Atan1p--an extracellular tannase from the dimorphic yeast Arxula adeninivorans: molecular cloning of the ATAN1 gene and characterization of the recombinant enzyme

The tannase-encoding Arxula adeninivorans gene ATAN1 was isolated from genomic DNA by PCR, using as primers oligonucleotide sequences derived from peptides obtained after tryptic digestion of the purified tannase protein. The gene harbours an ORF of 1764 bp, encoding a 587-amino acid protein, preced...

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Veröffentlicht in:	Yeast (Chichester, England) England), 2009-06, Vol.26 (6), p.323-337
Hauptverfasser:	Böer, Erik, Bode, Rüdiger, Mock, Hans-Peter, Piontek, Michael, Kunze, Gotthard
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Arxula adeninivorans Aspergillus nidulans Aspergillus oryzae ATAN1 Carboxylic Ester Hydrolases - chemistry Carboxylic Ester Hydrolases - genetics Carboxylic Ester Hydrolases - metabolism Cloning, Molecular Fungal Proteins - chemistry Fungal Proteins - genetics Fungal Proteins - metabolism gallic acid Gallic Acid - metabolism Hydrogen-Ion Concentration Molecular Sequence Data Molecular Weight Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - metabolism Saccharomycetales - enzymology Saccharomycetales - genetics Saccharomycetales - growth & development Sequence Homology, Amino Acid tannase tannic acid Tannins - metabolism Temperature Transcriptional Activation yeast
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