Caspase activity inhibition delays programmed spermatogenic cell death in vitro

Programmed cell death or apoptosis was analyzed in rat Sertoli-spermatogonial cell cocultures prepared from 2-9 day old rats using time-lapse video microscopy, a cell viability fluorescence microscopy assay, immunocytochemical markers, and cell-permeable caspase inhibitory peptides with reversible a...

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Veröffentlicht in:	Archives of Histology and Cytology 2004, Vol.67(4), pp.315-324
Hauptverfasser:	Tres, Laura L., Rosselot, Carolina, Kierszenbaum, Abraham L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - metabolism Animals Apoptosis Biomarkers - metabolism Caspase Inhibitors Cell Survival Cells, Cultured Coculture Techniques Culture Media, Serum-Free Cytoskeleton - metabolism Fluorescent Antibody Technique, Indirect Growth Hormone - metabolism Immunohistochemistry Male Microscopy, Video Phagocytosis Proto-Oncogene Proteins c-bcl-2 - metabolism Rats Sertoli Cells - metabolism Sertoli Cells - physiology Spermatozoa - metabolism Spermatozoa - physiology Time Factors
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