Effects of fluorescent dyes, quenchers, and dangling ends on DNA duplex stability

Single and dual-labeled fluorescent oligodeoxynucleotides are used in many molecular biology applications. We investigated the effects of commonly used fluorescent dyes and quenchers on the thermodynamic stability of a model probe–target DNA duplex. We demonstrate that those effects can be significa...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 2005-02, Vol.327 (2), p.473-484
Hauptverfasser:	Moreira, Bernardo G., You, Yong, Behlke, Mark A., Owczarzy, Richard
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Dangling ends DNA - chemistry DNA - drug effects DNA - genetics DNA - metabolism DNA melting temperature DNA thermodynamics Fluorescent Dyes - chemistry Fluorescent Dyes - pharmacology Fluorescent probes Nucleic Acid Denaturation - drug effects Quencher Real-time PCR Spectrum Analysis Temperature Thermodynamics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!