Effect of vimentin on reactive gliosis: In vitro and in vivo analysis
Reactive gliosis is a prominent result of many types of insult to the central nervous system (CNS) and leads to the formation of glial scar that impedes the regeneration of axons. The intermediate filament protein vimentin is found in pathology of the CNS, mainly in the vicinity of injuries to the C...
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| Veröffentlicht in: | Journal of neurotrauma 2004-11, Vol.21 (11), p.1671-1682 |
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| description | Reactive gliosis is a prominent result of many types of insult to the central nervous system (CNS) and leads to the formation of glial scar that impedes the regeneration of axons. The intermediate filament protein vimentin is found in pathology of the CNS, mainly in the vicinity of injuries to the CNS. In the present study we investigated the role of vimentin in the formation of glial scars in vitro and in vivo by using immunohistochemistry, Western blot analysis, and in situ hybridization. In vitro experiments showed that the intensity of immunofluorescent labeling for vimentin and glial fibrillary acidic protein (GFAP) was consistently decreased in astrocytes after transfection with a retrovirus carrying antisense complementary DNA (cDNA) for vimentin. Transfection also inhibited the growth of astrocytes and decreased the expression of vimentin mRNA. In vivo studies demonstrated that transfection with the retrovirus carrying the antisense cDNA vimentin inhibited the upregulation of vimentin and GFAP in stab wounds in rat cerebrum. These results suggest that vimentin may play a key role in the formation of glial scars in the CNS. |
| doi_str_mv | 10.1089/neu.2004.21.1671 |
| format | Article |
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The intermediate filament protein vimentin is found in pathology of the CNS, mainly in the vicinity of injuries to the CNS. In the present study we investigated the role of vimentin in the formation of glial scars in vitro and in vivo by using immunohistochemistry, Western blot analysis, and in situ hybridization. In vitro experiments showed that the intensity of immunofluorescent labeling for vimentin and glial fibrillary acidic protein (GFAP) was consistently decreased in astrocytes after transfection with a retrovirus carrying antisense complementary DNA (cDNA) for vimentin. Transfection also inhibited the growth of astrocytes and decreased the expression of vimentin mRNA. In vivo studies demonstrated that transfection with the retrovirus carrying the antisense cDNA vimentin inhibited the upregulation of vimentin and GFAP in stab wounds in rat cerebrum. These results suggest that vimentin may play a key role in the formation of glial scars in the CNS.</description><identifier>ISSN: 0897-7151</identifier><identifier>EISSN: 1557-9042</identifier><identifier>DOI: 10.1089/neu.2004.21.1671</identifier><identifier>PMID: 15684657</identifier><identifier>CODEN: JNEUE4</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>Animals ; Animals, Newborn ; Antisense Elements (Genetics) - pharmacology ; Astrocytes - cytology ; Astrocytes - metabolism ; Biological and medical sciences ; Brain Injuries - metabolism ; Brain Injuries - pathology ; Brain Injuries - physiopathology ; Cells, Cultured ; Central Nervous System - injuries ; Central Nervous System - pathology ; Central Nervous System - physiopathology ; Cicatrix - metabolism ; Cicatrix - pathology ; Cicatrix - physiopathology ; DNA, Complementary - genetics ; Down-Regulation - physiology ; Fluorescent Antibody Technique ; Genetic Vectors ; Glial Fibrillary Acidic Protein - metabolism ; Gliosis - metabolism ; Gliosis - pathology ; Gliosis - physiopathology ; Infections ; Injuries of the nervous system and the skull. Diseases due to physical agents ; Medical sciences ; Mice ; Nervous system ; NIH 3T3 Cells ; Proteins ; Rats ; Rats, Wistar ; Transfection ; Traumas. Diseases due to physical agents ; Vimentin - antagonists & inhibitors ; Vimentin - genetics ; Vimentin - metabolism ; Viruses</subject><ispartof>Journal of neurotrauma, 2004-11, Vol.21 (11), p.1671-1682</ispartof><rights>2005 INIST-CNRS</rights><rights>(©) Copyright 2004, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-4a9d9341c07bcee7fac9524e4ae04dd03e2ca4842345885b9a8bb18d722ad6343</citedby><cites>FETCH-LOGICAL-c352t-4a9d9341c07bcee7fac9524e4ae04dd03e2ca4842345885b9a8bb18d722ad6343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3042,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16285331$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15684657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Jiangkai</creatorcontrib><creatorcontrib>Cai, Wenqin</creatorcontrib><title>Effect of vimentin on reactive gliosis: In vitro and in vivo analysis</title><title>Journal of neurotrauma</title><addtitle>J Neurotrauma</addtitle><description>Reactive gliosis is a prominent result of many types of insult to the central nervous system (CNS) and leads to the formation of glial scar that impedes the regeneration of axons. The intermediate filament protein vimentin is found in pathology of the CNS, mainly in the vicinity of injuries to the CNS. In the present study we investigated the role of vimentin in the formation of glial scars in vitro and in vivo by using immunohistochemistry, Western blot analysis, and in situ hybridization. In vitro experiments showed that the intensity of immunofluorescent labeling for vimentin and glial fibrillary acidic protein (GFAP) was consistently decreased in astrocytes after transfection with a retrovirus carrying antisense complementary DNA (cDNA) for vimentin. Transfection also inhibited the growth of astrocytes and decreased the expression of vimentin mRNA. In vivo studies demonstrated that transfection with the retrovirus carrying the antisense cDNA vimentin inhibited the upregulation of vimentin and GFAP in stab wounds in rat cerebrum. These results suggest that vimentin may play a key role in the formation of glial scars in the CNS.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Antisense Elements (Genetics) - pharmacology</subject><subject>Astrocytes - cytology</subject><subject>Astrocytes - metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain Injuries - metabolism</subject><subject>Brain Injuries - pathology</subject><subject>Brain Injuries - physiopathology</subject><subject>Cells, Cultured</subject><subject>Central Nervous System - injuries</subject><subject>Central Nervous System - pathology</subject><subject>Central Nervous System - physiopathology</subject><subject>Cicatrix - metabolism</subject><subject>Cicatrix - pathology</subject><subject>Cicatrix - physiopathology</subject><subject>DNA, Complementary - genetics</subject><subject>Down-Regulation - physiology</subject><subject>Fluorescent Antibody Technique</subject><subject>Genetic Vectors</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Gliosis - metabolism</subject><subject>Gliosis - pathology</subject><subject>Gliosis - physiopathology</subject><subject>Infections</subject><subject>Injuries of the nervous system and the skull. Diseases due to physical agents</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Nervous system</subject><subject>NIH 3T3 Cells</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Transfection</subject><subject>Traumas. Diseases due to physical agents</subject><subject>Vimentin - antagonists & inhibitors</subject><subject>Vimentin - genetics</subject><subject>Vimentin - metabolism</subject><subject>Viruses</subject><issn>0897-7151</issn><issn>1557-9042</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpd0M1LwzAYBvAgipvTuycpgt5a853Wm4ypg4EXPYc0TSWjS2bSDvbfm7KC4CkJ-T0vLw8AtwgWCJbVkzNDgSGkBUYF4gKdgTliTOQVpPgczBMRuUAMzcBVjFsIEeFYXIIZYryknIk5WK3a1ug-8212sDvjeusy77JglO7twWTfnfXRxuds7RLog8-UazI7Pg7jXXXH9H0NLlrVRXMznQvw9br6XL7nm4-39fJlk2vCcJ9TVTUVoUhDUWtjRKt0xTA1VBlImwYSg7WiJcWEsrJkdaXKukZlIzBWDSeULMDjae4--J_BxF7ubNSm65QzfoiSixTlBCV4_w9u_RDStlFiSBlKjiUET0gHH2MwrdwHu1PhKBGUY78y9SvHfiVGcuw3Re6muUO9M81fYCo0gYcJqKhV1wbltI1_juOSkbTgLze_ggs</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Lin, Jiangkai</creator><creator>Cai, Wenqin</creator><general>Liebert</general><general>Mary Ann Liebert, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Effect of vimentin on reactive gliosis: In vitro and in vivo analysis</title><author>Lin, Jiangkai ; Cai, Wenqin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-4a9d9341c07bcee7fac9524e4ae04dd03e2ca4842345885b9a8bb18d722ad6343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Antisense Elements (Genetics) - pharmacology</topic><topic>Astrocytes - cytology</topic><topic>Astrocytes - metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain Injuries - metabolism</topic><topic>Brain Injuries - pathology</topic><topic>Brain Injuries - physiopathology</topic><topic>Cells, Cultured</topic><topic>Central Nervous System - injuries</topic><topic>Central Nervous System - pathology</topic><topic>Central Nervous System - physiopathology</topic><topic>Cicatrix - metabolism</topic><topic>Cicatrix - pathology</topic><topic>Cicatrix - physiopathology</topic><topic>DNA, Complementary - genetics</topic><topic>Down-Regulation - physiology</topic><topic>Fluorescent Antibody Technique</topic><topic>Genetic Vectors</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Gliosis - metabolism</topic><topic>Gliosis - pathology</topic><topic>Gliosis - physiopathology</topic><topic>Infections</topic><topic>Injuries of the nervous system and the skull. Diseases due to physical agents</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Nervous system</topic><topic>NIH 3T3 Cells</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Transfection</topic><topic>Traumas. Diseases due to physical agents</topic><topic>Vimentin - antagonists & inhibitors</topic><topic>Vimentin - genetics</topic><topic>Vimentin - metabolism</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Jiangkai</creatorcontrib><creatorcontrib>Cai, Wenqin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurotrauma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Jiangkai</au><au>Cai, Wenqin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of vimentin on reactive gliosis: In vitro and in vivo analysis</atitle><jtitle>Journal of neurotrauma</jtitle><addtitle>J Neurotrauma</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>21</volume><issue>11</issue><spage>1671</spage><epage>1682</epage><pages>1671-1682</pages><issn>0897-7151</issn><eissn>1557-9042</eissn><coden>JNEUE4</coden><abstract>Reactive gliosis is a prominent result of many types of insult to the central nervous system (CNS) and leads to the formation of glial scar that impedes the regeneration of axons. The intermediate filament protein vimentin is found in pathology of the CNS, mainly in the vicinity of injuries to the CNS. In the present study we investigated the role of vimentin in the formation of glial scars in vitro and in vivo by using immunohistochemistry, Western blot analysis, and in situ hybridization. In vitro experiments showed that the intensity of immunofluorescent labeling for vimentin and glial fibrillary acidic protein (GFAP) was consistently decreased in astrocytes after transfection with a retrovirus carrying antisense complementary DNA (cDNA) for vimentin. Transfection also inhibited the growth of astrocytes and decreased the expression of vimentin mRNA. In vivo studies demonstrated that transfection with the retrovirus carrying the antisense cDNA vimentin inhibited the upregulation of vimentin and GFAP in stab wounds in rat cerebrum. These results suggest that vimentin may play a key role in the formation of glial scars in the CNS.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>15684657</pmid><doi>10.1089/neu.2004.21.1671</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Animals, Newborn Antisense Elements (Genetics) - pharmacology Astrocytes - cytology Astrocytes - metabolism Biological and medical sciences Brain Injuries - metabolism Brain Injuries - pathology Brain Injuries - physiopathology Cells, Cultured Central Nervous System - injuries Central Nervous System - pathology Central Nervous System - physiopathology Cicatrix - metabolism Cicatrix - pathology Cicatrix - physiopathology DNA, Complementary - genetics Down-Regulation - physiology Fluorescent Antibody Technique Genetic Vectors Glial Fibrillary Acidic Protein - metabolism Gliosis - metabolism Gliosis - pathology Gliosis - physiopathology Infections Injuries of the nervous system and the skull. Diseases due to physical agents Medical sciences Mice Nervous system NIH 3T3 Cells Proteins Rats Rats, Wistar Transfection Traumas. Diseases due to physical agents Vimentin - antagonists & inhibitors Vimentin - genetics Vimentin - metabolism Viruses |
| title | Effect of vimentin on reactive gliosis: In vitro and in vivo analysis |
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