Clostridium tagluense sp. nov., a psychrotolerant, anaerobic, spore-forming bacterium from permafrost
1 Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation 2 Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federa...
Gespeichert in:
| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2009-06, Vol.59 (6), p.1421-1426 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1426 |
|---|---|
| container_issue | 6 |
| container_start_page | 1421 |
| container_title | International journal of systematic and evolutionary microbiology |
| container_volume | 59 |
| creator | Suetin, Stanislav V Shcherbakova, Viktoria A Chuvilskaya, Natalya A Rivkina, Elisaveta M Suzina, Natalya E Lysenko, Anatoliy M Gilichinsky, David A |
| description | 1 Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation
2 Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation
3 Winogradsky Institute of Microbiology, Russian Academy of Sciences, 7/2 Prospekt 60-let Oktyabrya, Moscow 117811, Russian Federation
Correspondence Stanislav V. Suetin suetin{at}ibpm.pushchino.ru
A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121 T ) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of the 16S rRNA gene sequence of strain A121 T showed its affiliation with the group of psychrophilic and psychrotolerant members of cluster I of the genus Clostridium , Clostridium bowmanii DSM 14206 T being the closest relative (sequence similarity 98.5 %). Levels of DNA–DNA relatedness between strain A121 T and the type strains of phylogenetically related species ranged from 33 to 52 %. Strain A121 T grew in PY broth at temperatures between 4 and 28 °C (optimum 15–20 °C), at pH 6.0–8.0 (optimum pH 6.5–7.2) and in NaCl concentrations of 0–10.0 % (optimum 0–2.0 %). The strain utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose, fructose, trehalose, maltose and starch; it also hydrolysed gelatin. Predominant fatty acids were C 16 : 1 cis 9, C 16 : 1 cis 9 DMA, C 16 : 0 and C 14 : 0 . The DNA G+C content was 31.5 mol%. On the basis of its overall genotypic and phenotypic characteristics, strain A121 T is classified within a novel species of the genus Clostridium , Clostridium tagluense sp. nov. The type strain is A121 T (=VKM B-2369 T =DSM 17763 T ).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A121 T is DQ296031 .
Plots showing the effects of temperature and NaCl concentration on growth of strain A121 T , a comparison of the fatty acid profiles of strain A121 T and related type strains and an extended 16S rRNA gene sequence-based neighbour-joining tree are available as supplementary material with the online version of this paper. |
| doi_str_mv | 10.1099/ijs.0.002295-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67328087</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20076931</sourcerecordid><originalsourceid>FETCH-LOGICAL-c397t-5090725282b34401950226825dc62d3218d78f168713690d7705ab4b26c3a7593</originalsourceid><addsrcrecordid>eNqFkc1LxDAQxYMofl89Sk_iYVsnSZs0R1n8AsGLnkOapruRtqlJq-x_b-ouevQ0w-M3j5l5CF1gyDAIcWPfQwYZACGiSGEPHeOcsbQAmu__9oQdoZMQ3gGiAHCIjrCIKiX8GJll68LobW2nLhnVqp1MH0wShizp3We2SFQyhI1eeze61njVj1HqlfGusnoROedN2jjf2X6VVEqPxs9OjXddMhjfqdiF8QwdNKoN5nxXT9Hb_d3r8jF9fnl4Wt4-p5oKPsZVBXBSkJJUNM_hZ0vCSlLUmpGaElzWvGwwKzmmTEDNORSqyivCNFW8EPQUXW19B-8-JhNG2dmgTduq3rgpSMYpKaHk_4IEgDNBcQSzLajjHcGbRg7edspvJAY5JyBjAhLkNgEJceBy5zxVnan_8N3LI3C9BdZ2tf6y3siV6Tur54-62a0QkkmcE0y_AUs_jrg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20076931</pqid></control><display><type>article</type><title>Clostridium tagluense sp. nov., a psychrotolerant, anaerobic, spore-forming bacterium from permafrost</title><source>MEDLINE</source><source>Microbiology Society</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Suetin, Stanislav V ; Shcherbakova, Viktoria A ; Chuvilskaya, Natalya A ; Rivkina, Elisaveta M ; Suzina, Natalya E ; Lysenko, Anatoliy M ; Gilichinsky, David A</creator><creatorcontrib>Suetin, Stanislav V ; Shcherbakova, Viktoria A ; Chuvilskaya, Natalya A ; Rivkina, Elisaveta M ; Suzina, Natalya E ; Lysenko, Anatoliy M ; Gilichinsky, David A</creatorcontrib><description>1 Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation
2 Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation
3 Winogradsky Institute of Microbiology, Russian Academy of Sciences, 7/2 Prospekt 60-let Oktyabrya, Moscow 117811, Russian Federation
Correspondence Stanislav V. Suetin suetin{at}ibpm.pushchino.ru
A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121 T ) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of the 16S rRNA gene sequence of strain A121 T showed its affiliation with the group of psychrophilic and psychrotolerant members of cluster I of the genus Clostridium , Clostridium bowmanii DSM 14206 T being the closest relative (sequence similarity 98.5 %). Levels of DNA–DNA relatedness between strain A121 T and the type strains of phylogenetically related species ranged from 33 to 52 %. Strain A121 T grew in PY broth at temperatures between 4 and 28 °C (optimum 15–20 °C), at pH 6.0–8.0 (optimum pH 6.5–7.2) and in NaCl concentrations of 0–10.0 % (optimum 0–2.0 %). The strain utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose, fructose, trehalose, maltose and starch; it also hydrolysed gelatin. Predominant fatty acids were C 16 : 1 cis 9, C 16 : 1 cis 9 DMA, C 16 : 0 and C 14 : 0 . The DNA G+C content was 31.5 mol%. On the basis of its overall genotypic and phenotypic characteristics, strain A121 T is classified within a novel species of the genus Clostridium , Clostridium tagluense sp. nov. The type strain is A121 T (=VKM B-2369 T =DSM 17763 T ).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A121 T is DQ296031 .
Plots showing the effects of temperature and NaCl concentration on growth of strain A121 T , a comparison of the fatty acid profiles of strain A121 T and related type strains and an extended 16S rRNA gene sequence-based neighbour-joining tree are available as supplementary material with the online version of this paper.</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijs.0.002295-0</identifier><identifier>PMID: 19502327</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Anaerobiosis ; Arctic Regions ; Bacteria ; Bacterial Typing Techniques ; Canada ; Clostridium ; Clostridium - classification ; Clostridium - genetics ; Clostridium - isolation & purification ; Clostridium - physiology ; Cold Temperature ; DNA, Bacterial - analysis ; DNA, Ribosomal - analysis ; Fatty Acids - analysis ; Genotype ; Ice Cover - microbiology ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Phenotype ; Phylogeny ; Polymerase Chain Reaction - methods ; Polymorphism, Restriction Fragment Length ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Species Specificity ; Spores, Bacterial - physiology</subject><ispartof>International journal of systematic and evolutionary microbiology, 2009-06, Vol.59 (6), p.1421-1426</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-5090725282b34401950226825dc62d3218d78f168713690d7705ab4b26c3a7593</citedby><cites>FETCH-LOGICAL-c397t-5090725282b34401950226825dc62d3218d78f168713690d7705ab4b26c3a7593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,3748,3749,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19502327$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suetin, Stanislav V</creatorcontrib><creatorcontrib>Shcherbakova, Viktoria A</creatorcontrib><creatorcontrib>Chuvilskaya, Natalya A</creatorcontrib><creatorcontrib>Rivkina, Elisaveta M</creatorcontrib><creatorcontrib>Suzina, Natalya E</creatorcontrib><creatorcontrib>Lysenko, Anatoliy M</creatorcontrib><creatorcontrib>Gilichinsky, David A</creatorcontrib><title>Clostridium tagluense sp. nov., a psychrotolerant, anaerobic, spore-forming bacterium from permafrost</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>1 Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation
2 Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation
3 Winogradsky Institute of Microbiology, Russian Academy of Sciences, 7/2 Prospekt 60-let Oktyabrya, Moscow 117811, Russian Federation
Correspondence Stanislav V. Suetin suetin{at}ibpm.pushchino.ru
A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121 T ) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of the 16S rRNA gene sequence of strain A121 T showed its affiliation with the group of psychrophilic and psychrotolerant members of cluster I of the genus Clostridium , Clostridium bowmanii DSM 14206 T being the closest relative (sequence similarity 98.5 %). Levels of DNA–DNA relatedness between strain A121 T and the type strains of phylogenetically related species ranged from 33 to 52 %. Strain A121 T grew in PY broth at temperatures between 4 and 28 °C (optimum 15–20 °C), at pH 6.0–8.0 (optimum pH 6.5–7.2) and in NaCl concentrations of 0–10.0 % (optimum 0–2.0 %). The strain utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose, fructose, trehalose, maltose and starch; it also hydrolysed gelatin. Predominant fatty acids were C 16 : 1 cis 9, C 16 : 1 cis 9 DMA, C 16 : 0 and C 14 : 0 . The DNA G+C content was 31.5 mol%. On the basis of its overall genotypic and phenotypic characteristics, strain A121 T is classified within a novel species of the genus Clostridium , Clostridium tagluense sp. nov. The type strain is A121 T (=VKM B-2369 T =DSM 17763 T ).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A121 T is DQ296031 .
Plots showing the effects of temperature and NaCl concentration on growth of strain A121 T , a comparison of the fatty acid profiles of strain A121 T and related type strains and an extended 16S rRNA gene sequence-based neighbour-joining tree are available as supplementary material with the online version of this paper.</description><subject>Anaerobiosis</subject><subject>Arctic Regions</subject><subject>Bacteria</subject><subject>Bacterial Typing Techniques</subject><subject>Canada</subject><subject>Clostridium</subject><subject>Clostridium - classification</subject><subject>Clostridium - genetics</subject><subject>Clostridium - isolation & purification</subject><subject>Clostridium - physiology</subject><subject>Cold Temperature</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Ribosomal - analysis</subject><subject>Fatty Acids - analysis</subject><subject>Genotype</subject><subject>Ice Cover - microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Species Specificity</subject><subject>Spores, Bacterial - physiology</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1LxDAQxYMofl89Sk_iYVsnSZs0R1n8AsGLnkOapruRtqlJq-x_b-ouevQ0w-M3j5l5CF1gyDAIcWPfQwYZACGiSGEPHeOcsbQAmu__9oQdoZMQ3gGiAHCIjrCIKiX8GJll68LobW2nLhnVqp1MH0wShizp3We2SFQyhI1eeze61njVj1HqlfGusnoROedN2jjf2X6VVEqPxs9OjXddMhjfqdiF8QwdNKoN5nxXT9Hb_d3r8jF9fnl4Wt4-p5oKPsZVBXBSkJJUNM_hZ0vCSlLUmpGaElzWvGwwKzmmTEDNORSqyivCNFW8EPQUXW19B-8-JhNG2dmgTduq3rgpSMYpKaHk_4IEgDNBcQSzLajjHcGbRg7edspvJAY5JyBjAhLkNgEJceBy5zxVnan_8N3LI3C9BdZ2tf6y3siV6Tur54-62a0QkkmcE0y_AUs_jrg</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Suetin, Stanislav V</creator><creator>Shcherbakova, Viktoria A</creator><creator>Chuvilskaya, Natalya A</creator><creator>Rivkina, Elisaveta M</creator><creator>Suzina, Natalya E</creator><creator>Lysenko, Anatoliy M</creator><creator>Gilichinsky, David A</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>Clostridium tagluense sp. nov., a psychrotolerant, anaerobic, spore-forming bacterium from permafrost</title><author>Suetin, Stanislav V ; Shcherbakova, Viktoria A ; Chuvilskaya, Natalya A ; Rivkina, Elisaveta M ; Suzina, Natalya E ; Lysenko, Anatoliy M ; Gilichinsky, David A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-5090725282b34401950226825dc62d3218d78f168713690d7705ab4b26c3a7593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Anaerobiosis</topic><topic>Arctic Regions</topic><topic>Bacteria</topic><topic>Bacterial Typing Techniques</topic><topic>Canada</topic><topic>Clostridium</topic><topic>Clostridium - classification</topic><topic>Clostridium - genetics</topic><topic>Clostridium - isolation & purification</topic><topic>Clostridium - physiology</topic><topic>Cold Temperature</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Ribosomal - analysis</topic><topic>Fatty Acids - analysis</topic><topic>Genotype</topic><topic>Ice Cover - microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Phenotype</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Species Specificity</topic><topic>Spores, Bacterial - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suetin, Stanislav V</creatorcontrib><creatorcontrib>Shcherbakova, Viktoria A</creatorcontrib><creatorcontrib>Chuvilskaya, Natalya A</creatorcontrib><creatorcontrib>Rivkina, Elisaveta M</creatorcontrib><creatorcontrib>Suzina, Natalya E</creatorcontrib><creatorcontrib>Lysenko, Anatoliy M</creatorcontrib><creatorcontrib>Gilichinsky, David A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suetin, Stanislav V</au><au>Shcherbakova, Viktoria A</au><au>Chuvilskaya, Natalya A</au><au>Rivkina, Elisaveta M</au><au>Suzina, Natalya E</au><au>Lysenko, Anatoliy M</au><au>Gilichinsky, David A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clostridium tagluense sp. nov., a psychrotolerant, anaerobic, spore-forming bacterium from permafrost</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>59</volume><issue>6</issue><spage>1421</spage><epage>1426</epage><pages>1421-1426</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>1 Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation
2 Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation
3 Winogradsky Institute of Microbiology, Russian Academy of Sciences, 7/2 Prospekt 60-let Oktyabrya, Moscow 117811, Russian Federation
Correspondence Stanislav V. Suetin suetin{at}ibpm.pushchino.ru
A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121 T ) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of the 16S rRNA gene sequence of strain A121 T showed its affiliation with the group of psychrophilic and psychrotolerant members of cluster I of the genus Clostridium , Clostridium bowmanii DSM 14206 T being the closest relative (sequence similarity 98.5 %). Levels of DNA–DNA relatedness between strain A121 T and the type strains of phylogenetically related species ranged from 33 to 52 %. Strain A121 T grew in PY broth at temperatures between 4 and 28 °C (optimum 15–20 °C), at pH 6.0–8.0 (optimum pH 6.5–7.2) and in NaCl concentrations of 0–10.0 % (optimum 0–2.0 %). The strain utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose, fructose, trehalose, maltose and starch; it also hydrolysed gelatin. Predominant fatty acids were C 16 : 1 cis 9, C 16 : 1 cis 9 DMA, C 16 : 0 and C 14 : 0 . The DNA G+C content was 31.5 mol%. On the basis of its overall genotypic and phenotypic characteristics, strain A121 T is classified within a novel species of the genus Clostridium , Clostridium tagluense sp. nov. The type strain is A121 T (=VKM B-2369 T =DSM 17763 T ).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A121 T is DQ296031 .
Plots showing the effects of temperature and NaCl concentration on growth of strain A121 T , a comparison of the fatty acid profiles of strain A121 T and related type strains and an extended 16S rRNA gene sequence-based neighbour-joining tree are available as supplementary material with the online version of this paper.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>19502327</pmid><doi>10.1099/ijs.0.002295-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1466-5026 |
| ispartof | International journal of systematic and evolutionary microbiology, 2009-06, Vol.59 (6), p.1421-1426 |
| issn | 1466-5026 1466-5034 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67328087 |
| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Anaerobiosis Arctic Regions Bacteria Bacterial Typing Techniques Canada Clostridium Clostridium - classification Clostridium - genetics Clostridium - isolation & purification Clostridium - physiology Cold Temperature DNA, Bacterial - analysis DNA, Ribosomal - analysis Fatty Acids - analysis Genotype Ice Cover - microbiology Molecular Sequence Data Nucleic Acid Hybridization Phenotype Phylogeny Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Species Specificity Spores, Bacterial - physiology |
| title | Clostridium tagluense sp. nov., a psychrotolerant, anaerobic, spore-forming bacterium from permafrost |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-07T00%3A39%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clostridium%20tagluense%20sp.%20nov.,%20a%20psychrotolerant,%20anaerobic,%20spore-forming%20bacterium%20from%20permafrost&rft.jtitle=International%20journal%20of%20systematic%20and%20evolutionary%20microbiology&rft.au=Suetin,%20Stanislav%20V&rft.date=2009-06-01&rft.volume=59&rft.issue=6&rft.spage=1421&rft.epage=1426&rft.pages=1421-1426&rft.issn=1466-5026&rft.eissn=1466-5034&rft_id=info:doi/10.1099/ijs.0.002295-0&rft_dat=%3Cproquest_cross%3E20076931%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20076931&rft_id=info:pmid/19502327&rfr_iscdi=true |