Generation of porcine diploid blastocysts after injection of spermatozoa grown in nude mice
It is anticipated that the utilization of spermatogonia through testicular xenografting will open new avenues for the conservation of male gametes. With the aim of establishing this new technique for genetic preservation of pigs, we used it in combination with intracytoplasmic sperm injection (ICSI)...
Gespeichert in:
| Veröffentlicht in: | Theriogenology 2009-07, Vol.72 (1), p.2-9 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 9 |
|---|---|
| container_issue | 1 |
| container_start_page | 2 |
| container_title | Theriogenology |
| container_volume | 72 |
| creator | Nakai, M. Kaneko, H. Somfai, T. Maedomari, N. Ozawa, M. Noguchi, J. Kashiwazaki, N. Kikuchi, K. |
| description | It is anticipated that the utilization of spermatogonia through testicular xenografting will open new avenues for the conservation of male gametes. With the aim of establishing this new technique for genetic preservation of pigs, we used it in combination with intracytoplasmic sperm injection (ICSI). Testicular tissues derived from neonatal piglets, which contained seminiferous cords consisting of only gonocytes/spermatogonia, were transplanted under the back skin of castrated nude mice. Between 125 and 192 d after xenografting, sperm (morphologically similar to epididymal sperm) were recovered from 41 of the 65 host mice (63.1%). Testicular spermatozoa from adult boars were used as a positive control. A single spermatozoon was injected into an
in vitro matured porcine oocyte, and the oocytes were electro-stimulated and cultured (graft-ICSI and testis-ICSI, respectively). Blastocyst rates in both ICSI groups (24.9% and 37.4%, respectively) were higher (
P
<
0.05) than those without the injection procedure (parthenogenetic; 12.7%) and after injection of a small amount of injection buffer (sham; 13.0%). Rates of diploid blastocysts in both graft-ICSI and testis-ICSI groups (48.9% and 60.6%) were higher (
P
<
0.05) than those in the parthenogenetic and sham groups (13.5% and 28.0%). Therefore, we demonstrated that porcine oocytes injected with xenogeneic sperm have
in vitro developmental ability to the blastocyst stage. |
| doi_str_mv | 10.1016/j.theriogenology.2008.10.020 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67315645</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0093691X08007310</els_id><sourcerecordid>67315645</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-c30318e97ce49c4de7cacde65957267914631c8a81121112e99d568ff0c41d5a3</originalsourceid><addsrcrecordid>eNqNkE1r3DAQhkVJ6W7S_oVGh5CbtxrLli3IJYR8QaCHJhDoQWil8UaLLbmSt2H766tlt5Tcchjm8D7zwUPIGbAFMBDf1ovpBaMLK_ShD6vtomSszdGClewDmUPbyIKXHI7InDHJCyHheUaOU1ozxrgQ8InMQAKvywrm5Octeox6csHT0NExROM8UuvGPjhLl71OUzDbNCWquwkjdX6N5h-eRoyDnsKfoOkqhlefY-o3FungDH4mHzvdJ_xy6Cfk6eb68equePh-e391-VCYqqmmwnDGoUXZGKykqSw2RhuLopZ1U4pGQiU4mFa3ACXkQiltLdquY6YCW2t-Qs73e8cYfm0wTWpwyWDfa49hk5RoONSiqjN4sQdNDClF7NQY3aDjVgFTO7lqrd7KVTu5uzTLzeNfD3c2ywHt_-GDzQyc7oFOB6VX0SX19KNkwPNq3jawI272BGYfvx1GlYxDb9C6mLUqG9z7fvkLcYWeaw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67315645</pqid></control><display><type>article</type><title>Generation of porcine diploid blastocysts after injection of spermatozoa grown in nude mice</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Nakai, M. ; Kaneko, H. ; Somfai, T. ; Maedomari, N. ; Ozawa, M. ; Noguchi, J. ; Kashiwazaki, N. ; Kikuchi, K.</creator><creatorcontrib>Nakai, M. ; Kaneko, H. ; Somfai, T. ; Maedomari, N. ; Ozawa, M. ; Noguchi, J. ; Kashiwazaki, N. ; Kikuchi, K.</creatorcontrib><description>It is anticipated that the utilization of spermatogonia through testicular xenografting will open new avenues for the conservation of male gametes. With the aim of establishing this new technique for genetic preservation of pigs, we used it in combination with intracytoplasmic sperm injection (ICSI). Testicular tissues derived from neonatal piglets, which contained seminiferous cords consisting of only gonocytes/spermatogonia, were transplanted under the back skin of castrated nude mice. Between 125 and 192 d after xenografting, sperm (morphologically similar to epididymal sperm) were recovered from 41 of the 65 host mice (63.1%). Testicular spermatozoa from adult boars were used as a positive control. A single spermatozoon was injected into an
in vitro matured porcine oocyte, and the oocytes were electro-stimulated and cultured (graft-ICSI and testis-ICSI, respectively). Blastocyst rates in both ICSI groups (24.9% and 37.4%, respectively) were higher (
P
<
0.05) than those without the injection procedure (parthenogenetic; 12.7%) and after injection of a small amount of injection buffer (sham; 13.0%). Rates of diploid blastocysts in both graft-ICSI and testis-ICSI groups (48.9% and 60.6%) were higher (
P
<
0.05) than those in the parthenogenetic and sham groups (13.5% and 28.0%). Therefore, we demonstrated that porcine oocytes injected with xenogeneic sperm have
in vitro developmental ability to the blastocyst stage.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2008.10.020</identifier><identifier>PMID: 19135241</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Animals, Newborn ; assisted reproductive technologies ; blastocyst ; Blastocyst - physiology ; Diploidy ; embryo culture ; embryogenesis ; Embryonic Development ; Female ; ICSI ; injection ; intracytoplasmic sperm injection (ICSI) ; Karyotyping - veterinary ; Male ; Mice ; Mice, Nude ; oocytes ; Orchiectomy ; Pig ; Reproductive technology ; Sperm Injections, Intracytoplasmic - veterinary ; Spermatogenesis ; spermatozoa ; Spermatozoa - cytology ; Spermatozoa - growth & development ; Swine ; testes ; testicular xenografting ; Testis - cytology ; Testis - transplantation ; tissue transplantation ; Transplantation, Heterologous ; xenogeneic sperm ; Xenografting ; xenotransplantation</subject><ispartof>Theriogenology, 2009-07, Vol.72 (1), p.2-9</ispartof><rights>2009 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-c30318e97ce49c4de7cacde65957267914631c8a81121112e99d568ff0c41d5a3</citedby><cites>FETCH-LOGICAL-c474t-c30318e97ce49c4de7cacde65957267914631c8a81121112e99d568ff0c41d5a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0093691X08007310$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19135241$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakai, M.</creatorcontrib><creatorcontrib>Kaneko, H.</creatorcontrib><creatorcontrib>Somfai, T.</creatorcontrib><creatorcontrib>Maedomari, N.</creatorcontrib><creatorcontrib>Ozawa, M.</creatorcontrib><creatorcontrib>Noguchi, J.</creatorcontrib><creatorcontrib>Kashiwazaki, N.</creatorcontrib><creatorcontrib>Kikuchi, K.</creatorcontrib><title>Generation of porcine diploid blastocysts after injection of spermatozoa grown in nude mice</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>It is anticipated that the utilization of spermatogonia through testicular xenografting will open new avenues for the conservation of male gametes. With the aim of establishing this new technique for genetic preservation of pigs, we used it in combination with intracytoplasmic sperm injection (ICSI). Testicular tissues derived from neonatal piglets, which contained seminiferous cords consisting of only gonocytes/spermatogonia, were transplanted under the back skin of castrated nude mice. Between 125 and 192 d after xenografting, sperm (morphologically similar to epididymal sperm) were recovered from 41 of the 65 host mice (63.1%). Testicular spermatozoa from adult boars were used as a positive control. A single spermatozoon was injected into an
in vitro matured porcine oocyte, and the oocytes were electro-stimulated and cultured (graft-ICSI and testis-ICSI, respectively). Blastocyst rates in both ICSI groups (24.9% and 37.4%, respectively) were higher (
P
<
0.05) than those without the injection procedure (parthenogenetic; 12.7%) and after injection of a small amount of injection buffer (sham; 13.0%). Rates of diploid blastocysts in both graft-ICSI and testis-ICSI groups (48.9% and 60.6%) were higher (
P
<
0.05) than those in the parthenogenetic and sham groups (13.5% and 28.0%). Therefore, we demonstrated that porcine oocytes injected with xenogeneic sperm have
in vitro developmental ability to the blastocyst stage.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>assisted reproductive technologies</subject><subject>blastocyst</subject><subject>Blastocyst - physiology</subject><subject>Diploidy</subject><subject>embryo culture</subject><subject>embryogenesis</subject><subject>Embryonic Development</subject><subject>Female</subject><subject>ICSI</subject><subject>injection</subject><subject>intracytoplasmic sperm injection (ICSI)</subject><subject>Karyotyping - veterinary</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>oocytes</subject><subject>Orchiectomy</subject><subject>Pig</subject><subject>Reproductive technology</subject><subject>Sperm Injections, Intracytoplasmic - veterinary</subject><subject>Spermatogenesis</subject><subject>spermatozoa</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - growth & development</subject><subject>Swine</subject><subject>testes</subject><subject>testicular xenografting</subject><subject>Testis - cytology</subject><subject>Testis - transplantation</subject><subject>tissue transplantation</subject><subject>Transplantation, Heterologous</subject><subject>xenogeneic sperm</subject><subject>Xenografting</subject><subject>xenotransplantation</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1r3DAQhkVJ6W7S_oVGh5CbtxrLli3IJYR8QaCHJhDoQWil8UaLLbmSt2H766tlt5Tcchjm8D7zwUPIGbAFMBDf1ovpBaMLK_ShD6vtomSszdGClewDmUPbyIKXHI7InDHJCyHheUaOU1ozxrgQ8InMQAKvywrm5Octeox6csHT0NExROM8UuvGPjhLl71OUzDbNCWquwkjdX6N5h-eRoyDnsKfoOkqhlefY-o3FungDH4mHzvdJ_xy6Cfk6eb68equePh-e391-VCYqqmmwnDGoUXZGKykqSw2RhuLopZ1U4pGQiU4mFa3ACXkQiltLdquY6YCW2t-Qs73e8cYfm0wTWpwyWDfa49hk5RoONSiqjN4sQdNDClF7NQY3aDjVgFTO7lqrd7KVTu5uzTLzeNfD3c2ywHt_-GDzQyc7oFOB6VX0SX19KNkwPNq3jawI272BGYfvx1GlYxDb9C6mLUqG9z7fvkLcYWeaw</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>Nakai, M.</creator><creator>Kaneko, H.</creator><creator>Somfai, T.</creator><creator>Maedomari, N.</creator><creator>Ozawa, M.</creator><creator>Noguchi, J.</creator><creator>Kashiwazaki, N.</creator><creator>Kikuchi, K.</creator><general>Elsevier Inc</general><general>[Oxford]: Butterworth-Heinemann; [New York]: Elsevier Science</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090701</creationdate><title>Generation of porcine diploid blastocysts after injection of spermatozoa grown in nude mice</title><author>Nakai, M. ; Kaneko, H. ; Somfai, T. ; Maedomari, N. ; Ozawa, M. ; Noguchi, J. ; Kashiwazaki, N. ; Kikuchi, K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-c30318e97ce49c4de7cacde65957267914631c8a81121112e99d568ff0c41d5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>assisted reproductive technologies</topic><topic>blastocyst</topic><topic>Blastocyst - physiology</topic><topic>Diploidy</topic><topic>embryo culture</topic><topic>embryogenesis</topic><topic>Embryonic Development</topic><topic>Female</topic><topic>ICSI</topic><topic>injection</topic><topic>intracytoplasmic sperm injection (ICSI)</topic><topic>Karyotyping - veterinary</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>oocytes</topic><topic>Orchiectomy</topic><topic>Pig</topic><topic>Reproductive technology</topic><topic>Sperm Injections, Intracytoplasmic - veterinary</topic><topic>Spermatogenesis</topic><topic>spermatozoa</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - growth & development</topic><topic>Swine</topic><topic>testes</topic><topic>testicular xenografting</topic><topic>Testis - cytology</topic><topic>Testis - transplantation</topic><topic>tissue transplantation</topic><topic>Transplantation, Heterologous</topic><topic>xenogeneic sperm</topic><topic>Xenografting</topic><topic>xenotransplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakai, M.</creatorcontrib><creatorcontrib>Kaneko, H.</creatorcontrib><creatorcontrib>Somfai, T.</creatorcontrib><creatorcontrib>Maedomari, N.</creatorcontrib><creatorcontrib>Ozawa, M.</creatorcontrib><creatorcontrib>Noguchi, J.</creatorcontrib><creatorcontrib>Kashiwazaki, N.</creatorcontrib><creatorcontrib>Kikuchi, K.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakai, M.</au><au>Kaneko, H.</au><au>Somfai, T.</au><au>Maedomari, N.</au><au>Ozawa, M.</au><au>Noguchi, J.</au><au>Kashiwazaki, N.</au><au>Kikuchi, K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of porcine diploid blastocysts after injection of spermatozoa grown in nude mice</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2009-07-01</date><risdate>2009</risdate><volume>72</volume><issue>1</issue><spage>2</spage><epage>9</epage><pages>2-9</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>It is anticipated that the utilization of spermatogonia through testicular xenografting will open new avenues for the conservation of male gametes. With the aim of establishing this new technique for genetic preservation of pigs, we used it in combination with intracytoplasmic sperm injection (ICSI). Testicular tissues derived from neonatal piglets, which contained seminiferous cords consisting of only gonocytes/spermatogonia, were transplanted under the back skin of castrated nude mice. Between 125 and 192 d after xenografting, sperm (morphologically similar to epididymal sperm) were recovered from 41 of the 65 host mice (63.1%). Testicular spermatozoa from adult boars were used as a positive control. A single spermatozoon was injected into an
in vitro matured porcine oocyte, and the oocytes were electro-stimulated and cultured (graft-ICSI and testis-ICSI, respectively). Blastocyst rates in both ICSI groups (24.9% and 37.4%, respectively) were higher (
P
<
0.05) than those without the injection procedure (parthenogenetic; 12.7%) and after injection of a small amount of injection buffer (sham; 13.0%). Rates of diploid blastocysts in both graft-ICSI and testis-ICSI groups (48.9% and 60.6%) were higher (
P
<
0.05) than those in the parthenogenetic and sham groups (13.5% and 28.0%). Therefore, we demonstrated that porcine oocytes injected with xenogeneic sperm have
in vitro developmental ability to the blastocyst stage.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19135241</pmid><doi>10.1016/j.theriogenology.2008.10.020</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0093-691X |
| ispartof | Theriogenology, 2009-07, Vol.72 (1), p.2-9 |
| issn | 0093-691X 1879-3231 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67315645 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Animals, Newborn assisted reproductive technologies blastocyst Blastocyst - physiology Diploidy embryo culture embryogenesis Embryonic Development Female ICSI injection intracytoplasmic sperm injection (ICSI) Karyotyping - veterinary Male Mice Mice, Nude oocytes Orchiectomy Pig Reproductive technology Sperm Injections, Intracytoplasmic - veterinary Spermatogenesis spermatozoa Spermatozoa - cytology Spermatozoa - growth & development Swine testes testicular xenografting Testis - cytology Testis - transplantation tissue transplantation Transplantation, Heterologous xenogeneic sperm Xenografting xenotransplantation |
| title | Generation of porcine diploid blastocysts after injection of spermatozoa grown in nude mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T09%3A09%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Generation%20of%20porcine%20diploid%20blastocysts%20after%20injection%20of%20spermatozoa%20grown%20in%20nude%20mice&rft.jtitle=Theriogenology&rft.au=Nakai,%20M.&rft.date=2009-07-01&rft.volume=72&rft.issue=1&rft.spage=2&rft.epage=9&rft.pages=2-9&rft.issn=0093-691X&rft.eissn=1879-3231&rft_id=info:doi/10.1016/j.theriogenology.2008.10.020&rft_dat=%3Cproquest_cross%3E67315645%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67315645&rft_id=info:pmid/19135241&rft_els_id=S0093691X08007310&rfr_iscdi=true |