Variation of T-type calcium channel protein expression affects cell division of cultured tumor cells
In this study we investigated the T-type calcium channel and its involvement in the cell division of U87MG cultured glioma cells and N1E-115 neuroblastoma cells. Using Western blot analysis, we found that expression of both α1G and α1H subunits of the T-type calcium channel decreased during conditio...
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| Veröffentlicht in: | Cell calcium (Edinburgh) 2005-02, Vol.37 (2), p.105-119 |
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| container_title | Cell calcium (Edinburgh) |
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| creator | Panner, Amith Cribbs, Leanne L. Zainelli, Gina M. Origitano, Thomas C. Singh, Sanjay Wurster, Robert D. |
| description | In this study we investigated the T-type calcium channel and its involvement in the cell division of U87MG cultured glioma cells and N1E-115 neuroblastoma cells. Using Western blot analysis, we found that expression of both α1G and α1H subunits of the T-type calcium channel decreased during conditions associated with a decrease in proliferation as evidenced by increased expression of cyclin D1, a marker for non-proliferating cells. Both serum starvation and application of mibefradil, a selective T-type calcium channel antagonist, resulted in a 50% decrease in the expression of α1G and α1H and a 700–900% increase in levels of cyclin D1 in U87MG and N1E-115 cells, respectively. Furthermore, overexpression of the α1H subunit resulted in a two-fold increase in cell proliferation compared to control cultures or cultures receiving an empty vector. In contrast, blocking expression of the α1G subunit using antisense oligonucleotides lead to a 70% decrease in proliferation of U87MG and N1E-115 cells compared to control cultures or cultures receiving a scrambled oligonucleotide. Our findings suggest that proliferation of U87MG glioma cells and N1E-115 is regulated by T-type calcium channel expression. |
| doi_str_mv | 10.1016/j.ceca.2004.07.002 |
| format | Article |
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Using Western blot analysis, we found that expression of both α1G and α1H subunits of the T-type calcium channel decreased during conditions associated with a decrease in proliferation as evidenced by increased expression of cyclin D1, a marker for non-proliferating cells. Both serum starvation and application of mibefradil, a selective T-type calcium channel antagonist, resulted in a 50% decrease in the expression of α1G and α1H and a 700–900% increase in levels of cyclin D1 in U87MG and N1E-115 cells, respectively. Furthermore, overexpression of the α1H subunit resulted in a two-fold increase in cell proliferation compared to control cultures or cultures receiving an empty vector. In contrast, blocking expression of the α1G subunit using antisense oligonucleotides lead to a 70% decrease in proliferation of U87MG and N1E-115 cells compared to control cultures or cultures receiving a scrambled oligonucleotide. Our findings suggest that proliferation of U87MG glioma cells and N1E-115 is regulated by T-type calcium channel expression.</description><identifier>ISSN: 0143-4160</identifier><identifier>EISSN: 1532-1991</identifier><identifier>DOI: 10.1016/j.ceca.2004.07.002</identifier><identifier>PMID: 15589991</identifier><language>eng</language><publisher>Netherlands: Elsevier India Pvt Ltd</publisher><subject>Animals ; Astrocytoma - metabolism ; Blotting, Western ; Calcium ; Calcium channel antagonists ; Calcium Channel Blockers - pharmacology ; Calcium Channels, T-Type - biosynthesis ; Calcium Channels, T-Type - drug effects ; Calcium Channels, T-Type - genetics ; Cell Division - physiology ; Cercopithecus aethiops ; COS Cells ; Glioma ; Humans ; Mibefradil - pharmacology ; Proliferation ; Time Factors ; Tumor Cells, Cultured ; Voltage-gated calcium channels</subject><ispartof>Cell calcium (Edinburgh), 2005-02, Vol.37 (2), p.105-119</ispartof><rights>2004 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-aef20bfca34c8f358c6a1b6d46d483347f34b6950ea50e8fa2acd10e113623d93</citedby><cites>FETCH-LOGICAL-c385t-aef20bfca34c8f358c6a1b6d46d483347f34b6950ea50e8fa2acd10e113623d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ceca.2004.07.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15589991$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Panner, Amith</creatorcontrib><creatorcontrib>Cribbs, Leanne L.</creatorcontrib><creatorcontrib>Zainelli, Gina M.</creatorcontrib><creatorcontrib>Origitano, Thomas C.</creatorcontrib><creatorcontrib>Singh, Sanjay</creatorcontrib><creatorcontrib>Wurster, Robert D.</creatorcontrib><title>Variation of T-type calcium channel protein expression affects cell division of cultured tumor cells</title><title>Cell calcium (Edinburgh)</title><addtitle>Cell Calcium</addtitle><description>In this study we investigated the T-type calcium channel and its involvement in the cell division of U87MG cultured glioma cells and N1E-115 neuroblastoma cells. Using Western blot analysis, we found that expression of both α1G and α1H subunits of the T-type calcium channel decreased during conditions associated with a decrease in proliferation as evidenced by increased expression of cyclin D1, a marker for non-proliferating cells. Both serum starvation and application of mibefradil, a selective T-type calcium channel antagonist, resulted in a 50% decrease in the expression of α1G and α1H and a 700–900% increase in levels of cyclin D1 in U87MG and N1E-115 cells, respectively. Furthermore, overexpression of the α1H subunit resulted in a two-fold increase in cell proliferation compared to control cultures or cultures receiving an empty vector. In contrast, blocking expression of the α1G subunit using antisense oligonucleotides lead to a 70% decrease in proliferation of U87MG and N1E-115 cells compared to control cultures or cultures receiving a scrambled oligonucleotide. Our findings suggest that proliferation of U87MG glioma cells and N1E-115 is regulated by T-type calcium channel expression.</description><subject>Animals</subject><subject>Astrocytoma - metabolism</subject><subject>Blotting, Western</subject><subject>Calcium</subject><subject>Calcium channel antagonists</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Calcium Channels, T-Type - biosynthesis</subject><subject>Calcium Channels, T-Type - drug effects</subject><subject>Calcium Channels, T-Type - genetics</subject><subject>Cell Division - physiology</subject><subject>Cercopithecus aethiops</subject><subject>COS Cells</subject><subject>Glioma</subject><subject>Humans</subject><subject>Mibefradil - pharmacology</subject><subject>Proliferation</subject><subject>Time Factors</subject><subject>Tumor Cells, Cultured</subject><subject>Voltage-gated calcium channels</subject><issn>0143-4160</issn><issn>1532-1991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rGzEQhkVIiR23fyCHoFNvuxl97Bf0UkLaBgy5uL0KrTQiMvvhSrum_vfVxobeGhgxBz3zzvC-hNwxyBmw8mGfGzQ65wAyhyoH4FdkzQrBM9Y07JqsgUmRSVbCitzGuAeARlTshqxYUdRNYtbE_tLB68mPAx0d3WXT6YDU6M74uafmVQ8DdvQQxgn9QPHPIWCMC6ydQzNFarDrqPVHHy8SZu6mOaCl09yP4e0_fiQfnO4ifrr0Dfn57Wn3-CPbvnx_fvy6zYyoiynT6Di0zmghTe1EUZtSs7a0MlUthKyckG3ZFIA6vdppro1lgIyJkgvbiA35fNZNB_-eMU6q93G5QA84zlGVlZCylPJdkFUV5zIt3RB-Bk0YYwzo1CH4XoeTYqCWENReLSGoJQQFlUohpKH7i_rc9mj_jVxcT8CXM4DJjKPHoKLxOBi0PiRXlR39__T_AoOWmYI</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Panner, Amith</creator><creator>Cribbs, Leanne L.</creator><creator>Zainelli, Gina M.</creator><creator>Origitano, Thomas C.</creator><creator>Singh, Sanjay</creator><creator>Wurster, Robert D.</creator><general>Elsevier India Pvt Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>20050201</creationdate><title>Variation of T-type calcium channel protein expression affects cell division of cultured tumor cells</title><author>Panner, Amith ; Cribbs, Leanne L. ; Zainelli, Gina M. ; Origitano, Thomas C. ; Singh, Sanjay ; Wurster, Robert D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-aef20bfca34c8f358c6a1b6d46d483347f34b6950ea50e8fa2acd10e113623d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Astrocytoma - metabolism</topic><topic>Blotting, Western</topic><topic>Calcium</topic><topic>Calcium channel antagonists</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Calcium Channels, T-Type - biosynthesis</topic><topic>Calcium Channels, T-Type - drug effects</topic><topic>Calcium Channels, T-Type - genetics</topic><topic>Cell Division - physiology</topic><topic>Cercopithecus aethiops</topic><topic>COS Cells</topic><topic>Glioma</topic><topic>Humans</topic><topic>Mibefradil - pharmacology</topic><topic>Proliferation</topic><topic>Time Factors</topic><topic>Tumor Cells, Cultured</topic><topic>Voltage-gated calcium channels</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Panner, Amith</creatorcontrib><creatorcontrib>Cribbs, Leanne L.</creatorcontrib><creatorcontrib>Zainelli, Gina M.</creatorcontrib><creatorcontrib>Origitano, Thomas C.</creatorcontrib><creatorcontrib>Singh, Sanjay</creatorcontrib><creatorcontrib>Wurster, Robert D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell calcium (Edinburgh)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Panner, Amith</au><au>Cribbs, Leanne L.</au><au>Zainelli, Gina M.</au><au>Origitano, Thomas C.</au><au>Singh, Sanjay</au><au>Wurster, Robert D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variation of T-type calcium channel protein expression affects cell division of cultured tumor cells</atitle><jtitle>Cell calcium (Edinburgh)</jtitle><addtitle>Cell Calcium</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>37</volume><issue>2</issue><spage>105</spage><epage>119</epage><pages>105-119</pages><issn>0143-4160</issn><eissn>1532-1991</eissn><abstract>In this study we investigated the T-type calcium channel and its involvement in the cell division of U87MG cultured glioma cells and N1E-115 neuroblastoma cells. Using Western blot analysis, we found that expression of both α1G and α1H subunits of the T-type calcium channel decreased during conditions associated with a decrease in proliferation as evidenced by increased expression of cyclin D1, a marker for non-proliferating cells. Both serum starvation and application of mibefradil, a selective T-type calcium channel antagonist, resulted in a 50% decrease in the expression of α1G and α1H and a 700–900% increase in levels of cyclin D1 in U87MG and N1E-115 cells, respectively. Furthermore, overexpression of the α1H subunit resulted in a two-fold increase in cell proliferation compared to control cultures or cultures receiving an empty vector. In contrast, blocking expression of the α1G subunit using antisense oligonucleotides lead to a 70% decrease in proliferation of U87MG and N1E-115 cells compared to control cultures or cultures receiving a scrambled oligonucleotide. 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| subjects | Animals Astrocytoma - metabolism Blotting, Western Calcium Calcium channel antagonists Calcium Channel Blockers - pharmacology Calcium Channels, T-Type - biosynthesis Calcium Channels, T-Type - drug effects Calcium Channels, T-Type - genetics Cell Division - physiology Cercopithecus aethiops COS Cells Glioma Humans Mibefradil - pharmacology Proliferation Time Factors Tumor Cells, Cultured Voltage-gated calcium channels |
| title | Variation of T-type calcium channel protein expression affects cell division of cultured tumor cells |
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