Expression, refolding, and purification of recombinant human granzyme B

Granzyme B (GrB) is a member of a family of serine proteases involved in cytotoxic T-lymphocyte-mediated killing of potentially harmful cells, where GrB induces apoptosis by cleavage of a limited number of substrates. To investigate the suitability of GrB as an enzyme for specific fusion protein cle...

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Veröffentlicht in:	Protein expression and purification 2005, Vol.39 (1), p.18-26
Hauptverfasser:	Lorentsen, Rikke H., Fynbo, Charlotte H., Thøgersen, Hans C., Etzerodt, Michael, Holtet, Thor L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cloning, Molecular Electrophoresis, Polyacrylamide Gel Granzyme B Granzymes Humans Kinetics Mutation Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Serine Endopeptidases - genetics Serine Endopeptidases - isolation & purification Serine Endopeptidases - metabolism Substrate Specificity
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