Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC–ELSD
A high-performance liquid chromatographic method with evaporative light scattering detection (HPLC–ELSD) has been developed to identify and quantify 19 ginsenosides (Rg 1, Re, Rf, Rb 1, Rc, Rb 2, Rd, F 4, Rg 6, Rk 3, Rh 4, 20( S)-, 20( R)-Rg 3, 20( S)-, 20( R)-Rs 3, Rk 1, Rg 5, Rs 4, and Rs 5) in bl...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2009-08, Vol.50 (1), p.15-22 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Sun, Bai-Shen Gu, Li-Juan Fang, Zhe-Ming Wang, Chun-yan Wang, Zhen Lee, Mi-Ra Li, Zheng Li, Jing-Jie Sung, Chang-Keun |
| description | A high-performance liquid chromatographic method with evaporative light scattering detection (HPLC–ELSD) has been developed to identify and quantify 19 ginsenosides (Rg
1, Re, Rf, Rb
1, Rc, Rb
2, Rd, F
4, Rg
6, Rk
3, Rh
4, 20(
S)-, 20(
R)-Rg
3, 20(
S)-, 20(
R)-Rs
3, Rk
1, Rg
5, Rs
4, and Rs
5) in black ginseng (BG, Korean white ginseng that was subjected to nine cycles of steam treatment). Ultrasonication is employed for sample preparation, and the analysis is achieved on a Discovery C
18 column using gradient elution of CH
3CN–H
2O–CH
3COOH without buffer in 40
min. The method was validated by linearity (
r
2
≥
0.9994), precision (92.0–107.5%), intra- and inter-day accuracy (R.S.D.
<
3.21%), and limit of detection (LOD
≤
93
ng). The quantification method was applied to analyze the composition of ginsenosides in Korean white, red, and black ginsengs. During the preparatory process of BG, ginsenosides transform into constituents of low polarity by hydrolysis, isomerization, and dehydration at C-20, and hydrolysis also occurs at C-3 or C-6. The validated HPLC method is expected to provide the basis for the quality assessment of ginseng products. |
| doi_str_mv | 10.1016/j.jpba.2009.03.025 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67286339</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S073170850900199X</els_id><sourcerecordid>67286339</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-dc42fcbb729a57d4c84877a43d3af308e2df677ffb85e683ac909e8138e8e9d63</originalsourceid><addsrcrecordid>eNqFkc1u1DAUhS1ERYfCC7BA3sAuqX8S_0hs0NBSpJGoVJDYWY59XXman6mdVO2u79A35EnIaKKyg5Wl6-8c3XsOQu8oKSmh4nRbbneNLRkhuiS8JKx-gVZUSV4wUf16iVZEclpIoupj9DrnLSGkprp6hY6p5rqSSqzQzVXspna0PQxTxreT7ccYorNjHHo8BEw1vo59hn7I0UPGscdNa93NMr3GHu6gHXbgcUhDhy9tb--fP5sHfHG5Wf9-fDrbXH15g46CbTO8Xd4T9PP87Mf6oth8__pt_XlTuIrWY-FdxYJrGsm0raWvnKqUlLbintvAiQLmg5AyhEbVIBS3ThMNinIFCrQX_AR9PPju0nA7QR5NF7ODtj1caYRkSnCu_wvOyQrFdD2D7AC6NOScIJhdip1ND4YSs-_CbM2-i71CG8LN3MUser-4T00H_q9kCX8GPiyAzc62IdnexfzMMVorrcTe6NOBgzm0uwjJZBehd-BjAjcaP8R_7fEHIk2pKA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20068295</pqid></control><display><type>article</type><title>Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC–ELSD</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Sun, Bai-Shen ; Gu, Li-Juan ; Fang, Zhe-Ming ; Wang, Chun-yan ; Wang, Zhen ; Lee, Mi-Ra ; Li, Zheng ; Li, Jing-Jie ; Sung, Chang-Keun</creator><creatorcontrib>Sun, Bai-Shen ; Gu, Li-Juan ; Fang, Zhe-Ming ; Wang, Chun-yan ; Wang, Zhen ; Lee, Mi-Ra ; Li, Zheng ; Li, Jing-Jie ; Sung, Chang-Keun</creatorcontrib><description>A high-performance liquid chromatographic method with evaporative light scattering detection (HPLC–ELSD) has been developed to identify and quantify 19 ginsenosides (Rg
1, Re, Rf, Rb
1, Rc, Rb
2, Rd, F
4, Rg
6, Rk
3, Rh
4, 20(
S)-, 20(
R)-Rg
3, 20(
S)-, 20(
R)-Rs
3, Rk
1, Rg
5, Rs
4, and Rs
5) in black ginseng (BG, Korean white ginseng that was subjected to nine cycles of steam treatment). Ultrasonication is employed for sample preparation, and the analysis is achieved on a Discovery C
18 column using gradient elution of CH
3CN–H
2O–CH
3COOH without buffer in 40
min. The method was validated by linearity (
r
2
≥
0.9994), precision (92.0–107.5%), intra- and inter-day accuracy (R.S.D.
<
3.21%), and limit of detection (LOD
≤
93
ng). The quantification method was applied to analyze the composition of ginsenosides in Korean white, red, and black ginsengs. During the preparatory process of BG, ginsenosides transform into constituents of low polarity by hydrolysis, isomerization, and dehydration at C-20, and hydrolysis also occurs at C-3 or C-6. The validated HPLC method is expected to provide the basis for the quality assessment of ginseng products.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2009.03.025</identifier><identifier>PMID: 19394786</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Black ginseng ; Chromatography, High Pressure Liquid - methods ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Ginsenoside ; Ginsenosides - analysis ; HPLC–ELSD ; Medical sciences ; Panax - chemistry ; Panax ginseng ; Pharmacology. Drug treatments ; Quantification ; Reproducibility of Results ; Steaming</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2009-08, Vol.50 (1), p.15-22</ispartof><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-dc42fcbb729a57d4c84877a43d3af308e2df677ffb85e683ac909e8138e8e9d63</citedby><cites>FETCH-LOGICAL-c415t-dc42fcbb729a57d4c84877a43d3af308e2df677ffb85e683ac909e8138e8e9d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2009.03.025$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21589865$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19394786$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Bai-Shen</creatorcontrib><creatorcontrib>Gu, Li-Juan</creatorcontrib><creatorcontrib>Fang, Zhe-Ming</creatorcontrib><creatorcontrib>Wang, Chun-yan</creatorcontrib><creatorcontrib>Wang, Zhen</creatorcontrib><creatorcontrib>Lee, Mi-Ra</creatorcontrib><creatorcontrib>Li, Zheng</creatorcontrib><creatorcontrib>Li, Jing-Jie</creatorcontrib><creatorcontrib>Sung, Chang-Keun</creatorcontrib><title>Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC–ELSD</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A high-performance liquid chromatographic method with evaporative light scattering detection (HPLC–ELSD) has been developed to identify and quantify 19 ginsenosides (Rg
1, Re, Rf, Rb
1, Rc, Rb
2, Rd, F
4, Rg
6, Rk
3, Rh
4, 20(
S)-, 20(
R)-Rg
3, 20(
S)-, 20(
R)-Rs
3, Rk
1, Rg
5, Rs
4, and Rs
5) in black ginseng (BG, Korean white ginseng that was subjected to nine cycles of steam treatment). Ultrasonication is employed for sample preparation, and the analysis is achieved on a Discovery C
18 column using gradient elution of CH
3CN–H
2O–CH
3COOH without buffer in 40
min. The method was validated by linearity (
r
2
≥
0.9994), precision (92.0–107.5%), intra- and inter-day accuracy (R.S.D.
<
3.21%), and limit of detection (LOD
≤
93
ng). The quantification method was applied to analyze the composition of ginsenosides in Korean white, red, and black ginsengs. During the preparatory process of BG, ginsenosides transform into constituents of low polarity by hydrolysis, isomerization, and dehydration at C-20, and hydrolysis also occurs at C-3 or C-6. The validated HPLC method is expected to provide the basis for the quality assessment of ginseng products.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Black ginseng</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Ginsenoside</subject><subject>Ginsenosides - analysis</subject><subject>HPLC–ELSD</subject><subject>Medical sciences</subject><subject>Panax - chemistry</subject><subject>Panax ginseng</subject><subject>Pharmacology. Drug treatments</subject><subject>Quantification</subject><subject>Reproducibility of Results</subject><subject>Steaming</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS1ERYfCC7BA3sAuqX8S_0hs0NBSpJGoVJDYWY59XXman6mdVO2u79A35EnIaKKyg5Wl6-8c3XsOQu8oKSmh4nRbbneNLRkhuiS8JKx-gVZUSV4wUf16iVZEclpIoupj9DrnLSGkprp6hY6p5rqSSqzQzVXspna0PQxTxreT7ccYorNjHHo8BEw1vo59hn7I0UPGscdNa93NMr3GHu6gHXbgcUhDhy9tb--fP5sHfHG5Wf9-fDrbXH15g46CbTO8Xd4T9PP87Mf6oth8__pt_XlTuIrWY-FdxYJrGsm0raWvnKqUlLbintvAiQLmg5AyhEbVIBS3ThMNinIFCrQX_AR9PPju0nA7QR5NF7ODtj1caYRkSnCu_wvOyQrFdD2D7AC6NOScIJhdip1ND4YSs-_CbM2-i71CG8LN3MUser-4T00H_q9kCX8GPiyAzc62IdnexfzMMVorrcTe6NOBgzm0uwjJZBehd-BjAjcaP8R_7fEHIk2pKA</recordid><startdate>20090815</startdate><enddate>20090815</enddate><creator>Sun, Bai-Shen</creator><creator>Gu, Li-Juan</creator><creator>Fang, Zhe-Ming</creator><creator>Wang, Chun-yan</creator><creator>Wang, Zhen</creator><creator>Lee, Mi-Ra</creator><creator>Li, Zheng</creator><creator>Li, Jing-Jie</creator><creator>Sung, Chang-Keun</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090815</creationdate><title>Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC–ELSD</title><author>Sun, Bai-Shen ; Gu, Li-Juan ; Fang, Zhe-Ming ; Wang, Chun-yan ; Wang, Zhen ; Lee, Mi-Ra ; Li, Zheng ; Li, Jing-Jie ; Sung, Chang-Keun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-dc42fcbb729a57d4c84877a43d3af308e2df677ffb85e683ac909e8138e8e9d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Black ginseng</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Ginsenoside</topic><topic>Ginsenosides - analysis</topic><topic>HPLC–ELSD</topic><topic>Medical sciences</topic><topic>Panax - chemistry</topic><topic>Panax ginseng</topic><topic>Pharmacology. Drug treatments</topic><topic>Quantification</topic><topic>Reproducibility of Results</topic><topic>Steaming</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Bai-Shen</creatorcontrib><creatorcontrib>Gu, Li-Juan</creatorcontrib><creatorcontrib>Fang, Zhe-Ming</creatorcontrib><creatorcontrib>Wang, Chun-yan</creatorcontrib><creatorcontrib>Wang, Zhen</creatorcontrib><creatorcontrib>Lee, Mi-Ra</creatorcontrib><creatorcontrib>Li, Zheng</creatorcontrib><creatorcontrib>Li, Jing-Jie</creatorcontrib><creatorcontrib>Sung, Chang-Keun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Bai-Shen</au><au>Gu, Li-Juan</au><au>Fang, Zhe-Ming</au><au>Wang, Chun-yan</au><au>Wang, Zhen</au><au>Lee, Mi-Ra</au><au>Li, Zheng</au><au>Li, Jing-Jie</au><au>Sung, Chang-Keun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC–ELSD</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2009-08-15</date><risdate>2009</risdate><volume>50</volume><issue>1</issue><spage>15</spage><epage>22</epage><pages>15-22</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A high-performance liquid chromatographic method with evaporative light scattering detection (HPLC–ELSD) has been developed to identify and quantify 19 ginsenosides (Rg
1, Re, Rf, Rb
1, Rc, Rb
2, Rd, F
4, Rg
6, Rk
3, Rh
4, 20(
S)-, 20(
R)-Rg
3, 20(
S)-, 20(
R)-Rs
3, Rk
1, Rg
5, Rs
4, and Rs
5) in black ginseng (BG, Korean white ginseng that was subjected to nine cycles of steam treatment). Ultrasonication is employed for sample preparation, and the analysis is achieved on a Discovery C
18 column using gradient elution of CH
3CN–H
2O–CH
3COOH without buffer in 40
min. The method was validated by linearity (
r
2
≥
0.9994), precision (92.0–107.5%), intra- and inter-day accuracy (R.S.D.
<
3.21%), and limit of detection (LOD
≤
93
ng). The quantification method was applied to analyze the composition of ginsenosides in Korean white, red, and black ginsengs. During the preparatory process of BG, ginsenosides transform into constituents of low polarity by hydrolysis, isomerization, and dehydration at C-20, and hydrolysis also occurs at C-3 or C-6. The validated HPLC method is expected to provide the basis for the quality assessment of ginseng products.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19394786</pmid><doi>10.1016/j.jpba.2009.03.025</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2009-08, Vol.50 (1), p.15-22 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67286339 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Black ginseng Chromatography, High Pressure Liquid - methods Fundamental and applied biological sciences. Psychology General pharmacology Ginsenoside Ginsenosides - analysis HPLC–ELSD Medical sciences Panax - chemistry Panax ginseng Pharmacology. Drug treatments Quantification Reproducibility of Results Steaming |
| title | Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC–ELSD |
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