Use of Vaginal Polymorphonuclear to Epithelial Cell Ratios for the Prediction of Preterm Birth
OBJECTIVE:To compare the use of midtrimester vaginal polymorphonuclear cell (PMN) counts on Gram-stain preparations with PMN to epithelial cell (EPI) ratios for the prediction of subsequent spontaneous preterm birth. METHODS:From a prospective cohort study, 83 women with a spontaneous preterm birth...
Gespeichert in:
| Veröffentlicht in: | Obstetrics and gynecology (New York. 1953) 2005-01, Vol.105 (1), p.139-144 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 144 |
|---|---|
| container_issue | 1 |
| container_start_page | 139 |
| container_title | Obstetrics and gynecology (New York. 1953) |
| container_volume | 105 |
| creator | Ramsey, Patrick S. Lyon, M Dee Goepfert, Alice R. Cliver, Suzanne Schwebke, Jane Andrews, William W. Goldenberg, Robert L. Hauth, John C. |
| description | OBJECTIVE:To compare the use of midtrimester vaginal polymorphonuclear cell (PMN) counts on Gram-stain preparations with PMN to epithelial cell (EPI) ratios for the prediction of subsequent spontaneous preterm birth.
METHODS:From a prospective cohort study, 83 women with a spontaneous preterm birth at less than 35 weeks of gestation (cases) were compared with a control group of 108 women who delivered at term. Vaginal Gram-stain preparations were obtained at the initial study visit (20–25 weeks). Vaginal PMN and epithelial EPI cell counts were assessed on 5 nonadjacent oil immersion fields under × 1,000 magnification. Vaginal PMN/EPI ratios for each evaluated slide field were calculated to control for intraslide variation in cellular density.
RESULTS:Mean delivery gestational age was 31.5 ± 3.5 weeks for the cases and 39.8 ± 1.2 weeks for the controls. Mean PMN counts were similar in the cases (13 ± 20 cells per oil immersion field) and the controls (10 ± 14 cells per oil immersion field) (P = .17). The mean PMN/EPI ratio, however, was significantly higher among the cases (3.4 ± 6.0) than among the controls (1.8 ± 2.4, P = .01). When PMN counts and PMN/EPI ratios were dichotomized by the 95th percentile cutoff (based on control values), the PMN/EPI ratio was significantly associated with subsequent development of spontaneous preterm birth at less than 35 weeks of gestation (odds ratio 3.8, 95% confidence interval 1.3–11.2), whereas PMN counts were not (odds ratio 2.1, 95% confidence interval 0.7–6.1).
CONCLUSION:Midtrimester vaginal PMN counts on Gram-stain preparations were not significantly associated with subsequent spontaneous preterm birth in this population. However, the ratio of PMN to EPI counts, which provides internal standardization of variation in slide cellular density, was significantly higher in women with subsequent spontaneous preterm birth at less than 35 weeks.
LEVEL OF EVIDENCE:II-2 |
| doi_str_mv | 10.1097/01.AOG.0000148269.36622.0a |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67343227</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67343227</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4584-df5c229d1aa878e2f3555c7939aa87f84e4e03c976c08afe01f2f5cd691073e23</originalsourceid><addsrcrecordid>eNpFkG9rFDEQxoNY7Fn9CrII-m63-Z-N7-pRq1BoESt91RCzE3c1uzmTXUq_vdnewQWGMDO_Z2Z4EHpPcEOwVueYNBc3Vw0uj_CWSt0wKSltsH2BNqRVrKaM3b9EG4yprlXL-Sl6nfOflZeavUKnREgqiBAb9HCXoYq--ml_D5MN1W0MT2NMuz5OiwtgUzXH6nI3zD2EofS3EEL13c5DzJWPpdtDdZugG1wpTeukks2QxurzkOb-DTrxNmR4e_jP0N2Xyx_br_X1zdW37cV17bhoed154SjVHbG2VS1Qz4QQTmmm14JvOXDAzGklHW6tB0w8LZJOaoIVA8rO0Mf93F2K_xbIsxmH7MqtdoK4ZCMV44xSVcBPe9ClmHMCb3ZpGG16MgSb1V2DiSnumqO75tldg20RvztsWX6N0B2lBzsL8OEA2Oxs8MlObshHTnIpBVnP5XvuMYZiVv4blkdIpgcb5v55dRmIa4qxwKRkdQnK2X8K-ZLT</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67343227</pqid></control><display><type>article</type><title>Use of Vaginal Polymorphonuclear to Epithelial Cell Ratios for the Prediction of Preterm Birth</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><creator>Ramsey, Patrick S. ; Lyon, M Dee ; Goepfert, Alice R. ; Cliver, Suzanne ; Schwebke, Jane ; Andrews, William W. ; Goldenberg, Robert L. ; Hauth, John C.</creator><creatorcontrib>Ramsey, Patrick S. ; Lyon, M Dee ; Goepfert, Alice R. ; Cliver, Suzanne ; Schwebke, Jane ; Andrews, William W. ; Goldenberg, Robert L. ; Hauth, John C.</creatorcontrib><description>OBJECTIVE:To compare the use of midtrimester vaginal polymorphonuclear cell (PMN) counts on Gram-stain preparations with PMN to epithelial cell (EPI) ratios for the prediction of subsequent spontaneous preterm birth.
METHODS:From a prospective cohort study, 83 women with a spontaneous preterm birth at less than 35 weeks of gestation (cases) were compared with a control group of 108 women who delivered at term. Vaginal Gram-stain preparations were obtained at the initial study visit (20–25 weeks). Vaginal PMN and epithelial EPI cell counts were assessed on 5 nonadjacent oil immersion fields under × 1,000 magnification. Vaginal PMN/EPI ratios for each evaluated slide field were calculated to control for intraslide variation in cellular density.
RESULTS:Mean delivery gestational age was 31.5 ± 3.5 weeks for the cases and 39.8 ± 1.2 weeks for the controls. Mean PMN counts were similar in the cases (13 ± 20 cells per oil immersion field) and the controls (10 ± 14 cells per oil immersion field) (P = .17). The mean PMN/EPI ratio, however, was significantly higher among the cases (3.4 ± 6.0) than among the controls (1.8 ± 2.4, P = .01). When PMN counts and PMN/EPI ratios were dichotomized by the 95th percentile cutoff (based on control values), the PMN/EPI ratio was significantly associated with subsequent development of spontaneous preterm birth at less than 35 weeks of gestation (odds ratio 3.8, 95% confidence interval 1.3–11.2), whereas PMN counts were not (odds ratio 2.1, 95% confidence interval 0.7–6.1).
CONCLUSION:Midtrimester vaginal PMN counts on Gram-stain preparations were not significantly associated with subsequent spontaneous preterm birth in this population. However, the ratio of PMN to EPI counts, which provides internal standardization of variation in slide cellular density, was significantly higher in women with subsequent spontaneous preterm birth at less than 35 weeks.
LEVEL OF EVIDENCE:II-2</description><identifier>ISSN: 0029-7844</identifier><identifier>EISSN: 1873-233X</identifier><identifier>DOI: 10.1097/01.AOG.0000148269.36622.0a</identifier><identifier>PMID: 15625155</identifier><identifier>CODEN: OBGNAS</identifier><language>eng</language><publisher>New York, NY: The American College of Obstetricians and Gynecologists</publisher><subject>Adult ; Biological and medical sciences ; Cell Count ; Epithelial Cells - pathology ; Female ; Gynecology. Andrology. Obstetrics ; Humans ; Medical sciences ; Neutrophils - pathology ; Obstetric Labor, Premature - diagnosis ; Obstetric Labor, Premature - microbiology ; Obstetric Labor, Premature - pathology ; Pregnancy ; Pregnancy Complications, Infectious - diagnosis ; Pregnancy Trimester, Second ; Staining and Labeling ; Vagina - pathology ; Vaginosis, Bacterial - complications ; Vaginosis, Bacterial - diagnosis</subject><ispartof>Obstetrics and gynecology (New York. 1953), 2005-01, Vol.105 (1), p.139-144</ispartof><rights>2005 The American College of Obstetricians and Gynecologists</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4584-df5c229d1aa878e2f3555c7939aa87f84e4e03c976c08afe01f2f5cd691073e23</citedby><cites>FETCH-LOGICAL-c4584-df5c229d1aa878e2f3555c7939aa87f84e4e03c976c08afe01f2f5cd691073e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4023,27922,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16466512$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15625155$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramsey, Patrick S.</creatorcontrib><creatorcontrib>Lyon, M Dee</creatorcontrib><creatorcontrib>Goepfert, Alice R.</creatorcontrib><creatorcontrib>Cliver, Suzanne</creatorcontrib><creatorcontrib>Schwebke, Jane</creatorcontrib><creatorcontrib>Andrews, William W.</creatorcontrib><creatorcontrib>Goldenberg, Robert L.</creatorcontrib><creatorcontrib>Hauth, John C.</creatorcontrib><title>Use of Vaginal Polymorphonuclear to Epithelial Cell Ratios for the Prediction of Preterm Birth</title><title>Obstetrics and gynecology (New York. 1953)</title><addtitle>Obstet Gynecol</addtitle><description>OBJECTIVE:To compare the use of midtrimester vaginal polymorphonuclear cell (PMN) counts on Gram-stain preparations with PMN to epithelial cell (EPI) ratios for the prediction of subsequent spontaneous preterm birth.
METHODS:From a prospective cohort study, 83 women with a spontaneous preterm birth at less than 35 weeks of gestation (cases) were compared with a control group of 108 women who delivered at term. Vaginal Gram-stain preparations were obtained at the initial study visit (20–25 weeks). Vaginal PMN and epithelial EPI cell counts were assessed on 5 nonadjacent oil immersion fields under × 1,000 magnification. Vaginal PMN/EPI ratios for each evaluated slide field were calculated to control for intraslide variation in cellular density.
RESULTS:Mean delivery gestational age was 31.5 ± 3.5 weeks for the cases and 39.8 ± 1.2 weeks for the controls. Mean PMN counts were similar in the cases (13 ± 20 cells per oil immersion field) and the controls (10 ± 14 cells per oil immersion field) (P = .17). The mean PMN/EPI ratio, however, was significantly higher among the cases (3.4 ± 6.0) than among the controls (1.8 ± 2.4, P = .01). When PMN counts and PMN/EPI ratios were dichotomized by the 95th percentile cutoff (based on control values), the PMN/EPI ratio was significantly associated with subsequent development of spontaneous preterm birth at less than 35 weeks of gestation (odds ratio 3.8, 95% confidence interval 1.3–11.2), whereas PMN counts were not (odds ratio 2.1, 95% confidence interval 0.7–6.1).
CONCLUSION:Midtrimester vaginal PMN counts on Gram-stain preparations were not significantly associated with subsequent spontaneous preterm birth in this population. However, the ratio of PMN to EPI counts, which provides internal standardization of variation in slide cellular density, was significantly higher in women with subsequent spontaneous preterm birth at less than 35 weeks.
LEVEL OF EVIDENCE:II-2</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Epithelial Cells - pathology</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Neutrophils - pathology</subject><subject>Obstetric Labor, Premature - diagnosis</subject><subject>Obstetric Labor, Premature - microbiology</subject><subject>Obstetric Labor, Premature - pathology</subject><subject>Pregnancy</subject><subject>Pregnancy Complications, Infectious - diagnosis</subject><subject>Pregnancy Trimester, Second</subject><subject>Staining and Labeling</subject><subject>Vagina - pathology</subject><subject>Vaginosis, Bacterial - complications</subject><subject>Vaginosis, Bacterial - diagnosis</subject><issn>0029-7844</issn><issn>1873-233X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkG9rFDEQxoNY7Fn9CrII-m63-Z-N7-pRq1BoESt91RCzE3c1uzmTXUq_vdnewQWGMDO_Z2Z4EHpPcEOwVueYNBc3Vw0uj_CWSt0wKSltsH2BNqRVrKaM3b9EG4yprlXL-Sl6nfOflZeavUKnREgqiBAb9HCXoYq--ml_D5MN1W0MT2NMuz5OiwtgUzXH6nI3zD2EofS3EEL13c5DzJWPpdtDdZugG1wpTeukks2QxurzkOb-DTrxNmR4e_jP0N2Xyx_br_X1zdW37cV17bhoed154SjVHbG2VS1Qz4QQTmmm14JvOXDAzGklHW6tB0w8LZJOaoIVA8rO0Mf93F2K_xbIsxmH7MqtdoK4ZCMV44xSVcBPe9ClmHMCb3ZpGG16MgSb1V2DiSnumqO75tldg20RvztsWX6N0B2lBzsL8OEA2Oxs8MlObshHTnIpBVnP5XvuMYZiVv4blkdIpgcb5v55dRmIa4qxwKRkdQnK2X8K-ZLT</recordid><startdate>200501</startdate><enddate>200501</enddate><creator>Ramsey, Patrick S.</creator><creator>Lyon, M Dee</creator><creator>Goepfert, Alice R.</creator><creator>Cliver, Suzanne</creator><creator>Schwebke, Jane</creator><creator>Andrews, William W.</creator><creator>Goldenberg, Robert L.</creator><creator>Hauth, John C.</creator><general>The American College of Obstetricians and Gynecologists</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200501</creationdate><title>Use of Vaginal Polymorphonuclear to Epithelial Cell Ratios for the Prediction of Preterm Birth</title><author>Ramsey, Patrick S. ; Lyon, M Dee ; Goepfert, Alice R. ; Cliver, Suzanne ; Schwebke, Jane ; Andrews, William W. ; Goldenberg, Robert L. ; Hauth, John C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4584-df5c229d1aa878e2f3555c7939aa87f84e4e03c976c08afe01f2f5cd691073e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Epithelial Cells - pathology</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Neutrophils - pathology</topic><topic>Obstetric Labor, Premature - diagnosis</topic><topic>Obstetric Labor, Premature - microbiology</topic><topic>Obstetric Labor, Premature - pathology</topic><topic>Pregnancy</topic><topic>Pregnancy Complications, Infectious - diagnosis</topic><topic>Pregnancy Trimester, Second</topic><topic>Staining and Labeling</topic><topic>Vagina - pathology</topic><topic>Vaginosis, Bacterial - complications</topic><topic>Vaginosis, Bacterial - diagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramsey, Patrick S.</creatorcontrib><creatorcontrib>Lyon, M Dee</creatorcontrib><creatorcontrib>Goepfert, Alice R.</creatorcontrib><creatorcontrib>Cliver, Suzanne</creatorcontrib><creatorcontrib>Schwebke, Jane</creatorcontrib><creatorcontrib>Andrews, William W.</creatorcontrib><creatorcontrib>Goldenberg, Robert L.</creatorcontrib><creatorcontrib>Hauth, John C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Obstetrics and gynecology (New York. 1953)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramsey, Patrick S.</au><au>Lyon, M Dee</au><au>Goepfert, Alice R.</au><au>Cliver, Suzanne</au><au>Schwebke, Jane</au><au>Andrews, William W.</au><au>Goldenberg, Robert L.</au><au>Hauth, John C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of Vaginal Polymorphonuclear to Epithelial Cell Ratios for the Prediction of Preterm Birth</atitle><jtitle>Obstetrics and gynecology (New York. 1953)</jtitle><addtitle>Obstet Gynecol</addtitle><date>2005-01</date><risdate>2005</risdate><volume>105</volume><issue>1</issue><spage>139</spage><epage>144</epage><pages>139-144</pages><issn>0029-7844</issn><eissn>1873-233X</eissn><coden>OBGNAS</coden><abstract>OBJECTIVE:To compare the use of midtrimester vaginal polymorphonuclear cell (PMN) counts on Gram-stain preparations with PMN to epithelial cell (EPI) ratios for the prediction of subsequent spontaneous preterm birth.
METHODS:From a prospective cohort study, 83 women with a spontaneous preterm birth at less than 35 weeks of gestation (cases) were compared with a control group of 108 women who delivered at term. Vaginal Gram-stain preparations were obtained at the initial study visit (20–25 weeks). Vaginal PMN and epithelial EPI cell counts were assessed on 5 nonadjacent oil immersion fields under × 1,000 magnification. Vaginal PMN/EPI ratios for each evaluated slide field were calculated to control for intraslide variation in cellular density.
RESULTS:Mean delivery gestational age was 31.5 ± 3.5 weeks for the cases and 39.8 ± 1.2 weeks for the controls. Mean PMN counts were similar in the cases (13 ± 20 cells per oil immersion field) and the controls (10 ± 14 cells per oil immersion field) (P = .17). The mean PMN/EPI ratio, however, was significantly higher among the cases (3.4 ± 6.0) than among the controls (1.8 ± 2.4, P = .01). When PMN counts and PMN/EPI ratios were dichotomized by the 95th percentile cutoff (based on control values), the PMN/EPI ratio was significantly associated with subsequent development of spontaneous preterm birth at less than 35 weeks of gestation (odds ratio 3.8, 95% confidence interval 1.3–11.2), whereas PMN counts were not (odds ratio 2.1, 95% confidence interval 0.7–6.1).
CONCLUSION:Midtrimester vaginal PMN counts on Gram-stain preparations were not significantly associated with subsequent spontaneous preterm birth in this population. However, the ratio of PMN to EPI counts, which provides internal standardization of variation in slide cellular density, was significantly higher in women with subsequent spontaneous preterm birth at less than 35 weeks.
LEVEL OF EVIDENCE:II-2</abstract><cop>New York, NY</cop><pub>The American College of Obstetricians and Gynecologists</pub><pmid>15625155</pmid><doi>10.1097/01.AOG.0000148269.36622.0a</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0029-7844 |
| ispartof | Obstetrics and gynecology (New York. 1953), 2005-01, Vol.105 (1), p.139-144 |
| issn | 0029-7844 1873-233X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67343227 |
| source | MEDLINE; Journals@Ovid Complete |
| subjects | Adult Biological and medical sciences Cell Count Epithelial Cells - pathology Female Gynecology. Andrology. Obstetrics Humans Medical sciences Neutrophils - pathology Obstetric Labor, Premature - diagnosis Obstetric Labor, Premature - microbiology Obstetric Labor, Premature - pathology Pregnancy Pregnancy Complications, Infectious - diagnosis Pregnancy Trimester, Second Staining and Labeling Vagina - pathology Vaginosis, Bacterial - complications Vaginosis, Bacterial - diagnosis |
| title | Use of Vaginal Polymorphonuclear to Epithelial Cell Ratios for the Prediction of Preterm Birth |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T13%3A42%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Use%20of%20Vaginal%20Polymorphonuclear%20to%20Epithelial%20Cell%20Ratios%20for%20the%20Prediction%20of%20Preterm%20Birth&rft.jtitle=Obstetrics%20and%20gynecology%20(New%20York.%201953)&rft.au=Ramsey,%20Patrick%20S.&rft.date=2005-01&rft.volume=105&rft.issue=1&rft.spage=139&rft.epage=144&rft.pages=139-144&rft.issn=0029-7844&rft.eissn=1873-233X&rft.coden=OBGNAS&rft_id=info:doi/10.1097/01.AOG.0000148269.36622.0a&rft_dat=%3Cproquest_cross%3E67343227%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67343227&rft_id=info:pmid/15625155&rfr_iscdi=true |