PCRs have been useful for screening poultry vaccines for extraneous ARV in the quality control of biologicals used in veterinary medicine. |
| doi_str_mv | 10.1016/j.jviromet.2004.09.019 |
| format | Article |
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Four ARV vaccine strains (1133, 1733, 2408 and Olson WVU2937), two ATCC strains (VR826 and VR856) as well as several ARV field isolates obtained from domestic, wild and pet birds could be easily detected with S2- and S4-specific primers. The identity of the amplified fragments was confirmed by restriction endonuclease analysis and sequence analysis. Furthermore, not only the starting substrates used for the production of a vaccine, but also additives and the manufacturing process (matrix effects) can influence the results. 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Four ARV vaccine strains (1133, 1733, 2408 and Olson WVU2937), two ATCC strains (VR826 and VR856) as well as several ARV field isolates obtained from domestic, wild and pet birds could be easily detected with S2- and S4-specific primers. The identity of the amplified fragments was confirmed by restriction endonuclease analysis and sequence analysis. Furthermore, not only the starting substrates used for the production of a vaccine, but also additives and the manufacturing process (matrix effects) can influence the results. These RT-PCRs have been useful for screening poultry vaccines for extraneous ARV in the quality control of biologicals used in veterinary medicine.</description><subject>Animals</subject><subject>Avian reovirus</subject><subject>Biological and medical sciences</subject><subject>Bird Diseases - virology</subject><subject>Drug Contamination</subject><subject>Extraneous agents</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Orthoreovirus, Avian - genetics</subject><subject>Orthoreovirus, Avian - isolation & purification</subject><subject>Poultry vaccines</subject><subject>Quality Control</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RT-PCR</subject><subject>Sensitivity and Specificity</subject><subject>Techniques used in virology</subject><subject>Vaccine contamination</subject><subject>Viral Vaccines - analysis</subject><subject>Viral Vaccines - standards</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdFK5DAUhsOirKO7ryC90bvWkyZpmztlcFdBUESvQ5qesBnaZDbpDPj2RqbipVcJ_N-fE85HyDmFigJtrjbVZu9imHCuagBegayAyh9kRbtWliA7fkRWGWzynfETcprSBgBEy9hPckJFUwMHWBF1s92OzujZBV8EWzy_lE_r58KGWMz_sBhwRvOZ6b3TvogY8uRdKkzws56cP3SdX_Ic6rHYa2Ocx_SLHFs9Jvy9nGfk9c_ty_qufHj8e7--eSgNr9u55J2ksheIPXAttGSS055p3dW9EIOVIKgxsusRrEEEYTtqaYsMrdUd9B07I5eHd7cx_N9hmtXkksFx1B7DLqmmZbxlIL4FaylYI1uZweYAmhhSimjVNrpJxzdFQX04UBv16UB9OFAgVXaQi-fLhF0_4fBVW5aegYsF0Mno0UbtjUtfXMOBcUozd33gMC9u7zCqZBx6g4OL2YoagvvuL-9XI6mW</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Bruhn, Silke</creator><creator>Bruckner, Lukas</creator><creator>Ottiger, Hans-Peter</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>7X8</scope></search><sort><creationdate>20050201</creationdate><title>Application of RT-PCR for the detection of avian reovirus contamination in avian viral vaccines</title><author>Bruhn, Silke ; Bruckner, Lukas ; Ottiger, Hans-Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-48919b5eeb04a5a93941b3aa82b55df9051cc98be0fcee05f81f17e3effa80b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Avian reovirus</topic><topic>Biological and medical sciences</topic><topic>Bird Diseases - virology</topic><topic>Drug Contamination</topic><topic>Extraneous agents</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Orthoreovirus, Avian - genetics</topic><topic>Orthoreovirus, Avian - isolation & purification</topic><topic>Poultry vaccines</topic><topic>Quality Control</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RT-PCR</topic><topic>Sensitivity and Specificity</topic><topic>Techniques used in virology</topic><topic>Vaccine contamination</topic><topic>Viral Vaccines - analysis</topic><topic>Viral Vaccines - standards</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bruhn, Silke</creatorcontrib><creatorcontrib>Bruckner, Lukas</creatorcontrib><creatorcontrib>Ottiger, Hans-Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bruhn, Silke</au><au>Bruckner, Lukas</au><au>Ottiger, Hans-Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of RT-PCR for the detection of avian reovirus contamination in avian viral vaccines</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>123</volume><issue>2</issue><spage>179</spage><epage>186</epage><pages>179-186</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>An efficient procedure for the detection of avian reovirus (ARV)-specific RNA sequences in veterinary immunological medicinal products using reverse transcriptase polymerase chain reaction (RT-PCR) is described. 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| ispartof | Journal of virological methods, 2005-02, Vol.123 (2), p.179-186 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Avian reovirus Biological and medical sciences Bird Diseases - virology Drug Contamination Extraneous agents Fundamental and applied biological sciences. Psychology Microbiology Orthoreovirus, Avian - genetics Orthoreovirus, Avian - isolation & purification Poultry vaccines Quality Control Reverse Transcriptase Polymerase Chain Reaction - methods RT-PCR Sensitivity and Specificity Techniques used in virology Vaccine contamination Viral Vaccines - analysis Viral Vaccines - standards Virology |
| title | Application of RT-PCR for the detection of avian reovirus contamination in avian viral vaccines |
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