Clostridium tagluense sp. nov., a psychrotolerant, anaerobic, spore-forming bacterium from permafrost

1 Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation 2 Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federa...

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Veröffentlicht in:International journal of systematic and evolutionary microbiology 2009-06, Vol.59 (6), p.1421-1426
Hauptverfasser: Suetin, Stanislav V, Shcherbakova, Viktoria A, Chuvilskaya, Natalya A, Rivkina, Elisaveta M, Suzina, Natalya E, Lysenko, Anatoliy M, Gilichinsky, David A
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container_title International journal of systematic and evolutionary microbiology
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creator Suetin, Stanislav V
Shcherbakova, Viktoria A
Chuvilskaya, Natalya A
Rivkina, Elisaveta M
Suzina, Natalya E
Lysenko, Anatoliy M
Gilichinsky, David A
description 1 Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation 2 Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation 3 Winogradsky Institute of Microbiology, Russian Academy of Sciences, 7/2 Prospekt 60-let Oktyabrya, Moscow 117811, Russian Federation Correspondence Stanislav V. Suetin suetin{at}ibpm.pushchino.ru A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121 T ) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of the 16S rRNA gene sequence of strain A121 T showed its affiliation with the group of psychrophilic and psychrotolerant members of cluster I of the genus Clostridium , Clostridium bowmanii DSM 14206 T being the closest relative (sequence similarity 98.5 %). Levels of DNA–DNA relatedness between strain A121 T and the type strains of phylogenetically related species ranged from 33 to 52 %. Strain A121 T grew in PY broth at temperatures between 4 and 28 °C (optimum 15–20 °C), at pH 6.0–8.0 (optimum pH 6.5–7.2) and in NaCl concentrations of 0–10.0 % (optimum 0–2.0 %). The strain utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose, fructose, trehalose, maltose and starch; it also hydrolysed gelatin. Predominant fatty acids were C 16 : 1 cis 9, C 16 : 1 cis 9 DMA, C 16 : 0 and C 14 : 0 . The DNA G+C content was 31.5 mol%. On the basis of its overall genotypic and phenotypic characteristics, strain A121 T is classified within a novel species of the genus Clostridium , Clostridium tagluense sp. nov. The type strain is A121 T (=VKM B-2369 T =DSM 17763 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A121 T is DQ296031 . Plots showing the effects of temperature and NaCl concentration on growth of strain A121 T , a comparison of the fatty acid profiles of strain A121 T and related type strains and an extended 16S rRNA gene sequence-based neighbour-joining tree are available as supplementary material with the online version of this paper.
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Suetin suetin{at}ibpm.pushchino.ru A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121 T ) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of the 16S rRNA gene sequence of strain A121 T showed its affiliation with the group of psychrophilic and psychrotolerant members of cluster I of the genus Clostridium , Clostridium bowmanii DSM 14206 T being the closest relative (sequence similarity 98.5 %). Levels of DNA–DNA relatedness between strain A121 T and the type strains of phylogenetically related species ranged from 33 to 52 %. Strain A121 T grew in PY broth at temperatures between 4 and 28 °C (optimum 15–20 °C), at pH 6.0–8.0 (optimum pH 6.5–7.2) and in NaCl concentrations of 0–10.0 % (optimum 0–2.0 %). The strain utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose, fructose, trehalose, maltose and starch; it also hydrolysed gelatin. Predominant fatty acids were C 16 : 1 cis 9, C 16 : 1 cis 9 DMA, C 16 : 0 and C 14 : 0 . The DNA G+C content was 31.5 mol%. On the basis of its overall genotypic and phenotypic characteristics, strain A121 T is classified within a novel species of the genus Clostridium , Clostridium tagluense sp. nov. The type strain is A121 T (=VKM B-2369 T =DSM 17763 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A121 T is DQ296031 . 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Suetin suetin{at}ibpm.pushchino.ru A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121 T ) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of the 16S rRNA gene sequence of strain A121 T showed its affiliation with the group of psychrophilic and psychrotolerant members of cluster I of the genus Clostridium , Clostridium bowmanii DSM 14206 T being the closest relative (sequence similarity 98.5 %). Levels of DNA–DNA relatedness between strain A121 T and the type strains of phylogenetically related species ranged from 33 to 52 %. Strain A121 T grew in PY broth at temperatures between 4 and 28 °C (optimum 15–20 °C), at pH 6.0–8.0 (optimum pH 6.5–7.2) and in NaCl concentrations of 0–10.0 % (optimum 0–2.0 %). The strain utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose, fructose, trehalose, maltose and starch; it also hydrolysed gelatin. Predominant fatty acids were C 16 : 1 cis 9, C 16 : 1 cis 9 DMA, C 16 : 0 and C 14 : 0 . The DNA G+C content was 31.5 mol%. On the basis of its overall genotypic and phenotypic characteristics, strain A121 T is classified within a novel species of the genus Clostridium , Clostridium tagluense sp. nov. The type strain is A121 T (=VKM B-2369 T =DSM 17763 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A121 T is DQ296031 . 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Suetin suetin{at}ibpm.pushchino.ru A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121 T ) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of the 16S rRNA gene sequence of strain A121 T showed its affiliation with the group of psychrophilic and psychrotolerant members of cluster I of the genus Clostridium , Clostridium bowmanii DSM 14206 T being the closest relative (sequence similarity 98.5 %). Levels of DNA–DNA relatedness between strain A121 T and the type strains of phylogenetically related species ranged from 33 to 52 %. Strain A121 T grew in PY broth at temperatures between 4 and 28 °C (optimum 15–20 °C), at pH 6.0–8.0 (optimum pH 6.5–7.2) and in NaCl concentrations of 0–10.0 % (optimum 0–2.0 %). The strain utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose, fructose, trehalose, maltose and starch; it also hydrolysed gelatin. Predominant fatty acids were C 16 : 1 cis 9, C 16 : 1 cis 9 DMA, C 16 : 0 and C 14 : 0 . The DNA G+C content was 31.5 mol%. On the basis of its overall genotypic and phenotypic characteristics, strain A121 T is classified within a novel species of the genus Clostridium , Clostridium tagluense sp. nov. The type strain is A121 T (=VKM B-2369 T =DSM 17763 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A121 T is DQ296031 . Plots showing the effects of temperature and NaCl concentration on growth of strain A121 T , a comparison of the fatty acid profiles of strain A121 T and related type strains and an extended 16S rRNA gene sequence-based neighbour-joining tree are available as supplementary material with the online version of this paper.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>19502327</pmid><doi>10.1099/ijs.0.002295-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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source MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Anaerobiosis
Arctic Regions
Bacteria
Bacterial Typing Techniques
Canada
Clostridium
Clostridium - classification
Clostridium - genetics
Clostridium - isolation & purification
Clostridium - physiology
Cold Temperature
DNA, Bacterial - analysis
DNA, Ribosomal - analysis
Fatty Acids - analysis
Genotype
Ice Cover - microbiology
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Phylogeny
Polymerase Chain Reaction - methods
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Species Specificity
Spores, Bacterial - physiology
title Clostridium tagluense sp. nov., a psychrotolerant, anaerobic, spore-forming bacterium from permafrost
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