Evidence for Na+/Ca2+ Exchanger 1 Association with Caveolin‐1 and ‐2 in C6 Glioma Cells
The purpose of this study is to understand the interaction of Na+‐Ca2+ exchanger (NCX1), that is one of the essential regulators of Ca2+ homeostasis, with caveolin (Cav)‐1 and Cav‐2 in Cav‐3 null cell (rat C6 glioma cell). Both mRNA and protein expression of NCX1, Cav‐1 and Cav‐2 was observed, but n...
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| creator | Cha, Seok Ho Shin, Sun Young Jung, Sang Yong Kim, Yun Tai Park, Yong Joo Kwak, Jin‐Oh Kim, Hyun‐Woo Suh, Chang Kook |
| description | The purpose of this study is to understand the interaction of Na+‐Ca2+ exchanger (NCX1), that is one of the essential regulators of Ca2+ homeostasis, with caveolin (Cav)‐1 and Cav‐2 in Cav‐3 null cell (rat C6 glioma cell). Both mRNA and protein expression of NCX1, Cav‐1 and Cav‐2 was observed, but no expression of mRNA and protein of Cav‐3 were observed in C6 glioma cells. In isolated caveolae‐enriched membrane fraction, the NCX1, Cav‐1 and Cav‐2 proteins localized in same fractions. The experiment of immuno‐precipitation showed complex formation between the NCX1 and Cavs. Confocal microscopy also supported co‐localization of NCX1 and Cavs at the plasma membrane. Functionally, sodium‐free induced forward mode of NCX1 attenuated by Cav‐1 antisense ODN. When treated cells with Cav‐2 antisense ODN, both reverse and forward mode of NCX1 was attenuated. From these results, in the Cav‐3 lacking cells, the function of NCX1 might be regulated by binding with Cavs. Considering the decrement of NCX1 activity by antisense ODNs, caveolins may play an important role in diverse of pathophysiological process of NCX1‐related disorders in the body.
IUBMB Life, 56: 621‐627, 2004 |
| doi_str_mv | 10.1080/15216540400020353 |
| format | Article |
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IUBMB Life, 56: 621‐627, 2004</description><identifier>ISSN: 1521-6543</identifier><identifier>EISSN: 1521-6551</identifier><identifier>DOI: 10.1080/15216540400020353</identifier><identifier>PMID: 15814461</identifier><language>eng</language><publisher>UK: Informa Healthcare</publisher><subject>Animals ; Binding Sites ; Blotting, Western ; C6 glioma cells ; Calcium - metabolism ; Caveolae ; Caveolin (Cav) ; Caveolin 1 ; Caveolin 2 ; Caveolins - metabolism ; Cell Line, Tumor ; Cell Membrane - metabolism ; Humans ; Immunoprecipitation ; Immuno‐precipitaion (IP) ; Ions ; Microscopy, Confocal ; Na+‐Ca2+ exchanger (NCX) ; Oligonucleotides, Antisense - pharmacology ; Protein Binding ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Sodium - pharmacology ; Sodium-Calcium Exchanger - metabolism ; Time Factors</subject><ispartof>IUBMB life, 2004-10, Vol.56 (10), p.621-627</ispartof><rights>Copyright © 2004 International Union of Biochemistry and Molecular Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3258-31f99355f1df6b52e4625fac83ae08a7637438ada120a17a8a555d35585f45b33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1080%2F15216540400020353$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1080%2F15216540400020353$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,1432,27922,27923,45572,45573,46407,46831</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15814461$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cha, Seok Ho</creatorcontrib><creatorcontrib>Shin, Sun Young</creatorcontrib><creatorcontrib>Jung, Sang Yong</creatorcontrib><creatorcontrib>Kim, Yun Tai</creatorcontrib><creatorcontrib>Park, Yong Joo</creatorcontrib><creatorcontrib>Kwak, Jin‐Oh</creatorcontrib><creatorcontrib>Kim, Hyun‐Woo</creatorcontrib><creatorcontrib>Suh, Chang Kook</creatorcontrib><title>Evidence for Na+/Ca2+ Exchanger 1 Association with Caveolin‐1 and ‐2 in C6 Glioma Cells</title><title>IUBMB life</title><addtitle>IUBMB Life</addtitle><description>The purpose of this study is to understand the interaction of Na+‐Ca2+ exchanger (NCX1), that is one of the essential regulators of Ca2+ homeostasis, with caveolin (Cav)‐1 and Cav‐2 in Cav‐3 null cell (rat C6 glioma cell). Both mRNA and protein expression of NCX1, Cav‐1 and Cav‐2 was observed, but no expression of mRNA and protein of Cav‐3 were observed in C6 glioma cells. In isolated caveolae‐enriched membrane fraction, the NCX1, Cav‐1 and Cav‐2 proteins localized in same fractions. The experiment of immuno‐precipitation showed complex formation between the NCX1 and Cavs. Confocal microscopy also supported co‐localization of NCX1 and Cavs at the plasma membrane. Functionally, sodium‐free induced forward mode of NCX1 attenuated by Cav‐1 antisense ODN. When treated cells with Cav‐2 antisense ODN, both reverse and forward mode of NCX1 was attenuated. From these results, in the Cav‐3 lacking cells, the function of NCX1 might be regulated by binding with Cavs. Considering the decrement of NCX1 activity by antisense ODNs, caveolins may play an important role in diverse of pathophysiological process of NCX1‐related disorders in the body.
IUBMB Life, 56: 621‐627, 2004</description><subject>Animals</subject><subject>Binding Sites</subject><subject>Blotting, Western</subject><subject>C6 glioma cells</subject><subject>Calcium - metabolism</subject><subject>Caveolae</subject><subject>Caveolin (Cav)</subject><subject>Caveolin 1</subject><subject>Caveolin 2</subject><subject>Caveolins - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Membrane - metabolism</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>Immuno‐precipitaion (IP)</subject><subject>Ions</subject><subject>Microscopy, Confocal</subject><subject>Na+‐Ca2+ exchanger (NCX)</subject><subject>Oligonucleotides, Antisense - pharmacology</subject><subject>Protein Binding</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Sodium - pharmacology</subject><subject>Sodium-Calcium Exchanger - metabolism</subject><subject>Time Factors</subject><issn>1521-6543</issn><issn>1521-6551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1Ow0AQhVcIRELgADRoK5rIZGf_vJFoEisEpABNqCisib0mixw7ePNDOo7AGTkJjhJBQUE1b0bfexo9Qs6BXQEzrAOKg1aSScYYZ0KJA9Lc3gKtFBz-aCka5MT715piIesekwYoA1JqaJLnwcqltkgszcqKPmC7EyFv08F7MsXixVYUaM_7MnG4cGVB124xpRGubJm74uvjEygWKa0Fp66gkabD3JUzpJHNc39KjjLMvT3bzxZ5uhmMo9tg9Di8i3qjIBFcmUBA1u0KpTJIMz1R3ErNVYaJEWiZwVCLUAqDKQJnCCEaVEqltcGoTKqJEC1yucudV-Xb0vpFPHM-qT_AwpZLH-uQh0bLbg3CDkyq0vvKZvG8cjOsNjGweNto_KfR2nOxD19OZjb9dewrrIHrHbB2ud38nxiP-_f9EETI68WIb3F4fus</recordid><startdate>200410</startdate><enddate>200410</enddate><creator>Cha, Seok Ho</creator><creator>Shin, Sun Young</creator><creator>Jung, Sang Yong</creator><creator>Kim, Yun Tai</creator><creator>Park, Yong Joo</creator><creator>Kwak, Jin‐Oh</creator><creator>Kim, Hyun‐Woo</creator><creator>Suh, Chang Kook</creator><general>Informa Healthcare</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200410</creationdate><title>Evidence for Na+/Ca2+ Exchanger 1 Association with Caveolin‐1 and ‐2 in C6 Glioma Cells</title><author>Cha, Seok Ho ; Shin, Sun Young ; Jung, Sang Yong ; Kim, Yun Tai ; Park, Yong Joo ; Kwak, Jin‐Oh ; Kim, Hyun‐Woo ; Suh, Chang Kook</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3258-31f99355f1df6b52e4625fac83ae08a7637438ada120a17a8a555d35585f45b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Binding Sites</topic><topic>Blotting, Western</topic><topic>C6 glioma cells</topic><topic>Calcium - metabolism</topic><topic>Caveolae</topic><topic>Caveolin (Cav)</topic><topic>Caveolin 1</topic><topic>Caveolin 2</topic><topic>Caveolins - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Membrane - metabolism</topic><topic>Humans</topic><topic>Immunoprecipitation</topic><topic>Immuno‐precipitaion (IP)</topic><topic>Ions</topic><topic>Microscopy, Confocal</topic><topic>Na+‐Ca2+ exchanger (NCX)</topic><topic>Oligonucleotides, Antisense - pharmacology</topic><topic>Protein Binding</topic><topic>Rats</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Sodium - pharmacology</topic><topic>Sodium-Calcium Exchanger - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cha, Seok Ho</creatorcontrib><creatorcontrib>Shin, Sun Young</creatorcontrib><creatorcontrib>Jung, Sang Yong</creatorcontrib><creatorcontrib>Kim, Yun Tai</creatorcontrib><creatorcontrib>Park, Yong Joo</creatorcontrib><creatorcontrib>Kwak, Jin‐Oh</creatorcontrib><creatorcontrib>Kim, Hyun‐Woo</creatorcontrib><creatorcontrib>Suh, Chang Kook</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>IUBMB life</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cha, Seok Ho</au><au>Shin, Sun Young</au><au>Jung, Sang Yong</au><au>Kim, Yun Tai</au><au>Park, Yong Joo</au><au>Kwak, Jin‐Oh</au><au>Kim, Hyun‐Woo</au><au>Suh, Chang Kook</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for Na+/Ca2+ Exchanger 1 Association with Caveolin‐1 and ‐2 in C6 Glioma Cells</atitle><jtitle>IUBMB life</jtitle><addtitle>IUBMB Life</addtitle><date>2004-10</date><risdate>2004</risdate><volume>56</volume><issue>10</issue><spage>621</spage><epage>627</epage><pages>621-627</pages><issn>1521-6543</issn><eissn>1521-6551</eissn><abstract>The purpose of this study is to understand the interaction of Na+‐Ca2+ exchanger (NCX1), that is one of the essential regulators of Ca2+ homeostasis, with caveolin (Cav)‐1 and Cav‐2 in Cav‐3 null cell (rat C6 glioma cell). Both mRNA and protein expression of NCX1, Cav‐1 and Cav‐2 was observed, but no expression of mRNA and protein of Cav‐3 were observed in C6 glioma cells. In isolated caveolae‐enriched membrane fraction, the NCX1, Cav‐1 and Cav‐2 proteins localized in same fractions. The experiment of immuno‐precipitation showed complex formation between the NCX1 and Cavs. Confocal microscopy also supported co‐localization of NCX1 and Cavs at the plasma membrane. Functionally, sodium‐free induced forward mode of NCX1 attenuated by Cav‐1 antisense ODN. When treated cells with Cav‐2 antisense ODN, both reverse and forward mode of NCX1 was attenuated. From these results, in the Cav‐3 lacking cells, the function of NCX1 might be regulated by binding with Cavs. Considering the decrement of NCX1 activity by antisense ODNs, caveolins may play an important role in diverse of pathophysiological process of NCX1‐related disorders in the body.
IUBMB Life, 56: 621‐627, 2004</abstract><cop>UK</cop><pub>Informa Healthcare</pub><pmid>15814461</pmid><doi>10.1080/15216540400020353</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Binding Sites Blotting, Western C6 glioma cells Calcium - metabolism Caveolae Caveolin (Cav) Caveolin 1 Caveolin 2 Caveolins - metabolism Cell Line, Tumor Cell Membrane - metabolism Humans Immunoprecipitation Immuno‐precipitaion (IP) Ions Microscopy, Confocal Na+‐Ca2+ exchanger (NCX) Oligonucleotides, Antisense - pharmacology Protein Binding Rats Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sodium - pharmacology Sodium-Calcium Exchanger - metabolism Time Factors |
| title | Evidence for Na+/Ca2+ Exchanger 1 Association with Caveolin‐1 and ‐2 in C6 Glioma Cells |
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