Expression of Matrix Metalloproteinases and Their Specific Inhibitors in Normal and Different Human Thyroid Tumor Cell Lines
In the present study we investigated, by means of zymography and reverse transcription-polymerase chain reaction (RT-PCR), the expression of different matrix metalloproteinases (MMPs) and of the specific tissue inhibitor of metalloproteinases [TIMPs] in human cell lines derived from normal thyrocyte...
Gespeichert in:
| Veröffentlicht in: | Thyroid (New York, N.Y.) N.Y.), 2004-11, Vol.14 (11), p.881-888 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 888 |
|---|---|
| container_issue | 11 |
| container_start_page | 881 |
| container_title | Thyroid (New York, N.Y.) |
| container_volume | 14 |
| creator | Baldini, E. Toller, M. Graziano, F.M. Russo, F.P. Pepe, M. Biordi, L. Marchioni, E. Curcio, F. Ulisse, S. Ambesi-Impiombato, F.S. D’Armiento, M. |
| description | In the present study we investigated, by means of zymography and reverse transcription-polymerase chain reaction
(RT-PCR), the expression of different matrix metalloproteinases (MMPs) and of the specific tissue inhibitor
of metalloproteinases [TIMPs] in human cell lines derived from normal thyrocytes (HTU5), follicular
adenoma (HTU42), and follicular (FTC-133), papillary (B-CPAP), and anaplastic (CAL-62, 8305C) thyroid carcinomas.
We demonstrated that normal thyrocytes constitutively express MMP-1, MMP-2, MMP-10, MMP-14,
and TIMP-1, TIMP-2, TIMP-3, and TIMP-4, and this pattern of expression is profoundly modified in all thyroid
tumor-derived cell lines. Analysis of the gelatinolytic activity in the different cell supernatants showed that the
expressions of MMP-2 and MMP-9 are, respectively, increased or induced in all the neoplastic cell lines, except
in CAL-62. Caseinolytic activity was found only in the supernatants of the 8305C and B-CPAP cells. Using RTPCR
analysis we detected an increased expression of MMP-1 in cell lines derived from papillary and from one
(8305C) of the two anaplastic carcinomas. MMP-13 mRNA was expressed only in the 8305C, FTC-133, and BCPAP
cells. Among stromelysins, MMP-3 mRNA could not be detected in any cell line, while MMP-10 mRNA
was expressed in all of them, although at variable levels. MMP-11 mRNA was absent in normal and follicular
adenoma derived thyrocytes and induced in all carcinoma cell types. The expression of MMP-14 (MT1-MMP)
mRNA was found significantly increased in all thyroid tumor cell lines with respect to HTU5 and HTU42 cells.
The expression of TIMP-1 and TIMP-2 mRNAs was maintained in all cell lines tested, while that of TIMP-3 was
lost in both anaplastic carcinoma cell lines and that of TIMP-4 was absent in the CAL-62. In conclusion, our
data demonstrated a differential expression of MMPs and TIMPs in different thyroid tumor cell types with respect
to normal thyrocytes. In particular, the induction of MMP-11 in all thyroid-derived carcinoma cell lines
studied and of MMP-13 in all but one may represent, if confirmed in other thyroid tumor-derived cell lines and
in thyroid tumor tissues, a new marker of thyrocyte transformation. |
| doi_str_mv | 10.1089/thy.2004.14.881 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67226487</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67226487</sourcerecordid><originalsourceid>FETCH-LOGICAL-c333t-341b1653298e210e8e399e25add3607fa2d9ff880126a10abe488067818bb4e3</originalsourceid><addsrcrecordid>eNpdkM9v2yAYhtHUaW2znXerOPXmlB824GOVZmuldDssd4TtD4XKhgyw1Ej940eWaJV24uPT876CB6GvlCwpUe1d3h2WjJB6SeulUvQDuqJNI6uWSHlRZtKQSrJGXKLrlF4IoUJJ_gld0kZIKkVzhd7Wr_sIKbngcbD42eToXvEzZDOOYR9DBudNgoSNH_B2By7iX3vonXU9fvI717kcYsLO4x8hTmb8yz04ayGCz_hxnowvuUMMruTnKUS8gnHEG-chfUYfrRkTfDmfC7T9tt6uHqvNz-9Pq_tN1XPOc8Vr2lHRcNYqYJSAAt62wBozDFwQaQ0bWmuVIpQJQ4npoC4XIRVVXVcDX6DbU235z-8ZUtaTS315hfEQ5qSFZEzUxcwC3Z3APoaUIli9j24y8aAp0UffuvjWR9-a1rr4Lombc_XcTTC882fBBahOwHFtvB8ddBDzP_D_wj9WUI0S</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67226487</pqid></control><display><type>article</type><title>Expression of Matrix Metalloproteinases and Their Specific Inhibitors in Normal and Different Human Thyroid Tumor Cell Lines</title><source>Mary Ann Liebert Online Subscription</source><source>MEDLINE</source><creator>Baldini, E. ; Toller, M. ; Graziano, F.M. ; Russo, F.P. ; Pepe, M. ; Biordi, L. ; Marchioni, E. ; Curcio, F. ; Ulisse, S. ; Ambesi-Impiombato, F.S. ; D’Armiento, M.</creator><creatorcontrib>Baldini, E. ; Toller, M. ; Graziano, F.M. ; Russo, F.P. ; Pepe, M. ; Biordi, L. ; Marchioni, E. ; Curcio, F. ; Ulisse, S. ; Ambesi-Impiombato, F.S. ; D’Armiento, M.</creatorcontrib><description>In the present study we investigated, by means of zymography and reverse transcription-polymerase chain reaction
(RT-PCR), the expression of different matrix metalloproteinases (MMPs) and of the specific tissue inhibitor
of metalloproteinases [TIMPs] in human cell lines derived from normal thyrocytes (HTU5), follicular
adenoma (HTU42), and follicular (FTC-133), papillary (B-CPAP), and anaplastic (CAL-62, 8305C) thyroid carcinomas.
We demonstrated that normal thyrocytes constitutively express MMP-1, MMP-2, MMP-10, MMP-14,
and TIMP-1, TIMP-2, TIMP-3, and TIMP-4, and this pattern of expression is profoundly modified in all thyroid
tumor-derived cell lines. Analysis of the gelatinolytic activity in the different cell supernatants showed that the
expressions of MMP-2 and MMP-9 are, respectively, increased or induced in all the neoplastic cell lines, except
in CAL-62. Caseinolytic activity was found only in the supernatants of the 8305C and B-CPAP cells. Using RTPCR
analysis we detected an increased expression of MMP-1 in cell lines derived from papillary and from one
(8305C) of the two anaplastic carcinomas. MMP-13 mRNA was expressed only in the 8305C, FTC-133, and BCPAP
cells. Among stromelysins, MMP-3 mRNA could not be detected in any cell line, while MMP-10 mRNA
was expressed in all of them, although at variable levels. MMP-11 mRNA was absent in normal and follicular
adenoma derived thyrocytes and induced in all carcinoma cell types. The expression of MMP-14 (MT1-MMP)
mRNA was found significantly increased in all thyroid tumor cell lines with respect to HTU5 and HTU42 cells.
The expression of TIMP-1 and TIMP-2 mRNAs was maintained in all cell lines tested, while that of TIMP-3 was
lost in both anaplastic carcinoma cell lines and that of TIMP-4 was absent in the CAL-62. In conclusion, our
data demonstrated a differential expression of MMPs and TIMPs in different thyroid tumor cell types with respect
to normal thyrocytes. In particular, the induction of MMP-11 in all thyroid-derived carcinoma cell lines
studied and of MMP-13 in all but one may represent, if confirmed in other thyroid tumor-derived cell lines and
in thyroid tumor tissues, a new marker of thyrocyte transformation.</description><identifier>ISSN: 1050-7256</identifier><identifier>EISSN: 1557-9077</identifier><identifier>DOI: 10.1089/thy.2004.14.881</identifier><identifier>PMID: 15671765</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Cell Line ; Cell Line, Tumor ; Humans ; Laboratory Research Reports ; Matrix Metalloproteinases - genetics ; Matrix Metalloproteinases - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Thyroid Gland - metabolism ; Thyroid Neoplasms - enzymology ; Thyroid Neoplasms - metabolism ; Tissue Inhibitor of Metalloproteinases - metabolism</subject><ispartof>Thyroid (New York, N.Y.), 2004-11, Vol.14 (11), p.881-888</ispartof><rights>2004, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c333t-341b1653298e210e8e399e25add3607fa2d9ff880126a10abe488067818bb4e3</citedby><cites>FETCH-LOGICAL-c333t-341b1653298e210e8e399e25add3607fa2d9ff880126a10abe488067818bb4e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.liebertpub.com/doi/epdf/10.1089/thy.2004.14.881$$EPDF$$P50$$Gmaryannliebert$$H</linktopdf><linktohtml>$$Uhttps://www.liebertpub.com/doi/full/10.1089/thy.2004.14.881$$EHTML$$P50$$Gmaryannliebert$$H</linktohtml><link.rule.ids>314,776,780,3029,21702,27901,27902,55266,55278</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15671765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baldini, E.</creatorcontrib><creatorcontrib>Toller, M.</creatorcontrib><creatorcontrib>Graziano, F.M.</creatorcontrib><creatorcontrib>Russo, F.P.</creatorcontrib><creatorcontrib>Pepe, M.</creatorcontrib><creatorcontrib>Biordi, L.</creatorcontrib><creatorcontrib>Marchioni, E.</creatorcontrib><creatorcontrib>Curcio, F.</creatorcontrib><creatorcontrib>Ulisse, S.</creatorcontrib><creatorcontrib>Ambesi-Impiombato, F.S.</creatorcontrib><creatorcontrib>D’Armiento, M.</creatorcontrib><title>Expression of Matrix Metalloproteinases and Their Specific Inhibitors in Normal and Different Human Thyroid Tumor Cell Lines</title><title>Thyroid (New York, N.Y.)</title><addtitle>Thyroid</addtitle><description>In the present study we investigated, by means of zymography and reverse transcription-polymerase chain reaction
(RT-PCR), the expression of different matrix metalloproteinases (MMPs) and of the specific tissue inhibitor
of metalloproteinases [TIMPs] in human cell lines derived from normal thyrocytes (HTU5), follicular
adenoma (HTU42), and follicular (FTC-133), papillary (B-CPAP), and anaplastic (CAL-62, 8305C) thyroid carcinomas.
We demonstrated that normal thyrocytes constitutively express MMP-1, MMP-2, MMP-10, MMP-14,
and TIMP-1, TIMP-2, TIMP-3, and TIMP-4, and this pattern of expression is profoundly modified in all thyroid
tumor-derived cell lines. Analysis of the gelatinolytic activity in the different cell supernatants showed that the
expressions of MMP-2 and MMP-9 are, respectively, increased or induced in all the neoplastic cell lines, except
in CAL-62. Caseinolytic activity was found only in the supernatants of the 8305C and B-CPAP cells. Using RTPCR
analysis we detected an increased expression of MMP-1 in cell lines derived from papillary and from one
(8305C) of the two anaplastic carcinomas. MMP-13 mRNA was expressed only in the 8305C, FTC-133, and BCPAP
cells. Among stromelysins, MMP-3 mRNA could not be detected in any cell line, while MMP-10 mRNA
was expressed in all of them, although at variable levels. MMP-11 mRNA was absent in normal and follicular
adenoma derived thyrocytes and induced in all carcinoma cell types. The expression of MMP-14 (MT1-MMP)
mRNA was found significantly increased in all thyroid tumor cell lines with respect to HTU5 and HTU42 cells.
The expression of TIMP-1 and TIMP-2 mRNAs was maintained in all cell lines tested, while that of TIMP-3 was
lost in both anaplastic carcinoma cell lines and that of TIMP-4 was absent in the CAL-62. In conclusion, our
data demonstrated a differential expression of MMPs and TIMPs in different thyroid tumor cell types with respect
to normal thyrocytes. In particular, the induction of MMP-11 in all thyroid-derived carcinoma cell lines
studied and of MMP-13 in all but one may represent, if confirmed in other thyroid tumor-derived cell lines and
in thyroid tumor tissues, a new marker of thyrocyte transformation.</description><subject>Cell Line</subject><subject>Cell Line, Tumor</subject><subject>Humans</subject><subject>Laboratory Research Reports</subject><subject>Matrix Metalloproteinases - genetics</subject><subject>Matrix Metalloproteinases - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Thyroid Gland - metabolism</subject><subject>Thyroid Neoplasms - enzymology</subject><subject>Thyroid Neoplasms - metabolism</subject><subject>Tissue Inhibitor of Metalloproteinases - metabolism</subject><issn>1050-7256</issn><issn>1557-9077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkM9v2yAYhtHUaW2znXerOPXmlB824GOVZmuldDssd4TtD4XKhgyw1Ej940eWaJV24uPT876CB6GvlCwpUe1d3h2WjJB6SeulUvQDuqJNI6uWSHlRZtKQSrJGXKLrlF4IoUJJ_gld0kZIKkVzhd7Wr_sIKbngcbD42eToXvEzZDOOYR9DBudNgoSNH_B2By7iX3vonXU9fvI717kcYsLO4x8hTmb8yz04ayGCz_hxnowvuUMMruTnKUS8gnHEG-chfUYfrRkTfDmfC7T9tt6uHqvNz-9Pq_tN1XPOc8Vr2lHRcNYqYJSAAt62wBozDFwQaQ0bWmuVIpQJQ4npoC4XIRVVXVcDX6DbU235z-8ZUtaTS315hfEQ5qSFZEzUxcwC3Z3APoaUIli9j24y8aAp0UffuvjWR9-a1rr4Lombc_XcTTC882fBBahOwHFtvB8ddBDzP_D_wj9WUI0S</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Baldini, E.</creator><creator>Toller, M.</creator><creator>Graziano, F.M.</creator><creator>Russo, F.P.</creator><creator>Pepe, M.</creator><creator>Biordi, L.</creator><creator>Marchioni, E.</creator><creator>Curcio, F.</creator><creator>Ulisse, S.</creator><creator>Ambesi-Impiombato, F.S.</creator><creator>D’Armiento, M.</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Expression of Matrix Metalloproteinases and Their Specific Inhibitors in Normal and Different Human Thyroid Tumor Cell Lines</title><author>Baldini, E. ; Toller, M. ; Graziano, F.M. ; Russo, F.P. ; Pepe, M. ; Biordi, L. ; Marchioni, E. ; Curcio, F. ; Ulisse, S. ; Ambesi-Impiombato, F.S. ; D’Armiento, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-341b1653298e210e8e399e25add3607fa2d9ff880126a10abe488067818bb4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Cell Line</topic><topic>Cell Line, Tumor</topic><topic>Humans</topic><topic>Laboratory Research Reports</topic><topic>Matrix Metalloproteinases - genetics</topic><topic>Matrix Metalloproteinases - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Thyroid Gland - metabolism</topic><topic>Thyroid Neoplasms - enzymology</topic><topic>Thyroid Neoplasms - metabolism</topic><topic>Tissue Inhibitor of Metalloproteinases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baldini, E.</creatorcontrib><creatorcontrib>Toller, M.</creatorcontrib><creatorcontrib>Graziano, F.M.</creatorcontrib><creatorcontrib>Russo, F.P.</creatorcontrib><creatorcontrib>Pepe, M.</creatorcontrib><creatorcontrib>Biordi, L.</creatorcontrib><creatorcontrib>Marchioni, E.</creatorcontrib><creatorcontrib>Curcio, F.</creatorcontrib><creatorcontrib>Ulisse, S.</creatorcontrib><creatorcontrib>Ambesi-Impiombato, F.S.</creatorcontrib><creatorcontrib>D’Armiento, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Thyroid (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baldini, E.</au><au>Toller, M.</au><au>Graziano, F.M.</au><au>Russo, F.P.</au><au>Pepe, M.</au><au>Biordi, L.</au><au>Marchioni, E.</au><au>Curcio, F.</au><au>Ulisse, S.</au><au>Ambesi-Impiombato, F.S.</au><au>D’Armiento, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Matrix Metalloproteinases and Their Specific Inhibitors in Normal and Different Human Thyroid Tumor Cell Lines</atitle><jtitle>Thyroid (New York, N.Y.)</jtitle><addtitle>Thyroid</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>14</volume><issue>11</issue><spage>881</spage><epage>888</epage><pages>881-888</pages><issn>1050-7256</issn><eissn>1557-9077</eissn><abstract>In the present study we investigated, by means of zymography and reverse transcription-polymerase chain reaction
(RT-PCR), the expression of different matrix metalloproteinases (MMPs) and of the specific tissue inhibitor
of metalloproteinases [TIMPs] in human cell lines derived from normal thyrocytes (HTU5), follicular
adenoma (HTU42), and follicular (FTC-133), papillary (B-CPAP), and anaplastic (CAL-62, 8305C) thyroid carcinomas.
We demonstrated that normal thyrocytes constitutively express MMP-1, MMP-2, MMP-10, MMP-14,
and TIMP-1, TIMP-2, TIMP-3, and TIMP-4, and this pattern of expression is profoundly modified in all thyroid
tumor-derived cell lines. Analysis of the gelatinolytic activity in the different cell supernatants showed that the
expressions of MMP-2 and MMP-9 are, respectively, increased or induced in all the neoplastic cell lines, except
in CAL-62. Caseinolytic activity was found only in the supernatants of the 8305C and B-CPAP cells. Using RTPCR
analysis we detected an increased expression of MMP-1 in cell lines derived from papillary and from one
(8305C) of the two anaplastic carcinomas. MMP-13 mRNA was expressed only in the 8305C, FTC-133, and BCPAP
cells. Among stromelysins, MMP-3 mRNA could not be detected in any cell line, while MMP-10 mRNA
was expressed in all of them, although at variable levels. MMP-11 mRNA was absent in normal and follicular
adenoma derived thyrocytes and induced in all carcinoma cell types. The expression of MMP-14 (MT1-MMP)
mRNA was found significantly increased in all thyroid tumor cell lines with respect to HTU5 and HTU42 cells.
The expression of TIMP-1 and TIMP-2 mRNAs was maintained in all cell lines tested, while that of TIMP-3 was
lost in both anaplastic carcinoma cell lines and that of TIMP-4 was absent in the CAL-62. In conclusion, our
data demonstrated a differential expression of MMPs and TIMPs in different thyroid tumor cell types with respect
to normal thyrocytes. In particular, the induction of MMP-11 in all thyroid-derived carcinoma cell lines
studied and of MMP-13 in all but one may represent, if confirmed in other thyroid tumor-derived cell lines and
in thyroid tumor tissues, a new marker of thyrocyte transformation.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>15671765</pmid><doi>10.1089/thy.2004.14.881</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1050-7256 |
| ispartof | Thyroid (New York, N.Y.), 2004-11, Vol.14 (11), p.881-888 |
| issn | 1050-7256 1557-9077 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67226487 |
| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Cell Line Cell Line, Tumor Humans Laboratory Research Reports Matrix Metalloproteinases - genetics Matrix Metalloproteinases - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Thyroid Gland - metabolism Thyroid Neoplasms - enzymology Thyroid Neoplasms - metabolism Tissue Inhibitor of Metalloproteinases - metabolism |
| title | Expression of Matrix Metalloproteinases and Their Specific Inhibitors in Normal and Different Human Thyroid Tumor Cell Lines |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T20%3A34%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20Matrix%20Metalloproteinases%20and%20Their%20Specific%20Inhibitors%20in%20Normal%20and%20Different%20Human%20Thyroid%20Tumor%20Cell%20Lines&rft.jtitle=Thyroid%20(New%20York,%20N.Y.)&rft.au=Baldini,%20E.&rft.date=2004-11-01&rft.volume=14&rft.issue=11&rft.spage=881&rft.epage=888&rft.pages=881-888&rft.issn=1050-7256&rft.eissn=1557-9077&rft_id=info:doi/10.1089/thy.2004.14.881&rft_dat=%3Cproquest_cross%3E67226487%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67226487&rft_id=info:pmid/15671765&rfr_iscdi=true |