A 78 kDa glucose-regulated protein gene of Spirometra erinacei plerocercoid induced by chemical and physiological stresses

To adapt to different environmental conditions between poikilothermic and homeothermic hosts, the plerocercoid of Spirometra erinacei (sparganum) might express a variety of biologically active molecules. We have identified a 78 kDa glucose-regulated protein of the sparganum (SpGrp78) by differential...

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Veröffentlicht in:Parasitology 2004-12, Vol.129 (6), p.713-721
Hauptverfasser: YUN, D.-H., BAE, Y.-A., CHUNG, J.-Y., KANG, S.-Y., KANG, I., SOHN, W.-M., CHO, S.-H., KIM, T.-S., CHO, S.-Y., KONG, Y.
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container_issue 6
container_start_page 713
container_title Parasitology
container_volume 129
creator YUN, D.-H.
BAE, Y.-A.
CHUNG, J.-Y.
KANG, S.-Y.
KANG, I.
SOHN, W.-M.
CHO, S.-H.
KIM, T.-S.
CHO, S.-Y.
KONG, Y.
description To adapt to different environmental conditions between poikilothermic and homeothermic hosts, the plerocercoid of Spirometra erinacei (sparganum) might express a variety of biologically active molecules. We have identified a 78 kDa glucose-regulated protein of the sparganum (SpGrp78) by differential display of mRNA, employing RNAs each from sparganum adjusted at 9 °C and 37 °C. A full-length cDNA of 2148 bp encodes for a protein of 651 amino acids with a predicted molecular mass of 71 610 Da and shares molecular characteristics with heat-shock protein 70, including a putative ATP binding site, signal peptide cleavage site and endoplasmic reticulum retention signal. Phylogenetic analysis revealed that SpGrp78 was mostly related to those of Echinococcus multilocularis and E. granulosus. Expression of SpGrp78 mRNA increased approximately 7-fold by inhibition of glycosylation by tunicamycin, 2-fold by temperature-shift from 9 °C to 37 °C and slightly by pH-shift to 4·0 or 5·5. These results suggested that induction of SpGrp78 mRNA is related to the functional role of SpGrp78 as a molecular chaperone when the parasite adapts to a new host environment.
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Models ; Glucose ; glucose-regulated protein ; Glycosylation ; Heat shock proteins ; heat-shock protein ; Heat-Shock Proteins - biosynthesis ; Heat-Shock Proteins - physiology ; Helminth Proteins - biosynthesis ; Helminth Proteins - physiology ; Host-Parasite Interactions ; Infections ; Invertebrates ; Mammals ; Medicine ; Molecular chains ; molecular chaperones ; Molecular Chaperones - biosynthesis ; Molecular Chaperones - physiology ; Molecular Sequence Data ; mRNA ; Organic chemistry ; Parasites ; Parasitic diseases ; Parasitology ; Phylogeny ; Physiology ; Proteins ; Research centers ; RNA, Messenger ; Sequence Alignment ; Sequence Homology, Amino Acid ; Sparganum - drug effects ; Sparganum - genetics ; Sparganum - physiology ; Species Specificity ; Spirometra ; Spirometra erinacei plerocercoid (sparganum) ; Stress (physiology) ; stress protein ; Tropical diseases ; Tunicamycin</subject><ispartof>Parasitology, 2004-12, Vol.129 (6), p.713-721</ispartof><rights>2004 Cambridge University Press</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-fd4443a41b90bed9a0e699fca3a837016a9374330d602ccaf8ca419806b096183</citedby><cites>FETCH-LOGICAL-c439t-fd4443a41b90bed9a0e699fca3a837016a9374330d602ccaf8ca419806b096183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182004006158/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,315,782,786,27931,27932,55635</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=16318300$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15648694$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YUN, D.-H.</creatorcontrib><creatorcontrib>BAE, Y.-A.</creatorcontrib><creatorcontrib>CHUNG, J.-Y.</creatorcontrib><creatorcontrib>KANG, S.-Y.</creatorcontrib><creatorcontrib>KANG, I.</creatorcontrib><creatorcontrib>SOHN, W.-M.</creatorcontrib><creatorcontrib>CHO, S.-H.</creatorcontrib><creatorcontrib>KIM, T.-S.</creatorcontrib><creatorcontrib>CHO, S.-Y.</creatorcontrib><creatorcontrib>KONG, Y.</creatorcontrib><title>A 78 kDa glucose-regulated protein gene of Spirometra erinacei plerocercoid induced by chemical and physiological stresses</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>To adapt to different environmental conditions between poikilothermic and homeothermic hosts, the plerocercoid of Spirometra erinacei (sparganum) might express a variety of biologically active molecules. 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Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gene Expression Regulation - physiology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. 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These results suggested that induction of SpGrp78 mRNA is related to the functional role of SpGrp78 as a molecular chaperone when the parasite adapts to a new host environment.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>15648694</pmid><doi>10.1017/S0031182004006158</doi><tpages>9</tpages></addata></record>
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identifier ISSN: 0031-1820
ispartof Parasitology, 2004-12, Vol.129 (6), p.713-721
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recordid cdi_proquest_miscellaneous_67222814
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subjects Adaptation, Physiological
Amino Acid Sequence
Amino acids
Animals
Binding sites
Biological activity
Biological and medical sciences
Biomedical research
Cloning
Deoxyribonucleic acid
Differential display
differential display PCR
DNA
Endoplasmic reticulum
Environmental conditions
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation - drug effects
Gene Expression Regulation - physiology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Glucose
glucose-regulated protein
Glycosylation
Heat shock proteins
heat-shock protein
Heat-Shock Proteins - biosynthesis
Heat-Shock Proteins - physiology
Helminth Proteins - biosynthesis
Helminth Proteins - physiology
Host-Parasite Interactions
Infections
Invertebrates
Mammals
Medicine
Molecular chains
molecular chaperones
Molecular Chaperones - biosynthesis
Molecular Chaperones - physiology
Molecular Sequence Data
mRNA
Organic chemistry
Parasites
Parasitic diseases
Parasitology
Phylogeny
Physiology
Proteins
Research centers
RNA, Messenger
Sequence Alignment
Sequence Homology, Amino Acid
Sparganum - drug effects
Sparganum - genetics
Sparganum - physiology
Species Specificity
Spirometra
Spirometra erinacei plerocercoid (sparganum)
Stress (physiology)
stress protein
Tropical diseases
Tunicamycin
title A 78 kDa glucose-regulated protein gene of Spirometra erinacei plerocercoid induced by chemical and physiological stresses
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