Obese Women Exhibit Differences in Ovarian Metabolites, Hormones, and Gene Expression Compared with Moderate-Weight Women
Context: Obese women experience longer times to conception, even if they are young and cycling regularly, which is suggestive of alterations in ovarian function during the periconceptual period. Objective: This study sought to determine whether there are alterations in the preovulatory follicular en...
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| creator | Robker, Rebecca L. Akison, Lisa K. Bennett, Brenton D. Thrupp, Penny N. Chura, Lindsay R. Russell, Darryl L. Lane, Michelle Norman, Robert J. |
| description | Context: Obese women experience longer times to conception, even if they are young and cycling regularly, which is suggestive of alterations in ovarian function during the periconceptual period.
Objective: This study sought to determine whether there are alterations in the preovulatory follicular environment that are likely to influence oocyte developmental competence.
Design, Setting, and Participants: Women attending a private infertility clinic were categorized into body mass index (BMI) groups of moderate (n = 33; BMI 20–24.9 kg/m2), overweight (n = 31; BMI 25–29.9 kg/m2), and obese (n =32; BMI ≥30 kg/m2).
Intervention: For each patient, follicular fluid was recovered from single follicles at oocyte retrieval, granulosa cells were pooled from multiple follicular aspirates and cumulus cells were pooled after separation from the oocytes.
Main Outcome Measures: Follicle fluid was assayed for hormones and metabolites. Granulosa and cumulus cells were analyzed for mRNA expression of insulin signaling components (IRS-2 and Glut4), glucose-regulated genes (ChREBP, ACC, and FAS) and insulin-regulated genes (SREBP-1, CD36, and SR-BI) associated with obesity/insulin resistance.
Results: Increasing BMI was associated with increased follicular fluid insulin (P < 0.001), lactate (P = 0.01), triglycerides (P = 0.0003), and C-reactive protein (P < 0.0001) as well as decreased SHBG (P = 0.001). IRS-2, Glut4, ChREBP, and SREBP exhibited cell-type-specific expression but were not affected by BMI. CD36 and SRBI mRNA were modestly altered in granulosa cells of obese compared with moderate-weight women.
Conclusions: Obese women exhibit an altered ovarian follicular environment, particularly increased metabolite, C-reactive protein, and androgen activity levels, which may be associated with poorer reproductive outcomes typically observed in these patients.
Increasing body mass index is associated with alterations in the ovarian follicular environment. |
| doi_str_mv | 10.1210/jc.2008-2648 |
| format | Article |
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Objective: This study sought to determine whether there are alterations in the preovulatory follicular environment that are likely to influence oocyte developmental competence.
Design, Setting, and Participants: Women attending a private infertility clinic were categorized into body mass index (BMI) groups of moderate (n = 33; BMI 20–24.9 kg/m2), overweight (n = 31; BMI 25–29.9 kg/m2), and obese (n =32; BMI ≥30 kg/m2).
Intervention: For each patient, follicular fluid was recovered from single follicles at oocyte retrieval, granulosa cells were pooled from multiple follicular aspirates and cumulus cells were pooled after separation from the oocytes.
Main Outcome Measures: Follicle fluid was assayed for hormones and metabolites. Granulosa and cumulus cells were analyzed for mRNA expression of insulin signaling components (IRS-2 and Glut4), glucose-regulated genes (ChREBP, ACC, and FAS) and insulin-regulated genes (SREBP-1, CD36, and SR-BI) associated with obesity/insulin resistance.
Results: Increasing BMI was associated with increased follicular fluid insulin (P < 0.001), lactate (P = 0.01), triglycerides (P = 0.0003), and C-reactive protein (P < 0.0001) as well as decreased SHBG (P = 0.001). IRS-2, Glut4, ChREBP, and SREBP exhibited cell-type-specific expression but were not affected by BMI. CD36 and SRBI mRNA were modestly altered in granulosa cells of obese compared with moderate-weight women.
Conclusions: Obese women exhibit an altered ovarian follicular environment, particularly increased metabolite, C-reactive protein, and androgen activity levels, which may be associated with poorer reproductive outcomes typically observed in these patients.
Increasing body mass index is associated with alterations in the ovarian follicular environment.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jc.2008-2648</identifier><identifier>PMID: 19223519</identifier><identifier>CODEN: JCEMAZ</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Adult ; Androgens - metabolism ; Biological and medical sciences ; Body Mass Index ; Body Weight - genetics ; Body Weight - physiology ; C-Reactive Protein - metabolism ; Cumulus Cells - metabolism ; Endocrinopathies ; Feeding. Feeding behavior ; Female ; Follicular Fluid - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression - physiology ; Granulosa Cells - metabolism ; Hormones - biosynthesis ; Hormones - genetics ; Hormones - metabolism ; Humans ; Medical sciences ; Obesity - genetics ; Obesity - metabolism ; Oocytes - physiology ; Ovary - metabolism ; Reproduction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA - biosynthesis ; RNA - isolation & purification ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Vertebrates: endocrinology</subject><ispartof>The journal of clinical endocrinology and metabolism, 2009-05, Vol.94 (5), p.1533-1540</ispartof><rights>Copyright © 2009 by The Endocrine Society</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4083-5f799b6a2c1470d20292045c56c403abfbeab2d58fcc46b6d8888174893ed63</citedby><cites>FETCH-LOGICAL-c4083-5f799b6a2c1470d20292045c56c403abfbeab2d58fcc46b6d8888174893ed63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27913,27914</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21525325$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19223519$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Robker, Rebecca L.</creatorcontrib><creatorcontrib>Akison, Lisa K.</creatorcontrib><creatorcontrib>Bennett, Brenton D.</creatorcontrib><creatorcontrib>Thrupp, Penny N.</creatorcontrib><creatorcontrib>Chura, Lindsay R.</creatorcontrib><creatorcontrib>Russell, Darryl L.</creatorcontrib><creatorcontrib>Lane, Michelle</creatorcontrib><creatorcontrib>Norman, Robert J.</creatorcontrib><title>Obese Women Exhibit Differences in Ovarian Metabolites, Hormones, and Gene Expression Compared with Moderate-Weight Women</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>Context: Obese women experience longer times to conception, even if they are young and cycling regularly, which is suggestive of alterations in ovarian function during the periconceptual period.
Objective: This study sought to determine whether there are alterations in the preovulatory follicular environment that are likely to influence oocyte developmental competence.
Design, Setting, and Participants: Women attending a private infertility clinic were categorized into body mass index (BMI) groups of moderate (n = 33; BMI 20–24.9 kg/m2), overweight (n = 31; BMI 25–29.9 kg/m2), and obese (n =32; BMI ≥30 kg/m2).
Intervention: For each patient, follicular fluid was recovered from single follicles at oocyte retrieval, granulosa cells were pooled from multiple follicular aspirates and cumulus cells were pooled after separation from the oocytes.
Main Outcome Measures: Follicle fluid was assayed for hormones and metabolites. Granulosa and cumulus cells were analyzed for mRNA expression of insulin signaling components (IRS-2 and Glut4), glucose-regulated genes (ChREBP, ACC, and FAS) and insulin-regulated genes (SREBP-1, CD36, and SR-BI) associated with obesity/insulin resistance.
Results: Increasing BMI was associated with increased follicular fluid insulin (P < 0.001), lactate (P = 0.01), triglycerides (P = 0.0003), and C-reactive protein (P < 0.0001) as well as decreased SHBG (P = 0.001). IRS-2, Glut4, ChREBP, and SREBP exhibited cell-type-specific expression but were not affected by BMI. CD36 and SRBI mRNA were modestly altered in granulosa cells of obese compared with moderate-weight women.
Conclusions: Obese women exhibit an altered ovarian follicular environment, particularly increased metabolite, C-reactive protein, and androgen activity levels, which may be associated with poorer reproductive outcomes typically observed in these patients.
Increasing body mass index is associated with alterations in the ovarian follicular environment.</description><subject>Adult</subject><subject>Androgens - metabolism</subject><subject>Biological and medical sciences</subject><subject>Body Mass Index</subject><subject>Body Weight - genetics</subject><subject>Body Weight - physiology</subject><subject>C-Reactive Protein - metabolism</subject><subject>Cumulus Cells - metabolism</subject><subject>Endocrinopathies</subject><subject>Feeding. Feeding behavior</subject><subject>Female</subject><subject>Follicular Fluid - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - physiology</subject><subject>Granulosa Cells - metabolism</subject><subject>Hormones - biosynthesis</subject><subject>Hormones - genetics</subject><subject>Hormones - metabolism</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Obesity - genetics</subject><subject>Obesity - metabolism</subject><subject>Oocytes - physiology</subject><subject>Ovary - metabolism</subject><subject>Reproduction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA - biosynthesis</subject><subject>RNA - isolation & purification</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Vertebrates: endocrinology</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUGP1CAUgInRuLOjN8-Gi562K1Boy9HMrrsmu5mDJuuNAH21jC2M0Druv1_GTvTiC-RB-N6DfCD0hpJLyij5sLOXjJCmYBVvnqEVlVwUNZX1c7QihNFC1uzbGTpPaUcI5VyUL9EZlYyVgsoVetwaSIAfwggeX__unXETvnJdBxG8hYSdx9tfOjrt8T1M2oTBTZAu8G2IY_DHlfYtvgEPuXwfISUXPN6Eca8jtPjgph7fhxainqB4APe9n5bbXqEXnR4SvD7lNfry6frr5ra429583ny8KywnTVmIrpbSVJpZymvSMsIkI1xYUeXzUpvOgDasFU1nLa9M1TY5aM0bWUJblWv0fum6j-HnDGlSo0sWhkF7CHNSVc0ol5xm8GIBbQwpRejUPrpRx0dFiTqKVjurjqLVUXTG3576zmaE9h98MpuBdydAJ6uHLmpvXfrLMSqYKPNYI75whzBMENOPYT5AVD3oYeoVycGrOv8uIZKIvCvypGUuK5cy8G2w0Xn4417twhx91vn_Vz8BsC2mzg</recordid><startdate>200905</startdate><enddate>200905</enddate><creator>Robker, Rebecca L.</creator><creator>Akison, Lisa K.</creator><creator>Bennett, Brenton D.</creator><creator>Thrupp, Penny N.</creator><creator>Chura, Lindsay R.</creator><creator>Russell, Darryl L.</creator><creator>Lane, Michelle</creator><creator>Norman, Robert J.</creator><general>Endocrine Society</general><general>Copyright by The Endocrine Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200905</creationdate><title>Obese Women Exhibit Differences in Ovarian Metabolites, Hormones, and Gene Expression Compared with Moderate-Weight Women</title><author>Robker, Rebecca L. ; Akison, Lisa K. ; Bennett, Brenton D. ; Thrupp, Penny N. ; Chura, Lindsay R. ; Russell, Darryl L. ; Lane, Michelle ; Norman, Robert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4083-5f799b6a2c1470d20292045c56c403abfbeab2d58fcc46b6d8888174893ed63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult</topic><topic>Androgens - metabolism</topic><topic>Biological and medical sciences</topic><topic>Body Mass Index</topic><topic>Body Weight - genetics</topic><topic>Body Weight - physiology</topic><topic>C-Reactive Protein - metabolism</topic><topic>Cumulus Cells - metabolism</topic><topic>Endocrinopathies</topic><topic>Feeding. Feeding behavior</topic><topic>Female</topic><topic>Follicular Fluid - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - physiology</topic><topic>Granulosa Cells - metabolism</topic><topic>Hormones - biosynthesis</topic><topic>Hormones - genetics</topic><topic>Hormones - metabolism</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Obesity - genetics</topic><topic>Obesity - metabolism</topic><topic>Oocytes - physiology</topic><topic>Ovary - metabolism</topic><topic>Reproduction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - biosynthesis</topic><topic>RNA - isolation & purification</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robker, Rebecca L.</creatorcontrib><creatorcontrib>Akison, Lisa K.</creatorcontrib><creatorcontrib>Bennett, Brenton D.</creatorcontrib><creatorcontrib>Thrupp, Penny N.</creatorcontrib><creatorcontrib>Chura, Lindsay R.</creatorcontrib><creatorcontrib>Russell, Darryl L.</creatorcontrib><creatorcontrib>Lane, Michelle</creatorcontrib><creatorcontrib>Norman, Robert J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robker, Rebecca L.</au><au>Akison, Lisa K.</au><au>Bennett, Brenton D.</au><au>Thrupp, Penny N.</au><au>Chura, Lindsay R.</au><au>Russell, Darryl L.</au><au>Lane, Michelle</au><au>Norman, Robert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Obese Women Exhibit Differences in Ovarian Metabolites, Hormones, and Gene Expression Compared with Moderate-Weight Women</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>2009-05</date><risdate>2009</risdate><volume>94</volume><issue>5</issue><spage>1533</spage><epage>1540</epage><pages>1533-1540</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><coden>JCEMAZ</coden><abstract>Context: Obese women experience longer times to conception, even if they are young and cycling regularly, which is suggestive of alterations in ovarian function during the periconceptual period.
Objective: This study sought to determine whether there are alterations in the preovulatory follicular environment that are likely to influence oocyte developmental competence.
Design, Setting, and Participants: Women attending a private infertility clinic were categorized into body mass index (BMI) groups of moderate (n = 33; BMI 20–24.9 kg/m2), overweight (n = 31; BMI 25–29.9 kg/m2), and obese (n =32; BMI ≥30 kg/m2).
Intervention: For each patient, follicular fluid was recovered from single follicles at oocyte retrieval, granulosa cells were pooled from multiple follicular aspirates and cumulus cells were pooled after separation from the oocytes.
Main Outcome Measures: Follicle fluid was assayed for hormones and metabolites. Granulosa and cumulus cells were analyzed for mRNA expression of insulin signaling components (IRS-2 and Glut4), glucose-regulated genes (ChREBP, ACC, and FAS) and insulin-regulated genes (SREBP-1, CD36, and SR-BI) associated with obesity/insulin resistance.
Results: Increasing BMI was associated with increased follicular fluid insulin (P < 0.001), lactate (P = 0.01), triglycerides (P = 0.0003), and C-reactive protein (P < 0.0001) as well as decreased SHBG (P = 0.001). IRS-2, Glut4, ChREBP, and SREBP exhibited cell-type-specific expression but were not affected by BMI. CD36 and SRBI mRNA were modestly altered in granulosa cells of obese compared with moderate-weight women.
Conclusions: Obese women exhibit an altered ovarian follicular environment, particularly increased metabolite, C-reactive protein, and androgen activity levels, which may be associated with poorer reproductive outcomes typically observed in these patients.
Increasing body mass index is associated with alterations in the ovarian follicular environment.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>19223519</pmid><doi>10.1210/jc.2008-2648</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Adult Androgens - metabolism Biological and medical sciences Body Mass Index Body Weight - genetics Body Weight - physiology C-Reactive Protein - metabolism Cumulus Cells - metabolism Endocrinopathies Feeding. Feeding behavior Female Follicular Fluid - metabolism Fundamental and applied biological sciences. Psychology Gene Expression - physiology Granulosa Cells - metabolism Hormones - biosynthesis Hormones - genetics Hormones - metabolism Humans Medical sciences Obesity - genetics Obesity - metabolism Oocytes - physiology Ovary - metabolism Reproduction Reverse Transcriptase Polymerase Chain Reaction RNA - biosynthesis RNA - isolation & purification Vertebrates: anatomy and physiology, studies on body, several organs or systems Vertebrates: endocrinology |
| title | Obese Women Exhibit Differences in Ovarian Metabolites, Hormones, and Gene Expression Compared with Moderate-Weight Women |
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