Intestinal Lipoprotein Overproduction, a Newly Recognized Component of Insulin Resistance, Is Ameliorated by the Insulin Sensitizer Rosiglitazone: Studies in the Fructose-Fed Syrian Golden Hamster
We investigated whether intestinal lipoprotein overproduction in a fructose-fed, insulin-resistant hamster model is prevented with insulin sensitization. Syrian Golden hamsters were fed either chow, 60% fructose for 5 wk, chow for 5 wk with the insulin sensitizer rosiglitazone added for the last 3 w...
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description | We investigated whether intestinal lipoprotein overproduction in a fructose-fed, insulin-resistant hamster model is prevented with insulin sensitization. Syrian Golden hamsters were fed either chow, 60% fructose for 5 wk, chow for 5 wk with the insulin sensitizer rosiglitazone added for the last 3 wk, or 60% fructose plus rosiglitazone. In vivo Triton studies showed a 2- to 3-fold increase in the large (Svedberg unit > 400) and smaller (Sf 100–400) triglyceride-rich lipoprotein particle apolipoprotein B48 (apoB48) but not triglyceride secretion with fructose feeding in the fasted state (P < 0.01) and partial normalization with rosiglitazone in fructose-fed hamsters. Ex vivo pulse-chase labeling of enterocytes confirmed the oversecretion of apoB48 lipoproteins with fructose feeding. Intestinal lipoprotein oversecretion was associated with increased expression of microsomal triglyceride transfer protein expression. With rosiglitazone treatment of fructose-fed hamsters, there was approximately 50% reduction in apoB48 secretion from primary cultured enterocytes and amelioration of the elevated microsomal triglyceride transfer protein mass and activity in fructose-fed hamsters. In contrast, in the postprandial state, the major differences between nutritional and drug intervention protocols were evident in triglyceride-rich lipoprotein triglyceride and not apoB48 secretion rates. The data suggest that intestinal lipoprotein overproduction can be ameliorated with the insulin sensitizer rosiglitazone. |
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Syrian Golden hamsters were fed either chow, 60% fructose for 5 wk, chow for 5 wk with the insulin sensitizer rosiglitazone added for the last 3 wk, or 60% fructose plus rosiglitazone. In vivo Triton studies showed a 2- to 3-fold increase in the large (Svedberg unit > 400) and smaller (Sf 100–400) triglyceride-rich lipoprotein particle apolipoprotein B48 (apoB48) but not triglyceride secretion with fructose feeding in the fasted state (P < 0.01) and partial normalization with rosiglitazone in fructose-fed hamsters. Ex vivo pulse-chase labeling of enterocytes confirmed the oversecretion of apoB48 lipoproteins with fructose feeding. Intestinal lipoprotein oversecretion was associated with increased expression of microsomal triglyceride transfer protein expression. With rosiglitazone treatment of fructose-fed hamsters, there was approximately 50% reduction in apoB48 secretion from primary cultured enterocytes and amelioration of the elevated microsomal triglyceride transfer protein mass and activity in fructose-fed hamsters. In contrast, in the postprandial state, the major differences between nutritional and drug intervention protocols were evident in triglyceride-rich lipoprotein triglyceride and not apoB48 secretion rates. The data suggest that intestinal lipoprotein overproduction can be ameliorated with the insulin sensitizer rosiglitazone.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/en.2004-1143</identifier><identifier>PMID: 15486228</identifier><identifier>CODEN: ENDOAO</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Animals ; Apolipoprotein B-100 ; Apolipoprotein B-48 ; Apolipoproteins B - blood ; Biological and medical sciences ; Carrier Proteins - metabolism ; Cells, Cultured ; Cricetinae ; Enterocytes ; Enterocytes - metabolism ; Fasting - blood ; Fructose ; Fundamental and applied biological sciences. Psychology ; Hamsters ; In vivo methods and tests ; Insulin ; Insulin resistance ; Insulin Resistance - physiology ; Intestine ; Intestines - cytology ; Intestines - metabolism ; Lipoproteins ; Lipoproteins - antagonists & inhibitors ; Lipoproteins - biosynthesis ; Mesocricetus ; Polyethylene Glycols - pharmacology ; Postprandial Period - physiology ; Proteins ; Rosiglitazone ; Secretion ; Thiazolidinediones - pharmacology ; Triglycerides ; Triglycerides - blood ; Vertebrates: endocrinology</subject><ispartof>Endocrinology (Philadelphia), 2005-01, Vol.146 (1), p.247-255</ispartof><rights>Copyright © 2005 by The Endocrine Society 2005</rights><rights>2005 INIST-CNRS</rights><rights>Copyright © 2005 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-9b7a8bbcdab8f4fc5c5315a5592317f30e858179df25999f024aa66590d9df223</citedby><cites>FETCH-LOGICAL-c527t-9b7a8bbcdab8f4fc5c5315a5592317f30e858179df25999f024aa66590d9df223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16444510$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15486228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lewis, Gary F</creatorcontrib><creatorcontrib>Uffelman, Kristine</creatorcontrib><creatorcontrib>Naples, Mark</creatorcontrib><creatorcontrib>Szeto, Linda</creatorcontrib><creatorcontrib>Haidari, Mehran</creatorcontrib><creatorcontrib>Adeli, Khosrow</creatorcontrib><title>Intestinal Lipoprotein Overproduction, a Newly Recognized Component of Insulin Resistance, Is Ameliorated by the Insulin Sensitizer Rosiglitazone: Studies in the Fructose-Fed Syrian Golden Hamster</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>We investigated whether intestinal lipoprotein overproduction in a fructose-fed, insulin-resistant hamster model is prevented with insulin sensitization. Syrian Golden hamsters were fed either chow, 60% fructose for 5 wk, chow for 5 wk with the insulin sensitizer rosiglitazone added for the last 3 wk, or 60% fructose plus rosiglitazone. In vivo Triton studies showed a 2- to 3-fold increase in the large (Svedberg unit > 400) and smaller (Sf 100–400) triglyceride-rich lipoprotein particle apolipoprotein B48 (apoB48) but not triglyceride secretion with fructose feeding in the fasted state (P < 0.01) and partial normalization with rosiglitazone in fructose-fed hamsters. Ex vivo pulse-chase labeling of enterocytes confirmed the oversecretion of apoB48 lipoproteins with fructose feeding. Intestinal lipoprotein oversecretion was associated with increased expression of microsomal triglyceride transfer protein expression. With rosiglitazone treatment of fructose-fed hamsters, there was approximately 50% reduction in apoB48 secretion from primary cultured enterocytes and amelioration of the elevated microsomal triglyceride transfer protein mass and activity in fructose-fed hamsters. In contrast, in the postprandial state, the major differences between nutritional and drug intervention protocols were evident in triglyceride-rich lipoprotein triglyceride and not apoB48 secretion rates. The data suggest that intestinal lipoprotein overproduction can be ameliorated with the insulin sensitizer rosiglitazone.</description><subject>Animals</subject><subject>Apolipoprotein B-100</subject><subject>Apolipoprotein B-48</subject><subject>Apolipoproteins B - blood</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - metabolism</subject><subject>Cells, Cultured</subject><subject>Cricetinae</subject><subject>Enterocytes</subject><subject>Enterocytes - metabolism</subject><subject>Fasting - blood</subject><subject>Fructose</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hamsters</subject><subject>In vivo methods and tests</subject><subject>Insulin</subject><subject>Insulin resistance</subject><subject>Insulin Resistance - physiology</subject><subject>Intestine</subject><subject>Intestines - cytology</subject><subject>Intestines - metabolism</subject><subject>Lipoproteins</subject><subject>Lipoproteins - antagonists & inhibitors</subject><subject>Lipoproteins - biosynthesis</subject><subject>Mesocricetus</subject><subject>Polyethylene Glycols - pharmacology</subject><subject>Postprandial Period - physiology</subject><subject>Proteins</subject><subject>Rosiglitazone</subject><subject>Secretion</subject><subject>Thiazolidinediones - pharmacology</subject><subject>Triglycerides</subject><subject>Triglycerides - blood</subject><subject>Vertebrates: endocrinology</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10U9v0zAYBvAIgVg3uHFGlhDj0gz_ieOE21TRrVLFpBbOkZO8GZ4SO_PrgLrPxwfDVSsqITglsX5-7LxPkrxh9IpxRj-CveKUZiljmXiWzFiZyVQxRZ8nM0qZSBXn6iw5R3yIn1mWiZfJGZNZkXNezJJfKxsAg7G6J2szutG7AMaSux_g43s7NcE4OyeafIGf_Y5soHH31jxBSxZuGJ0FG4jryMri1Md9G0CDQdsG5mSF5HqA3jivQ_T1joTv8EduwaIJMcmTjUNz35ugn2LeJ7INU2sASUT7DUsfL-EQ0mUM2e680ZbcuL4FS271gAH8q-RFp3uE18fnRfJt-fnr4jZd392sFtfrtJFchbSslS7quml1XXRZ18hGCia1lCUXTHWCQiELpsq247Isy47yTOs8lyVt92tcXCSXh9w4mccpjq0aDDbQ99qCm7DKlRCMlkWE7_6CD27yccZYCSaozLNcqajmB9V4h-ihq0ZvBu13FaPVvtsKbLXvttp3G_nbY-hUD9Ce8LHMCN4fgcZG952PNRg8uTy2LxmN7sPBuWn835Hp8UhxkGBb13hjYfSAePqbf170N9l2zGk</recordid><startdate>200501</startdate><enddate>200501</enddate><creator>Lewis, Gary F</creator><creator>Uffelman, Kristine</creator><creator>Naples, Mark</creator><creator>Szeto, Linda</creator><creator>Haidari, Mehran</creator><creator>Adeli, Khosrow</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200501</creationdate><title>Intestinal Lipoprotein Overproduction, a Newly Recognized Component of Insulin Resistance, Is Ameliorated by the Insulin Sensitizer Rosiglitazone: Studies in the Fructose-Fed Syrian Golden Hamster</title><author>Lewis, Gary F ; Uffelman, Kristine ; Naples, Mark ; Szeto, Linda ; Haidari, Mehran ; Adeli, Khosrow</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527t-9b7a8bbcdab8f4fc5c5315a5592317f30e858179df25999f024aa66590d9df223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Apolipoprotein B-100</topic><topic>Apolipoprotein B-48</topic><topic>Apolipoproteins B - blood</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - metabolism</topic><topic>Cells, Cultured</topic><topic>Cricetinae</topic><topic>Enterocytes</topic><topic>Enterocytes - metabolism</topic><topic>Fasting - blood</topic><topic>Fructose</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hamsters</topic><topic>In vivo methods and tests</topic><topic>Insulin</topic><topic>Insulin resistance</topic><topic>Insulin Resistance - physiology</topic><topic>Intestine</topic><topic>Intestines - cytology</topic><topic>Intestines - metabolism</topic><topic>Lipoproteins</topic><topic>Lipoproteins - antagonists & inhibitors</topic><topic>Lipoproteins - biosynthesis</topic><topic>Mesocricetus</topic><topic>Polyethylene Glycols - pharmacology</topic><topic>Postprandial Period - physiology</topic><topic>Proteins</topic><topic>Rosiglitazone</topic><topic>Secretion</topic><topic>Thiazolidinediones - pharmacology</topic><topic>Triglycerides</topic><topic>Triglycerides - blood</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lewis, Gary F</creatorcontrib><creatorcontrib>Uffelman, Kristine</creatorcontrib><creatorcontrib>Naples, Mark</creatorcontrib><creatorcontrib>Szeto, Linda</creatorcontrib><creatorcontrib>Haidari, Mehran</creatorcontrib><creatorcontrib>Adeli, Khosrow</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lewis, Gary F</au><au>Uffelman, Kristine</au><au>Naples, Mark</au><au>Szeto, Linda</au><au>Haidari, Mehran</au><au>Adeli, Khosrow</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intestinal Lipoprotein Overproduction, a Newly Recognized Component of Insulin Resistance, Is Ameliorated by the Insulin Sensitizer Rosiglitazone: Studies in the Fructose-Fed Syrian Golden Hamster</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>2005-01</date><risdate>2005</risdate><volume>146</volume><issue>1</issue><spage>247</spage><epage>255</epage><pages>247-255</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><coden>ENDOAO</coden><abstract>We investigated whether intestinal lipoprotein overproduction in a fructose-fed, insulin-resistant hamster model is prevented with insulin sensitization. Syrian Golden hamsters were fed either chow, 60% fructose for 5 wk, chow for 5 wk with the insulin sensitizer rosiglitazone added for the last 3 wk, or 60% fructose plus rosiglitazone. In vivo Triton studies showed a 2- to 3-fold increase in the large (Svedberg unit > 400) and smaller (Sf 100–400) triglyceride-rich lipoprotein particle apolipoprotein B48 (apoB48) but not triglyceride secretion with fructose feeding in the fasted state (P < 0.01) and partial normalization with rosiglitazone in fructose-fed hamsters. Ex vivo pulse-chase labeling of enterocytes confirmed the oversecretion of apoB48 lipoproteins with fructose feeding. Intestinal lipoprotein oversecretion was associated with increased expression of microsomal triglyceride transfer protein expression. With rosiglitazone treatment of fructose-fed hamsters, there was approximately 50% reduction in apoB48 secretion from primary cultured enterocytes and amelioration of the elevated microsomal triglyceride transfer protein mass and activity in fructose-fed hamsters. In contrast, in the postprandial state, the major differences between nutritional and drug intervention protocols were evident in triglyceride-rich lipoprotein triglyceride and not apoB48 secretion rates. The data suggest that intestinal lipoprotein overproduction can be ameliorated with the insulin sensitizer rosiglitazone.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>15486228</pmid><doi>10.1210/en.2004-1143</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
subjects | Animals Apolipoprotein B-100 Apolipoprotein B-48 Apolipoproteins B - blood Biological and medical sciences Carrier Proteins - metabolism Cells, Cultured Cricetinae Enterocytes Enterocytes - metabolism Fasting - blood Fructose Fundamental and applied biological sciences. Psychology Hamsters In vivo methods and tests Insulin Insulin resistance Insulin Resistance - physiology Intestine Intestines - cytology Intestines - metabolism Lipoproteins Lipoproteins - antagonists & inhibitors Lipoproteins - biosynthesis Mesocricetus Polyethylene Glycols - pharmacology Postprandial Period - physiology Proteins Rosiglitazone Secretion Thiazolidinediones - pharmacology Triglycerides Triglycerides - blood Vertebrates: endocrinology |
title | Intestinal Lipoprotein Overproduction, a Newly Recognized Component of Insulin Resistance, Is Ameliorated by the Insulin Sensitizer Rosiglitazone: Studies in the Fructose-Fed Syrian Golden Hamster |
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