Simultaneous determination of anions in nanoliter volumes
Simultaneous determination of anions in nanoliter volumes. The study of renal tubular transport requires the ability to accurately measure ion concentrations in samples taken from single tubules. Sample collection and analysis are laborious, so methods allowing determination of multiple ion species...
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| Veröffentlicht in: | Kidney international 2005-01, Vol.67 (1), p.357-363 |
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| creator | Faria, Nuno J.R. Dobbie, Hamish Slater, Jonathan M. Shirley, David G. Stocking, Christopher J. Unwin, Robert J. |
| description | Simultaneous determination of anions in nanoliter volumes.
The study of renal tubular transport requires the ability to accurately measure ion concentrations in samples taken from single tubules. Sample collection and analysis are laborious, so methods allowing determination of multiple ion species in a small volume sample are advantageous. This article describes a method for the simultaneous analysis of anions at physiologic concentrations in nanoliter volumes of tubular fluid.
The analysis is performed using capillary zone electrophoresis. Diluted samples are moved along a capillary by bulk transport and separated according to charge and size. Peaks corresponding to anions are obtained by ultraviolet (UV) detection; peak area is proportional to ion concentration.
The anions chloride, nitrate, citrate, phosphate, and bicarbonate were separated in less than 4 minutes, and iothalamate in less than 5 minutes. Simultaneous quantitative analysis was performed for chloride, phosphate, and bicarbonate, demonstrating detection limits of 12 fmol for chloride, 12 fmol for phosphate, and 72 fmol for bicarbonate. A comparison between this method and a flow-through microfluorimeter analysis of chloride showed good agreement between the two micro-methods. Illustrative data from proximal and distal tubular fluid samples obtained by micropuncture (volume 30–70nL) are given, as are results from urine samples.
Results for chloride, phosphate, and bicarbonate in control material are in close agreement with the certified values, while values in tubular fluid are in accordance with previously published results.
This method provides a straightforward means of analyzing multiple anions in small volume biological samples. |
| doi_str_mv | 10.1111/j.1523-1755.2005.00089.x |
| format | Article |
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The study of renal tubular transport requires the ability to accurately measure ion concentrations in samples taken from single tubules. Sample collection and analysis are laborious, so methods allowing determination of multiple ion species in a small volume sample are advantageous. This article describes a method for the simultaneous analysis of anions at physiologic concentrations in nanoliter volumes of tubular fluid.
The analysis is performed using capillary zone electrophoresis. Diluted samples are moved along a capillary by bulk transport and separated according to charge and size. Peaks corresponding to anions are obtained by ultraviolet (UV) detection; peak area is proportional to ion concentration.
The anions chloride, nitrate, citrate, phosphate, and bicarbonate were separated in less than 4 minutes, and iothalamate in less than 5 minutes. Simultaneous quantitative analysis was performed for chloride, phosphate, and bicarbonate, demonstrating detection limits of 12 fmol for chloride, 12 fmol for phosphate, and 72 fmol for bicarbonate. A comparison between this method and a flow-through microfluorimeter analysis of chloride showed good agreement between the two micro-methods. Illustrative data from proximal and distal tubular fluid samples obtained by micropuncture (volume 30–70nL) are given, as are results from urine samples.
Results for chloride, phosphate, and bicarbonate in control material are in close agreement with the certified values, while values in tubular fluid are in accordance with previously published results.
This method provides a straightforward means of analyzing multiple anions in small volume biological samples.</description><identifier>ISSN: 0085-2538</identifier><identifier>EISSN: 1523-1755</identifier><identifier>DOI: 10.1111/j.1523-1755.2005.00089.x</identifier><identifier>PMID: 15610262</identifier><identifier>CODEN: KDYIA5</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Anions - analysis ; Bicarbonates - analysis ; Biological and medical sciences ; biological fluid measurement ; Body Fluids - chemistry ; capillary electrophoresis ; chloride ; Chlorides - analysis ; Electrophoresis, Capillary ; Iothalamic Acid - analysis ; Kidney Tubules - chemistry ; Medical sciences ; micropuncture ; Nanotechnology - instrumentation ; Nanotechnology - methods ; Nephrology. Urinary tract diseases ; phosphate ; Phosphates - analysis ; Rats ; renal tubule ; Reproducibility of Results ; Spectrophotometry, Ultraviolet</subject><ispartof>Kidney international, 2005-01, Vol.67 (1), p.357-363</ispartof><rights>2005 International Society of Nephrology</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Jan 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-63582ef9c620dbc1263eec0a4a8a2b14b3e433aebfcedae2d92af584c8f39abe3</citedby><cites>FETCH-LOGICAL-c479t-63582ef9c620dbc1263eec0a4a8a2b14b3e433aebfcedae2d92af584c8f39abe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16476835$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15610262$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Faria, Nuno J.R.</creatorcontrib><creatorcontrib>Dobbie, Hamish</creatorcontrib><creatorcontrib>Slater, Jonathan M.</creatorcontrib><creatorcontrib>Shirley, David G.</creatorcontrib><creatorcontrib>Stocking, Christopher J.</creatorcontrib><creatorcontrib>Unwin, Robert J.</creatorcontrib><title>Simultaneous determination of anions in nanoliter volumes</title><title>Kidney international</title><addtitle>Kidney Int</addtitle><description>Simultaneous determination of anions in nanoliter volumes.
The study of renal tubular transport requires the ability to accurately measure ion concentrations in samples taken from single tubules. Sample collection and analysis are laborious, so methods allowing determination of multiple ion species in a small volume sample are advantageous. This article describes a method for the simultaneous analysis of anions at physiologic concentrations in nanoliter volumes of tubular fluid.
The analysis is performed using capillary zone electrophoresis. Diluted samples are moved along a capillary by bulk transport and separated according to charge and size. Peaks corresponding to anions are obtained by ultraviolet (UV) detection; peak area is proportional to ion concentration.
The anions chloride, nitrate, citrate, phosphate, and bicarbonate were separated in less than 4 minutes, and iothalamate in less than 5 minutes. Simultaneous quantitative analysis was performed for chloride, phosphate, and bicarbonate, demonstrating detection limits of 12 fmol for chloride, 12 fmol for phosphate, and 72 fmol for bicarbonate. A comparison between this method and a flow-through microfluorimeter analysis of chloride showed good agreement between the two micro-methods. Illustrative data from proximal and distal tubular fluid samples obtained by micropuncture (volume 30–70nL) are given, as are results from urine samples.
Results for chloride, phosphate, and bicarbonate in control material are in close agreement with the certified values, while values in tubular fluid are in accordance with previously published results.
This method provides a straightforward means of analyzing multiple anions in small volume biological samples.</description><subject>Animals</subject><subject>Anions - analysis</subject><subject>Bicarbonates - analysis</subject><subject>Biological and medical sciences</subject><subject>biological fluid measurement</subject><subject>Body Fluids - chemistry</subject><subject>capillary electrophoresis</subject><subject>chloride</subject><subject>Chlorides - analysis</subject><subject>Electrophoresis, Capillary</subject><subject>Iothalamic Acid - analysis</subject><subject>Kidney Tubules - chemistry</subject><subject>Medical sciences</subject><subject>micropuncture</subject><subject>Nanotechnology - instrumentation</subject><subject>Nanotechnology - methods</subject><subject>Nephrology. Urinary tract diseases</subject><subject>phosphate</subject><subject>Phosphates - analysis</subject><subject>Rats</subject><subject>renal tubule</subject><subject>Reproducibility of Results</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0085-2538</issn><issn>1523-1755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkF1LwzAUhoMobk7_ghRB71rz0XTppQ6_YOCFeh3S9BQy2mQm7Zj_3nQbCt6Ym4RznnN48yCUEJyReG5XGeGUpWTOeUYx5hnGWJTZ9ghNfxrHaBqLPKWciQk6C2G1gxg-RRPCC4JpQaeofDPd0PbKghtCUkMPvjNW9cbZxDWJsvEREmMTq6xrTWwnG9cOHYRzdNKoNsDF4Z6hj8eH98Vzunx9elncLVOdz8s-LRgXFJpSFxTXlSa0YAAaq1wJRSuSVwxyxhRUjYZaAa1Lqhouci0aVqoK2Azd7PeuvfscIPSyM0FD2-4zy2LOWE5FHsGrP-DKDd7GbJISTCjhJYuQ2EPauxA8NHLtTaf8lyRYjm7lSo4K5ahQjm7lTpvcxtHLw_6h6qD-HTzIjMD1AVBBq7bxymoTfrkinxeC8cjd7zmI2jYGvAzagI3_Nx50L2tn_k_zDUzCmEY</recordid><startdate>200501</startdate><enddate>200501</enddate><creator>Faria, Nuno J.R.</creator><creator>Dobbie, Hamish</creator><creator>Slater, Jonathan M.</creator><creator>Shirley, David G.</creator><creator>Stocking, Christopher J.</creator><creator>Unwin, Robert J.</creator><general>Elsevier Inc</general><general>Nature Publishing</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>200501</creationdate><title>Simultaneous determination of anions in nanoliter volumes</title><author>Faria, Nuno J.R. ; Dobbie, Hamish ; Slater, Jonathan M. ; Shirley, David G. ; Stocking, Christopher J. ; Unwin, Robert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-63582ef9c620dbc1263eec0a4a8a2b14b3e433aebfcedae2d92af584c8f39abe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Anions - analysis</topic><topic>Bicarbonates - analysis</topic><topic>Biological and medical sciences</topic><topic>biological fluid measurement</topic><topic>Body Fluids - chemistry</topic><topic>capillary electrophoresis</topic><topic>chloride</topic><topic>Chlorides - analysis</topic><topic>Electrophoresis, Capillary</topic><topic>Iothalamic Acid - analysis</topic><topic>Kidney Tubules - chemistry</topic><topic>Medical sciences</topic><topic>micropuncture</topic><topic>Nanotechnology - instrumentation</topic><topic>Nanotechnology - methods</topic><topic>Nephrology. Urinary tract diseases</topic><topic>phosphate</topic><topic>Phosphates - analysis</topic><topic>Rats</topic><topic>renal tubule</topic><topic>Reproducibility of Results</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Faria, Nuno J.R.</creatorcontrib><creatorcontrib>Dobbie, Hamish</creatorcontrib><creatorcontrib>Slater, Jonathan M.</creatorcontrib><creatorcontrib>Shirley, David G.</creatorcontrib><creatorcontrib>Stocking, Christopher J.</creatorcontrib><creatorcontrib>Unwin, Robert J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Kidney international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Faria, Nuno J.R.</au><au>Dobbie, Hamish</au><au>Slater, Jonathan M.</au><au>Shirley, David G.</au><au>Stocking, Christopher J.</au><au>Unwin, Robert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of anions in nanoliter volumes</atitle><jtitle>Kidney international</jtitle><addtitle>Kidney Int</addtitle><date>2005-01</date><risdate>2005</risdate><volume>67</volume><issue>1</issue><spage>357</spage><epage>363</epage><pages>357-363</pages><issn>0085-2538</issn><eissn>1523-1755</eissn><coden>KDYIA5</coden><abstract>Simultaneous determination of anions in nanoliter volumes.
The study of renal tubular transport requires the ability to accurately measure ion concentrations in samples taken from single tubules. Sample collection and analysis are laborious, so methods allowing determination of multiple ion species in a small volume sample are advantageous. This article describes a method for the simultaneous analysis of anions at physiologic concentrations in nanoliter volumes of tubular fluid.
The analysis is performed using capillary zone electrophoresis. Diluted samples are moved along a capillary by bulk transport and separated according to charge and size. Peaks corresponding to anions are obtained by ultraviolet (UV) detection; peak area is proportional to ion concentration.
The anions chloride, nitrate, citrate, phosphate, and bicarbonate were separated in less than 4 minutes, and iothalamate in less than 5 minutes. Simultaneous quantitative analysis was performed for chloride, phosphate, and bicarbonate, demonstrating detection limits of 12 fmol for chloride, 12 fmol for phosphate, and 72 fmol for bicarbonate. A comparison between this method and a flow-through microfluorimeter analysis of chloride showed good agreement between the two micro-methods. Illustrative data from proximal and distal tubular fluid samples obtained by micropuncture (volume 30–70nL) are given, as are results from urine samples.
Results for chloride, phosphate, and bicarbonate in control material are in close agreement with the certified values, while values in tubular fluid are in accordance with previously published results.
This method provides a straightforward means of analyzing multiple anions in small volume biological samples.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>15610262</pmid><doi>10.1111/j.1523-1755.2005.00089.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Anions - analysis Bicarbonates - analysis Biological and medical sciences biological fluid measurement Body Fluids - chemistry capillary electrophoresis chloride Chlorides - analysis Electrophoresis, Capillary Iothalamic Acid - analysis Kidney Tubules - chemistry Medical sciences micropuncture Nanotechnology - instrumentation Nanotechnology - methods Nephrology. Urinary tract diseases phosphate Phosphates - analysis Rats renal tubule Reproducibility of Results Spectrophotometry, Ultraviolet |
| title | Simultaneous determination of anions in nanoliter volumes |
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