A model to induce low temperature trauma for in vitro astrogliosis study
Astrogliosis is an inevitable and rapid response of astrocytes to physical, chemical and pathological injuries. To study astrogliosis, we developed a reproducible in vitro model in which low temperature injury to cultured astrocytes could be induced by placing the culture dish onto a copper pipe pre...
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| Veröffentlicht in: | Neurochemical research 2004-11, Vol.29 (11), p.2171-2176 |
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| container_title | Neurochemical research |
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| creator | Yu, Albert Cheung Hoi Wu, Bing Yi Liu, Rong Yu Li, Qiang Li, Yue Xin Wong, Pui-Fan Liu, Shuang Lau, Lok Ting Fung, Yin Wan Wendy |
| description | Astrogliosis is an inevitable and rapid response of astrocytes to physical, chemical and pathological injuries. To study astrogliosis, we developed a reproducible in vitro model in which low temperature injury to cultured astrocytes could be induced by placing the culture dish onto a copper pipe pre-cooled by liquid nitrogen. Using this model, the relationship between the temperature decline and the severity of cellular damage was analyzed. An increase in the expression of some known injury-related proteins, such as glial fibrillary acidic protein (GFAP), immediate early response genes (IEGs), and heat shock proteins 70 (HSP70), was demonstrated in astrocytes after low temperature trauma. With the use of this low temperature trauma model, the flexibility in the temperature control and injury area may allow researchers to evaluate cryotherapy and cryosurgery, which could be applicable to future development of quality health care. |
| doi_str_mv | 10.1007/s11064-004-6891-z |
| format | Article |
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To study astrogliosis, we developed a reproducible in vitro model in which low temperature injury to cultured astrocytes could be induced by placing the culture dish onto a copper pipe pre-cooled by liquid nitrogen. Using this model, the relationship between the temperature decline and the severity of cellular damage was analyzed. An increase in the expression of some known injury-related proteins, such as glial fibrillary acidic protein (GFAP), immediate early response genes (IEGs), and heat shock proteins 70 (HSP70), was demonstrated in astrocytes after low temperature trauma. With the use of this low temperature trauma model, the flexibility in the temperature control and injury area may allow researchers to evaluate cryotherapy and cryosurgery, which could be applicable to future development of quality health care.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/s11064-004-6891-z</identifier><identifier>PMID: 15662852</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Animals ; Astrocytes - pathology ; Cells, Cultured ; Cold Temperature - adverse effects ; Genes, Immediate-Early - genetics ; Glial Fibrillary Acidic Protein - biosynthesis ; Glial Fibrillary Acidic Protein - genetics ; HSP70 Heat-Shock Proteins - biosynthesis ; Immunohistochemistry ; Mice ; Proto-Oncogene Proteins c-fos - biosynthesis ; Proto-Oncogene Proteins c-fos - genetics ; Proto-Oncogene Proteins c-jun - biosynthesis ; Proto-Oncogene Proteins c-jun - genetics ; Proto-Oncogene Proteins c-myc - biosynthesis ; Proto-Oncogene Proteins c-myc - genetics</subject><ispartof>Neurochemical research, 2004-11, Vol.29 (11), p.2171-2176</ispartof><rights>Copyright (c) 2004 Springer Science+Business Media, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-109561198801153064f74bf82342c2b51c64b05c8933e88ba53982d3c7c7d5a53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15662852$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Albert Cheung Hoi</creatorcontrib><creatorcontrib>Wu, Bing Yi</creatorcontrib><creatorcontrib>Liu, Rong Yu</creatorcontrib><creatorcontrib>Li, Qiang</creatorcontrib><creatorcontrib>Li, Yue Xin</creatorcontrib><creatorcontrib>Wong, Pui-Fan</creatorcontrib><creatorcontrib>Liu, Shuang</creatorcontrib><creatorcontrib>Lau, Lok Ting</creatorcontrib><creatorcontrib>Fung, Yin Wan Wendy</creatorcontrib><title>A model to induce low temperature trauma for in vitro astrogliosis study</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><description>Astrogliosis is an inevitable and rapid response of astrocytes to physical, chemical and pathological injuries. To study astrogliosis, we developed a reproducible in vitro model in which low temperature injury to cultured astrocytes could be induced by placing the culture dish onto a copper pipe pre-cooled by liquid nitrogen. Using this model, the relationship between the temperature decline and the severity of cellular damage was analyzed. An increase in the expression of some known injury-related proteins, such as glial fibrillary acidic protein (GFAP), immediate early response genes (IEGs), and heat shock proteins 70 (HSP70), was demonstrated in astrocytes after low temperature trauma. With the use of this low temperature trauma model, the flexibility in the temperature control and injury area may allow researchers to evaluate cryotherapy and cryosurgery, which could be applicable to future development of quality health care.</description><subject>Animals</subject><subject>Astrocytes - pathology</subject><subject>Cells, Cultured</subject><subject>Cold Temperature - adverse effects</subject><subject>Genes, Immediate-Early - genetics</subject><subject>Glial Fibrillary Acidic Protein - biosynthesis</subject><subject>Glial Fibrillary Acidic Protein - genetics</subject><subject>HSP70 Heat-Shock Proteins - biosynthesis</subject><subject>Immunohistochemistry</subject><subject>Mice</subject><subject>Proto-Oncogene Proteins c-fos - biosynthesis</subject><subject>Proto-Oncogene Proteins c-fos - genetics</subject><subject>Proto-Oncogene Proteins c-jun - biosynthesis</subject><subject>Proto-Oncogene Proteins c-jun - genetics</subject><subject>Proto-Oncogene Proteins c-myc - biosynthesis</subject><subject>Proto-Oncogene Proteins c-myc - 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Academic</collection><jtitle>Neurochemical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Albert Cheung Hoi</au><au>Wu, Bing Yi</au><au>Liu, Rong Yu</au><au>Li, Qiang</au><au>Li, Yue Xin</au><au>Wong, Pui-Fan</au><au>Liu, Shuang</au><au>Lau, Lok Ting</au><au>Fung, Yin Wan Wendy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A model to induce low temperature trauma for in vitro astrogliosis study</atitle><jtitle>Neurochemical research</jtitle><addtitle>Neurochem Res</addtitle><date>2004-11</date><risdate>2004</risdate><volume>29</volume><issue>11</issue><spage>2171</spage><epage>2176</epage><pages>2171-2176</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><abstract>Astrogliosis is an inevitable and rapid response of astrocytes to physical, chemical and pathological injuries. To study astrogliosis, we developed a reproducible in vitro model in which low temperature injury to cultured astrocytes could be induced by placing the culture dish onto a copper pipe pre-cooled by liquid nitrogen. Using this model, the relationship between the temperature decline and the severity of cellular damage was analyzed. An increase in the expression of some known injury-related proteins, such as glial fibrillary acidic protein (GFAP), immediate early response genes (IEGs), and heat shock proteins 70 (HSP70), was demonstrated in astrocytes after low temperature trauma. With the use of this low temperature trauma model, the flexibility in the temperature control and injury area may allow researchers to evaluate cryotherapy and cryosurgery, which could be applicable to future development of quality health care.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>15662852</pmid><doi>10.1007/s11064-004-6891-z</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Astrocytes - pathology Cells, Cultured Cold Temperature - adverse effects Genes, Immediate-Early - genetics Glial Fibrillary Acidic Protein - biosynthesis Glial Fibrillary Acidic Protein - genetics HSP70 Heat-Shock Proteins - biosynthesis Immunohistochemistry Mice Proto-Oncogene Proteins c-fos - biosynthesis Proto-Oncogene Proteins c-fos - genetics Proto-Oncogene Proteins c-jun - biosynthesis Proto-Oncogene Proteins c-jun - genetics Proto-Oncogene Proteins c-myc - biosynthesis Proto-Oncogene Proteins c-myc - genetics |
| title | A model to induce low temperature trauma for in vitro astrogliosis study |
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