Cdx2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells
In our recent study showing a correlation between Brm-deficiency and undifferentiated status of gastric cancer, we found that the Brm-type SWI/SNF complex is required for villin expression. To elucidate intestinal villin regulation more precisely, we here analyzed structure and function of the promo...
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| Veröffentlicht in: | Experimental cell research 2009-06, Vol.315 (10), p.1779-1789 |
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| creator | Yamamichi, Nobutake Inada, Ken-ichi Furukawa, Chihiro Sakurai, Kouhei Tando, Toshio Ishizaka, Aya Haraguchi, Takeshi Mizutani, Taketoshi Fujishiro, Mitsuhiro Shimomura, Ryoichi Oka, Masashi Ichinose, Masao Tsutsumi, Yutaka Omata, Masao Iba, Hideo |
| description | In our recent study showing a correlation between Brm-deficiency and undifferentiated status of gastric cancer, we found that the Brm-type SWI/SNF complex is required for
villin expression. To elucidate intestinal
villin regulation more precisely, we here analyzed structure and function of the promoter of human
villin. About 1.1 kb upstream of the determined major transcription start site, we identified a highly conserved region (HCR-Cdx) among mammals, which contains two binding sites for Cdx. Expression analyses of 30 human gastrointestinal cell lines suggested that
villin is regulated by Cdx2. Introduction of
Cdx family genes into colorectal SW480 cells revealed that
villin is strongly induced strongly by Cdx2, moderately by Cdx1, and marginally by Cdx4. Knockdown of Cdx2 in SW480 cells caused a clear downregulation of
villin, and reporter assays showed that HCR-Cdx is crucial for Cdx2-dependent and Brm-dependent
villin expression. Immunohistochemical analyses of gastric intestinal metaplasia and cancer revealed that villin and Cdx2 expression are tightly coupled. GST pull-down assays demonstrated a direct interaction between Cdx2 and several SWI/SNF subunits. Chromatin immunoprecipitation analyses showed the recruitment of Cdx2 and Brm around HCR-Cdx. From these results, we concluded that Cdx2 regulates intestinal
villin expression through recruiting Brm-type SWI/SNF complex to the
villin promoter. |
| doi_str_mv | 10.1016/j.yexcr.2009.01.006 |
| format | Article |
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villin expression. To elucidate intestinal
villin regulation more precisely, we here analyzed structure and function of the promoter of human
villin. About 1.1 kb upstream of the determined major transcription start site, we identified a highly conserved region (HCR-Cdx) among mammals, which contains two binding sites for Cdx. Expression analyses of 30 human gastrointestinal cell lines suggested that
villin is regulated by Cdx2. Introduction of
Cdx family genes into colorectal SW480 cells revealed that
villin is strongly induced strongly by Cdx2, moderately by Cdx1, and marginally by Cdx4. Knockdown of Cdx2 in SW480 cells caused a clear downregulation of
villin, and reporter assays showed that HCR-Cdx is crucial for Cdx2-dependent and Brm-dependent
villin expression. Immunohistochemical analyses of gastric intestinal metaplasia and cancer revealed that villin and Cdx2 expression are tightly coupled. GST pull-down assays demonstrated a direct interaction between Cdx2 and several SWI/SNF subunits. Chromatin immunoprecipitation analyses showed the recruitment of Cdx2 and Brm around HCR-Cdx. From these results, we concluded that Cdx2 regulates intestinal
villin expression through recruiting Brm-type SWI/SNF complex to the
villin promoter.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/j.yexcr.2009.01.006</identifier><identifier>PMID: 19371634</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Base Sequence ; Binding Sites ; Blotting, Western ; Brm ; Cdx2 ; CDX2 Transcription Factor ; Cell Line, Tumor ; Cellular biology ; Chromatin Assembly and Disassembly ; Conserved Sequence ; Digestive system ; Gastric cancer ; Gastrointestinal Tract - cytology ; Gastrointestinal Tract - metabolism ; Gene expression ; Gene Expression Regulation ; Genes, Reporter ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Humans ; Microfilament Proteins - genetics ; Molecular Sequence Data ; Promoter Regions, Genetic - genetics ; Protein Binding ; Protein Subunits - metabolism ; Proteins ; SWI/SNF complex ; Transcription Factors - metabolism ; Transcription Initiation Site ; Villin</subject><ispartof>Experimental cell research, 2009-06, Vol.315 (10), p.1779-1789</ispartof><rights>2009 Elsevier Inc.</rights><rights>Copyright © 2009 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-c5b9f0e177324f2157b04f8454642dc6ff15574bf5adffad64d18a9ea5cb79093</citedby><cites>FETCH-LOGICAL-c450t-c5b9f0e177324f2157b04f8454642dc6ff15574bf5adffad64d18a9ea5cb79093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014482709000329$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19371634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamamichi, Nobutake</creatorcontrib><creatorcontrib>Inada, Ken-ichi</creatorcontrib><creatorcontrib>Furukawa, Chihiro</creatorcontrib><creatorcontrib>Sakurai, Kouhei</creatorcontrib><creatorcontrib>Tando, Toshio</creatorcontrib><creatorcontrib>Ishizaka, Aya</creatorcontrib><creatorcontrib>Haraguchi, Takeshi</creatorcontrib><creatorcontrib>Mizutani, Taketoshi</creatorcontrib><creatorcontrib>Fujishiro, Mitsuhiro</creatorcontrib><creatorcontrib>Shimomura, Ryoichi</creatorcontrib><creatorcontrib>Oka, Masashi</creatorcontrib><creatorcontrib>Ichinose, Masao</creatorcontrib><creatorcontrib>Tsutsumi, Yutaka</creatorcontrib><creatorcontrib>Omata, Masao</creatorcontrib><creatorcontrib>Iba, Hideo</creatorcontrib><title>Cdx2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>In our recent study showing a correlation between Brm-deficiency and undifferentiated status of gastric cancer, we found that the Brm-type SWI/SNF complex is required for
villin expression. To elucidate intestinal
villin regulation more precisely, we here analyzed structure and function of the promoter of human
villin. About 1.1 kb upstream of the determined major transcription start site, we identified a highly conserved region (HCR-Cdx) among mammals, which contains two binding sites for Cdx. Expression analyses of 30 human gastrointestinal cell lines suggested that
villin is regulated by Cdx2. Introduction of
Cdx family genes into colorectal SW480 cells revealed that
villin is strongly induced strongly by Cdx2, moderately by Cdx1, and marginally by Cdx4. Knockdown of Cdx2 in SW480 cells caused a clear downregulation of
villin, and reporter assays showed that HCR-Cdx is crucial for Cdx2-dependent and Brm-dependent
villin expression. Immunohistochemical analyses of gastric intestinal metaplasia and cancer revealed that villin and Cdx2 expression are tightly coupled. GST pull-down assays demonstrated a direct interaction between Cdx2 and several SWI/SNF subunits. Chromatin immunoprecipitation analyses showed the recruitment of Cdx2 and Brm around HCR-Cdx. From these results, we concluded that Cdx2 regulates intestinal
villin expression through recruiting Brm-type SWI/SNF complex to the
villin promoter.</description><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Blotting, Western</subject><subject>Brm</subject><subject>Cdx2</subject><subject>CDX2 Transcription Factor</subject><subject>Cell Line, Tumor</subject><subject>Cellular biology</subject><subject>Chromatin Assembly and Disassembly</subject><subject>Conserved Sequence</subject><subject>Digestive system</subject><subject>Gastric cancer</subject><subject>Gastrointestinal Tract - cytology</subject><subject>Gastrointestinal Tract - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genes, Reporter</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Microfilament Proteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein Binding</subject><subject>Protein Subunits - metabolism</subject><subject>Proteins</subject><subject>SWI/SNF complex</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription Initiation Site</subject><subject>Villin</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9r3DAQxUVpaTZpP0GhiB56szOSZck-9NAuTRoIzSEpPQpZHiVa_K-Svex--2q7C4UcchoGfvPm8R4hHxjkDJi83OR73NmQc4A6B5YDyFdkxaCGjAvOX5MVABOZqLg6I-cxbgCgqph8S85YXSgmC7EiT-t2x6kZWjo_If0W-mzeT0jvf99c3v-8onbspw53aY4TBjP7LXZ7GvBx6cyMdOu7zg8Ud1PAGP040LQ9mjiH0Q8zxtkPpqMWuy6-I2-c6SK-P80L8uvq-8P6R3Z7d32z_nqbWVHCnNmyqR0gU6rgwnFWqgaEq0QppOCtlc6xslSicaVpnTOtFC2rTI2mtI2qoS4uyOej7hTGP0uyoHsfDw7MgOMStVS8SAKQwE_PwM24hOQ3alYLqZQQMkHFEbJhjDGg01PwvQl7zUAfWtAb_a8FfWhBA9OphXT18SS9ND22_29OsSfgyxHAlMTWY9DRehwstj6gnXU7-hcf_AXEv5pg</recordid><startdate>20090610</startdate><enddate>20090610</enddate><creator>Yamamichi, Nobutake</creator><creator>Inada, Ken-ichi</creator><creator>Furukawa, Chihiro</creator><creator>Sakurai, Kouhei</creator><creator>Tando, Toshio</creator><creator>Ishizaka, Aya</creator><creator>Haraguchi, Takeshi</creator><creator>Mizutani, Taketoshi</creator><creator>Fujishiro, Mitsuhiro</creator><creator>Shimomura, Ryoichi</creator><creator>Oka, Masashi</creator><creator>Ichinose, Masao</creator><creator>Tsutsumi, Yutaka</creator><creator>Omata, Masao</creator><creator>Iba, Hideo</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090610</creationdate><title>Cdx2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells</title><author>Yamamichi, Nobutake ; Inada, Ken-ichi ; Furukawa, Chihiro ; Sakurai, Kouhei ; Tando, Toshio ; Ishizaka, Aya ; Haraguchi, Takeshi ; Mizutani, Taketoshi ; Fujishiro, Mitsuhiro ; Shimomura, Ryoichi ; Oka, Masashi ; Ichinose, Masao ; Tsutsumi, Yutaka ; Omata, Masao ; Iba, Hideo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-c5b9f0e177324f2157b04f8454642dc6ff15574bf5adffad64d18a9ea5cb79093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Blotting, Western</topic><topic>Brm</topic><topic>Cdx2</topic><topic>CDX2 Transcription Factor</topic><topic>Cell Line, Tumor</topic><topic>Cellular biology</topic><topic>Chromatin Assembly and Disassembly</topic><topic>Conserved Sequence</topic><topic>Digestive system</topic><topic>Gastric cancer</topic><topic>Gastrointestinal Tract - cytology</topic><topic>Gastrointestinal Tract - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genes, Reporter</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Microfilament Proteins - genetics</topic><topic>Molecular Sequence Data</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein Binding</topic><topic>Protein Subunits - metabolism</topic><topic>Proteins</topic><topic>SWI/SNF complex</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription Initiation Site</topic><topic>Villin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamamichi, Nobutake</creatorcontrib><creatorcontrib>Inada, Ken-ichi</creatorcontrib><creatorcontrib>Furukawa, Chihiro</creatorcontrib><creatorcontrib>Sakurai, Kouhei</creatorcontrib><creatorcontrib>Tando, Toshio</creatorcontrib><creatorcontrib>Ishizaka, Aya</creatorcontrib><creatorcontrib>Haraguchi, Takeshi</creatorcontrib><creatorcontrib>Mizutani, Taketoshi</creatorcontrib><creatorcontrib>Fujishiro, Mitsuhiro</creatorcontrib><creatorcontrib>Shimomura, Ryoichi</creatorcontrib><creatorcontrib>Oka, Masashi</creatorcontrib><creatorcontrib>Ichinose, Masao</creatorcontrib><creatorcontrib>Tsutsumi, Yutaka</creatorcontrib><creatorcontrib>Omata, Masao</creatorcontrib><creatorcontrib>Iba, Hideo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamamichi, Nobutake</au><au>Inada, Ken-ichi</au><au>Furukawa, Chihiro</au><au>Sakurai, Kouhei</au><au>Tando, Toshio</au><au>Ishizaka, Aya</au><au>Haraguchi, Takeshi</au><au>Mizutani, Taketoshi</au><au>Fujishiro, Mitsuhiro</au><au>Shimomura, Ryoichi</au><au>Oka, Masashi</au><au>Ichinose, Masao</au><au>Tsutsumi, Yutaka</au><au>Omata, Masao</au><au>Iba, Hideo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cdx2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2009-06-10</date><risdate>2009</risdate><volume>315</volume><issue>10</issue><spage>1779</spage><epage>1789</epage><pages>1779-1789</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>In our recent study showing a correlation between Brm-deficiency and undifferentiated status of gastric cancer, we found that the Brm-type SWI/SNF complex is required for
villin expression. To elucidate intestinal
villin regulation more precisely, we here analyzed structure and function of the promoter of human
villin. About 1.1 kb upstream of the determined major transcription start site, we identified a highly conserved region (HCR-Cdx) among mammals, which contains two binding sites for Cdx. Expression analyses of 30 human gastrointestinal cell lines suggested that
villin is regulated by Cdx2. Introduction of
Cdx family genes into colorectal SW480 cells revealed that
villin is strongly induced strongly by Cdx2, moderately by Cdx1, and marginally by Cdx4. Knockdown of Cdx2 in SW480 cells caused a clear downregulation of
villin, and reporter assays showed that HCR-Cdx is crucial for Cdx2-dependent and Brm-dependent
villin expression. Immunohistochemical analyses of gastric intestinal metaplasia and cancer revealed that villin and Cdx2 expression are tightly coupled. GST pull-down assays demonstrated a direct interaction between Cdx2 and several SWI/SNF subunits. Chromatin immunoprecipitation analyses showed the recruitment of Cdx2 and Brm around HCR-Cdx. From these results, we concluded that Cdx2 regulates intestinal
villin expression through recruiting Brm-type SWI/SNF complex to the
villin promoter.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19371634</pmid><doi>10.1016/j.yexcr.2009.01.006</doi><tpages>11</tpages></addata></record> |
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| subjects | Base Sequence Binding Sites Blotting, Western Brm Cdx2 CDX2 Transcription Factor Cell Line, Tumor Cellular biology Chromatin Assembly and Disassembly Conserved Sequence Digestive system Gastric cancer Gastrointestinal Tract - cytology Gastrointestinal Tract - metabolism Gene expression Gene Expression Regulation Genes, Reporter Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Microfilament Proteins - genetics Molecular Sequence Data Promoter Regions, Genetic - genetics Protein Binding Protein Subunits - metabolism Proteins SWI/SNF complex Transcription Factors - metabolism Transcription Initiation Site Villin |
| title | Cdx2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells |
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