Cytokine production and lymphocyte proliferation in patients with Nocardia brasiliensis actinomycetoma
IFN-gamma, TNF-alpha, IL-4, IL-10 and IL-12 concentrations in the supernatant of peripheral blood mononuclear cell (PBMC) cultures and the in vitro proliferation of PBMC were studied in 25 patients with actinomycetoma caused by Nocardia brasiliensis and in 10 healthy controls from endemic zones. Cel...
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| Veröffentlicht in: | Mycopathologia (1975) 2004-11, Vol.158 (4), p.407-414 |
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| creator | Méndez-Tovar, Luis J Mondragón-González, Rafael Vega-López, Francisco Dockrell, Hazel M Hay, Roderick López-Martínez, Rubén Manzano-Gayosso, Patricia Hernández-Hernández, Francisca Padilla-Desgarennes, Carmen Bonifaz, Alexandro |
| description | IFN-gamma, TNF-alpha, IL-4, IL-10 and IL-12 concentrations in the supernatant of peripheral blood mononuclear cell (PBMC) cultures and the in vitro proliferation of PBMC were studied in 25 patients with actinomycetoma caused by Nocardia brasiliensis and in 10 healthy controls from endemic zones. Cell cultures were stimulated by a N. brasiliensis crude cytoplasmic antigen (NB) and five semi-purified protein fractions (NB2, NB4, NB6, NB8, and NB10) separated by isoelectric. Phytohemagglutinin (PHA) and purified protein derivative (PPD) of Mycobacterium tuberculosis were used as control antigens. Skin tests were performed by injecting 0.1 ml of candidin and PPD intradermally (ID). Patients showed a poor response to tuberculin, while their response to candidin was more than two fold greater than that observed in the controls. Cell proliferation showed no statistically significant differences in either group. IFN-gamma production was higher in the healthy controls than in the patients, whereas TNF-alpha secretion was slightly higher in the patients' cultures. IL-4 was detected in the patients' cultures but not in the controls. IL-10 and IL-12 were present at low concentrations in both groups. These results suggest that patients with actinomycetoma show normal antigen recognition, but with low IFN-gamma production, and higher concentrations of IL-4, IL-10 and TNF-alpha in the patients' PBMC cultures, indicating that they probably have a Th2 type of immune response. |
| doi_str_mv | 10.1007/s11046-004-3126-4 |
| format | Article |
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Cell cultures were stimulated by a N. brasiliensis crude cytoplasmic antigen (NB) and five semi-purified protein fractions (NB2, NB4, NB6, NB8, and NB10) separated by isoelectric. Phytohemagglutinin (PHA) and purified protein derivative (PPD) of Mycobacterium tuberculosis were used as control antigens. Skin tests were performed by injecting 0.1 ml of candidin and PPD intradermally (ID). Patients showed a poor response to tuberculin, while their response to candidin was more than two fold greater than that observed in the controls. Cell proliferation showed no statistically significant differences in either group. IFN-gamma production was higher in the healthy controls than in the patients, whereas TNF-alpha secretion was slightly higher in the patients' cultures. IL-4 was detected in the patients' cultures but not in the controls. IL-10 and IL-12 were present at low concentrations in both groups. These results suggest that patients with actinomycetoma show normal antigen recognition, but with low IFN-gamma production, and higher concentrations of IL-4, IL-10 and TNF-alpha in the patients' PBMC cultures, indicating that they probably have a Th2 type of immune response.</description><identifier>ISSN: 0301-486X</identifier><identifier>EISSN: 1573-0832</identifier><identifier>DOI: 10.1007/s11046-004-3126-4</identifier><identifier>PMID: 15630549</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Adolescent ; Adult ; Aged ; Antibodies ; Antigens ; Cell culture ; Cytokines ; Cytokines - biosynthesis ; Dermatology ; Disease ; Female ; Hospitals ; Humans ; Immunology ; Interferon-gamma - biosynthesis ; Interleukin-10 - biosynthesis ; Interleukin-12 - pharmacology ; Interleukin-2 - biosynthesis ; Interleukin-4 - biosynthesis ; Laboratory animals ; Leukocytes, Mononuclear - metabolism ; Lymphocyte Activation ; Lymphocytes ; Male ; Middle Aged ; Mycobacterium ; Nocardia - isolation & purification ; Nocardia brasiliensis ; Nocardia Infections - immunology ; Nocardia Infections - prevention & control ; Tuberculosis</subject><ispartof>Mycopathologia (1975), 2004-11, Vol.158 (4), p.407-414</ispartof><rights>Kluwer Academic Publishers 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-7a56e7f3ab4869fa1866d97b0e6bad0042c3c401b90c97b5b61cec81f2c3c9613</citedby><cites>FETCH-LOGICAL-c357t-7a56e7f3ab4869fa1866d97b0e6bad0042c3c401b90c97b5b61cec81f2c3c9613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15630549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Méndez-Tovar, Luis J</creatorcontrib><creatorcontrib>Mondragón-González, Rafael</creatorcontrib><creatorcontrib>Vega-López, Francisco</creatorcontrib><creatorcontrib>Dockrell, Hazel M</creatorcontrib><creatorcontrib>Hay, Roderick</creatorcontrib><creatorcontrib>López-Martínez, Rubén</creatorcontrib><creatorcontrib>Manzano-Gayosso, Patricia</creatorcontrib><creatorcontrib>Hernández-Hernández, Francisca</creatorcontrib><creatorcontrib>Padilla-Desgarennes, Carmen</creatorcontrib><creatorcontrib>Bonifaz, Alexandro</creatorcontrib><title>Cytokine production and lymphocyte proliferation in patients with Nocardia brasiliensis actinomycetoma</title><title>Mycopathologia (1975)</title><addtitle>Mycopathologia</addtitle><description>IFN-gamma, TNF-alpha, IL-4, IL-10 and IL-12 concentrations in the supernatant of peripheral blood mononuclear cell (PBMC) cultures and the in vitro proliferation of PBMC were studied in 25 patients with actinomycetoma caused by Nocardia brasiliensis and in 10 healthy controls from endemic zones. Cell cultures were stimulated by a N. brasiliensis crude cytoplasmic antigen (NB) and five semi-purified protein fractions (NB2, NB4, NB6, NB8, and NB10) separated by isoelectric. Phytohemagglutinin (PHA) and purified protein derivative (PPD) of Mycobacterium tuberculosis were used as control antigens. Skin tests were performed by injecting 0.1 ml of candidin and PPD intradermally (ID). Patients showed a poor response to tuberculin, while their response to candidin was more than two fold greater than that observed in the controls. Cell proliferation showed no statistically significant differences in either group. IFN-gamma production was higher in the healthy controls than in the patients, whereas TNF-alpha secretion was slightly higher in the patients' cultures. IL-4 was detected in the patients' cultures but not in the controls. IL-10 and IL-12 were present at low concentrations in both groups. These results suggest that patients with actinomycetoma show normal antigen recognition, but with low IFN-gamma production, and higher concentrations of IL-4, IL-10 and TNF-alpha in the patients' PBMC cultures, indicating that they probably have a Th2 type of immune response.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Cell culture</subject><subject>Cytokines</subject><subject>Cytokines - biosynthesis</subject><subject>Dermatology</subject><subject>Disease</subject><subject>Female</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Immunology</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interleukin-10 - biosynthesis</subject><subject>Interleukin-12 - pharmacology</subject><subject>Interleukin-2 - biosynthesis</subject><subject>Interleukin-4 - biosynthesis</subject><subject>Laboratory animals</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Lymphocyte Activation</subject><subject>Lymphocytes</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mycobacterium</subject><subject>Nocardia - isolation & purification</subject><subject>Nocardia brasiliensis</subject><subject>Nocardia Infections - immunology</subject><subject>Nocardia Infections - prevention & control</subject><subject>Tuberculosis</subject><issn>0301-486X</issn><issn>1573-0832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU9LxDAQxYMo7rr6AbxI8eCtOtO0SXuUxX8gelHwFtI0ZbO2zZq0SL-96e6C4MVTwrzfPObxCDlHuEYAfuMRIWUxQBpTTFicHpA5ZpzGkNPkkMyBAsZpzj5m5MT7NUDYQn5MZpgxCllazEm9HHv7aTodbZytBtUb20Wyq6JmbDcrq8Z-qzSm1k5uRdNFm_DTXe-jb9OvoherpKuMjEonvWmC4o2PZLDqbDsq3dtWnpKjWjZen-3fBXm_v3tbPsbPrw9Py9vnWNGM9zGXGdO8prIMVxe1xJyxquAlaFbKKuRMFFUpYFmACuOsZKi0yrGe5gVDuiBXO99w89egfS9a45VuGtlpO3jBeAJFQum_IPIMIcc0gJd_wLUdXBdCiCTBBKDgLEC4g5Sz3jtdi40zrXSjQBBTVWJXlQgRxFSVmIwv9sZD2erqd2PfDf0B2-SQlA</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Méndez-Tovar, Luis J</creator><creator>Mondragón-González, Rafael</creator><creator>Vega-López, Francisco</creator><creator>Dockrell, Hazel M</creator><creator>Hay, Roderick</creator><creator>López-Martínez, Rubén</creator><creator>Manzano-Gayosso, Patricia</creator><creator>Hernández-Hernández, Francisca</creator><creator>Padilla-Desgarennes, Carmen</creator><creator>Bonifaz, Alexandro</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Cytokine production and lymphocyte proliferation in patients with Nocardia brasiliensis actinomycetoma</title><author>Méndez-Tovar, Luis J ; Mondragón-González, Rafael ; Vega-López, Francisco ; Dockrell, Hazel M ; Hay, Roderick ; López-Martínez, Rubén ; Manzano-Gayosso, Patricia ; Hernández-Hernández, Francisca ; Padilla-Desgarennes, Carmen ; Bonifaz, Alexandro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-7a56e7f3ab4869fa1866d97b0e6bad0042c3c401b90c97b5b61cec81f2c3c9613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Cell culture</topic><topic>Cytokines</topic><topic>Cytokines - 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Academic</collection><jtitle>Mycopathologia (1975)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Méndez-Tovar, Luis J</au><au>Mondragón-González, Rafael</au><au>Vega-López, Francisco</au><au>Dockrell, Hazel M</au><au>Hay, Roderick</au><au>López-Martínez, Rubén</au><au>Manzano-Gayosso, Patricia</au><au>Hernández-Hernández, Francisca</au><au>Padilla-Desgarennes, Carmen</au><au>Bonifaz, Alexandro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytokine production and lymphocyte proliferation in patients with Nocardia brasiliensis actinomycetoma</atitle><jtitle>Mycopathologia (1975)</jtitle><addtitle>Mycopathologia</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>158</volume><issue>4</issue><spage>407</spage><epage>414</epage><pages>407-414</pages><issn>0301-486X</issn><eissn>1573-0832</eissn><abstract>IFN-gamma, TNF-alpha, IL-4, IL-10 and IL-12 concentrations in the supernatant of peripheral blood mononuclear cell (PBMC) cultures and the in vitro proliferation of PBMC were studied in 25 patients with actinomycetoma caused by Nocardia brasiliensis and in 10 healthy controls from endemic zones. Cell cultures were stimulated by a N. brasiliensis crude cytoplasmic antigen (NB) and five semi-purified protein fractions (NB2, NB4, NB6, NB8, and NB10) separated by isoelectric. Phytohemagglutinin (PHA) and purified protein derivative (PPD) of Mycobacterium tuberculosis were used as control antigens. Skin tests were performed by injecting 0.1 ml of candidin and PPD intradermally (ID). Patients showed a poor response to tuberculin, while their response to candidin was more than two fold greater than that observed in the controls. Cell proliferation showed no statistically significant differences in either group. IFN-gamma production was higher in the healthy controls than in the patients, whereas TNF-alpha secretion was slightly higher in the patients' cultures. IL-4 was detected in the patients' cultures but not in the controls. IL-10 and IL-12 were present at low concentrations in both groups. These results suggest that patients with actinomycetoma show normal antigen recognition, but with low IFN-gamma production, and higher concentrations of IL-4, IL-10 and TNF-alpha in the patients' PBMC cultures, indicating that they probably have a Th2 type of immune response.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>15630549</pmid><doi>10.1007/s11046-004-3126-4</doi><tpages>8</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Antibodies Antigens Cell culture Cytokines Cytokines - biosynthesis Dermatology Disease Female Hospitals Humans Immunology Interferon-gamma - biosynthesis Interleukin-10 - biosynthesis Interleukin-12 - pharmacology Interleukin-2 - biosynthesis Interleukin-4 - biosynthesis Laboratory animals Leukocytes, Mononuclear - metabolism Lymphocyte Activation Lymphocytes Male Middle Aged Mycobacterium Nocardia - isolation & purification Nocardia brasiliensis Nocardia Infections - immunology Nocardia Infections - prevention & control Tuberculosis |
| title | Cytokine production and lymphocyte proliferation in patients with Nocardia brasiliensis actinomycetoma |
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