Thyrotropin-releasing Hormone (TRH) specific interaction between amino terminus of P-Lim and CREB binding protein (CBP)
P-Lim (Lhx3a) is a LIM homeodomain transcription factor essential for pituitary development and motor neuron specification in mice. The Lhx3 gene encodes two isoforms, which differ in their amino (N) termini, Lhx3a and 3b. The P-Lim DNA binding site on the glycoprotein hormone alpha subunit (α-GSU)...
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| Veröffentlicht in: | Molecular and cellular endocrinology 2005-01, Vol.229 (1), p.11-20 |
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| container_title | Molecular and cellular endocrinology |
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| creator | Hashimoto, Koshi Yamada, Masanobu Monden, Tsuyoshi Satoh, Teturou Wondisford, Fredric E. Mori, Masatomo |
| description | P-Lim (Lhx3a) is a LIM homeodomain transcription factor essential for pituitary development and motor neuron specification in mice. The
Lhx3 gene encodes two isoforms, which differ in their amino (N) termini, Lhx3a and 3b. The P-Lim DNA binding site on the glycoprotein hormone alpha subunit (α-GSU) gene promoter is conserved in mammals. P-Lim plays a pivotal role in mediating thyrotropin-releasing hormone (TRH) signaling by binding CREB binding protein (CBP), as we have reported previously. Here, we demonstrate that P-Lim (Lhx3a) but not Lhx3b can mediate TRH signaling and bind to CBP. Moreover, TRH specifically induces P-Lim–CBP binding through the N-termini of P-Lim. We also found that the protein kinase C (PKC) phosphorylation site within the N-terminus of P-Lim is responsible for the P-Lim–CBP binding suggesting that the TRH signaling pathway phosphorylates P-Lim. These studies have elucidated the molecular mechanism by which TRH stimulates α-GSU gene expression. |
| doi_str_mv | 10.1016/j.mce.2004.10.005 |
| format | Article |
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Lhx3 gene encodes two isoforms, which differ in their amino (N) termini, Lhx3a and 3b. The P-Lim DNA binding site on the glycoprotein hormone alpha subunit (α-GSU) gene promoter is conserved in mammals. P-Lim plays a pivotal role in mediating thyrotropin-releasing hormone (TRH) signaling by binding CREB binding protein (CBP), as we have reported previously. Here, we demonstrate that P-Lim (Lhx3a) but not Lhx3b can mediate TRH signaling and bind to CBP. Moreover, TRH specifically induces P-Lim–CBP binding through the N-termini of P-Lim. We also found that the protein kinase C (PKC) phosphorylation site within the N-terminus of P-Lim is responsible for the P-Lim–CBP binding suggesting that the TRH signaling pathway phosphorylates P-Lim. These studies have elucidated the molecular mechanism by which TRH stimulates α-GSU gene expression.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/j.mce.2004.10.005</identifier><identifier>PMID: 15607524</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; CBP ; Chromatin Immunoprecipitation ; CREB-Binding Protein ; Glycoprotein hormone alpha subunit (GSU) gene ; Glycoprotein Hormones, alpha Subunit - genetics ; Homeodomain Proteins - metabolism ; Humans ; LIM-Homeodomain Proteins ; Luciferases - metabolism ; Mice ; Nuclear Proteins - metabolism ; P-Lim (Lhx3a) ; Phosphorylation ; Promoter Regions, Genetic - genetics ; Protein Kinase C - metabolism ; Thyrotropin-Releasing Hormone - metabolism ; Trans-Activators - metabolism ; Transcription Factors ; Transcription, Genetic ; Transcriptional Activation ; TRH ; Two-Hybrid System Techniques</subject><ispartof>Molecular and cellular endocrinology, 2005-01, Vol.229 (1), p.11-20</ispartof><rights>2004 Elsevier Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-6fdfc55fd6debd93333c3dbb6c059a4602b9d50ca86f445a6bdc20502a3684cf3</citedby><cites>FETCH-LOGICAL-c448t-6fdfc55fd6debd93333c3dbb6c059a4602b9d50ca86f445a6bdc20502a3684cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S030372070400396X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15607524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hashimoto, Koshi</creatorcontrib><creatorcontrib>Yamada, Masanobu</creatorcontrib><creatorcontrib>Monden, Tsuyoshi</creatorcontrib><creatorcontrib>Satoh, Teturou</creatorcontrib><creatorcontrib>Wondisford, Fredric E.</creatorcontrib><creatorcontrib>Mori, Masatomo</creatorcontrib><title>Thyrotropin-releasing Hormone (TRH) specific interaction between amino terminus of P-Lim and CREB binding protein (CBP)</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>P-Lim (Lhx3a) is a LIM homeodomain transcription factor essential for pituitary development and motor neuron specification in mice. The
Lhx3 gene encodes two isoforms, which differ in their amino (N) termini, Lhx3a and 3b. The P-Lim DNA binding site on the glycoprotein hormone alpha subunit (α-GSU) gene promoter is conserved in mammals. P-Lim plays a pivotal role in mediating thyrotropin-releasing hormone (TRH) signaling by binding CREB binding protein (CBP), as we have reported previously. Here, we demonstrate that P-Lim (Lhx3a) but not Lhx3b can mediate TRH signaling and bind to CBP. Moreover, TRH specifically induces P-Lim–CBP binding through the N-termini of P-Lim. We also found that the protein kinase C (PKC) phosphorylation site within the N-terminus of P-Lim is responsible for the P-Lim–CBP binding suggesting that the TRH signaling pathway phosphorylates P-Lim. These studies have elucidated the molecular mechanism by which TRH stimulates α-GSU gene expression.</description><subject>Animals</subject><subject>CBP</subject><subject>Chromatin Immunoprecipitation</subject><subject>CREB-Binding Protein</subject><subject>Glycoprotein hormone alpha subunit (GSU) gene</subject><subject>Glycoprotein Hormones, alpha Subunit - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>LIM-Homeodomain Proteins</subject><subject>Luciferases - metabolism</subject><subject>Mice</subject><subject>Nuclear Proteins - metabolism</subject><subject>P-Lim (Lhx3a)</subject><subject>Phosphorylation</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein Kinase C - metabolism</subject><subject>Thyrotropin-Releasing Hormone - metabolism</subject><subject>Trans-Activators - metabolism</subject><subject>Transcription Factors</subject><subject>Transcription, Genetic</subject><subject>Transcriptional Activation</subject><subject>TRH</subject><subject>Two-Hybrid System Techniques</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFqGzEQhkVpadykD9BL0akkh3VGu5J2TU-NSeqAoSG4Z6GVRq2MV3KldUPevlpsyK3RZaThm39AHyGfGMwZMHm9nQ8G5zUAL-85gHhDZqxr66oD0b4lM2igqdoa2jPyIectALSi7t6TMybkdOUz8rT5_ZzimOLehyrhDnX24RddxTTEgPRy87i6onmPxjtvqA8jJm1GHwPtcXxCDFQPPkRa-qUeMo2OPlRrP1AdLF0-3t7Q3gc7Ze7LHvSBXi5vHq4uyDundxk_nuo5-Xl3u1muqvWP7_fLb-vKcN6NlXTWGSGclRZ7u2jKMY3te2lALDSXUPcLK8DoTjrOhZa9NTUIqHUjO25cc06-HHPL9j8HzKMafDa42-mA8ZCVbJsGeM1eBVk7cS0vIDuCJsWcEzq1T37Q6VkxUJMWtVVFi5q0TK2ipcx8PoUf-gHty8TJQwG-HgEsf_HXY1LZeAwGrU9oRmWj_0_8PzD6nX0</recordid><startdate>20050114</startdate><enddate>20050114</enddate><creator>Hashimoto, Koshi</creator><creator>Yamada, Masanobu</creator><creator>Monden, Tsuyoshi</creator><creator>Satoh, Teturou</creator><creator>Wondisford, Fredric E.</creator><creator>Mori, Masatomo</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20050114</creationdate><title>Thyrotropin-releasing Hormone (TRH) specific interaction between amino terminus of P-Lim and CREB binding protein (CBP)</title><author>Hashimoto, Koshi ; Yamada, Masanobu ; Monden, Tsuyoshi ; Satoh, Teturou ; Wondisford, Fredric E. ; Mori, Masatomo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-6fdfc55fd6debd93333c3dbb6c059a4602b9d50ca86f445a6bdc20502a3684cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>CBP</topic><topic>Chromatin Immunoprecipitation</topic><topic>CREB-Binding Protein</topic><topic>Glycoprotein hormone alpha subunit (GSU) gene</topic><topic>Glycoprotein Hormones, alpha Subunit - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>LIM-Homeodomain Proteins</topic><topic>Luciferases - metabolism</topic><topic>Mice</topic><topic>Nuclear Proteins - metabolism</topic><topic>P-Lim (Lhx3a)</topic><topic>Phosphorylation</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein Kinase C - metabolism</topic><topic>Thyrotropin-Releasing Hormone - metabolism</topic><topic>Trans-Activators - metabolism</topic><topic>Transcription Factors</topic><topic>Transcription, Genetic</topic><topic>Transcriptional Activation</topic><topic>TRH</topic><topic>Two-Hybrid System Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hashimoto, Koshi</creatorcontrib><creatorcontrib>Yamada, Masanobu</creatorcontrib><creatorcontrib>Monden, Tsuyoshi</creatorcontrib><creatorcontrib>Satoh, Teturou</creatorcontrib><creatorcontrib>Wondisford, Fredric E.</creatorcontrib><creatorcontrib>Mori, Masatomo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hashimoto, Koshi</au><au>Yamada, Masanobu</au><au>Monden, Tsuyoshi</au><au>Satoh, Teturou</au><au>Wondisford, Fredric E.</au><au>Mori, Masatomo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thyrotropin-releasing Hormone (TRH) specific interaction between amino terminus of P-Lim and CREB binding protein (CBP)</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>2005-01-14</date><risdate>2005</risdate><volume>229</volume><issue>1</issue><spage>11</spage><epage>20</epage><pages>11-20</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>P-Lim (Lhx3a) is a LIM homeodomain transcription factor essential for pituitary development and motor neuron specification in mice. The
Lhx3 gene encodes two isoforms, which differ in their amino (N) termini, Lhx3a and 3b. The P-Lim DNA binding site on the glycoprotein hormone alpha subunit (α-GSU) gene promoter is conserved in mammals. P-Lim plays a pivotal role in mediating thyrotropin-releasing hormone (TRH) signaling by binding CREB binding protein (CBP), as we have reported previously. Here, we demonstrate that P-Lim (Lhx3a) but not Lhx3b can mediate TRH signaling and bind to CBP. Moreover, TRH specifically induces P-Lim–CBP binding through the N-termini of P-Lim. We also found that the protein kinase C (PKC) phosphorylation site within the N-terminus of P-Lim is responsible for the P-Lim–CBP binding suggesting that the TRH signaling pathway phosphorylates P-Lim. These studies have elucidated the molecular mechanism by which TRH stimulates α-GSU gene expression.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>15607524</pmid><doi>10.1016/j.mce.2004.10.005</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals CBP Chromatin Immunoprecipitation CREB-Binding Protein Glycoprotein hormone alpha subunit (GSU) gene Glycoprotein Hormones, alpha Subunit - genetics Homeodomain Proteins - metabolism Humans LIM-Homeodomain Proteins Luciferases - metabolism Mice Nuclear Proteins - metabolism P-Lim (Lhx3a) Phosphorylation Promoter Regions, Genetic - genetics Protein Kinase C - metabolism Thyrotropin-Releasing Hormone - metabolism Trans-Activators - metabolism Transcription Factors Transcription, Genetic Transcriptional Activation TRH Two-Hybrid System Techniques |
| title | Thyrotropin-releasing Hormone (TRH) specific interaction between amino terminus of P-Lim and CREB binding protein (CBP) |
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