Proliferation and β-tubulin for human aortic endothelial cells within gas-plasma scaffolds
We determined if human aortic endothelial cells (HAEC) enhanced proliferative and angiogenic phenotypes within gas-plasma treated bioresorbable D,L- poly lactic acid (D,L-PLA) three-dimensional scaffolds. 6 × 10 3 HAEC (N=120) were incubated for 6, 12 or 18 days within either non-treated control or...
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| Veröffentlicht in: | Cardiovascular radiation medicine 2004-07, Vol.5 (3), p.119-124 |
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| creator | Bailey, Steven R. Polan, Jodie L. Munoz, Oscar C. Agrawal, Mauli C. Goswami, Nilesh J. |
| description | We determined if
human
aortic
endothelial
cells (HAEC) enhanced proliferative and angiogenic phenotypes within gas-plasma treated bioresorbable D,L-
poly
lactic
acid (D,L-PLA) three-dimensional scaffolds.
6 × 10
3 HAEC (N=120) were incubated for 6, 12 or 18 days within either non-treated control or treated scaffolds. Before removing media, unstained wells were observed for apparent cell densities. Quantitative colorimetric WST-1 mitochondrial assays were determined for pooled conditioned media from both HAEC attached to wells and their respective HAEC-containing scaffolds. Fixed HAEC in scaffolds were examined using non-quantitative laser confocal microcopy with FITC-conjugated consensus, Types-I/II or Type-III β-tubulin.
WST-1 indicated that significantly (p |
| doi_str_mv | 10.1016/j.carrad.2004.08.001 |
| format | Article |
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human
aortic
endothelial
cells (HAEC) enhanced proliferative and angiogenic phenotypes within gas-plasma treated bioresorbable D,L-
poly
lactic
acid (D,L-PLA) three-dimensional scaffolds.
6 × 10
3 HAEC (N=120) were incubated for 6, 12 or 18 days within either non-treated control or treated scaffolds. Before removing media, unstained wells were observed for apparent cell densities. Quantitative colorimetric WST-1 mitochondrial assays were determined for pooled conditioned media from both HAEC attached to wells and their respective HAEC-containing scaffolds. Fixed HAEC in scaffolds were examined using non-quantitative laser confocal microcopy with FITC-conjugated consensus, Types-I/II or Type-III β-tubulin.
WST-1 indicated that significantly (p<0.05) less mitochondria were on cell culture plates than inside scaffolds but for different reasons. For example, a 12–18 days comparison between WST-1 and β-tubulin indicated that wells decreased because of overgrowth apotosis; whereas, mitochondrial activity inside treated scaffolds decreased with increased tubulogenesis. Observed with consensus and Type-I/II β-tubulin, HAEC-treated scaffolds exhibited increased cell-cell interconnections and angiogenic cords undergoing tubulogenesis to form vessels with central lumens as well as increased Type-III β-tubulin, predominantly in cells of smaller surface areas. Moreover, β-tubulin inside HAEC-treated scaffolds appeared in discrete cytoskeletal and podial regions; yet, β-tubulin for HAEC-control scaffolds was located in more diffuse cytoplasmic regions especially at 18 days.
HAEC-treated scaffolds undergo increased migration, proliferation, β-tubulin expression and quiescent cord formation. HAEC in scaffolds represent a potential model to study mechanisms for vascular cord progression into tubes. WST-1 does not represent accurate cell densities in three-dimensional scaffold matrices.</description><identifier>ISSN: 1522-1865</identifier><identifier>DOI: 10.1016/j.carrad.2004.08.001</identifier><identifier>PMID: 15721846</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Absorbable Implants ; Angiogenesis ; Aorta - cytology ; Aorta - drug effects ; Biocompatible Materials - chemistry ; Cell Adhesion ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Endothelium, Vascular - drug effects ; Gas-plasma treatment ; Gases ; Humans ; Intercellular Signaling Peptides and Proteins - pharmacology ; Mitochondria ; Phenotype ; Polyesters ; Scaffolds ; Staining and Labeling ; Surface Properties ; Tissue Engineering ; Tubulin - metabolism ; β-tubulin</subject><ispartof>Cardiovascular radiation medicine, 2004-07, Vol.5 (3), p.119-124</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c275t-62271d0513f57f1c3c270e80a572d8e22f5382b40c3fb6f4191cbceb436984963</citedby><cites>FETCH-LOGICAL-c275t-62271d0513f57f1c3c270e80a572d8e22f5382b40c3fb6f4191cbceb436984963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15721846$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bailey, Steven R.</creatorcontrib><creatorcontrib>Polan, Jodie L.</creatorcontrib><creatorcontrib>Munoz, Oscar C.</creatorcontrib><creatorcontrib>Agrawal, Mauli C.</creatorcontrib><creatorcontrib>Goswami, Nilesh J.</creatorcontrib><title>Proliferation and β-tubulin for human aortic endothelial cells within gas-plasma scaffolds</title><title>Cardiovascular radiation medicine</title><addtitle>Cardiovasc Radiat Med</addtitle><description>We determined if
human
aortic
endothelial
cells (HAEC) enhanced proliferative and angiogenic phenotypes within gas-plasma treated bioresorbable D,L-
poly
lactic
acid (D,L-PLA) three-dimensional scaffolds.
6 × 10
3 HAEC (N=120) were incubated for 6, 12 or 18 days within either non-treated control or treated scaffolds. Before removing media, unstained wells were observed for apparent cell densities. Quantitative colorimetric WST-1 mitochondrial assays were determined for pooled conditioned media from both HAEC attached to wells and their respective HAEC-containing scaffolds. Fixed HAEC in scaffolds were examined using non-quantitative laser confocal microcopy with FITC-conjugated consensus, Types-I/II or Type-III β-tubulin.
WST-1 indicated that significantly (p<0.05) less mitochondria were on cell culture plates than inside scaffolds but for different reasons. For example, a 12–18 days comparison between WST-1 and β-tubulin indicated that wells decreased because of overgrowth apotosis; whereas, mitochondrial activity inside treated scaffolds decreased with increased tubulogenesis. Observed with consensus and Type-I/II β-tubulin, HAEC-treated scaffolds exhibited increased cell-cell interconnections and angiogenic cords undergoing tubulogenesis to form vessels with central lumens as well as increased Type-III β-tubulin, predominantly in cells of smaller surface areas. Moreover, β-tubulin inside HAEC-treated scaffolds appeared in discrete cytoskeletal and podial regions; yet, β-tubulin for HAEC-control scaffolds was located in more diffuse cytoplasmic regions especially at 18 days.
HAEC-treated scaffolds undergo increased migration, proliferation, β-tubulin expression and quiescent cord formation. HAEC in scaffolds represent a potential model to study mechanisms for vascular cord progression into tubes. WST-1 does not represent accurate cell densities in three-dimensional scaffold matrices.</description><subject>Absorbable Implants</subject><subject>Angiogenesis</subject><subject>Aorta - cytology</subject><subject>Aorta - drug effects</subject><subject>Biocompatible Materials - chemistry</subject><subject>Cell Adhesion</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Gas-plasma treatment</subject><subject>Gases</subject><subject>Humans</subject><subject>Intercellular Signaling Peptides and Proteins - pharmacology</subject><subject>Mitochondria</subject><subject>Phenotype</subject><subject>Polyesters</subject><subject>Scaffolds</subject><subject>Staining and Labeling</subject><subject>Surface Properties</subject><subject>Tissue Engineering</subject><subject>Tubulin - metabolism</subject><subject>β-tubulin</subject><issn>1522-1865</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL1OwzAQgD2AaCm8AUKZ2BJsx3HSBQlV_EmVYICJwXLsM3XlxMVOQLwWD8Iz4aqV2JhOuvvu70PojOCCYMIv14WSIUhdUIxZgZsCY3KApqSiNCcNryboOMZ1SvJ5WR-hCalqShrGp-j1KXhnDQQ5WN9nstfZz3c-jO3obJ8ZH7LV2MlU8GGwKoNe-2EFzkqXKXAuZp92WCXyTcZ842TsZBaVNMY7HU_QoZEuwuk-ztDL7c3z4j5fPt49LK6XuaJ1NeSc0ppoXJHSVLUhqkxpDA2W6UjdAKWmKhvaMqxK03LDyJyoVkHLSj5v2JyXM3Sxm7sJ_n2EOIjOxu11sgc_RsFrympWkgSyHaiCjzGAEZtgOxm-BMFiK1KsxU6k2IoUuBHJWWo7388f2w70X9PeYgKudgCkLz8sBBGVhV6BtgHUILS3_2_4BWstiTA</recordid><startdate>200407</startdate><enddate>200407</enddate><creator>Bailey, Steven R.</creator><creator>Polan, Jodie L.</creator><creator>Munoz, Oscar C.</creator><creator>Agrawal, Mauli C.</creator><creator>Goswami, Nilesh J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200407</creationdate><title>Proliferation and β-tubulin for human aortic endothelial cells within gas-plasma scaffolds</title><author>Bailey, Steven R. ; Polan, Jodie L. ; Munoz, Oscar C. ; Agrawal, Mauli C. ; Goswami, Nilesh J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c275t-62271d0513f57f1c3c270e80a572d8e22f5382b40c3fb6f4191cbceb436984963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Absorbable Implants</topic><topic>Angiogenesis</topic><topic>Aorta - cytology</topic><topic>Aorta - drug effects</topic><topic>Biocompatible Materials - chemistry</topic><topic>Cell Adhesion</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Gas-plasma treatment</topic><topic>Gases</topic><topic>Humans</topic><topic>Intercellular Signaling Peptides and Proteins - pharmacology</topic><topic>Mitochondria</topic><topic>Phenotype</topic><topic>Polyesters</topic><topic>Scaffolds</topic><topic>Staining and Labeling</topic><topic>Surface Properties</topic><topic>Tissue Engineering</topic><topic>Tubulin - metabolism</topic><topic>β-tubulin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bailey, Steven R.</creatorcontrib><creatorcontrib>Polan, Jodie L.</creatorcontrib><creatorcontrib>Munoz, Oscar C.</creatorcontrib><creatorcontrib>Agrawal, Mauli C.</creatorcontrib><creatorcontrib>Goswami, Nilesh J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cardiovascular radiation medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bailey, Steven R.</au><au>Polan, Jodie L.</au><au>Munoz, Oscar C.</au><au>Agrawal, Mauli C.</au><au>Goswami, Nilesh J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proliferation and β-tubulin for human aortic endothelial cells within gas-plasma scaffolds</atitle><jtitle>Cardiovascular radiation medicine</jtitle><addtitle>Cardiovasc Radiat Med</addtitle><date>2004-07</date><risdate>2004</risdate><volume>5</volume><issue>3</issue><spage>119</spage><epage>124</epage><pages>119-124</pages><issn>1522-1865</issn><abstract>We determined if
human
aortic
endothelial
cells (HAEC) enhanced proliferative and angiogenic phenotypes within gas-plasma treated bioresorbable D,L-
poly
lactic
acid (D,L-PLA) three-dimensional scaffolds.
6 × 10
3 HAEC (N=120) were incubated for 6, 12 or 18 days within either non-treated control or treated scaffolds. Before removing media, unstained wells were observed for apparent cell densities. Quantitative colorimetric WST-1 mitochondrial assays were determined for pooled conditioned media from both HAEC attached to wells and their respective HAEC-containing scaffolds. Fixed HAEC in scaffolds were examined using non-quantitative laser confocal microcopy with FITC-conjugated consensus, Types-I/II or Type-III β-tubulin.
WST-1 indicated that significantly (p<0.05) less mitochondria were on cell culture plates than inside scaffolds but for different reasons. For example, a 12–18 days comparison between WST-1 and β-tubulin indicated that wells decreased because of overgrowth apotosis; whereas, mitochondrial activity inside treated scaffolds decreased with increased tubulogenesis. Observed with consensus and Type-I/II β-tubulin, HAEC-treated scaffolds exhibited increased cell-cell interconnections and angiogenic cords undergoing tubulogenesis to form vessels with central lumens as well as increased Type-III β-tubulin, predominantly in cells of smaller surface areas. Moreover, β-tubulin inside HAEC-treated scaffolds appeared in discrete cytoskeletal and podial regions; yet, β-tubulin for HAEC-control scaffolds was located in more diffuse cytoplasmic regions especially at 18 days.
HAEC-treated scaffolds undergo increased migration, proliferation, β-tubulin expression and quiescent cord formation. HAEC in scaffolds represent a potential model to study mechanisms for vascular cord progression into tubes. WST-1 does not represent accurate cell densities in three-dimensional scaffold matrices.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15721846</pmid><doi>10.1016/j.carrad.2004.08.001</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Cardiovascular radiation medicine, 2004-07, Vol.5 (3), p.119-124 |
| issn | 1522-1865 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Absorbable Implants Angiogenesis Aorta - cytology Aorta - drug effects Biocompatible Materials - chemistry Cell Adhesion Cell Movement Cell Proliferation Cells, Cultured Endothelium, Vascular - drug effects Gas-plasma treatment Gases Humans Intercellular Signaling Peptides and Proteins - pharmacology Mitochondria Phenotype Polyesters Scaffolds Staining and Labeling Surface Properties Tissue Engineering Tubulin - metabolism β-tubulin |
| title | Proliferation and β-tubulin for human aortic endothelial cells within gas-plasma scaffolds |
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