Detection and expression of the phosphonate transporter gene phnD in marine and freshwater picocyanobacteria

We describe a PCR-based assay designed to detect expression of the phosphonate assimilation gene phnD from picocyanobacteria. The phnD gene encodes the phosphonate binding protein of the ABC-type phosphonate transporter, present in many of the picocyanobacterial genome sequences. Detection of phnD e...

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Veröffentlicht in:	Environmental microbiology 2009-05, Vol.11 (5), p.1314-1324
Hauptverfasser:	Ilikchyan, Irina N, McKay, R. Michael L, Zehr, Jonathan P, Dyhrman, Sonya T, Bullerjahn, George S
Format:	Artikel
Sprache:	eng
Schlagworte:	ABC transporters Bacterial Proteins - genetics Bacterial Proteins - metabolism DNA primers DNA, Bacterial - chemistry DNA, Bacterial - genetics Fresh Water - microbiology freshwater gene expression Gene Expression Profiling gene expression regulation genes herbicides Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Molecular Sequence Data nutrient availability nutrient deficiencies oceans Organophosphonates - metabolism phosphonates phosphorus Phylogeny plankton polymerase chain reaction Prochlorococcus Prochlorococcus - enzymology Prochlorococcus - genetics Reverse Transcriptase Polymerase Chain Reaction - methods RNA Seawater - microbiology Sequence Analysis, DNA Sequence Homology, Amino Acid Synechococcus Synechococcus - enzymology Synechococcus - genetics
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