Increased Number of Mesenchymal Stem Cell-like Cells in Peripheral Blood of Patients with Bone Sarcomas
Background and Aims The number of peripheral blood mesenchymal stem cells (PBMSCs) may increase under pathological conditions. We sought to compare the number of MSC-like cells in the peripheral blood of patients with bone sarcomas with healthy controls and to analyze related cytokines in the periph...
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| Veröffentlicht in: | Archives of medical research 2009-04, Vol.40 (3), p.163-168 |
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| creator | Bian, Zhen-Yu Li, Gang Gan, Yao-Kai Hao, Yong-Qiang Xu, Wen-Ting Tang, Ting-Ting |
| description | Background and Aims The number of peripheral blood mesenchymal stem cells (PBMSCs) may increase under pathological conditions. We sought to compare the number of MSC-like cells in the peripheral blood of patients with bone sarcomas with healthy controls and to analyze related cytokines in the peripheral blood plasma. Methods Peripheral blood mononuclear cells (PBMNs) of patients with bone sarcomas and control subjects were isolated for culture and analyzed by flow cytometry for MSC phenotype. Cytokines in the plasma obtained after cell separation were analyzed using enzyme-linked immunosorbent assay (ELISA). Annexin-V and β -galactosidase staining were used to investigate whether the cells died from apoptosis or senescence. Results Flow cytometric analysis demonstrated an >9-fold increase in the number of cells with MSC-like phenotypes (CD34– , CD45– , CD105+ ) in patients with bone sarcomas compared with control subjects ( p |
| doi_str_mv | 10.1016/j.arcmed.2009.01.002 |
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We sought to compare the number of MSC-like cells in the peripheral blood of patients with bone sarcomas with healthy controls and to analyze related cytokines in the peripheral blood plasma. Methods Peripheral blood mononuclear cells (PBMNs) of patients with bone sarcomas and control subjects were isolated for culture and analyzed by flow cytometry for MSC phenotype. Cytokines in the plasma obtained after cell separation were analyzed using enzyme-linked immunosorbent assay (ELISA). Annexin-V and β -galactosidase staining were used to investigate whether the cells died from apoptosis or senescence. Results Flow cytometric analysis demonstrated an >9-fold increase in the number of cells with MSC-like phenotypes (CD34– , CD45– , CD105+ ) in patients with bone sarcomas compared with control subjects ( p <0.05). ELISA results showed that concentrations of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) in patients with bone sarcomas were statistically higher than those in the control subjects ( p <0.05), whereas there was no significant difference in plasma concentrations of leptin and stromal cell-derived factor 1 between the two groups. A significant, positive correlation between the percentages of PBMSC-like cells and concentrations of HGF in all samples (R = 0.618; p = 0.011). Annexin-V staining of MSC-like cells was positive, whereas β -galactosidase staining was negative. Conclusions Peripheral blood of patients with bone sarcomas has more cells with MSC phenotypes than blood of healthy persons. The increased number is accompanied by increased HGF and VEGF in the plasma.</description><identifier>ISSN: 0188-4409</identifier><identifier>EISSN: 1873-5487</identifier><identifier>DOI: 10.1016/j.arcmed.2009.01.002</identifier><identifier>PMID: 19427966</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Annexin A5 - metabolism ; Apoptosis - physiology ; beta-Galactosidase - metabolism ; Bone Neoplasms - blood ; Bone Neoplasms - pathology ; Bone sarcoma ; Cell Count ; Chemokine CXCL12 - blood ; Female ; Hepatocyte growth factor ; Hepatocyte Growth Factor - blood ; Humans ; Internal Medicine ; Leptin - blood ; Male ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - pathology ; Middle Aged ; Peripheral blood ; Sarcoma - blood ; Sarcoma - pathology ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - blood ; Young Adult</subject><ispartof>Archives of medical research, 2009-04, Vol.40 (3), p.163-168</ispartof><rights>IMSS</rights><rights>2009 IMSS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-3f76576b5676d39ccd1d62c0dd91f054aca974b7dbfafbed6cdcfb5a13d140d63</citedby><cites>FETCH-LOGICAL-c415t-3f76576b5676d39ccd1d62c0dd91f054aca974b7dbfafbed6cdcfb5a13d140d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.arcmed.2009.01.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19427966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bian, Zhen-Yu</creatorcontrib><creatorcontrib>Li, Gang</creatorcontrib><creatorcontrib>Gan, Yao-Kai</creatorcontrib><creatorcontrib>Hao, Yong-Qiang</creatorcontrib><creatorcontrib>Xu, Wen-Ting</creatorcontrib><creatorcontrib>Tang, Ting-Ting</creatorcontrib><title>Increased Number of Mesenchymal Stem Cell-like Cells in Peripheral Blood of Patients with Bone Sarcomas</title><title>Archives of medical research</title><addtitle>Arch Med Res</addtitle><description>Background and Aims The number of peripheral blood mesenchymal stem cells (PBMSCs) may increase under pathological conditions. We sought to compare the number of MSC-like cells in the peripheral blood of patients with bone sarcomas with healthy controls and to analyze related cytokines in the peripheral blood plasma. Methods Peripheral blood mononuclear cells (PBMNs) of patients with bone sarcomas and control subjects were isolated for culture and analyzed by flow cytometry for MSC phenotype. Cytokines in the plasma obtained after cell separation were analyzed using enzyme-linked immunosorbent assay (ELISA). Annexin-V and β -galactosidase staining were used to investigate whether the cells died from apoptosis or senescence. Results Flow cytometric analysis demonstrated an >9-fold increase in the number of cells with MSC-like phenotypes (CD34– , CD45– , CD105+ ) in patients with bone sarcomas compared with control subjects ( p <0.05). ELISA results showed that concentrations of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) in patients with bone sarcomas were statistically higher than those in the control subjects ( p <0.05), whereas there was no significant difference in plasma concentrations of leptin and stromal cell-derived factor 1 between the two groups. A significant, positive correlation between the percentages of PBMSC-like cells and concentrations of HGF in all samples (R = 0.618; p = 0.011). Annexin-V staining of MSC-like cells was positive, whereas β -galactosidase staining was negative. Conclusions Peripheral blood of patients with bone sarcomas has more cells with MSC phenotypes than blood of healthy persons. The increased number is accompanied by increased HGF and VEGF in the plasma.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Annexin A5 - metabolism</subject><subject>Apoptosis - physiology</subject><subject>beta-Galactosidase - metabolism</subject><subject>Bone Neoplasms - blood</subject><subject>Bone Neoplasms - pathology</subject><subject>Bone sarcoma</subject><subject>Cell Count</subject><subject>Chemokine CXCL12 - blood</subject><subject>Female</subject><subject>Hepatocyte growth factor</subject><subject>Hepatocyte Growth Factor - blood</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Leptin - blood</subject><subject>Male</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - pathology</subject><subject>Middle Aged</subject><subject>Peripheral blood</subject><subject>Sarcoma - blood</subject><subject>Sarcoma - pathology</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - blood</subject><subject>Young Adult</subject><issn>0188-4409</issn><issn>1873-5487</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS1ERZfCP0DIJ25Jx4ljJxckuipQqYVKC2fLsSest0m82EnR_nscdsWhl8qHseT3nme-IeQdg5wBE5e7XAczoM0LgCYHlgMUL8iK1bLMKl7Ll2QFrK4zzqE5J69j3AFAzYV8Rc5ZwwvZCLEiv25GE1BHtPTbPLQYqO_oHUYczfYw6J5uJhzoGvs-690D_rtF6kZ6j8HttxiS5Kr33i6-ez05HKdI_7hpS6_8iHSTmvSDjm_IWaf7iG9P9YL8_Hz9Y_01u_3-5Wb96TYznFVTVnZSVFK0lZDClo0xlllRGLC2YR1UXBvdSN5K23a6a9EKY03XVpqVlnGworwgH465--B_zxgnNbhoUtN6RD9HJWRRyLKskpAfhSb4GAN2ah_coMNBMVALYLVTR8BqAayAqQQ42d6f8ud2eftvOhFNgo9HAaYpHx0GFU2CYtC6gGZS1rvnfngaYHo3OqP7Bzxg3Pk5jImgYioWCtRmWfKy43SSnYnyL3dVpAE</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Bian, Zhen-Yu</creator><creator>Li, Gang</creator><creator>Gan, Yao-Kai</creator><creator>Hao, Yong-Qiang</creator><creator>Xu, Wen-Ting</creator><creator>Tang, Ting-Ting</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090401</creationdate><title>Increased Number of Mesenchymal Stem Cell-like Cells in Peripheral Blood of Patients with Bone Sarcomas</title><author>Bian, Zhen-Yu ; Li, Gang ; Gan, Yao-Kai ; Hao, Yong-Qiang ; Xu, Wen-Ting ; Tang, Ting-Ting</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-3f76576b5676d39ccd1d62c0dd91f054aca974b7dbfafbed6cdcfb5a13d140d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Annexin A5 - metabolism</topic><topic>Apoptosis - physiology</topic><topic>beta-Galactosidase - metabolism</topic><topic>Bone Neoplasms - blood</topic><topic>Bone Neoplasms - pathology</topic><topic>Bone sarcoma</topic><topic>Cell Count</topic><topic>Chemokine CXCL12 - blood</topic><topic>Female</topic><topic>Hepatocyte growth factor</topic><topic>Hepatocyte Growth Factor - blood</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Leptin - blood</topic><topic>Male</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - pathology</topic><topic>Middle Aged</topic><topic>Peripheral blood</topic><topic>Sarcoma - blood</topic><topic>Sarcoma - pathology</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A - blood</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bian, Zhen-Yu</creatorcontrib><creatorcontrib>Li, Gang</creatorcontrib><creatorcontrib>Gan, Yao-Kai</creatorcontrib><creatorcontrib>Hao, Yong-Qiang</creatorcontrib><creatorcontrib>Xu, Wen-Ting</creatorcontrib><creatorcontrib>Tang, Ting-Ting</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of medical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bian, Zhen-Yu</au><au>Li, Gang</au><au>Gan, Yao-Kai</au><au>Hao, Yong-Qiang</au><au>Xu, Wen-Ting</au><au>Tang, Ting-Ting</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Number of Mesenchymal Stem Cell-like Cells in Peripheral Blood of Patients with Bone Sarcomas</atitle><jtitle>Archives of medical research</jtitle><addtitle>Arch Med Res</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>40</volume><issue>3</issue><spage>163</spage><epage>168</epage><pages>163-168</pages><issn>0188-4409</issn><eissn>1873-5487</eissn><abstract>Background and Aims The number of peripheral blood mesenchymal stem cells (PBMSCs) may increase under pathological conditions. We sought to compare the number of MSC-like cells in the peripheral blood of patients with bone sarcomas with healthy controls and to analyze related cytokines in the peripheral blood plasma. Methods Peripheral blood mononuclear cells (PBMNs) of patients with bone sarcomas and control subjects were isolated for culture and analyzed by flow cytometry for MSC phenotype. Cytokines in the plasma obtained after cell separation were analyzed using enzyme-linked immunosorbent assay (ELISA). Annexin-V and β -galactosidase staining were used to investigate whether the cells died from apoptosis or senescence. Results Flow cytometric analysis demonstrated an >9-fold increase in the number of cells with MSC-like phenotypes (CD34– , CD45– , CD105+ ) in patients with bone sarcomas compared with control subjects ( p <0.05). ELISA results showed that concentrations of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) in patients with bone sarcomas were statistically higher than those in the control subjects ( p <0.05), whereas there was no significant difference in plasma concentrations of leptin and stromal cell-derived factor 1 between the two groups. A significant, positive correlation between the percentages of PBMSC-like cells and concentrations of HGF in all samples (R = 0.618; p = 0.011). Annexin-V staining of MSC-like cells was positive, whereas β -galactosidase staining was negative. Conclusions Peripheral blood of patients with bone sarcomas has more cells with MSC phenotypes than blood of healthy persons. The increased number is accompanied by increased HGF and VEGF in the plasma.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19427966</pmid><doi>10.1016/j.arcmed.2009.01.002</doi><tpages>6</tpages></addata></record> |
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| subjects | Adolescent Adult Annexin A5 - metabolism Apoptosis - physiology beta-Galactosidase - metabolism Bone Neoplasms - blood Bone Neoplasms - pathology Bone sarcoma Cell Count Chemokine CXCL12 - blood Female Hepatocyte growth factor Hepatocyte Growth Factor - blood Humans Internal Medicine Leptin - blood Male Mesenchymal stem cells Mesenchymal Stromal Cells - pathology Middle Aged Peripheral blood Sarcoma - blood Sarcoma - pathology Vascular endothelial growth factor Vascular Endothelial Growth Factor A - blood Young Adult |
| title | Increased Number of Mesenchymal Stem Cell-like Cells in Peripheral Blood of Patients with Bone Sarcomas |
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