Differential scanning calorimetry of blood plasma for clinical diagnosis and monitoring
Differential scanning calorimetry (DSC) provides a useful method to study the unfractionated plasma proteome. Plasma from healthy individuals yields a reproducible signature thermogram which results from the weighted sum of the thermal denaturation of the most abundant plasma proteins. Further inves...
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| Veröffentlicht in: | Experimental and molecular pathology 2009-06, Vol.86 (3), p.186-191 |
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| container_title | Experimental and molecular pathology |
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| creator | Garbett, Nichola C. Mekmaysy, Chongkham S. Helm, C. William Jenson, A. Bennett Chaires, Jonathan B. |
| description | Differential scanning calorimetry (DSC) provides a useful method to study the unfractionated plasma proteome. Plasma from healthy individuals yields a reproducible signature thermogram which results from the weighted sum of the thermal denaturation of the most abundant plasma proteins. Further investigation of the thermogram for healthy individuals showed it to be sensitive to ethnicity and gender. DSC analysis of plasma from diseased individuals revealed significant changes in the thermogram which are suggested to result not from changes in the concentration of the major plasma proteins but from interactions of small molecules or peptides with these proteins. Closer examination of the diseased thermograms showed a thermogram characteristic of each disease. For cervical cancer, the DSC method yields a progressively shifted thermogram as the disease advances from pre-invasive conditions to late stage cancer. Our application of the DSC method has provided a potential tool for the early diagnosis, monitoring and screening of cancer patients. |
| doi_str_mv | 10.1016/j.yexmp.2008.12.001 |
| format | Article |
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Closer examination of the diseased thermograms showed a thermogram characteristic of each disease. For cervical cancer, the DSC method yields a progressively shifted thermogram as the disease advances from pre-invasive conditions to late stage cancer. Our application of the DSC method has provided a potential tool for the early diagnosis, monitoring and screening of cancer patients.</description><subject>Blood Proteins - chemistry</subject><subject>Calorimetry, Differential Scanning - methods</subject><subject>Female</subject><subject>Humans</subject><subject>Ligands</subject><subject>Neoplasm Proteins - blood</subject><subject>Neoplasm Staging - methods</subject><subject>Neoplasms - blood</subject><subject>Neoplasms - diagnosis</subject><subject>Plasma - chemistry</subject><subject>Protein Binding</subject><subject>Proteomics - methods</subject><subject>Uterine Cervical Neoplasms - blood</subject><subject>Uterine Cervical Neoplasms - diagnosis</subject><issn>0014-4800</issn><issn>1096-0945</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVpaLZJf0Gh6JSbXY0sa61DDiUfbSHQS0uOQh_jRYstbSRvyf77KN2F3HoaGJ73HeYh5DOwFhjIr9v2gM_zruWMDS3wljF4R1bAlGyYEv17sqob0YiBsXPysZQtY0wx4B_IOSgQchBqRR5vwzhixrgEM9HiTIwhbqgzU8phxiUfaBqpnVLydDeZMhs6pkzdFGKoEPXBbGIqoVATPZ1TDEsNxs0lORvNVPDTaV6QP_d3v29-NA-_vv-8-fbQuK5fL02HayvWHXcKwA3OeGOMc9ZK4S32xvcKHR97sOBUP1irJICUVvhKCyZ5d0Gujr27nJ72WBY9h-JwmkzEtC9arjnnPe8q2B1Bl1MpGUe9qw-afNDA9KtPvdX_fOpXnxq4rvZq6supfm9n9G-Zk8AKXB8BrE_-DZh1cQGjQx8yukX7FP574AWNw4nh</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Garbett, Nichola C.</creator><creator>Mekmaysy, Chongkham S.</creator><creator>Helm, C. 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Bennett</au><au>Chaires, Jonathan B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential scanning calorimetry of blood plasma for clinical diagnosis and monitoring</atitle><jtitle>Experimental and molecular pathology</jtitle><addtitle>Exp Mol Pathol</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>86</volume><issue>3</issue><spage>186</spage><epage>191</epage><pages>186-191</pages><issn>0014-4800</issn><eissn>1096-0945</eissn><abstract>Differential scanning calorimetry (DSC) provides a useful method to study the unfractionated plasma proteome. Plasma from healthy individuals yields a reproducible signature thermogram which results from the weighted sum of the thermal denaturation of the most abundant plasma proteins. Further investigation of the thermogram for healthy individuals showed it to be sensitive to ethnicity and gender. DSC analysis of plasma from diseased individuals revealed significant changes in the thermogram which are suggested to result not from changes in the concentration of the major plasma proteins but from interactions of small molecules or peptides with these proteins. Closer examination of the diseased thermograms showed a thermogram characteristic of each disease. For cervical cancer, the DSC method yields a progressively shifted thermogram as the disease advances from pre-invasive conditions to late stage cancer. Our application of the DSC method has provided a potential tool for the early diagnosis, monitoring and screening of cancer patients.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>19146849</pmid><doi>10.1016/j.yexmp.2008.12.001</doi><tpages>6</tpages></addata></record> |
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| ispartof | Experimental and molecular pathology, 2009-06, Vol.86 (3), p.186-191 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Blood Proteins - chemistry Calorimetry, Differential Scanning - methods Female Humans Ligands Neoplasm Proteins - blood Neoplasm Staging - methods Neoplasms - blood Neoplasms - diagnosis Plasma - chemistry Protein Binding Proteomics - methods Uterine Cervical Neoplasms - blood Uterine Cervical Neoplasms - diagnosis |
| title | Differential scanning calorimetry of blood plasma for clinical diagnosis and monitoring |
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