Evaluation of window cohabitation of DNA sequencing errors and lowest PHRED quality values

When analyzing sequencing reads, it is important to distinguish between putative correct and wrong bases. An open question is how a PHRED quality value is capable of identifying the miscalled bases and if there is a quality cutoff that allows mapping of most errors. Considering the fact that a low q...

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Veröffentlicht in:	Genetics and molecular research 2004-12, Vol.3 (4), p.483-492
Hauptverfasser:	Prosdocimi, Francisco, Peixoto, Fabiano Cruz, Ortega, José Miguel
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Base Sequence Databases, Genetic - standards Genome, Human Humans Molecular Sequence Data Open Reading Frames Quality Control Sequence Alignment Sequence Analysis, DNA - methods Sequence Analysis, DNA - standards Software
Online-Zugang:	Volltext
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