Myocardial contraction is 5-fold more economical in ventricular than in atrial human tissue
Cardiac energetics and performance depend on the expression level of the fast (alpha-) and slow (beta-) myosin heavy chain (MHC) isoform. In ventricular tissue, the beta-MHC isoform predominates, whereas in atrial tissue a variable mixture of alpha- and beta-MHC is found. In several cardiac diseases...
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| Veröffentlicht in: | Cardiovascular research 2005, Vol.65 (1), p.221-229 |
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| creator | NAROLSKA, N. A VAN LOON, R. B VAN DER VELDEN, J STIENEN, G. J. M BOONTJE, N. M ZAREMBA, R EIRAS PENAS, S RUSSELL, J SPIEGELENBERG, S. R HUYBREGTS, M. A. J. M VISSER, F. C DE JONG, J. W |
| description | Cardiac energetics and performance depend on the expression level of the fast (alpha-) and slow (beta-) myosin heavy chain (MHC) isoform. In ventricular tissue, the beta-MHC isoform predominates, whereas in atrial tissue a variable mixture of alpha- and beta-MHC is found. In several cardiac diseases, the slow isoform is upregulated; however, the functional implications of this transition in human myocardium are largely unknown. The aim of this study was to determine the relation between contractile properties and MHC isoform composition in healthy human myocardium using the diversity in atrial tissue.
Isometric force production and ATP consumption were measured in chemically skinned atrial trabeculae and ventricular muscle strips, and rate of force redevelopment was studied using single cardiomyocytes. MHC isoform composition was determined by one-dimensional SDS-gel electrophoresis.
Force development in ventricular tissue was about 5-fold more economical, but nine times slower, than in atrial tissue. Significant linear correlations were found between MHC isoform composition, ATP consumption and rate of force redevelopment.
These results clearly indicate that even a minor shift in MHC isoform expression has considerable impact on cardiac performance in human tissue. |
| doi_str_mv | 10.1016/j.cardiores.2004.09.029 |
| format | Article |
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Isometric force production and ATP consumption were measured in chemically skinned atrial trabeculae and ventricular muscle strips, and rate of force redevelopment was studied using single cardiomyocytes. MHC isoform composition was determined by one-dimensional SDS-gel electrophoresis.
Force development in ventricular tissue was about 5-fold more economical, but nine times slower, than in atrial tissue. Significant linear correlations were found between MHC isoform composition, ATP consumption and rate of force redevelopment.
These results clearly indicate that even a minor shift in MHC isoform expression has considerable impact on cardiac performance in human tissue.</description><identifier>ISSN: 0008-6363</identifier><identifier>EISSN: 1755-3245</identifier><identifier>DOI: 10.1016/j.cardiores.2004.09.029</identifier><identifier>PMID: 15621050</identifier><identifier>CODEN: CVREAU</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adenosine Triphosphate - metabolism ; Adult ; Aged ; Aged, 80 and over ; Atrial Function - physiology ; Biological and medical sciences ; Biomechanical Phenomena ; Cardiology. Vascular system ; Electrophoresis, Polyacrylamide Gel ; Heart ; Heart Atria ; Heart failure, cardiogenic pulmonary edema, cardiac enlargement ; Heart Ventricles ; Humans ; Medical sciences ; Middle Aged ; Myocardial Contraction - physiology ; Myocardium - metabolism ; Myosin Heavy Chains - metabolism ; Protein Isoforms - metabolism ; Ventricular Function - physiology</subject><ispartof>Cardiovascular research, 2005, Vol.65 (1), p.221-229</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-3923f0057afa20eaf583b76fefbb5fc56ef47d31437ea714c6a113f37ae952243</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16399628$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15621050$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NAROLSKA, N. A</creatorcontrib><creatorcontrib>VAN LOON, R. B</creatorcontrib><creatorcontrib>VAN DER VELDEN, J</creatorcontrib><creatorcontrib>STIENEN, G. J. M</creatorcontrib><creatorcontrib>BOONTJE, N. M</creatorcontrib><creatorcontrib>ZAREMBA, R</creatorcontrib><creatorcontrib>EIRAS PENAS, S</creatorcontrib><creatorcontrib>RUSSELL, J</creatorcontrib><creatorcontrib>SPIEGELENBERG, S. R</creatorcontrib><creatorcontrib>HUYBREGTS, M. A. J. M</creatorcontrib><creatorcontrib>VISSER, F. C</creatorcontrib><creatorcontrib>DE JONG, J. W</creatorcontrib><title>Myocardial contraction is 5-fold more economical in ventricular than in atrial human tissue</title><title>Cardiovascular research</title><addtitle>Cardiovasc Res</addtitle><description>Cardiac energetics and performance depend on the expression level of the fast (alpha-) and slow (beta-) myosin heavy chain (MHC) isoform. In ventricular tissue, the beta-MHC isoform predominates, whereas in atrial tissue a variable mixture of alpha- and beta-MHC is found. In several cardiac diseases, the slow isoform is upregulated; however, the functional implications of this transition in human myocardium are largely unknown. The aim of this study was to determine the relation between contractile properties and MHC isoform composition in healthy human myocardium using the diversity in atrial tissue.
Isometric force production and ATP consumption were measured in chemically skinned atrial trabeculae and ventricular muscle strips, and rate of force redevelopment was studied using single cardiomyocytes. MHC isoform composition was determined by one-dimensional SDS-gel electrophoresis.
Force development in ventricular tissue was about 5-fold more economical, but nine times slower, than in atrial tissue. Significant linear correlations were found between MHC isoform composition, ATP consumption and rate of force redevelopment.
These results clearly indicate that even a minor shift in MHC isoform expression has considerable impact on cardiac performance in human tissue.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Atrial Function - physiology</subject><subject>Biological and medical sciences</subject><subject>Biomechanical Phenomena</subject><subject>Cardiology. Vascular system</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Heart</subject><subject>Heart Atria</subject><subject>Heart failure, cardiogenic pulmonary edema, cardiac enlargement</subject><subject>Heart Ventricles</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Myocardial Contraction - physiology</subject><subject>Myocardium - metabolism</subject><subject>Myosin Heavy Chains - metabolism</subject><subject>Protein Isoforms - metabolism</subject><subject>Ventricular Function - physiology</subject><issn>0008-6363</issn><issn>1755-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMlOwzAQQC0EoqXwC5AL3BK8xHZzRBWbVMQFThysiWOrrpK42AlS_x6XVvQ0mpk3ix5CNwQXBBNxvy40hMb5YGJBMS4LXBWYVidoSiTnOaMlP0VTjPE8F0ywCbqIcZ1SzmV5jiaEC0owx1P09bb1f6ugzbTvhwB6cL7PXMx4bn3bZF06kpnU853TiXJ99mMS6PTYQsiGFfS7GqRK6q7GLuWDi3E0l-jMQhvN1SHO0OfT48fiJV--P78uHpa5ZhUeclZRZtNrEixQbMDyOaulsMbWNbeaC2NL2TBSMmlAklILIIRZJsFUnNKSzdDdfu8m-O_RxEF1LmrTttAbP0YlJCupFDSBcg_q4GMMxqpNcB2ErSJY7byqtfr3qnZeFa5U8pomrw8nxrozzXHuIDIBtwcAYrJkA_TaxSMnWFUJOme_0aGFGw</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>NAROLSKA, N. 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Vascular system</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Heart</topic><topic>Heart Atria</topic><topic>Heart failure, cardiogenic pulmonary edema, cardiac enlargement</topic><topic>Heart Ventricles</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Myocardial Contraction - physiology</topic><topic>Myocardium - metabolism</topic><topic>Myosin Heavy Chains - metabolism</topic><topic>Protein Isoforms - metabolism</topic><topic>Ventricular Function - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NAROLSKA, N. A</creatorcontrib><creatorcontrib>VAN LOON, R. B</creatorcontrib><creatorcontrib>VAN DER VELDEN, J</creatorcontrib><creatorcontrib>STIENEN, G. J. M</creatorcontrib><creatorcontrib>BOONTJE, N. 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M</au><au>ZAREMBA, R</au><au>EIRAS PENAS, S</au><au>RUSSELL, J</au><au>SPIEGELENBERG, S. R</au><au>HUYBREGTS, M. A. J. M</au><au>VISSER, F. C</au><au>DE JONG, J. W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Myocardial contraction is 5-fold more economical in ventricular than in atrial human tissue</atitle><jtitle>Cardiovascular research</jtitle><addtitle>Cardiovasc Res</addtitle><date>2005</date><risdate>2005</risdate><volume>65</volume><issue>1</issue><spage>221</spage><epage>229</epage><pages>221-229</pages><issn>0008-6363</issn><eissn>1755-3245</eissn><coden>CVREAU</coden><abstract>Cardiac energetics and performance depend on the expression level of the fast (alpha-) and slow (beta-) myosin heavy chain (MHC) isoform. In ventricular tissue, the beta-MHC isoform predominates, whereas in atrial tissue a variable mixture of alpha- and beta-MHC is found. In several cardiac diseases, the slow isoform is upregulated; however, the functional implications of this transition in human myocardium are largely unknown. The aim of this study was to determine the relation between contractile properties and MHC isoform composition in healthy human myocardium using the diversity in atrial tissue.
Isometric force production and ATP consumption were measured in chemically skinned atrial trabeculae and ventricular muscle strips, and rate of force redevelopment was studied using single cardiomyocytes. MHC isoform composition was determined by one-dimensional SDS-gel electrophoresis.
Force development in ventricular tissue was about 5-fold more economical, but nine times slower, than in atrial tissue. Significant linear correlations were found between MHC isoform composition, ATP consumption and rate of force redevelopment.
These results clearly indicate that even a minor shift in MHC isoform expression has considerable impact on cardiac performance in human tissue.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>15621050</pmid><doi>10.1016/j.cardiores.2004.09.029</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adenosine Triphosphate - metabolism Adult Aged Aged, 80 and over Atrial Function - physiology Biological and medical sciences Biomechanical Phenomena Cardiology. Vascular system Electrophoresis, Polyacrylamide Gel Heart Heart Atria Heart failure, cardiogenic pulmonary edema, cardiac enlargement Heart Ventricles Humans Medical sciences Middle Aged Myocardial Contraction - physiology Myocardium - metabolism Myosin Heavy Chains - metabolism Protein Isoforms - metabolism Ventricular Function - physiology |
| title | Myocardial contraction is 5-fold more economical in ventricular than in atrial human tissue |
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