cell-based system for screening hair growth-promoting agents
Androgen-inducible transforming growth factor β (TGF-β1) derived from dermal papilla cells (DPCs) is a catagen inducer that mediates hair growth suppression in androgenetic alopecia (AGA). In this study, a cell-based assay system was developed to monitor TGF-β1 promoter activity and then used to eva...
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| Veröffentlicht in: | Archives of Dermatological Research 2009-06, Vol.301 (5), p.381-385 |
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| creator | Huh, Sungran Lee, Jongsung Jung, Eunsun Kim, Sang-Cheol Kang, Jung-Il Lee, Jienny Kim, Yong-Woo Sung, Young Kwan Kang, Hee-Kyoung Park, Deokhoon |
| description | Androgen-inducible transforming growth factor β (TGF-β1) derived from dermal papilla cells (DPCs) is a catagen inducer that mediates hair growth suppression in androgenetic alopecia (AGA). In this study, a cell-based assay system was developed to monitor TGF-β1 promoter activity and then used to evaluate the effects of activated TGF-β1 promoter in human epidermal keratinocytes (HaCaT). To accomplish this, a pMetLuc-TGF-β1 promoter plasmid that expresses the luciferase reporter gene in response to TGF-β1 promoter activity was constructed. Treatment of HaCaT with dihydrotestosterone, which is known to be a primary factor of AGA, resulted in a concentration-dependent increase in TGF-β1 promoter activity. However, treatment of HaCaT with the TGF-β1 inhibitor, curcumin, resulted in a concentration-dependant decrease in TGF-β1 expression. Subsequent use of this assay system to screen TGF-β1 revealed that HaCaT that were treated with apigenin showed decreased levels of TGF-β1 expression. In addition, treatment with apigenin also significantly increased the proliferation of both SV40T-DPCs (human DPCs) and HaCaT cells. Furthermore, apigenin stimulated the elongation of hair follicles in a rat vibrissa hair follicle organ culture. Taken together, these findings suggest that apigenin, which is known to have antioxidant, anti-inflammatory, and anti-tumor properties, stimulates hair growth through downregulation of the TGF-β1 gene. In addition, these results suggest that this assay system could be used to quantitatively measure TGF-β1 promoter activity in HaCaT, thereby facilitating the screening of agents promoting hair growth. |
| doi_str_mv | 10.1007/s00403-009-0931-0 |
| format | Article |
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In this study, a cell-based assay system was developed to monitor TGF-β1 promoter activity and then used to evaluate the effects of activated TGF-β1 promoter in human epidermal keratinocytes (HaCaT). To accomplish this, a pMetLuc-TGF-β1 promoter plasmid that expresses the luciferase reporter gene in response to TGF-β1 promoter activity was constructed. Treatment of HaCaT with dihydrotestosterone, which is known to be a primary factor of AGA, resulted in a concentration-dependent increase in TGF-β1 promoter activity. However, treatment of HaCaT with the TGF-β1 inhibitor, curcumin, resulted in a concentration-dependant decrease in TGF-β1 expression. Subsequent use of this assay system to screen TGF-β1 revealed that HaCaT that were treated with apigenin showed decreased levels of TGF-β1 expression. In addition, treatment with apigenin also significantly increased the proliferation of both SV40T-DPCs (human DPCs) and HaCaT cells. Furthermore, apigenin stimulated the elongation of hair follicles in a rat vibrissa hair follicle organ culture. Taken together, these findings suggest that apigenin, which is known to have antioxidant, anti-inflammatory, and anti-tumor properties, stimulates hair growth through downregulation of the TGF-β1 gene. In addition, these results suggest that this assay system could be used to quantitatively measure TGF-β1 promoter activity in HaCaT, thereby facilitating the screening of agents promoting hair growth.</description><identifier>ISSN: 0340-3696</identifier><identifier>EISSN: 1432-069X</identifier><identifier>DOI: 10.1007/s00403-009-0931-0</identifier><identifier>PMID: 19277688</identifier><identifier>CODEN: ADREDL</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Alopecia - immunology ; Alopecia - pathology ; Alopecia - physiopathology ; Alopecia - therapy ; Animals ; Apigenin - pharmacology ; Biological and medical sciences ; Cell Culture Techniques ; Cell Growth Processes - drug effects ; Cell Growth Processes - immunology ; Curcumin - pharmacology ; Dermatology ; Drug Evaluation, Preclinical - methods ; Epidermis - pathology ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - immunology ; Hair - drug effects ; Hair - growth & development ; Hair - immunology ; Hair - metabolism ; Humans ; Immunotherapy ; Keratinocytes - drug effects ; Keratinocytes - immunology ; Keratinocytes - metabolism ; Keratinocytes - pathology ; Medical sciences ; Medicine ; Medicine & Public Health ; Promoter Regions, Genetic ; Rats ; Short Communication ; Transcriptional Activation - drug effects ; Transcriptional Activation - immunology ; Transforming Growth Factor beta1 - genetics ; Transforming Growth Factor beta1 - immunology ; Transforming Growth Factor beta1 - metabolism ; Vibrissae - drug effects ; Vibrissae - immunology ; Vibrissae - metabolism ; Vibrissae - pathology</subject><ispartof>Archives of Dermatological Research, 2009-06, Vol.301 (5), p.381-385</ispartof><rights>Springer-Verlag 2009</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-8572fdf6efce313265065fff652cdcd3fe0bc0f62121e1b63c9364acbfcb7a2c3</citedby><cites>FETCH-LOGICAL-c423t-8572fdf6efce313265065fff652cdcd3fe0bc0f62121e1b63c9364acbfcb7a2c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00403-009-0931-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00403-009-0931-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21556965$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19277688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huh, Sungran</creatorcontrib><creatorcontrib>Lee, Jongsung</creatorcontrib><creatorcontrib>Jung, Eunsun</creatorcontrib><creatorcontrib>Kim, Sang-Cheol</creatorcontrib><creatorcontrib>Kang, Jung-Il</creatorcontrib><creatorcontrib>Lee, Jienny</creatorcontrib><creatorcontrib>Kim, Yong-Woo</creatorcontrib><creatorcontrib>Sung, Young Kwan</creatorcontrib><creatorcontrib>Kang, Hee-Kyoung</creatorcontrib><creatorcontrib>Park, Deokhoon</creatorcontrib><title>cell-based system for screening hair growth-promoting agents</title><title>Archives of Dermatological Research</title><addtitle>Arch Dermatol Res</addtitle><addtitle>Arch Dermatol Res</addtitle><description>Androgen-inducible transforming growth factor β (TGF-β1) derived from dermal papilla cells (DPCs) is a catagen inducer that mediates hair growth suppression in androgenetic alopecia (AGA). In this study, a cell-based assay system was developed to monitor TGF-β1 promoter activity and then used to evaluate the effects of activated TGF-β1 promoter in human epidermal keratinocytes (HaCaT). To accomplish this, a pMetLuc-TGF-β1 promoter plasmid that expresses the luciferase reporter gene in response to TGF-β1 promoter activity was constructed. Treatment of HaCaT with dihydrotestosterone, which is known to be a primary factor of AGA, resulted in a concentration-dependent increase in TGF-β1 promoter activity. However, treatment of HaCaT with the TGF-β1 inhibitor, curcumin, resulted in a concentration-dependant decrease in TGF-β1 expression. Subsequent use of this assay system to screen TGF-β1 revealed that HaCaT that were treated with apigenin showed decreased levels of TGF-β1 expression. In addition, treatment with apigenin also significantly increased the proliferation of both SV40T-DPCs (human DPCs) and HaCaT cells. Furthermore, apigenin stimulated the elongation of hair follicles in a rat vibrissa hair follicle organ culture. Taken together, these findings suggest that apigenin, which is known to have antioxidant, anti-inflammatory, and anti-tumor properties, stimulates hair growth through downregulation of the TGF-β1 gene. In addition, these results suggest that this assay system could be used to quantitatively measure TGF-β1 promoter activity in HaCaT, thereby facilitating the screening of agents promoting hair growth.</description><subject>Alopecia - immunology</subject><subject>Alopecia - pathology</subject><subject>Alopecia - physiopathology</subject><subject>Alopecia - therapy</subject><subject>Animals</subject><subject>Apigenin - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Culture Techniques</subject><subject>Cell Growth Processes - drug effects</subject><subject>Cell Growth Processes - immunology</subject><subject>Curcumin - pharmacology</subject><subject>Dermatology</subject><subject>Drug Evaluation, Preclinical - methods</subject><subject>Epidermis - pathology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene Expression Regulation - immunology</subject><subject>Hair - drug effects</subject><subject>Hair - growth & development</subject><subject>Hair - immunology</subject><subject>Hair - metabolism</subject><subject>Humans</subject><subject>Immunotherapy</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - immunology</subject><subject>Keratinocytes - metabolism</subject><subject>Keratinocytes - pathology</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Promoter Regions, Genetic</subject><subject>Rats</subject><subject>Short Communication</subject><subject>Transcriptional Activation - drug effects</subject><subject>Transcriptional Activation - immunology</subject><subject>Transforming Growth Factor beta1 - genetics</subject><subject>Transforming Growth Factor beta1 - immunology</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><subject>Vibrissae - drug effects</subject><subject>Vibrissae - immunology</subject><subject>Vibrissae - metabolism</subject><subject>Vibrissae - pathology</subject><issn>0340-3696</issn><issn>1432-069X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUFL5TAUhYOM6OPpD3CjRXB20ZvcNnkFN4M4oyC4UMFdSNOkVl5bze1j8N-b0ofCLCabC8l3Tg7nMnYk4FwA6AsCyAE5QMmhRMFhhy1EjpKDKp9_sAVgDhxVqfbZIdErpKMhl6D32L4opdZqtVqwS-fXa15Z8nVGHzT6LgtDzMhF7_u2b7IX28asicPf8YW_xaEbxunWNr4f6YDtBrsmf7idS_b0-_rx6obf3f-5vfp1x10uceSrQstQB-WD8yhQqgJUEUJQhXS1qzF4qBwEJYUUXlQKXYkqt64KrtJWOlyyn7NvCvC-8TSarqUpuO39sCGjNKYaUCfw9B_wddjEPmUzk_lK5AITJGbIxYEo-mDeYtvZ-GEEmKlaM1drkqeZqjWQNMdb403V-fpbsS0yAWdbwJKz6xBt71r64qQoirSKInFy5ig99Y2P3wn_9_vJLAp2MLaJyfjpQYJAEAqV1hI_AfWhmVg</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Huh, Sungran</creator><creator>Lee, Jongsung</creator><creator>Jung, Eunsun</creator><creator>Kim, Sang-Cheol</creator><creator>Kang, Jung-Il</creator><creator>Lee, Jienny</creator><creator>Kim, Yong-Woo</creator><creator>Sung, Young Kwan</creator><creator>Kang, Hee-Kyoung</creator><creator>Park, Deokhoon</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>cell-based system for screening hair growth-promoting agents</title><author>Huh, Sungran ; Lee, Jongsung ; Jung, Eunsun ; Kim, Sang-Cheol ; Kang, Jung-Il ; Lee, Jienny ; Kim, Yong-Woo ; Sung, Young Kwan ; Kang, Hee-Kyoung ; Park, Deokhoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-8572fdf6efce313265065fff652cdcd3fe0bc0f62121e1b63c9364acbfcb7a2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Alopecia - 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drug effects</topic><topic>Transcriptional Activation - immunology</topic><topic>Transforming Growth Factor beta1 - genetics</topic><topic>Transforming Growth Factor beta1 - immunology</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><topic>Vibrissae - drug effects</topic><topic>Vibrissae - immunology</topic><topic>Vibrissae - metabolism</topic><topic>Vibrissae - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huh, Sungran</creatorcontrib><creatorcontrib>Lee, Jongsung</creatorcontrib><creatorcontrib>Jung, Eunsun</creatorcontrib><creatorcontrib>Kim, Sang-Cheol</creatorcontrib><creatorcontrib>Kang, Jung-Il</creatorcontrib><creatorcontrib>Lee, Jienny</creatorcontrib><creatorcontrib>Kim, Yong-Woo</creatorcontrib><creatorcontrib>Sung, Young Kwan</creatorcontrib><creatorcontrib>Kang, Hee-Kyoung</creatorcontrib><creatorcontrib>Park, Deokhoon</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of Dermatological Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huh, Sungran</au><au>Lee, Jongsung</au><au>Jung, Eunsun</au><au>Kim, Sang-Cheol</au><au>Kang, Jung-Il</au><au>Lee, Jienny</au><au>Kim, Yong-Woo</au><au>Sung, Young Kwan</au><au>Kang, Hee-Kyoung</au><au>Park, Deokhoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cell-based system for screening hair growth-promoting agents</atitle><jtitle>Archives of Dermatological Research</jtitle><stitle>Arch Dermatol Res</stitle><addtitle>Arch Dermatol Res</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>301</volume><issue>5</issue><spage>381</spage><epage>385</epage><pages>381-385</pages><issn>0340-3696</issn><eissn>1432-069X</eissn><coden>ADREDL</coden><abstract>Androgen-inducible transforming growth factor β (TGF-β1) derived from dermal papilla cells (DPCs) is a catagen inducer that mediates hair growth suppression in androgenetic alopecia (AGA). In this study, a cell-based assay system was developed to monitor TGF-β1 promoter activity and then used to evaluate the effects of activated TGF-β1 promoter in human epidermal keratinocytes (HaCaT). To accomplish this, a pMetLuc-TGF-β1 promoter plasmid that expresses the luciferase reporter gene in response to TGF-β1 promoter activity was constructed. Treatment of HaCaT with dihydrotestosterone, which is known to be a primary factor of AGA, resulted in a concentration-dependent increase in TGF-β1 promoter activity. However, treatment of HaCaT with the TGF-β1 inhibitor, curcumin, resulted in a concentration-dependant decrease in TGF-β1 expression. Subsequent use of this assay system to screen TGF-β1 revealed that HaCaT that were treated with apigenin showed decreased levels of TGF-β1 expression. In addition, treatment with apigenin also significantly increased the proliferation of both SV40T-DPCs (human DPCs) and HaCaT cells. Furthermore, apigenin stimulated the elongation of hair follicles in a rat vibrissa hair follicle organ culture. Taken together, these findings suggest that apigenin, which is known to have antioxidant, anti-inflammatory, and anti-tumor properties, stimulates hair growth through downregulation of the TGF-β1 gene. In addition, these results suggest that this assay system could be used to quantitatively measure TGF-β1 promoter activity in HaCaT, thereby facilitating the screening of agents promoting hair growth.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19277688</pmid><doi>10.1007/s00403-009-0931-0</doi><tpages>5</tpages></addata></record> |
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| ispartof | Archives of Dermatological Research, 2009-06, Vol.301 (5), p.381-385 |
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| subjects | Alopecia - immunology Alopecia - pathology Alopecia - physiopathology Alopecia - therapy Animals Apigenin - pharmacology Biological and medical sciences Cell Culture Techniques Cell Growth Processes - drug effects Cell Growth Processes - immunology Curcumin - pharmacology Dermatology Drug Evaluation, Preclinical - methods Epidermis - pathology Gene Expression Regulation - drug effects Gene Expression Regulation - immunology Hair - drug effects Hair - growth & development Hair - immunology Hair - metabolism Humans Immunotherapy Keratinocytes - drug effects Keratinocytes - immunology Keratinocytes - metabolism Keratinocytes - pathology Medical sciences Medicine Medicine & Public Health Promoter Regions, Genetic Rats Short Communication Transcriptional Activation - drug effects Transcriptional Activation - immunology Transforming Growth Factor beta1 - genetics Transforming Growth Factor beta1 - immunology Transforming Growth Factor beta1 - metabolism Vibrissae - drug effects Vibrissae - immunology Vibrissae - metabolism Vibrissae - pathology |
| title | cell-based system for screening hair growth-promoting agents |
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