Supported Bilayers Formed from Different Phospholipids on Spherical Silica Substrates
Spherical supported bilayer membranes (SS-BLMs) are very attractive candidates in modern bioanalytics and biorecognition studies. A uniform, facile method of preparing different SS-BLMs on silica beads is reported. Confocal fluorescence microscopy and cryo-TEM imaging have been used to characterize...
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| Veröffentlicht in: | Langmuir 2009-05, Vol.25 (10), p.5455-5458 |
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| creator | Gopalakrishnan, Gopakumar Rouiller, Isabelle Colman, David R Lennox, R. Bruce |
| description | Spherical supported bilayer membranes (SS-BLMs) are very attractive candidates in modern bioanalytics and biorecognition studies. A uniform, facile method of preparing different SS-BLMs on silica beads is reported. Confocal fluorescence microscopy and cryo-TEM imaging have been used to characterize these SS-BLMs. Thermal analysis data and FRAP experiments show that the bilayer properties of the SS-BLM are consistent with those of lipid vesicles from which they are formed. The possibility of modulating the size, lipid type and functionality, and mechanical stability makes these rigid liposomes very attractive candidates in biosensors, drug screening, and gene delivery-related applications. This is especially true in work with native vesicle membranes derived from living cells because the existing methods can only accommodate anionic membranes to a limited extent. |
| doi_str_mv | 10.1021/la9006982 |
| format | Article |
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| ispartof | Langmuir, 2009-05, Vol.25 (10), p.5455-5458 |
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| language | eng |
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| source | ACS Publications; MEDLINE |
| subjects | Biosensing Techniques - methods Cell-Derived Microparticles - chemistry Chemistry Colloidal state and disperse state Drug Evaluation, Preclinical - methods Exact sciences and technology Gene Transfer Techniques General and physical chemistry Lipid Bilayers - chemistry Membranes Phospholipids - chemistry Silicon Dioxide - chemistry Surface physical chemistry |
| title | Supported Bilayers Formed from Different Phospholipids on Spherical Silica Substrates |
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