Determining the Time Needed for the Vortex Method for Preparing Solvent-Free MALDI Samples of Low Molecular Mass Polymers
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is an important technique to characterize many different materials, including synthetic polymers. MALDI mass spectral data is used to determine the polymer average molecular weights, repeat units, and end groups. The development o...
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| Veröffentlicht in: | Journal of the American Society for Mass Spectrometry 2009-06, Vol.20 (6), p.1115-1118 |
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| creator | Hanton, Scott D. Stets, James R. |
| description | Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is an important technique to characterize many different materials, including synthetic polymers. MALDI mass spectral data is used to determine the polymer average molecular weights, repeat units, and end groups. The development of the vortex method of solvent-free sample preparation showed that remarkably short mixing times could prepare samples that yielded high quality MALDI mass spectra. In this paper, we use microscopy images and MALDI mass spectra to evaluate the mixing time required by the vortex method to produce mass spectra for low molecular mass polymer samples. Our results show that mixing times of as little as 10 s can generate homogeneous thin films that produce high quality mass spectra with S/N ∼ 100. In addition, ultrashort mixing times of only 2 s still produce samples with mostly smooth morphology and mass spectra with S/N ∼ 10.
SEM image from a sample of PMMA 2k prepared with IAA as the matrix utilizing only 10 seconds of vortex mixing. |
| doi_str_mv | 10.1016/j.jasms.2009.02.007 |
| format | Article |
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SEM image from a sample of PMMA 2k prepared with IAA as the matrix utilizing only 10 seconds of vortex mixing.</description><identifier>ISSN: 1044-0305</identifier><identifier>EISSN: 1879-1123</identifier><identifier>DOI: 10.1016/j.jasms.2009.02.007</identifier><identifier>PMID: 19303322</identifier><language>eng</language><publisher>New York: Elsevier Inc</publisher><subject>Analytical Chemistry ; Application Note ; Applied sciences ; Bioinformatics ; Biotechnology ; Chemistry ; Chemistry and Materials Science ; Desorption ; Exact sciences and technology ; Ionization ; Ions ; Lasers ; Mass spectra ; Mass spectrometry ; Mass spectroscopy ; Organic Chemistry ; Organic polymers ; Physicochemistry of polymers ; Polymers ; Properties and characterization ; Proteomics ; Structure, morphology and analysis ; Thin films ; Vortices</subject><ispartof>Journal of the American Society for Mass Spectrometry, 2009-06, Vol.20 (6), p.1115-1118</ispartof><rights>2009</rights><rights>American Society for Mass Spectrometry 2009</rights><rights>2009 INIST-CNRS</rights><rights>Journal of The American Society for Mass Spectrometry is a copyright of Springer, 2009.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-37668d0f0c1d39e09d44a5878f04464829f9ffa1b6aba23aabf9804cab6a628b3</citedby><cites>FETCH-LOGICAL-c491t-37668d0f0c1d39e09d44a5878f04464829f9ffa1b6aba23aabf9804cab6a628b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1016/j.jasms.2009.02.007$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1016/j.jasms.2009.02.007$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21549118$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19303322$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hanton, Scott D.</creatorcontrib><creatorcontrib>Stets, James R.</creatorcontrib><title>Determining the Time Needed for the Vortex Method for Preparing Solvent-Free MALDI Samples of Low Molecular Mass Polymers</title><title>Journal of the American Society for Mass Spectrometry</title><addtitle>J Am Soc Mass Spectrom</addtitle><addtitle>J Am Soc Mass Spectrom</addtitle><description>Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is an important technique to characterize many different materials, including synthetic polymers. MALDI mass spectral data is used to determine the polymer average molecular weights, repeat units, and end groups. The development of the vortex method of solvent-free sample preparation showed that remarkably short mixing times could prepare samples that yielded high quality MALDI mass spectra. In this paper, we use microscopy images and MALDI mass spectra to evaluate the mixing time required by the vortex method to produce mass spectra for low molecular mass polymer samples. Our results show that mixing times of as little as 10 s can generate homogeneous thin films that produce high quality mass spectra with S/N ∼ 100. In addition, ultrashort mixing times of only 2 s still produce samples with mostly smooth morphology and mass spectra with S/N ∼ 10.
SEM image from a sample of PMMA 2k prepared with IAA as the matrix utilizing only 10 seconds of vortex mixing.</description><subject>Analytical Chemistry</subject><subject>Application Note</subject><subject>Applied sciences</subject><subject>Bioinformatics</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Desorption</subject><subject>Exact sciences and technology</subject><subject>Ionization</subject><subject>Ions</subject><subject>Lasers</subject><subject>Mass spectra</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Organic Chemistry</subject><subject>Organic polymers</subject><subject>Physicochemistry of polymers</subject><subject>Polymers</subject><subject>Properties and characterization</subject><subject>Proteomics</subject><subject>Structure, morphology and analysis</subject><subject>Thin films</subject><subject>Vortices</subject><issn>1044-0305</issn><issn>1879-1123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU1v1DAQhiMEoh_wC5CQJQS3LOOPfPjAoWopVNqFSi1cLa8zpo6SOLWT0v33eLsrQBzKydboecczfrLsFYUFBVq-bxetjn1cMAC5ALYAqJ5kh7SuZE4p40_THYTIgUNxkB3F2ALQCmT1PDugkgPnjB1mmzOcMPRucMMPMt0guXY9ki-IDTbE-vBQ--7DhPdkhdON31UvA446bDNXvrvDYcrPAyJZnSzPLsiV7scOI_GWLP1PsvIdmrnTgax0jOTSd5seQ3yRPbO6i_hyfx5n384_Xp9-zpdfP12cnixzIySdcl6VZd2ABUMbLhFkI4Qu6qq2ablS1Exaaa2m61KvNeNar62sQRidCiWr1_w4e7frOwZ_O2OcVO-iwa7TA_o5qrJioi5Y_V-QCyGhFFUC3_wDtn4OQ1pCUVnQgnEQkCi-o0zwMQa0agyu12GjKKitQNWqB4FqK1ABU0lgSr3e957XPTZ_MntjCXi7B3Q0urNBD8bF3xyjRfo2ut1G7Lg4bj1h-GvIR9__sIthUnLnUiwah4PBxgU0k2q8ezT_C3Ugyms</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Hanton, Scott D.</creator><creator>Stets, James R.</creator><general>Elsevier Inc</general><general>Springer-Verlag</general><general>Elsevier</general><general>Springer Nature B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>Determining the Time Needed for the Vortex Method for Preparing Solvent-Free MALDI Samples of Low Molecular Mass Polymers</title><author>Hanton, Scott D. ; Stets, James R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-37668d0f0c1d39e09d44a5878f04464829f9ffa1b6aba23aabf9804cab6a628b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Analytical Chemistry</topic><topic>Application Note</topic><topic>Applied sciences</topic><topic>Bioinformatics</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Desorption</topic><topic>Exact sciences and technology</topic><topic>Ionization</topic><topic>Ions</topic><topic>Lasers</topic><topic>Mass spectra</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Organic Chemistry</topic><topic>Organic polymers</topic><topic>Physicochemistry of polymers</topic><topic>Polymers</topic><topic>Properties and characterization</topic><topic>Proteomics</topic><topic>Structure, morphology and analysis</topic><topic>Thin films</topic><topic>Vortices</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hanton, Scott D.</creatorcontrib><creatorcontrib>Stets, James R.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Society for Mass Spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hanton, Scott D.</au><au>Stets, James R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determining the Time Needed for the Vortex Method for Preparing Solvent-Free MALDI Samples of Low Molecular Mass Polymers</atitle><jtitle>Journal of the American Society for Mass Spectrometry</jtitle><stitle>J Am Soc Mass Spectrom</stitle><addtitle>J Am Soc Mass Spectrom</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>20</volume><issue>6</issue><spage>1115</spage><epage>1118</epage><pages>1115-1118</pages><issn>1044-0305</issn><eissn>1879-1123</eissn><abstract>Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is an important technique to characterize many different materials, including synthetic polymers. MALDI mass spectral data is used to determine the polymer average molecular weights, repeat units, and end groups. The development of the vortex method of solvent-free sample preparation showed that remarkably short mixing times could prepare samples that yielded high quality MALDI mass spectra. In this paper, we use microscopy images and MALDI mass spectra to evaluate the mixing time required by the vortex method to produce mass spectra for low molecular mass polymer samples. Our results show that mixing times of as little as 10 s can generate homogeneous thin films that produce high quality mass spectra with S/N ∼ 100. In addition, ultrashort mixing times of only 2 s still produce samples with mostly smooth morphology and mass spectra with S/N ∼ 10.
SEM image from a sample of PMMA 2k prepared with IAA as the matrix utilizing only 10 seconds of vortex mixing.</abstract><cop>New York</cop><pub>Elsevier Inc</pub><pmid>19303322</pmid><doi>10.1016/j.jasms.2009.02.007</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical Chemistry Application Note Applied sciences Bioinformatics Biotechnology Chemistry Chemistry and Materials Science Desorption Exact sciences and technology Ionization Ions Lasers Mass spectra Mass spectrometry Mass spectroscopy Organic Chemistry Organic polymers Physicochemistry of polymers Polymers Properties and characterization Proteomics Structure, morphology and analysis Thin films Vortices |
| title | Determining the Time Needed for the Vortex Method for Preparing Solvent-Free MALDI Samples of Low Molecular Mass Polymers |
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