The distribution of Entamoeba histolytica and Entamoeba dispar in northern, central, and southern Iran
The present study was carried out from August 1999 through February 2002 in order to determine the prevalence of Entamoeba histolytica and Entamoeba dispar in three different climatic regions of Iran by using a PCR-RFLP method. A total of 16,592 stool samples were randomly collected from different a...
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| Veröffentlicht in: | Parasitology research (1987) 2004-09, Vol.94 (2), p.96-100 |
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| container_title | Parasitology research (1987) |
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| creator | HOOSHYAR, H REZAIAN, M KAZEMI, B JEDDI-TEHRANI, M SOLAYMANI-MOHAMMADI, S |
| description | The present study was carried out from August 1999 through February 2002 in order to determine the prevalence of Entamoeba histolytica and Entamoeba dispar in three different climatic regions of Iran by using a PCR-RFLP method. A total of 16,592 stool samples were randomly collected from different age-groups in central, northern, and southern Iran in both urban and rural areas. The samples were examined by direct and formalin-ether concentration methods. A total of 226 samples were positive for E. histolytica/E. dispar cysts. Of these, 101 isolates were cultured and maintained successfully in Robinson's medium and were identified by the PCR-RFLP method. The study showed that 92.1% of isolates were E. dispar and 7.9% were E. histolytica or mixed infections. The ratio of E. histolytica to E. dispar was higher in southern regions (tropical and subtropical) than in the other two regions. This study demonstrated that E. dispar is the predominant species found among "cyst passers" in Iran. |
| doi_str_mv | 10.1007/s00436-004-1175-8 |
| format | Article |
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A total of 16,592 stool samples were randomly collected from different age-groups in central, northern, and southern Iran in both urban and rural areas. The samples were examined by direct and formalin-ether concentration methods. A total of 226 samples were positive for E. histolytica/E. dispar cysts. Of these, 101 isolates were cultured and maintained successfully in Robinson's medium and were identified by the PCR-RFLP method. The study showed that 92.1% of isolates were E. dispar and 7.9% were E. histolytica or mixed infections. The ratio of E. histolytica to E. dispar was higher in southern regions (tropical and subtropical) than in the other two regions. 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A total of 16,592 stool samples were randomly collected from different age-groups in central, northern, and southern Iran in both urban and rural areas. The samples were examined by direct and formalin-ether concentration methods. A total of 226 samples were positive for E. histolytica/E. dispar cysts. Of these, 101 isolates were cultured and maintained successfully in Robinson's medium and were identified by the PCR-RFLP method. The study showed that 92.1% of isolates were E. dispar and 7.9% were E. histolytica or mixed infections. The ratio of E. histolytica to E. dispar was higher in southern regions (tropical and subtropical) than in the other two regions. This study demonstrated that E. dispar is the predominant species found among "cyst passers" in Iran.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Climate</subject><subject>DNA, Protozoan - analysis</subject><subject>Entamoeba - classification</subject><subject>Entamoeba - isolation & purification</subject><subject>Entamoeba histolytica - isolation & purification</subject><subject>Entamoebiasis - epidemiology</subject><subject>Entamoebiasis - parasitology</subject><subject>Feces - parasitology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Humans</subject><subject>Intestinal Diseases, Parasitic - epidemiology</subject><subject>Intestinal Diseases, Parasitic - parasitology</subject><subject>Invertebrates</subject><subject>Iran - epidemiology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Prevalence</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkE1LxDAQhoMouq7-AC-Si56sZpqk3Rxl8QsWvKznMMmmbKVN1qQ9-O-NuwUlMBkyzzuQh5ArYPfAWP2QGBO8KnItAGpZLI7IDAQvC1BSHpMZU7lnAPyMnKf0yRjUlRCn5AxkqTgTckaa9dbRTZuG2JpxaIOnoaFPfsA-OIN0myeh-x5aixT95t8kZ3YYaeupD3HYuujvqHV-iNjd7dEUxv0zfYvoL8hJg11yl9M9Jx_PT-vla7F6f3lbPq4Ky8t6KERpsTFlU9WbBozgdoGqcpYpB86AQGlEaZSQXBhVOlsbhZI5jlxlH4vK8jm5PezdxfA1ujTovk3WdR16F8akq7pkSjDIIBxAG0NK0TV6F9se47cGpn_l6oNcnav-lasXOXM9LR9N7zZ_iclmBm4mAJPFrskft2364yqQVT78B2B1grg</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>HOOSHYAR, H</creator><creator>REZAIAN, M</creator><creator>KAZEMI, B</creator><creator>JEDDI-TEHRANI, M</creator><creator>SOLAYMANI-MOHAMMADI, S</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>The distribution of Entamoeba histolytica and Entamoeba dispar in northern, central, and southern Iran</title><author>HOOSHYAR, H ; REZAIAN, M ; KAZEMI, B ; JEDDI-TEHRANI, M ; SOLAYMANI-MOHAMMADI, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c327t-42cafb2f67df1b43c8a96ec09e1eb14a5b42b94534b92ec7b9a50e3a3910086c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Climate</topic><topic>DNA, Protozoan - analysis</topic><topic>Entamoeba - classification</topic><topic>Entamoeba - isolation & purification</topic><topic>Entamoeba histolytica - isolation & purification</topic><topic>Entamoebiasis - epidemiology</topic><topic>Entamoebiasis - parasitology</topic><topic>Feces - parasitology</topic><topic>Fundamental and applied biological sciences. 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| ispartof | Parasitology research (1987), 2004-09, Vol.94 (2), p.96-100 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Biological and medical sciences Climate DNA, Protozoan - analysis Entamoeba - classification Entamoeba - isolation & purification Entamoeba histolytica - isolation & purification Entamoebiasis - epidemiology Entamoebiasis - parasitology Feces - parasitology Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Humans Intestinal Diseases, Parasitic - epidemiology Intestinal Diseases, Parasitic - parasitology Invertebrates Iran - epidemiology Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length Prevalence |
| title | The distribution of Entamoeba histolytica and Entamoeba dispar in northern, central, and southern Iran |
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