Molecular Cloning and Developmental Expression of Corticotropin-Releasing Factor in the Chicken
We have characterized the structure of the chicken corticotropin-releasing factor (CRF) gene through cDNA cloning and genomic sequence analysis, and we analyzed the expression of CRF mRNA and peptide in the diencephalon of the chick throughout embryonic development. The structure of the chicken CRF...
Gespeichert in:
| Veröffentlicht in: | Endocrinology (Philadelphia) 2005-01, Vol.146 (1), p.301-308 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 308 |
|---|---|
| container_issue | 1 |
| container_start_page | 301 |
| container_title | Endocrinology (Philadelphia) |
| container_volume | 146 |
| creator | Vandenborne, Kristien De Groef, Bert Geelissen, Sofie M. E Boorse, Graham C Denver, Robert J Kühn, Eduard R Darras, Veerle M Van der Geyten, Serge |
| description | We have characterized the structure of the chicken corticotropin-releasing factor (CRF) gene through cDNA cloning and genomic sequence analysis, and we analyzed the expression of CRF mRNA and peptide in the diencephalon of the chick throughout embryonic development. The structure of the chicken CRF gene is similar to other vertebrate CRF genes and contains two exons and a single intron. The primary structure of the mature chicken CRF peptide is identical to human and rat CRF. This is the first archosaurian CRF gene to be characterized. We used RIAs to analyze CRF peptide content in the diencephalon and the median eminence and plasma corticosterone during the last week of embryonic development. We also developed a semiquantitative RT-PCR method to analyze the expression of CRF mRNA during the same period. CRF peptide content in the diencephalon increased, whereas peptide content in the ME decreased just before hatching, suggesting that release and biosynthesis are coupled. Plasma corticosterone concentration significantly increased between embryonic d 20 and the first day post hatch. By contrast, CRF mRNA levels in the diencephalon decreased just before hatching. Changes in CRF production just before hatching may be causally related to the regulation of the thyroid and interrenal axes at this stage of chicken development. |
| doi_str_mv | 10.1210/en.2004-0608 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67321391</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1210/en.2004-0608</oup_id><sourcerecordid>3130567617</sourcerecordid><originalsourceid>FETCH-LOGICAL-c527t-94c8f7552a72394ef927e0f551b7359fc74fd87f7c6a87fcf6a8241aba8c69b53</originalsourceid><addsrcrecordid>eNp1kd9rFDEQx4NY7LX65rMsiPXFrfmd3UdZWytUBNHnkMtNbGou2Sa7Uv97c72FA7FPXwY-M9-Z7yD0kuBzQgl-D_GcYsxbLHH3BK1Iz0WriMJP0QpjwlpFqTpGJ6Xc1pJzzp6hYyJY10kuV0h_SQHsHExuhpCijz8bEzfNR_gNIY1biJMJzcX9mKEUn2KTXDOkPHmbppxGH9tvEMCUXd-lsVPKjY_NdAPNcOPtL4jP0ZEzocCLRU_Rj8uL78NVe_310-fhw3VrBVVT23PbOSUENYqynoPrqQLshCBrxUTvrOJu0ymnrDRVrKtCOTFr01nZrwU7RWf7uWNOdzOUSW99sRCCiZDmoqVilLCeVPD1P-BtmnOsu2lGGBZSSaIq9W5P2ZxKyeD0mP3W5D-aYL2LXUPUu9j1LvaKv1qGzustbA7wknMF3iyAKdYEl020vhw4WR8jHnzf7rk0j49Ztosl25MQN8lmH-HhR4dr_rvoX4g_pyg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3130567617</pqid></control><display><type>article</type><title>Molecular Cloning and Developmental Expression of Corticotropin-Releasing Factor in the Chicken</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Vandenborne, Kristien ; De Groef, Bert ; Geelissen, Sofie M. E ; Boorse, Graham C ; Denver, Robert J ; Kühn, Eduard R ; Darras, Veerle M ; Van der Geyten, Serge</creator><creatorcontrib>Vandenborne, Kristien ; De Groef, Bert ; Geelissen, Sofie M. E ; Boorse, Graham C ; Denver, Robert J ; Kühn, Eduard R ; Darras, Veerle M ; Van der Geyten, Serge</creatorcontrib><description>We have characterized the structure of the chicken corticotropin-releasing factor (CRF) gene through cDNA cloning and genomic sequence analysis, and we analyzed the expression of CRF mRNA and peptide in the diencephalon of the chick throughout embryonic development. The structure of the chicken CRF gene is similar to other vertebrate CRF genes and contains two exons and a single intron. The primary structure of the mature chicken CRF peptide is identical to human and rat CRF. This is the first archosaurian CRF gene to be characterized. We used RIAs to analyze CRF peptide content in the diencephalon and the median eminence and plasma corticosterone during the last week of embryonic development. We also developed a semiquantitative RT-PCR method to analyze the expression of CRF mRNA during the same period. CRF peptide content in the diencephalon increased, whereas peptide content in the ME decreased just before hatching, suggesting that release and biosynthesis are coupled. Plasma corticosterone concentration significantly increased between embryonic d 20 and the first day post hatch. By contrast, CRF mRNA levels in the diencephalon decreased just before hatching. Changes in CRF production just before hatching may be causally related to the regulation of the thyroid and interrenal axes at this stage of chicken development.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/en.2004-0608</identifier><identifier>PMID: 15388646</identifier><identifier>CODEN: ENDOAO</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Aging - metabolism ; Amino Acid Sequence ; Animals ; Animals, Newborn - metabolism ; Base Sequence ; Biological and medical sciences ; Biosynthesis ; Chick Embryo - metabolism ; Chick Embryo - physiology ; Chickens ; Chickens - blood ; Chickens - genetics ; Chickens - growth & development ; Cloning ; Cloning, Molecular ; Corticosterone ; Corticosterone - blood ; Corticotropin-releasing hormone ; Corticotropin-Releasing Hormone - genetics ; Corticotropin-Releasing Hormone - metabolism ; Developmental stages ; Diencephalon ; Diencephalon - metabolism ; Embryogenesis ; Embryonic growth stage ; Exons ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Developmental ; Genomic analysis ; Hatching ; Median Eminence - metabolism ; Molecular Sequence Data ; Molecular Structure ; mRNA ; Peptides ; Poultry ; Pro-Opiomelanocortin - genetics ; Proprotein Convertase 2 - genetics ; Protein Precursors - genetics ; RNA, Messenger - metabolism ; Sequence analysis ; Thyroid ; Vertebrates ; Vertebrates: endocrinology</subject><ispartof>Endocrinology (Philadelphia), 2005-01, Vol.146 (1), p.301-308</ispartof><rights>Copyright © 2005 by The Endocrine Society 2005</rights><rights>2005 INIST-CNRS</rights><rights>Copyright © 2005 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-94c8f7552a72394ef927e0f551b7359fc74fd87f7c6a87fcf6a8241aba8c69b53</citedby><cites>FETCH-LOGICAL-c527t-94c8f7552a72394ef927e0f551b7359fc74fd87f7c6a87fcf6a8241aba8c69b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16444517$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15388646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vandenborne, Kristien</creatorcontrib><creatorcontrib>De Groef, Bert</creatorcontrib><creatorcontrib>Geelissen, Sofie M. E</creatorcontrib><creatorcontrib>Boorse, Graham C</creatorcontrib><creatorcontrib>Denver, Robert J</creatorcontrib><creatorcontrib>Kühn, Eduard R</creatorcontrib><creatorcontrib>Darras, Veerle M</creatorcontrib><creatorcontrib>Van der Geyten, Serge</creatorcontrib><title>Molecular Cloning and Developmental Expression of Corticotropin-Releasing Factor in the Chicken</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>We have characterized the structure of the chicken corticotropin-releasing factor (CRF) gene through cDNA cloning and genomic sequence analysis, and we analyzed the expression of CRF mRNA and peptide in the diencephalon of the chick throughout embryonic development. The structure of the chicken CRF gene is similar to other vertebrate CRF genes and contains two exons and a single intron. The primary structure of the mature chicken CRF peptide is identical to human and rat CRF. This is the first archosaurian CRF gene to be characterized. We used RIAs to analyze CRF peptide content in the diencephalon and the median eminence and plasma corticosterone during the last week of embryonic development. We also developed a semiquantitative RT-PCR method to analyze the expression of CRF mRNA during the same period. CRF peptide content in the diencephalon increased, whereas peptide content in the ME decreased just before hatching, suggesting that release and biosynthesis are coupled. Plasma corticosterone concentration significantly increased between embryonic d 20 and the first day post hatch. By contrast, CRF mRNA levels in the diencephalon decreased just before hatching. Changes in CRF production just before hatching may be causally related to the regulation of the thyroid and interrenal axes at this stage of chicken development.</description><subject>Aging - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Animals, Newborn - metabolism</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Chick Embryo - metabolism</subject><subject>Chick Embryo - physiology</subject><subject>Chickens</subject><subject>Chickens - blood</subject><subject>Chickens - genetics</subject><subject>Chickens - growth & development</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>Corticosterone</subject><subject>Corticosterone - blood</subject><subject>Corticotropin-releasing hormone</subject><subject>Corticotropin-Releasing Hormone - genetics</subject><subject>Corticotropin-Releasing Hormone - metabolism</subject><subject>Developmental stages</subject><subject>Diencephalon</subject><subject>Diencephalon - metabolism</subject><subject>Embryogenesis</subject><subject>Embryonic growth stage</subject><subject>Exons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genomic analysis</subject><subject>Hatching</subject><subject>Median Eminence - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Molecular Structure</subject><subject>mRNA</subject><subject>Peptides</subject><subject>Poultry</subject><subject>Pro-Opiomelanocortin - genetics</subject><subject>Proprotein Convertase 2 - genetics</subject><subject>Protein Precursors - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence analysis</subject><subject>Thyroid</subject><subject>Vertebrates</subject><subject>Vertebrates: endocrinology</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kd9rFDEQx4NY7LX65rMsiPXFrfmd3UdZWytUBNHnkMtNbGou2Sa7Uv97c72FA7FPXwY-M9-Z7yD0kuBzQgl-D_GcYsxbLHH3BK1Iz0WriMJP0QpjwlpFqTpGJ6Xc1pJzzp6hYyJY10kuV0h_SQHsHExuhpCijz8bEzfNR_gNIY1biJMJzcX9mKEUn2KTXDOkPHmbppxGH9tvEMCUXd-lsVPKjY_NdAPNcOPtL4jP0ZEzocCLRU_Rj8uL78NVe_310-fhw3VrBVVT23PbOSUENYqynoPrqQLshCBrxUTvrOJu0ymnrDRVrKtCOTFr01nZrwU7RWf7uWNOdzOUSW99sRCCiZDmoqVilLCeVPD1P-BtmnOsu2lGGBZSSaIq9W5P2ZxKyeD0mP3W5D-aYL2LXUPUu9j1LvaKv1qGzustbA7wknMF3iyAKdYEl020vhw4WR8jHnzf7rk0j49Ztosl25MQN8lmH-HhR4dr_rvoX4g_pyg</recordid><startdate>200501</startdate><enddate>200501</enddate><creator>Vandenborne, Kristien</creator><creator>De Groef, Bert</creator><creator>Geelissen, Sofie M. E</creator><creator>Boorse, Graham C</creator><creator>Denver, Robert J</creator><creator>Kühn, Eduard R</creator><creator>Darras, Veerle M</creator><creator>Van der Geyten, Serge</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200501</creationdate><title>Molecular Cloning and Developmental Expression of Corticotropin-Releasing Factor in the Chicken</title><author>Vandenborne, Kristien ; De Groef, Bert ; Geelissen, Sofie M. E ; Boorse, Graham C ; Denver, Robert J ; Kühn, Eduard R ; Darras, Veerle M ; Van der Geyten, Serge</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527t-94c8f7552a72394ef927e0f551b7359fc74fd87f7c6a87fcf6a8241aba8c69b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aging - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Animals, Newborn - metabolism</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Chick Embryo - metabolism</topic><topic>Chick Embryo - physiology</topic><topic>Chickens</topic><topic>Chickens - blood</topic><topic>Chickens - genetics</topic><topic>Chickens - growth & development</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>Corticosterone</topic><topic>Corticosterone - blood</topic><topic>Corticotropin-releasing hormone</topic><topic>Corticotropin-Releasing Hormone - genetics</topic><topic>Corticotropin-Releasing Hormone - metabolism</topic><topic>Developmental stages</topic><topic>Diencephalon</topic><topic>Diencephalon - metabolism</topic><topic>Embryogenesis</topic><topic>Embryonic growth stage</topic><topic>Exons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genomic analysis</topic><topic>Hatching</topic><topic>Median Eminence - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Molecular Structure</topic><topic>mRNA</topic><topic>Peptides</topic><topic>Poultry</topic><topic>Pro-Opiomelanocortin - genetics</topic><topic>Proprotein Convertase 2 - genetics</topic><topic>Protein Precursors - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence analysis</topic><topic>Thyroid</topic><topic>Vertebrates</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vandenborne, Kristien</creatorcontrib><creatorcontrib>De Groef, Bert</creatorcontrib><creatorcontrib>Geelissen, Sofie M. E</creatorcontrib><creatorcontrib>Boorse, Graham C</creatorcontrib><creatorcontrib>Denver, Robert J</creatorcontrib><creatorcontrib>Kühn, Eduard R</creatorcontrib><creatorcontrib>Darras, Veerle M</creatorcontrib><creatorcontrib>Van der Geyten, Serge</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vandenborne, Kristien</au><au>De Groef, Bert</au><au>Geelissen, Sofie M. E</au><au>Boorse, Graham C</au><au>Denver, Robert J</au><au>Kühn, Eduard R</au><au>Darras, Veerle M</au><au>Van der Geyten, Serge</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Cloning and Developmental Expression of Corticotropin-Releasing Factor in the Chicken</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>2005-01</date><risdate>2005</risdate><volume>146</volume><issue>1</issue><spage>301</spage><epage>308</epage><pages>301-308</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><coden>ENDOAO</coden><abstract>We have characterized the structure of the chicken corticotropin-releasing factor (CRF) gene through cDNA cloning and genomic sequence analysis, and we analyzed the expression of CRF mRNA and peptide in the diencephalon of the chick throughout embryonic development. The structure of the chicken CRF gene is similar to other vertebrate CRF genes and contains two exons and a single intron. The primary structure of the mature chicken CRF peptide is identical to human and rat CRF. This is the first archosaurian CRF gene to be characterized. We used RIAs to analyze CRF peptide content in the diencephalon and the median eminence and plasma corticosterone during the last week of embryonic development. We also developed a semiquantitative RT-PCR method to analyze the expression of CRF mRNA during the same period. CRF peptide content in the diencephalon increased, whereas peptide content in the ME decreased just before hatching, suggesting that release and biosynthesis are coupled. Plasma corticosterone concentration significantly increased between embryonic d 20 and the first day post hatch. By contrast, CRF mRNA levels in the diencephalon decreased just before hatching. Changes in CRF production just before hatching may be causally related to the regulation of the thyroid and interrenal axes at this stage of chicken development.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>15388646</pmid><doi>10.1210/en.2004-0608</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0013-7227 |
| ispartof | Endocrinology (Philadelphia), 2005-01, Vol.146 (1), p.301-308 |
| issn | 0013-7227 1945-7170 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67321391 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Aging - metabolism Amino Acid Sequence Animals Animals, Newborn - metabolism Base Sequence Biological and medical sciences Biosynthesis Chick Embryo - metabolism Chick Embryo - physiology Chickens Chickens - blood Chickens - genetics Chickens - growth & development Cloning Cloning, Molecular Corticosterone Corticosterone - blood Corticotropin-releasing hormone Corticotropin-Releasing Hormone - genetics Corticotropin-Releasing Hormone - metabolism Developmental stages Diencephalon Diencephalon - metabolism Embryogenesis Embryonic growth stage Exons Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Developmental Genomic analysis Hatching Median Eminence - metabolism Molecular Sequence Data Molecular Structure mRNA Peptides Poultry Pro-Opiomelanocortin - genetics Proprotein Convertase 2 - genetics Protein Precursors - genetics RNA, Messenger - metabolism Sequence analysis Thyroid Vertebrates Vertebrates: endocrinology |
| title | Molecular Cloning and Developmental Expression of Corticotropin-Releasing Factor in the Chicken |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T04%3A13%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20Cloning%20and%20Developmental%20Expression%20of%20Corticotropin-Releasing%20Factor%20in%20the%20Chicken&rft.jtitle=Endocrinology%20(Philadelphia)&rft.au=Vandenborne,%20Kristien&rft.date=2005-01&rft.volume=146&rft.issue=1&rft.spage=301&rft.epage=308&rft.pages=301-308&rft.issn=0013-7227&rft.eissn=1945-7170&rft.coden=ENDOAO&rft_id=info:doi/10.1210/en.2004-0608&rft_dat=%3Cproquest_cross%3E3130567617%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3130567617&rft_id=info:pmid/15388646&rft_oup_id=10.1210/en.2004-0608&rfr_iscdi=true |