Use of formalin-fixed paraffin-embedded tissue and single-strand conformation polymorphism analysis for polymerase chain reaction of antigen receptor rearrangements in dogs

PCR for antigen receptor gene rearrangement analysis (PARR) is a new diagnostic method for lymphoid neoplasia. In PARR using formalin-fixed paraffin-embedded tissues (PARR-FFPE), control DNA amplification was successful in only three of five samples. The formalin fixation times of the three samples...

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Veröffentlicht in:	Journal of Veterinary Medical Science 2009, Vol.71(4), pp.535-538
Hauptverfasser:	Kaneko, N.(Yamaguchi Univ. (Japan). Faculty of Agriculture), Tanimoto, T, Morimoto, M, Hayashi, T, Shimokawa Miyama, T, Hiraoka, H, Itamoto, K, Une, S, Mizuno, T, Okuda, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals cancer diagnosis canine CHIEN DIAGNOSIS DIAGNOSTIC DIAGNOSTICO Dog Diseases - diagnosis Dog Diseases - genetics Dog Diseases - pathology DOGS Formaldehyde - pharmacology Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor LEUCEMIA LEUCEMIE LEUKAEMIA LINFOMA LYMPHOMA Lymphoma - diagnosis Lymphoma - genetics Lymphoma - pathology Lymphoma - veterinary lymphoma/leukemia LYMPHOME METHODE METHODS METODOS NEOPLASMAS NEOPLASME NEOPLASMS Paraffin Embedding - veterinary PCR PERRO Polymorphism, Single-Stranded Conformational Retrospective Studies Tissue Fixation - methods Tissue Fixation - veterinary tumor diagnosis
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