The histological characteristics of cultured oral epithelium in different culture conditions
This study was undertaken to establish the characterization of cultured oral mucosal epithelium and introducing them as an alternative source for reconstruction of ocular surface disease. Human oral epithelial cells were cultured on simple media (DMEM/HF12) as control and co-cultured on mitomycin C-...
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| Veröffentlicht in: | Iranian biomedical journal 2009-04, Vol.13 (2), p.109-115 |
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| creator | Hashemi, Hassan Salehnia, Mojdeh Kamali, Morvarid Beigi Boroujeni, Mandana |
| description | This study was undertaken to establish the characterization of cultured oral mucosal epithelium and introducing them as an alternative source for reconstruction of ocular surface disease.
Human oral epithelial cells were cultured on simple media (DMEM/HF12) as control and co-cultured on mitomycin C-treated 3T3 feeder layer, on the amniotic membrane (AM) without nitrocellulose and the mitotically inactivated 3T3 fibroblast, and on the sandwich layer of AM fastened on the nitrocellulose as insert and 3T3 fibroblast. After 3 weeks, the characteristics of the cells were assessed morphologically and also ultrastructurally using scanning electron microscopy and transmission electron microscopy and immuno-cytochemically.
The epithelial cells were cultured on AM spread on nitrocellulose insert and 3T3 feeder layer showed better growth than other groups and all groups of study were shown similar characteristics. The cultured oral epithelial shared the characteristics with corneal epithelium.
Thus the oral epithelial could be an alternative source for transplantation. |
| format | Article |
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Human oral epithelial cells were cultured on simple media (DMEM/HF12) as control and co-cultured on mitomycin C-treated 3T3 feeder layer, on the amniotic membrane (AM) without nitrocellulose and the mitotically inactivated 3T3 fibroblast, and on the sandwich layer of AM fastened on the nitrocellulose as insert and 3T3 fibroblast. After 3 weeks, the characteristics of the cells were assessed morphologically and also ultrastructurally using scanning electron microscopy and transmission electron microscopy and immuno-cytochemically.
The epithelial cells were cultured on AM spread on nitrocellulose insert and 3T3 feeder layer showed better growth than other groups and all groups of study were shown similar characteristics. The cultured oral epithelial shared the characteristics with corneal epithelium.
Thus the oral epithelial could be an alternative source for transplantation.</description><identifier>ISSN: 1028-852X</identifier><identifier>EISSN: 2008-823X</identifier><identifier>PMID: 19471551</identifier><language>eng</language><publisher>Iran: Pasteur Institute of Iran</publisher><subject>3T3 Cells ; Animals ; Cell Culture Techniques ; Cell Proliferation ; Cell Shape ; Cells, Cultured ; Epithelial Cells - cytology ; Epithelial Cells - ultrastructure ; Humans ; Immunohistochemistry ; Keratin-12 - metabolism ; Keratin-3 - metabolism ; Mice ; Mouth Mucosa - cytology ; Mouth Mucosa - ultrastructure</subject><ispartof>Iranian biomedical journal, 2009-04, Vol.13 (2), p.109-115</ispartof><rights>Copyright Dr Ali Akbari Sari, Director of The Commission for Accreditation & Improvement of Iranian Medical Journals Apr 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19471551$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hashemi, Hassan</creatorcontrib><creatorcontrib>Salehnia, Mojdeh</creatorcontrib><creatorcontrib>Kamali, Morvarid</creatorcontrib><creatorcontrib>Beigi Boroujeni, Mandana</creatorcontrib><title>The histological characteristics of cultured oral epithelium in different culture conditions</title><title>Iranian biomedical journal</title><addtitle>Iran Biomed J</addtitle><description>This study was undertaken to establish the characterization of cultured oral mucosal epithelium and introducing them as an alternative source for reconstruction of ocular surface disease.
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The epithelial cells were cultured on AM spread on nitrocellulose insert and 3T3 feeder layer showed better growth than other groups and all groups of study were shown similar characteristics. The cultured oral epithelial shared the characteristics with corneal epithelium.
Thus the oral epithelial could be an alternative source for transplantation.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Cell Culture Techniques</subject><subject>Cell Proliferation</subject><subject>Cell Shape</subject><subject>Cells, Cultured</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - ultrastructure</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Keratin-12 - metabolism</subject><subject>Keratin-3 - metabolism</subject><subject>Mice</subject><subject>Mouth Mucosa - cytology</subject><subject>Mouth Mucosa - ultrastructure</subject><issn>1028-852X</issn><issn>2008-823X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpd0MtKxDAUBuAgijOOvoIEF-4KuTRNupTBGwy4GWEWQkmTU5uhbWouC9_eguPG1TkcPn4O_xlaM0JUoRg_nKM1JWzZBTus0FWMR0K4oFJeohWtS0mFoGv0se8B9y4mP_hPZ_SATa-DNgnCcnQmYt9hk4eUA1jswwJgdqmHweURuwlb13UQYEp_Chs_WZecn-I1uuj0EOHmNDfo_elxv30pdm_Pr9uHXTFTQVNRlq2pK6mI5HXNwdi6pQKstgK4qigXQulaqFJUBIjRhErSmorZVnIOrVR8g-5_c-fgvzLE1IwuGhgGPYHPsakkk5JRvsC7f_Doc5iW3xomhVpqI-WCbk8otyPYZg5u1OG7-SuN_wC75Wo3</recordid><startdate>200904</startdate><enddate>200904</enddate><creator>Hashemi, Hassan</creator><creator>Salehnia, Mojdeh</creator><creator>Kamali, Morvarid</creator><creator>Beigi Boroujeni, Mandana</creator><general>Pasteur Institute of Iran</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QO</scope><scope>7T5</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>200904</creationdate><title>The histological characteristics of cultured oral epithelium in different culture conditions</title><author>Hashemi, Hassan ; 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Human oral epithelial cells were cultured on simple media (DMEM/HF12) as control and co-cultured on mitomycin C-treated 3T3 feeder layer, on the amniotic membrane (AM) without nitrocellulose and the mitotically inactivated 3T3 fibroblast, and on the sandwich layer of AM fastened on the nitrocellulose as insert and 3T3 fibroblast. After 3 weeks, the characteristics of the cells were assessed morphologically and also ultrastructurally using scanning electron microscopy and transmission electron microscopy and immuno-cytochemically.
The epithelial cells were cultured on AM spread on nitrocellulose insert and 3T3 feeder layer showed better growth than other groups and all groups of study were shown similar characteristics. The cultured oral epithelial shared the characteristics with corneal epithelium.
Thus the oral epithelial could be an alternative source for transplantation.</abstract><cop>Iran</cop><pub>Pasteur Institute of Iran</pub><pmid>19471551</pmid><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 1028-852X |
| ispartof | Iranian biomedical journal, 2009-04, Vol.13 (2), p.109-115 |
| issn | 1028-852X 2008-823X |
| language | eng |
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| source | MEDLINE; Directory of Open Journals (DOAJ); EZB-FREE-00999 freely available EZB journals |
| subjects | 3T3 Cells Animals Cell Culture Techniques Cell Proliferation Cell Shape Cells, Cultured Epithelial Cells - cytology Epithelial Cells - ultrastructure Humans Immunohistochemistry Keratin-12 - metabolism Keratin-3 - metabolism Mice Mouth Mucosa - cytology Mouth Mucosa - ultrastructure |
| title | The histological characteristics of cultured oral epithelium in different culture conditions |
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