Identification and expression analysis of miRNAs during batch culture of HEK-293 cells

MicroRNAs (miRNAs) are small non-coding RNAs of about 20–24 nucleotides in length. They regulate gene expression negatively and have been implicated in a wide variety of biological processes. To identify potential miRNAs that may influence the growth and proliferation of mammalian cells cultured in...

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Veröffentlicht in:	Journal of biotechnology 2009-03, Vol.140 (3-4), p.149-155
Hauptverfasser:	Koh, Thong-Chuan, Lee, Yih-Yean, Chang, Siow-Qi, Nissom, Peter Morin
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis - genetics Apoptosis - physiology Batch cell culture Biological and medical sciences Bioreactors Biotechnology Cell Differentiation - genetics Cell Differentiation - physiology Cell Line CHO Fundamental and applied biological sciences. Psychology HEK293 Humans microRNA (miRNA) MicroRNAs - genetics MicroRNAs - physiology Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction
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