Simultaneous quantitation of tobramycin and colistin sulphate by HPLC with evaporative light scattering detection
A rapid and simple method for the simultaneous determination of tobramycin and colistin sulphate in a pharmaceutical formulation by reversed phase HPLC and evaporative light scattering detection is described. Chromatographic separation was carried out in gradient mode using a Zorbax SB C18 column (1...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2009-08, Vol.50 (1), p.64-67 |
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| container_issue | 1 |
| container_start_page | 64 |
| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Clarot, I. Storme-Paris, I. Chaminade, P. Estevenon, O. Nicolas, A. Rieutord, A. |
| description | A rapid and simple method for the simultaneous determination of tobramycin and colistin sulphate in a pharmaceutical formulation by reversed phase HPLC and evaporative light scattering detection is described. Chromatographic separation was carried out in gradient mode using a Zorbax SB C18 column (150
mm
×
4
mm, 3.5
μm) with mobile phases of acetonitrile and water containing trifluoroacetic at 1
ml/min. The method was validated using methodology described by the International Conference of Harmonization. The method was shown to be specific, precise, accurate and linear. Real samples were analyzed to demonstrate the applicability of the chromatographic method in a routine use. |
| doi_str_mv | 10.1016/j.jpba.2009.03.013 |
| format | Article |
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mm
×
4
mm, 3.5
μm) with mobile phases of acetonitrile and water containing trifluoroacetic at 1
ml/min. The method was validated using methodology described by the International Conference of Harmonization. The method was shown to be specific, precise, accurate and linear. Real samples were analyzed to demonstrate the applicability of the chromatographic method in a routine use.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2009.03.013</identifier><identifier>PMID: 19372021</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Colistin - analysis ; Colistin sulphate ; ELSD ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; HPLC ; Light ; Medical sciences ; Pharmaceutical formulation ; Pharmacology. Drug treatments ; Reference Standards ; Reproducibility of Results ; Scattering, Radiation ; Sensitivity and Specificity ; Tobramycin ; Tobramycin - analysis</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2009-08, Vol.50 (1), p.64-67</ispartof><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-84f6f66b81dca694e3542b9c537697f9d37157a59d6c564930ffafc8a9027e253</citedby><cites>FETCH-LOGICAL-c440t-84f6f66b81dca694e3542b9c537697f9d37157a59d6c564930ffafc8a9027e253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0731708509001903$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21589873$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19372021$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clarot, I.</creatorcontrib><creatorcontrib>Storme-Paris, I.</creatorcontrib><creatorcontrib>Chaminade, P.</creatorcontrib><creatorcontrib>Estevenon, O.</creatorcontrib><creatorcontrib>Nicolas, A.</creatorcontrib><creatorcontrib>Rieutord, A.</creatorcontrib><title>Simultaneous quantitation of tobramycin and colistin sulphate by HPLC with evaporative light scattering detection</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A rapid and simple method for the simultaneous determination of tobramycin and colistin sulphate in a pharmaceutical formulation by reversed phase HPLC and evaporative light scattering detection is described. Chromatographic separation was carried out in gradient mode using a Zorbax SB C18 column (150
mm
×
4
mm, 3.5
μm) with mobile phases of acetonitrile and water containing trifluoroacetic at 1
ml/min. The method was validated using methodology described by the International Conference of Harmonization. The method was shown to be specific, precise, accurate and linear. Real samples were analyzed to demonstrate the applicability of the chromatographic method in a routine use.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Colistin - analysis</subject><subject>Colistin sulphate</subject><subject>ELSD</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>HPLC</subject><subject>Light</subject><subject>Medical sciences</subject><subject>Pharmaceutical formulation</subject><subject>Pharmacology. Drug treatments</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Scattering, Radiation</subject><subject>Sensitivity and Specificity</subject><subject>Tobramycin</subject><subject>Tobramycin - analysis</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2L1DAAhoO4uLOrf8CD5KK3dvPRJA14kUHdhQEFFbyFNE12MrRNJ0lH5t-bYQb35p5C4HlfkvcB4C1GNUaY3-3q3dzpmiAka0RrhOkLsMKtoBXhze-XYIUExZVALbsGNyntEEIMy-YVuMaSCoIIXoH9Dz8uQ9aTDUuC-0VP2WedfZhgcDCHLurxaPwE9dRDEwafcrmkZZi3OlvYHeH9980a_vF5C-1BzyGW8MHCwT9uM0xG52yjnx5hb7M1p97X4MrpIdk3l_MW_Pry-ef6vtp8-_qw_rSpTNOgXLWN447zrsW90Vw2lrKGdNIwKrgUTvZUYCY0kz03jDeSIue0M62WiAhLGL0FH869cwz7xaasRp-MHYbzXxUXpCUMPw-WfUVh2wKSM2hiSClap-boRx2PCiN1MqJ26mTklJAKUVWMlNC7S_vSjbZ_ilwUFOD9BdBlrsFFPRmf_nEEs1YWp4X7eOZsGe3gbVTJeDsZ2_tYllV98P97x18Sk6tI</recordid><startdate>20090815</startdate><enddate>20090815</enddate><creator>Clarot, I.</creator><creator>Storme-Paris, I.</creator><creator>Chaminade, P.</creator><creator>Estevenon, O.</creator><creator>Nicolas, A.</creator><creator>Rieutord, A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090815</creationdate><title>Simultaneous quantitation of tobramycin and colistin sulphate by HPLC with evaporative light scattering detection</title><author>Clarot, I. ; Storme-Paris, I. ; Chaminade, P. ; Estevenon, O. ; Nicolas, A. ; Rieutord, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-84f6f66b81dca694e3542b9c537697f9d37157a59d6c564930ffafc8a9027e253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Colistin - analysis</topic><topic>Colistin sulphate</topic><topic>ELSD</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>HPLC</topic><topic>Light</topic><topic>Medical sciences</topic><topic>Pharmaceutical formulation</topic><topic>Pharmacology. Drug treatments</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Scattering, Radiation</topic><topic>Sensitivity and Specificity</topic><topic>Tobramycin</topic><topic>Tobramycin - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clarot, I.</creatorcontrib><creatorcontrib>Storme-Paris, I.</creatorcontrib><creatorcontrib>Chaminade, P.</creatorcontrib><creatorcontrib>Estevenon, O.</creatorcontrib><creatorcontrib>Nicolas, A.</creatorcontrib><creatorcontrib>Rieutord, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clarot, I.</au><au>Storme-Paris, I.</au><au>Chaminade, P.</au><au>Estevenon, O.</au><au>Nicolas, A.</au><au>Rieutord, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous quantitation of tobramycin and colistin sulphate by HPLC with evaporative light scattering detection</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2009-08-15</date><risdate>2009</risdate><volume>50</volume><issue>1</issue><spage>64</spage><epage>67</epage><pages>64-67</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A rapid and simple method for the simultaneous determination of tobramycin and colistin sulphate in a pharmaceutical formulation by reversed phase HPLC and evaporative light scattering detection is described. 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mm
×
4
mm, 3.5
μm) with mobile phases of acetonitrile and water containing trifluoroacetic at 1
ml/min. The method was validated using methodology described by the International Conference of Harmonization. The method was shown to be specific, precise, accurate and linear. Real samples were analyzed to demonstrate the applicability of the chromatographic method in a routine use.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19372021</pmid><doi>10.1016/j.jpba.2009.03.013</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2009-08, Vol.50 (1), p.64-67 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Colistin - analysis Colistin sulphate ELSD Fundamental and applied biological sciences. Psychology General pharmacology HPLC Light Medical sciences Pharmaceutical formulation Pharmacology. Drug treatments Reference Standards Reproducibility of Results Scattering, Radiation Sensitivity and Specificity Tobramycin Tobramycin - analysis |
| title | Simultaneous quantitation of tobramycin and colistin sulphate by HPLC with evaporative light scattering detection |
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