Simple method for screening of α-thalassaemia 1 carriers
α-Thalassaemia 1 genetic disorder occurs when there is a deletion of two linked α-globin genes. The interaction between these abnormal genes leads to the most severe type of thalassaemia disease, haemoglobin (Hb) Bart’s hydrops fetalis. The identification of α-thalassaemia 1 carriers and genetic cou...
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| Veröffentlicht in: | International journal of hematology 2009-06, Vol.89 (5), p.559-567 |
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| container_title | International journal of hematology |
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| creator | Tayapiwatana, Chatchai Kuntaruk, Surakit Tatu, Thanusak Chiampanichayakul, Sawitree Munkongdee, Thongperm Winichagoon, Pranee Fuchareon, Suthat Kasinrerk, Watchara |
| description | α-Thalassaemia 1 genetic disorder occurs when there is a deletion of two linked α-globin genes. The interaction between these abnormal genes leads to the most severe type of thalassaemia disease, haemoglobin (Hb) Bart’s hydrops fetalis. The identification of α-thalassaemia 1 carriers and genetic counselling are essential for the prevention and control of severe thalassaemia diseases. In this study, we have developed a rapid screening method for identifying α-thalassaemia 1. A sandwich-type immunochromatographic (IC) strip test was developed, using the generated monoclonal anti-Hb Bart’s antibody, to trace the Hb Bart’s in haemolysates. When assayed by our IC strip test, all α-thalassaemia 1, HbH disease, HbH-Constant Spring (H-CS) disease, HbH-CS and heterozygous HbE (CSEA) Bart’s disease, and homozygous α-thalassaemia 2 showed positive results. No false negative results were observed in these blood samples. In α-thalassaemia 2 heterozygotes, 83% of them showed positive reactivity. Among HbE (both homozygotes and heterozygotes), β-thalassaemia (heterozygotes, homozygotes and β-thalassaemia/HbE) and normal subjects, the IC strip test revealed negative reactivity of 100, 85 and 97%, respectively. These results indicate that this novel immunodiagnostic kit, in combination with red blood cell indices, is suitable for screening and ruling out mass populations for the presence of α-thalassaemia 1. |
| doi_str_mv | 10.1007/s12185-009-0331-4 |
| format | Article |
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The interaction between these abnormal genes leads to the most severe type of thalassaemia disease, haemoglobin (Hb) Bart’s hydrops fetalis. The identification of α-thalassaemia 1 carriers and genetic counselling are essential for the prevention and control of severe thalassaemia diseases. In this study, we have developed a rapid screening method for identifying α-thalassaemia 1. A sandwich-type immunochromatographic (IC) strip test was developed, using the generated monoclonal anti-Hb Bart’s antibody, to trace the Hb Bart’s in haemolysates. When assayed by our IC strip test, all α-thalassaemia 1, HbH disease, HbH-Constant Spring (H-CS) disease, HbH-CS and heterozygous HbE (CSEA) Bart’s disease, and homozygous α-thalassaemia 2 showed positive results. No false negative results were observed in these blood samples. In α-thalassaemia 2 heterozygotes, 83% of them showed positive reactivity. Among HbE (both homozygotes and heterozygotes), β-thalassaemia (heterozygotes, homozygotes and β-thalassaemia/HbE) and normal subjects, the IC strip test revealed negative reactivity of 100, 85 and 97%, respectively. These results indicate that this novel immunodiagnostic kit, in combination with red blood cell indices, is suitable for screening and ruling out mass populations for the presence of α-thalassaemia 1.</description><identifier>ISSN: 0925-5710</identifier><identifier>EISSN: 1865-3774</identifier><identifier>DOI: 10.1007/s12185-009-0331-4</identifier><identifier>PMID: 19440681</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>alpha-Thalassemia - diagnosis ; Anemias. Hemoglobinopathies ; Antibodies, Monoclonal ; Biological and medical sciences ; Chromatography ; Diseases of red blood cells ; Hematologic and hematopoietic diseases ; Hematology ; Hemoglobins, Abnormal - immunology ; Heterozygote ; Humans ; Immunoassay - methods ; Immunoassay - standards ; Mass Screening - methods ; Mass Screening - standards ; Medical sciences ; Medicine ; Medicine & Public Health ; Methods ; Oncology ; Original Article ; Reagent Kits, Diagnostic ; Sensitivity and Specificity</subject><ispartof>International journal of hematology, 2009-06, Vol.89 (5), p.559-567</ispartof><rights>The Japanese Society of Hematology 2009</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-44e11aaf2685767487c1809c76d7619e12eeb418f58b7e9e63f861af8b861c1d3</citedby><cites>FETCH-LOGICAL-c425t-44e11aaf2685767487c1809c76d7619e12eeb418f58b7e9e63f861af8b861c1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12185-009-0331-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12185-009-0331-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21672962$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19440681$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tayapiwatana, Chatchai</creatorcontrib><creatorcontrib>Kuntaruk, Surakit</creatorcontrib><creatorcontrib>Tatu, Thanusak</creatorcontrib><creatorcontrib>Chiampanichayakul, Sawitree</creatorcontrib><creatorcontrib>Munkongdee, Thongperm</creatorcontrib><creatorcontrib>Winichagoon, Pranee</creatorcontrib><creatorcontrib>Fuchareon, Suthat</creatorcontrib><creatorcontrib>Kasinrerk, Watchara</creatorcontrib><title>Simple method for screening of α-thalassaemia 1 carriers</title><title>International journal of hematology</title><addtitle>Int J Hematol</addtitle><addtitle>Int J Hematol</addtitle><description>α-Thalassaemia 1 genetic disorder occurs when there is a deletion of two linked α-globin genes. The interaction between these abnormal genes leads to the most severe type of thalassaemia disease, haemoglobin (Hb) Bart’s hydrops fetalis. The identification of α-thalassaemia 1 carriers and genetic counselling are essential for the prevention and control of severe thalassaemia diseases. In this study, we have developed a rapid screening method for identifying α-thalassaemia 1. A sandwich-type immunochromatographic (IC) strip test was developed, using the generated monoclonal anti-Hb Bart’s antibody, to trace the Hb Bart’s in haemolysates. When assayed by our IC strip test, all α-thalassaemia 1, HbH disease, HbH-Constant Spring (H-CS) disease, HbH-CS and heterozygous HbE (CSEA) Bart’s disease, and homozygous α-thalassaemia 2 showed positive results. No false negative results were observed in these blood samples. In α-thalassaemia 2 heterozygotes, 83% of them showed positive reactivity. Among HbE (both homozygotes and heterozygotes), β-thalassaemia (heterozygotes, homozygotes and β-thalassaemia/HbE) and normal subjects, the IC strip test revealed negative reactivity of 100, 85 and 97%, respectively. These results indicate that this novel immunodiagnostic kit, in combination with red blood cell indices, is suitable for screening and ruling out mass populations for the presence of α-thalassaemia 1.</description><subject>alpha-Thalassemia - diagnosis</subject><subject>Anemias. Hemoglobinopathies</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Chromatography</subject><subject>Diseases of red blood cells</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Hematology</subject><subject>Hemoglobins, Abnormal - immunology</subject><subject>Heterozygote</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Immunoassay - standards</subject><subject>Mass Screening - methods</subject><subject>Mass Screening - standards</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Methods</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Reagent Kits, Diagnostic</subject><subject>Sensitivity and Specificity</subject><issn>0925-5710</issn><issn>1865-3774</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL1OwzAUhS0EoqXwACwoC2wGX8exnREh_qRKDMBsue51myo_xW4GHosX4ZlwlQg2pjPc75wrfYScA7sGxtRNBA66oIyVlOU5UHFApqBlQXOlxCGZspIXtFDAJuQkxg1joJhQx2QCpRBMapiS8rVqtjVmDe7W3TLzXciiC4ht1a6yzmffX3S3trWN0WJT2QwyZ0OoMMRTcuRtHfFszBl5f7h_u3ui85fH57vbOXWCFzsqBAJY67nUhZJKaOVAs9IpuVQSSgSOuBCgfaEXCkuUudcSrNeLFA6W-YxcDbvb0H30GHemqaLDurYtdn00UuWslFwnEAbQhS7GgN5sQ9XY8GmAmb0vM_gyyZfZ-zIidS7G8X7R4PKvMQpKwOUI2Ohs7YNtXRV_OQ5S8fQ9cXzgYjq1Kwxm0_WhTWL--f4DNEWBdA</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Tayapiwatana, Chatchai</creator><creator>Kuntaruk, Surakit</creator><creator>Tatu, Thanusak</creator><creator>Chiampanichayakul, Sawitree</creator><creator>Munkongdee, Thongperm</creator><creator>Winichagoon, Pranee</creator><creator>Fuchareon, Suthat</creator><creator>Kasinrerk, Watchara</creator><general>Springer Japan</general><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>Simple method for screening of α-thalassaemia 1 carriers</title><author>Tayapiwatana, Chatchai ; Kuntaruk, Surakit ; Tatu, Thanusak ; Chiampanichayakul, Sawitree ; Munkongdee, Thongperm ; Winichagoon, Pranee ; Fuchareon, Suthat ; Kasinrerk, Watchara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-44e11aaf2685767487c1809c76d7619e12eeb418f58b7e9e63f861af8b861c1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>alpha-Thalassemia - diagnosis</topic><topic>Anemias. Hemoglobinopathies</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Chromatography</topic><topic>Diseases of red blood cells</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Hematology</topic><topic>Hemoglobins, Abnormal - immunology</topic><topic>Heterozygote</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Immunoassay - standards</topic><topic>Mass Screening - methods</topic><topic>Mass Screening - standards</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Methods</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Reagent Kits, Diagnostic</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tayapiwatana, Chatchai</creatorcontrib><creatorcontrib>Kuntaruk, Surakit</creatorcontrib><creatorcontrib>Tatu, Thanusak</creatorcontrib><creatorcontrib>Chiampanichayakul, Sawitree</creatorcontrib><creatorcontrib>Munkongdee, Thongperm</creatorcontrib><creatorcontrib>Winichagoon, Pranee</creatorcontrib><creatorcontrib>Fuchareon, Suthat</creatorcontrib><creatorcontrib>Kasinrerk, Watchara</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of hematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tayapiwatana, Chatchai</au><au>Kuntaruk, Surakit</au><au>Tatu, Thanusak</au><au>Chiampanichayakul, Sawitree</au><au>Munkongdee, Thongperm</au><au>Winichagoon, Pranee</au><au>Fuchareon, Suthat</au><au>Kasinrerk, Watchara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simple method for screening of α-thalassaemia 1 carriers</atitle><jtitle>International journal of hematology</jtitle><stitle>Int J Hematol</stitle><addtitle>Int J Hematol</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>89</volume><issue>5</issue><spage>559</spage><epage>567</epage><pages>559-567</pages><issn>0925-5710</issn><eissn>1865-3774</eissn><abstract>α-Thalassaemia 1 genetic disorder occurs when there is a deletion of two linked α-globin genes. The interaction between these abnormal genes leads to the most severe type of thalassaemia disease, haemoglobin (Hb) Bart’s hydrops fetalis. The identification of α-thalassaemia 1 carriers and genetic counselling are essential for the prevention and control of severe thalassaemia diseases. In this study, we have developed a rapid screening method for identifying α-thalassaemia 1. A sandwich-type immunochromatographic (IC) strip test was developed, using the generated monoclonal anti-Hb Bart’s antibody, to trace the Hb Bart’s in haemolysates. When assayed by our IC strip test, all α-thalassaemia 1, HbH disease, HbH-Constant Spring (H-CS) disease, HbH-CS and heterozygous HbE (CSEA) Bart’s disease, and homozygous α-thalassaemia 2 showed positive results. No false negative results were observed in these blood samples. In α-thalassaemia 2 heterozygotes, 83% of them showed positive reactivity. Among HbE (both homozygotes and heterozygotes), β-thalassaemia (heterozygotes, homozygotes and β-thalassaemia/HbE) and normal subjects, the IC strip test revealed negative reactivity of 100, 85 and 97%, respectively. These results indicate that this novel immunodiagnostic kit, in combination with red blood cell indices, is suitable for screening and ruling out mass populations for the presence of α-thalassaemia 1.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>19440681</pmid><doi>10.1007/s12185-009-0331-4</doi><tpages>9</tpages></addata></record> |
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| ispartof | International journal of hematology, 2009-06, Vol.89 (5), p.559-567 |
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| subjects | alpha-Thalassemia - diagnosis Anemias. Hemoglobinopathies Antibodies, Monoclonal Biological and medical sciences Chromatography Diseases of red blood cells Hematologic and hematopoietic diseases Hematology Hemoglobins, Abnormal - immunology Heterozygote Humans Immunoassay - methods Immunoassay - standards Mass Screening - methods Mass Screening - standards Medical sciences Medicine Medicine & Public Health Methods Oncology Original Article Reagent Kits, Diagnostic Sensitivity and Specificity |
| title | Simple method for screening of α-thalassaemia 1 carriers |
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