Effects of endocrine disrupters on sex steroid synthesis and metabolism pathways in fish
The interactions of estrogenic (nonylphenol, dicofol, atrazine), androgenic (organotins, phthalates, fenarimol) and anti-androgenic compounds (vinclozolin, diuron, p,p′-DDE) with key enzymatic activities involved in both synthesis and metabolism of sex hormones was investigated. Carp testicular micr...
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| Veröffentlicht in: | The Journal of steroid biochemistry and molecular biology 2004-12, Vol.92 (5), p.485-494 |
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| creator | Thibaut, Rémi Porte, Cinta |
| description | The interactions of estrogenic (nonylphenol, dicofol, atrazine), androgenic (organotins, phthalates, fenarimol) and anti-androgenic compounds (vinclozolin, diuron,
p,p′-DDE) with key enzymatic activities involved in both synthesis and metabolism of sex hormones was investigated. Carp testicular microsomes incubated in the presence of androstenedione and different xenobiotics evidenced higher sensitivity of 5α-reductase activity than 17β-hydroxysteroid dehydrogenase activity towards those chemicals. Dicofol, organotins and phthalates were among the most effective inhibitors. In contrast, ovarian synthesis of maturation-inducing hormones (20α- and 20β-hydroxysteroid dehydrogenase activities) were enhanced by nonylphenol, dicofol, fenarimol and
p,p′-DDE. Metabolic clearance pathways of hormones were also affected. Fenarimol, nonylphenol and triphenyltin inhibited the glucuronidation of testosterone and estradiol at concentrations as low as 10, 50 and 100
μM, respectively. Triphenyltin, tributyltin and nonylphenol were also inhibitors of estradiol sulfation with IC
50 values of 17, 18 and 41
μM. Overall, the data indicates the interaction of selected chemicals with key enzymatic pathways involved in both synthesis and metabolism of sex hormones. This interference might be one of the underlying mechanisms for the reported hormonal disrupting properties of the tested compounds, and might finally affect physiological processes such as gamete growth and maturation. |
| doi_str_mv | 10.1016/j.jsbmb.2004.10.008 |
| format | Article |
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p,p′-DDE) with key enzymatic activities involved in both synthesis and metabolism of sex hormones was investigated. Carp testicular microsomes incubated in the presence of androstenedione and different xenobiotics evidenced higher sensitivity of 5α-reductase activity than 17β-hydroxysteroid dehydrogenase activity towards those chemicals. Dicofol, organotins and phthalates were among the most effective inhibitors. In contrast, ovarian synthesis of maturation-inducing hormones (20α- and 20β-hydroxysteroid dehydrogenase activities) were enhanced by nonylphenol, dicofol, fenarimol and
p,p′-DDE. Metabolic clearance pathways of hormones were also affected. Fenarimol, nonylphenol and triphenyltin inhibited the glucuronidation of testosterone and estradiol at concentrations as low as 10, 50 and 100
μM, respectively. Triphenyltin, tributyltin and nonylphenol were also inhibitors of estradiol sulfation with IC
50 values of 17, 18 and 41
μM. Overall, the data indicates the interaction of selected chemicals with key enzymatic pathways involved in both synthesis and metabolism of sex hormones. This interference might be one of the underlying mechanisms for the reported hormonal disrupting properties of the tested compounds, and might finally affect physiological processes such as gamete growth and maturation.</description><identifier>ISSN: 0960-0760</identifier><identifier>EISSN: 1879-1220</identifier><identifier>DOI: 10.1016/j.jsbmb.2004.10.008</identifier><identifier>PMID: 15698553</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>17β-HSD ; 20α/β-HSD ; 5α-Reductase ; Androstenedione - pharmacology ; Animals ; Biological and medical sciences ; Carps - metabolism ; Endocrine disrupters ; Endocrine System - drug effects ; Endocrine System - metabolism ; Estradiol-SULT ; Female ; Fish ; Fundamental and applied biological sciences. Psychology ; Glucuronosyltransferase - metabolism ; Liver - enzymology ; Male ; Microsomes - enzymology ; Ovary - drug effects ; Ovary - metabolism ; Sex Attractants - biosynthesis ; Sex Attractants - metabolism ; Sulfotransferases - metabolism ; Testis - drug effects ; Testis - metabolism ; UGT ; Vertebrates: endocrinology ; Xenobiotics - pharmacology</subject><ispartof>The Journal of steroid biochemistry and molecular biology, 2004-12, Vol.92 (5), p.485-494</ispartof><rights>2004 Elsevier Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-edd28dc8aee06d6aaef2a0f3788b482cf2884fe4f94fc0a88e3bfbcb48b8ef0e3</citedby><cites>FETCH-LOGICAL-c453t-edd28dc8aee06d6aaef2a0f3788b482cf2884fe4f94fc0a88e3bfbcb48b8ef0e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jsbmb.2004.10.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16474556$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15698553$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thibaut, Rémi</creatorcontrib><creatorcontrib>Porte, Cinta</creatorcontrib><title>Effects of endocrine disrupters on sex steroid synthesis and metabolism pathways in fish</title><title>The Journal of steroid biochemistry and molecular biology</title><addtitle>J Steroid Biochem Mol Biol</addtitle><description>The interactions of estrogenic (nonylphenol, dicofol, atrazine), androgenic (organotins, phthalates, fenarimol) and anti-androgenic compounds (vinclozolin, diuron,
p,p′-DDE) with key enzymatic activities involved in both synthesis and metabolism of sex hormones was investigated. Carp testicular microsomes incubated in the presence of androstenedione and different xenobiotics evidenced higher sensitivity of 5α-reductase activity than 17β-hydroxysteroid dehydrogenase activity towards those chemicals. Dicofol, organotins and phthalates were among the most effective inhibitors. In contrast, ovarian synthesis of maturation-inducing hormones (20α- and 20β-hydroxysteroid dehydrogenase activities) were enhanced by nonylphenol, dicofol, fenarimol and
p,p′-DDE. Metabolic clearance pathways of hormones were also affected. Fenarimol, nonylphenol and triphenyltin inhibited the glucuronidation of testosterone and estradiol at concentrations as low as 10, 50 and 100
μM, respectively. Triphenyltin, tributyltin and nonylphenol were also inhibitors of estradiol sulfation with IC
50 values of 17, 18 and 41
μM. Overall, the data indicates the interaction of selected chemicals with key enzymatic pathways involved in both synthesis and metabolism of sex hormones. This interference might be one of the underlying mechanisms for the reported hormonal disrupting properties of the tested compounds, and might finally affect physiological processes such as gamete growth and maturation.</description><subject>17β-HSD</subject><subject>20α/β-HSD</subject><subject>5α-Reductase</subject><subject>Androstenedione - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carps - metabolism</subject><subject>Endocrine disrupters</subject><subject>Endocrine System - drug effects</subject><subject>Endocrine System - metabolism</subject><subject>Estradiol-SULT</subject><subject>Female</subject><subject>Fish</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucuronosyltransferase - metabolism</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Microsomes - enzymology</subject><subject>Ovary - drug effects</subject><subject>Ovary - metabolism</subject><subject>Sex Attractants - biosynthesis</subject><subject>Sex Attractants - metabolism</subject><subject>Sulfotransferases - metabolism</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><subject>UGT</subject><subject>Vertebrates: endocrinology</subject><subject>Xenobiotics - pharmacology</subject><issn>0960-0760</issn><issn>1879-1220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcGKFDEQhoMo7uzqEwiSi956rHTS6czBgyzrKix4UfAW0kmFydCdHlM9uvP2ZpyBvXkq6s9XRfGFsTcC1gKE_rBb72iYhnULoGqyBjDP2EqYftOItoXnbAUbDQ30Gq7YNdEOAKQU_Ut2JTq9MV0nV-znXYzoF-Jz5JjD7EvKyEOictgvWGqeOeEjp9rMKXA65mWLlIi7HPiEixvmMdHE927Z_nFH4inzmGj7ir2IbiR8fak37Mfnu--3X5qHb_dfbz89NF51cmkwhNYEbxwi6KCdw9g6iLI3ZlCm9bE1RkVUcaOiB2cMyiEOvr4NBiOgvGHvz3v3Zf51QFrslMjjOLqM84Gs7lslpFAVlGfQl5moYLT7kiZXjlaAPQm1O_tPqD0JPYVVaJ16e1l_GCYMTzMXgxV4dwEceTfG4rJP9MRp1auu05X7eOawyvidsFjyCbPHkEr9ABvm9N9D_gJovJen</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>Thibaut, Rémi</creator><creator>Porte, Cinta</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041201</creationdate><title>Effects of endocrine disrupters on sex steroid synthesis and metabolism pathways in fish</title><author>Thibaut, Rémi ; Porte, Cinta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-edd28dc8aee06d6aaef2a0f3788b482cf2884fe4f94fc0a88e3bfbcb48b8ef0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>17β-HSD</topic><topic>20α/β-HSD</topic><topic>5α-Reductase</topic><topic>Androstenedione - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carps - metabolism</topic><topic>Endocrine disrupters</topic><topic>Endocrine System - drug effects</topic><topic>Endocrine System - metabolism</topic><topic>Estradiol-SULT</topic><topic>Female</topic><topic>Fish</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucuronosyltransferase - metabolism</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Microsomes - enzymology</topic><topic>Ovary - drug effects</topic><topic>Ovary - metabolism</topic><topic>Sex Attractants - biosynthesis</topic><topic>Sex Attractants - metabolism</topic><topic>Sulfotransferases - metabolism</topic><topic>Testis - drug effects</topic><topic>Testis - metabolism</topic><topic>UGT</topic><topic>Vertebrates: endocrinology</topic><topic>Xenobiotics - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thibaut, Rémi</creatorcontrib><creatorcontrib>Porte, Cinta</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thibaut, Rémi</au><au>Porte, Cinta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of endocrine disrupters on sex steroid synthesis and metabolism pathways in fish</atitle><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle><addtitle>J Steroid Biochem Mol Biol</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>92</volume><issue>5</issue><spage>485</spage><epage>494</epage><pages>485-494</pages><issn>0960-0760</issn><eissn>1879-1220</eissn><abstract>The interactions of estrogenic (nonylphenol, dicofol, atrazine), androgenic (organotins, phthalates, fenarimol) and anti-androgenic compounds (vinclozolin, diuron,
p,p′-DDE) with key enzymatic activities involved in both synthesis and metabolism of sex hormones was investigated. Carp testicular microsomes incubated in the presence of androstenedione and different xenobiotics evidenced higher sensitivity of 5α-reductase activity than 17β-hydroxysteroid dehydrogenase activity towards those chemicals. Dicofol, organotins and phthalates were among the most effective inhibitors. In contrast, ovarian synthesis of maturation-inducing hormones (20α- and 20β-hydroxysteroid dehydrogenase activities) were enhanced by nonylphenol, dicofol, fenarimol and
p,p′-DDE. Metabolic clearance pathways of hormones were also affected. Fenarimol, nonylphenol and triphenyltin inhibited the glucuronidation of testosterone and estradiol at concentrations as low as 10, 50 and 100
μM, respectively. Triphenyltin, tributyltin and nonylphenol were also inhibitors of estradiol sulfation with IC
50 values of 17, 18 and 41
μM. Overall, the data indicates the interaction of selected chemicals with key enzymatic pathways involved in both synthesis and metabolism of sex hormones. This interference might be one of the underlying mechanisms for the reported hormonal disrupting properties of the tested compounds, and might finally affect physiological processes such as gamete growth and maturation.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15698553</pmid><doi>10.1016/j.jsbmb.2004.10.008</doi><tpages>10</tpages></addata></record> |
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| subjects | 17β-HSD 20α/β-HSD 5α-Reductase Androstenedione - pharmacology Animals Biological and medical sciences Carps - metabolism Endocrine disrupters Endocrine System - drug effects Endocrine System - metabolism Estradiol-SULT Female Fish Fundamental and applied biological sciences. Psychology Glucuronosyltransferase - metabolism Liver - enzymology Male Microsomes - enzymology Ovary - drug effects Ovary - metabolism Sex Attractants - biosynthesis Sex Attractants - metabolism Sulfotransferases - metabolism Testis - drug effects Testis - metabolism UGT Vertebrates: endocrinology Xenobiotics - pharmacology |
| title | Effects of endocrine disrupters on sex steroid synthesis and metabolism pathways in fish |
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