Antimicrobial susceptibility testing of lactic acid bacteria and bifidobacteria by broth microdilution method and Etest
We applied two methods of broth microdilution and Etest for measuring minimal inhibition concentration (MIC) of lactic acid bacteria and bifidobacteria for 15 antimicrobial agents to compare the feasibility, reproducibility, and equivalence of the two methods. Both methods were originally described...
Gespeichert in:
| Veröffentlicht in: | International journal of food microbiology 2009-06, Vol.132 (1), p.54-58 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 58 |
|---|---|
| container_issue | 1 |
| container_start_page | 54 |
| container_title | International journal of food microbiology |
| container_volume | 132 |
| creator | Kushiro, Akira Chervaux, Christian Cools-Portier, Stephanie Perony, Audrey Legrain-Raspaud, Sophie Obis, David Onoue, Masaharu van de Moer, Ariane |
| description | We applied two methods of broth microdilution and Etest for measuring minimal inhibition concentration (MIC) of lactic acid bacteria and bifidobacteria for 15 antimicrobial agents to compare the feasibility, reproducibility, and equivalence of the two methods. Both methods were originally described by the European projects PROSAFE and ACE-ART. In 84% combinations of strains and antimicrobial agents MIC differences between the two methods were within one Log
2 dilution. In the case of rifampicin the difference between the two methods was more than ten fold. We further determined MICs of 70 strains (14 strains of
Lactobacillus delbrueckii ssp.
bulgaricus, 16 strains of
Lactococcus lactis, 30 strains of
Streptococcus thermophilus, and 10 strains of
Bifidobacterium longum) by the broth microdilution method. In most cases, MIC distributions were uni-modal and within 5 Log
2 dilutions except for the MIC distribution of
L. lactis to the aminoglycoside group which was broader. These data are a good basis for improving knowledge of antimicrobial susceptibility of lactic acid bacteria and bifidobacteria, and can be used to revise tentative epidemiological cut-off values. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2009.03.012 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67217652</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S016816050900169X</els_id><sourcerecordid>20601735</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-d19d68a330ec6b723b9e31f7a82967d4a17dc3711f185bcb571ec887a3412e0c3</originalsourceid><addsrcrecordid>eNqNkUtv1DAUhSMEotPCXwCzoLsEXzuxk2U1Kg-pEgvo2vIr7R1l4sF2QPPv8TxU2MHK9tV3z7nXp6reAW2AgviwaXAzhuC2aGNoGKVDQ3lDgT2rVtDLoeatoM-rVWH7GgTtLqrLlDaU0o5z-rK6gIGLAspV9etmznjUMagnkpZk_S6jwQnznmSfMs4PJIxk0jajJdqiI6bcfURN9FweOKILTyWzJyaG_EiOog6nJWOYydbnx-CODbcH1VfVi1FPyb8-n1fV_cfb7-vP9d3XT1_WN3e17ZjItYPBiV6Xqb0VRjJuBs9hlLpng5Cu1SCd5RJghL4z1nQSvO17qXkLzFPLr6rrk-4uhh9LMVZbLCtOk559WJISkoEUHfsnyKigIHlXwOEElvVSin5Uu4hbHfcKqDrEozbqr3jUIR5FuSrxlN43Z5PFbL3703nOowDvz4BOVk9j1LPF9MQx6Fo6DG3h3p64UQelH2Jh7r8xCrz4cyrkgVifCF9-9yf6qJJFP1vvMHqblQv4HwP_BmQUvzs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20601735</pqid></control><display><type>article</type><title>Antimicrobial susceptibility testing of lactic acid bacteria and bifidobacteria by broth microdilution method and Etest</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Kushiro, Akira ; Chervaux, Christian ; Cools-Portier, Stephanie ; Perony, Audrey ; Legrain-Raspaud, Sophie ; Obis, David ; Onoue, Masaharu ; van de Moer, Ariane</creator><creatorcontrib>Kushiro, Akira ; Chervaux, Christian ; Cools-Portier, Stephanie ; Perony, Audrey ; Legrain-Raspaud, Sophie ; Obis, David ; Onoue, Masaharu ; van de Moer, Ariane</creatorcontrib><description>We applied two methods of broth microdilution and Etest for measuring minimal inhibition concentration (MIC) of lactic acid bacteria and bifidobacteria for 15 antimicrobial agents to compare the feasibility, reproducibility, and equivalence of the two methods. Both methods were originally described by the European projects PROSAFE and ACE-ART. In 84% combinations of strains and antimicrobial agents MIC differences between the two methods were within one Log
2 dilution. In the case of rifampicin the difference between the two methods was more than ten fold. We further determined MICs of 70 strains (14 strains of
Lactobacillus delbrueckii ssp.
bulgaricus, 16 strains of
Lactococcus lactis, 30 strains of
Streptococcus thermophilus, and 10 strains of
Bifidobacterium longum) by the broth microdilution method. In most cases, MIC distributions were uni-modal and within 5 Log
2 dilutions except for the MIC distribution of
L. lactis to the aminoglycoside group which was broader. These data are a good basis for improving knowledge of antimicrobial susceptibility of lactic acid bacteria and bifidobacteria, and can be used to revise tentative epidemiological cut-off values.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2009.03.012</identifier><identifier>PMID: 19361877</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Anti-Bacterial Agents - pharmacology ; antibacterial properties ; antibiotic resistance ; antibiotics ; Bifidobacteria ; Bifidobacterium ; Bifidobacterium - drug effects ; Bifidobacterium - growth & development ; Bifidobacterium longum ; Biological and medical sciences ; Broth microdilution ; culture media ; Etest ; Food industries ; Food microbiology ; Fundamental and applied biological sciences. Psychology ; Gram-positive bacteria ; Lactic acid bacteria ; Lactobacillaceae - drug effects ; Lactobacillaceae - growth & development ; Lactobacillus delbrueckii ; Lactobacillus delbrueckii subsp. bulgaricus ; Lactococcus lactis ; Microbial Sensitivity Tests - methods ; Minimal inhibition concentration (MIC) ; minimum inhibitory concentration ; probiotics ; repeatability ; strains ; Streptococcus thermophilus</subject><ispartof>International journal of food microbiology, 2009-06, Vol.132 (1), p.54-58</ispartof><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-d19d68a330ec6b723b9e31f7a82967d4a17dc3711f185bcb571ec887a3412e0c3</citedby><cites>FETCH-LOGICAL-c526t-d19d68a330ec6b723b9e31f7a82967d4a17dc3711f185bcb571ec887a3412e0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2009.03.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21540994$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19361877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kushiro, Akira</creatorcontrib><creatorcontrib>Chervaux, Christian</creatorcontrib><creatorcontrib>Cools-Portier, Stephanie</creatorcontrib><creatorcontrib>Perony, Audrey</creatorcontrib><creatorcontrib>Legrain-Raspaud, Sophie</creatorcontrib><creatorcontrib>Obis, David</creatorcontrib><creatorcontrib>Onoue, Masaharu</creatorcontrib><creatorcontrib>van de Moer, Ariane</creatorcontrib><title>Antimicrobial susceptibility testing of lactic acid bacteria and bifidobacteria by broth microdilution method and Etest</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>We applied two methods of broth microdilution and Etest for measuring minimal inhibition concentration (MIC) of lactic acid bacteria and bifidobacteria for 15 antimicrobial agents to compare the feasibility, reproducibility, and equivalence of the two methods. Both methods were originally described by the European projects PROSAFE and ACE-ART. In 84% combinations of strains and antimicrobial agents MIC differences between the two methods were within one Log
2 dilution. In the case of rifampicin the difference between the two methods was more than ten fold. We further determined MICs of 70 strains (14 strains of
Lactobacillus delbrueckii ssp.
bulgaricus, 16 strains of
Lactococcus lactis, 30 strains of
Streptococcus thermophilus, and 10 strains of
Bifidobacterium longum) by the broth microdilution method. In most cases, MIC distributions were uni-modal and within 5 Log
2 dilutions except for the MIC distribution of
L. lactis to the aminoglycoside group which was broader. These data are a good basis for improving knowledge of antimicrobial susceptibility of lactic acid bacteria and bifidobacteria, and can be used to revise tentative epidemiological cut-off values.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibacterial properties</subject><subject>antibiotic resistance</subject><subject>antibiotics</subject><subject>Bifidobacteria</subject><subject>Bifidobacterium</subject><subject>Bifidobacterium - drug effects</subject><subject>Bifidobacterium - growth & development</subject><subject>Bifidobacterium longum</subject><subject>Biological and medical sciences</subject><subject>Broth microdilution</subject><subject>culture media</subject><subject>Etest</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gram-positive bacteria</subject><subject>Lactic acid bacteria</subject><subject>Lactobacillaceae - drug effects</subject><subject>Lactobacillaceae - growth & development</subject><subject>Lactobacillus delbrueckii</subject><subject>Lactobacillus delbrueckii subsp. bulgaricus</subject><subject>Lactococcus lactis</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Minimal inhibition concentration (MIC)</subject><subject>minimum inhibitory concentration</subject><subject>probiotics</subject><subject>repeatability</subject><subject>strains</subject><subject>Streptococcus thermophilus</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAUhSMEotPCXwCzoLsEXzuxk2U1Kg-pEgvo2vIr7R1l4sF2QPPv8TxU2MHK9tV3z7nXp6reAW2AgviwaXAzhuC2aGNoGKVDQ3lDgT2rVtDLoeatoM-rVWH7GgTtLqrLlDaU0o5z-rK6gIGLAspV9etmznjUMagnkpZk_S6jwQnznmSfMs4PJIxk0jajJdqiI6bcfURN9FweOKILTyWzJyaG_EiOog6nJWOYydbnx-CODbcH1VfVi1FPyb8-n1fV_cfb7-vP9d3XT1_WN3e17ZjItYPBiV6Xqb0VRjJuBs9hlLpng5Cu1SCd5RJghL4z1nQSvO17qXkLzFPLr6rrk-4uhh9LMVZbLCtOk559WJISkoEUHfsnyKigIHlXwOEElvVSin5Uu4hbHfcKqDrEozbqr3jUIR5FuSrxlN43Z5PFbL3703nOowDvz4BOVk9j1LPF9MQx6Fo6DG3h3p64UQelH2Jh7r8xCrz4cyrkgVifCF9-9yf6qJJFP1vvMHqblQv4HwP_BmQUvzs</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Kushiro, Akira</creator><creator>Chervaux, Christian</creator><creator>Cools-Portier, Stephanie</creator><creator>Perony, Audrey</creator><creator>Legrain-Raspaud, Sophie</creator><creator>Obis, David</creator><creator>Onoue, Masaharu</creator><creator>van de Moer, Ariane</creator><general>Elsevier B.V</general><general>[Amsterdam; New York, NY]: Elsevier Science</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>Antimicrobial susceptibility testing of lactic acid bacteria and bifidobacteria by broth microdilution method and Etest</title><author>Kushiro, Akira ; Chervaux, Christian ; Cools-Portier, Stephanie ; Perony, Audrey ; Legrain-Raspaud, Sophie ; Obis, David ; Onoue, Masaharu ; van de Moer, Ariane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-d19d68a330ec6b723b9e31f7a82967d4a17dc3711f185bcb571ec887a3412e0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibacterial properties</topic><topic>antibiotic resistance</topic><topic>antibiotics</topic><topic>Bifidobacteria</topic><topic>Bifidobacterium</topic><topic>Bifidobacterium - drug effects</topic><topic>Bifidobacterium - growth & development</topic><topic>Bifidobacterium longum</topic><topic>Biological and medical sciences</topic><topic>Broth microdilution</topic><topic>culture media</topic><topic>Etest</topic><topic>Food industries</topic><topic>Food microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gram-positive bacteria</topic><topic>Lactic acid bacteria</topic><topic>Lactobacillaceae - drug effects</topic><topic>Lactobacillaceae - growth & development</topic><topic>Lactobacillus delbrueckii</topic><topic>Lactobacillus delbrueckii subsp. bulgaricus</topic><topic>Lactococcus lactis</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Minimal inhibition concentration (MIC)</topic><topic>minimum inhibitory concentration</topic><topic>probiotics</topic><topic>repeatability</topic><topic>strains</topic><topic>Streptococcus thermophilus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kushiro, Akira</creatorcontrib><creatorcontrib>Chervaux, Christian</creatorcontrib><creatorcontrib>Cools-Portier, Stephanie</creatorcontrib><creatorcontrib>Perony, Audrey</creatorcontrib><creatorcontrib>Legrain-Raspaud, Sophie</creatorcontrib><creatorcontrib>Obis, David</creatorcontrib><creatorcontrib>Onoue, Masaharu</creatorcontrib><creatorcontrib>van de Moer, Ariane</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kushiro, Akira</au><au>Chervaux, Christian</au><au>Cools-Portier, Stephanie</au><au>Perony, Audrey</au><au>Legrain-Raspaud, Sophie</au><au>Obis, David</au><au>Onoue, Masaharu</au><au>van de Moer, Ariane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antimicrobial susceptibility testing of lactic acid bacteria and bifidobacteria by broth microdilution method and Etest</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>132</volume><issue>1</issue><spage>54</spage><epage>58</epage><pages>54-58</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>We applied two methods of broth microdilution and Etest for measuring minimal inhibition concentration (MIC) of lactic acid bacteria and bifidobacteria for 15 antimicrobial agents to compare the feasibility, reproducibility, and equivalence of the two methods. Both methods were originally described by the European projects PROSAFE and ACE-ART. In 84% combinations of strains and antimicrobial agents MIC differences between the two methods were within one Log
2 dilution. In the case of rifampicin the difference between the two methods was more than ten fold. We further determined MICs of 70 strains (14 strains of
Lactobacillus delbrueckii ssp.
bulgaricus, 16 strains of
Lactococcus lactis, 30 strains of
Streptococcus thermophilus, and 10 strains of
Bifidobacterium longum) by the broth microdilution method. In most cases, MIC distributions were uni-modal and within 5 Log
2 dilutions except for the MIC distribution of
L. lactis to the aminoglycoside group which was broader. These data are a good basis for improving knowledge of antimicrobial susceptibility of lactic acid bacteria and bifidobacteria, and can be used to revise tentative epidemiological cut-off values.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19361877</pmid><doi>10.1016/j.ijfoodmicro.2009.03.012</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1605 |
| ispartof | International journal of food microbiology, 2009-06, Vol.132 (1), p.54-58 |
| issn | 0168-1605 1879-3460 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67217652 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Anti-Bacterial Agents - pharmacology antibacterial properties antibiotic resistance antibiotics Bifidobacteria Bifidobacterium Bifidobacterium - drug effects Bifidobacterium - growth & development Bifidobacterium longum Biological and medical sciences Broth microdilution culture media Etest Food industries Food microbiology Fundamental and applied biological sciences. Psychology Gram-positive bacteria Lactic acid bacteria Lactobacillaceae - drug effects Lactobacillaceae - growth & development Lactobacillus delbrueckii Lactobacillus delbrueckii subsp. bulgaricus Lactococcus lactis Microbial Sensitivity Tests - methods Minimal inhibition concentration (MIC) minimum inhibitory concentration probiotics repeatability strains Streptococcus thermophilus |
| title | Antimicrobial susceptibility testing of lactic acid bacteria and bifidobacteria by broth microdilution method and Etest |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T11%3A08%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antimicrobial%20susceptibility%20testing%20of%20lactic%20acid%20bacteria%20and%20bifidobacteria%20by%20broth%20microdilution%20method%20and%20Etest&rft.jtitle=International%20journal%20of%20food%20microbiology&rft.au=Kushiro,%20Akira&rft.date=2009-06-01&rft.volume=132&rft.issue=1&rft.spage=54&rft.epage=58&rft.pages=54-58&rft.issn=0168-1605&rft.eissn=1879-3460&rft.coden=IJFMDD&rft_id=info:doi/10.1016/j.ijfoodmicro.2009.03.012&rft_dat=%3Cproquest_cross%3E20601735%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20601735&rft_id=info:pmid/19361877&rft_els_id=S016816050900169X&rfr_iscdi=true |