Modulation of Kir4.1 and Kir4.1–Kir5.1 channels by small changes in cell volume

The K+ channels Kir4.1 and Kir4.1–Kir5.1 are expressed in the glial cells of the CNS and are involved in regulation of the K+ homeostasis. Several studies have shown that Kir4.1 channels are co-localized with aquaporins (AQP4) in the glial endfeet, and a putative functional coupling between the Kir...

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Veröffentlicht in:Neuroscience letters 2009-06, Vol.457 (2), p.80-84
Hauptverfasser: Soe, Rikke, MacAulay, Nanna, Klaerke, Dan Arne
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creator Soe, Rikke
MacAulay, Nanna
Klaerke, Dan Arne
description The K+ channels Kir4.1 and Kir4.1–Kir5.1 are expressed in the glial cells of the CNS and are involved in regulation of the K+ homeostasis. Several studies have shown that Kir4.1 channels are co-localized with aquaporins (AQP4) in the glial endfeet, and a putative functional coupling between the Kir channels and aquaporins is therefore debated. To test a possible volume-sensitivity of the Kir channels, the Kir4.1 or Kir4.1–Kir5.1 channels were expressed in Xenopus oocytes with or without co-expression of aquaporins and subsequently exposed to cell volume alterations. Our results show an increase in Kir4.1 and Kir4.1–Kir5.1 currents upon swelling of the oocytes and a reduction in the current when the oocytes were shrunk. The volume-dependent changes in channel activity were not due to changes in the kinetics of the channels. These findings implicate a putative functional interaction between the Kir channels and aquaporins via small, fast cell volume changes in the glial cells.
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source MEDLINE; Elsevier ScienceDirect Journals Complete
subjects Animals
AQP4
Aquaporin 1 - metabolism
Aquaporin 4 - metabolism
Biological and medical sciences
Cell Size
Fundamental and applied biological sciences. Psychology
Glial cell swelling
Humans
K+ channels
Kir5.1 Channel
Membrane Potentials - physiology
Oocytes - cytology
Oocytes - metabolism
Patch-Clamp Techniques
Potassium Channels, Inwardly Rectifying - metabolism
Rats
Regulation
Vertebrates: nervous system and sense organs
Volume changes
Xenopus laevis
title Modulation of Kir4.1 and Kir4.1–Kir5.1 channels by small changes in cell volume
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