Phenotypic and Genotypic Characterization of Rhizobia from Diverse Geographical Origin that Nodulate Pachyrhizus species
Legumes from the genus Pachyrhizus, commonly known as yam bean, are cultivated in several countries from the American continent and constitute an alternative source for sustainable starch, oil and protein production. The endosymbionts of these legumes have been poorly studied although it is known th...
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| Veröffentlicht in: | Systematic and applied microbiology 2004-11, Vol.27 (6), p.737-745 |
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| creator | Rodríguez-Navarro, Dulce N. Camacho, María Leidi, Eduardo O. Rivas, Raúl Velázquez, Encarna |
| description | Legumes from the genus
Pachyrhizus, commonly known as yam bean, are cultivated in several countries from the American continent and constitute an alternative source for sustainable starch, oil and protein production. The endosymbionts of these legumes have been poorly studied although it is known that this legume is nodulated by fast and slow growing rhizobia. In this study we have analyzed a collection of strains isolated in several countries using different phenotypic and molecular methods. The results obtained by SDS-PAGE analysis, LPS profiling and TP-RAPD fingerprinting showed the high diversity of the strains analyzed, although all of them presented slow growth in yeast mannitol agar (YMA) medium. These results were confirmed using 16S-23S internal transcribed spacer (ITS) region and complete sequencing of the 16S rRNA gene, showing that most strains analyzed belong to different species of genus
Bradyrhizobium. Three strains were closely related to
B. elkanii and the rest of the strains were related to the phylogenetic group constituted by
B. japonicum,
B. liaoningense,
B. yuanmingense and
B. betae. These results support that the study of rhizobia nodulating unexplored legumes in different geographical locations will allow the discovery of new species able to establish legume symbioses. |
| doi_str_mv | 10.1078/0723202042369839 |
| format | Article |
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Pachyrhizus, commonly known as yam bean, are cultivated in several countries from the American continent and constitute an alternative source for sustainable starch, oil and protein production. The endosymbionts of these legumes have been poorly studied although it is known that this legume is nodulated by fast and slow growing rhizobia. In this study we have analyzed a collection of strains isolated in several countries using different phenotypic and molecular methods. The results obtained by SDS-PAGE analysis, LPS profiling and TP-RAPD fingerprinting showed the high diversity of the strains analyzed, although all of them presented slow growth in yeast mannitol agar (YMA) medium. These results were confirmed using 16S-23S internal transcribed spacer (ITS) region and complete sequencing of the 16S rRNA gene, showing that most strains analyzed belong to different species of genus
Bradyrhizobium. Three strains were closely related to
B. elkanii and the rest of the strains were related to the phylogenetic group constituted by
B. japonicum,
B. liaoningense,
B. yuanmingense and
B. betae. These results support that the study of rhizobia nodulating unexplored legumes in different geographical locations will allow the discovery of new species able to establish legume symbioses.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1078/0723202042369839</identifier><identifier>PMID: 15612632</identifier><identifier>CODEN: SAMIDF</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject><![CDATA[Bacterial Proteins - analysis ; Bacterial Proteins - isolation & purification ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; Bradyrhizobia-biodiversity ; Bradyrhizobium ; Bradyrhizobium - chemistry ; Bradyrhizobium - classification ; Bradyrhizobium - genetics ; Bradyrhizobium - isolation & purification ; DNA Fingerprinting ; DNA, Bacterial - analysis ; DNA, Bacterial - chemistry ; DNA, Bacterial - isolation & purification ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - isolation & purification ; DNA, Ribosomal Spacer - chemistry ; DNA, Ribosomal Spacer - isolation & purification ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Genes, rRNA ; Lipopolysaccharides - analysis ; Lipopolysaccharides - isolation & purification ; LPS profiles ; Microbiology ; Molecular Sequence Data ; Pachyrhizus ; Pachyrhizus - microbiology ; Pachyrhizus ahipa ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Phylogeny ; Proteome - analysis ; Proteome - isolation & purification ; Random Amplified Polymorphic DNA Technique ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - genetics ; SDS-PAGE analysis ; Sequence Analysis, DNA ; TP-RAPD fingerprinting-ITS sequencing]]></subject><ispartof>Systematic and applied microbiology, 2004-11, Vol.27 (6), p.737-745</ispartof><rights>2004 Urban & Fischer Verlag</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Urban & Fischer Verlag Nov 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-ec149e2f523736329f8c5e586980b517846a93387b9cecc9146448874140f8da3</citedby><cites>FETCH-LOGICAL-c434t-ec149e2f523736329f8c5e586980b517846a93387b9cecc9146448874140f8da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/208456048?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16302187$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15612632$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodríguez-Navarro, Dulce N.</creatorcontrib><creatorcontrib>Camacho, María</creatorcontrib><creatorcontrib>Leidi, Eduardo O.</creatorcontrib><creatorcontrib>Rivas, Raúl</creatorcontrib><creatorcontrib>Velázquez, Encarna</creatorcontrib><title>Phenotypic and Genotypic Characterization of Rhizobia from Diverse Geographical Origin that Nodulate Pachyrhizus species</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>Legumes from the genus
Pachyrhizus, commonly known as yam bean, are cultivated in several countries from the American continent and constitute an alternative source for sustainable starch, oil and protein production. The endosymbionts of these legumes have been poorly studied although it is known that this legume is nodulated by fast and slow growing rhizobia. In this study we have analyzed a collection of strains isolated in several countries using different phenotypic and molecular methods. The results obtained by SDS-PAGE analysis, LPS profiling and TP-RAPD fingerprinting showed the high diversity of the strains analyzed, although all of them presented slow growth in yeast mannitol agar (YMA) medium. These results were confirmed using 16S-23S internal transcribed spacer (ITS) region and complete sequencing of the 16S rRNA gene, showing that most strains analyzed belong to different species of genus
Bradyrhizobium. Three strains were closely related to
B. elkanii and the rest of the strains were related to the phylogenetic group constituted by
B. japonicum,
B. liaoningense,
B. yuanmingense and
B. betae. These results support that the study of rhizobia nodulating unexplored legumes in different geographical locations will allow the discovery of new species able to establish legume symbioses.</description><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Bradyrhizobia-biodiversity</subject><subject>Bradyrhizobium</subject><subject>Bradyrhizobium - chemistry</subject><subject>Bradyrhizobium - classification</subject><subject>Bradyrhizobium - genetics</subject><subject>Bradyrhizobium - isolation & purification</subject><subject>DNA Fingerprinting</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - isolation & purification</subject><subject>DNA, Ribosomal Spacer - chemistry</subject><subject>DNA, Ribosomal Spacer - isolation & purification</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, rRNA</subject><subject>Lipopolysaccharides - analysis</subject><subject>Lipopolysaccharides - isolation & purification</subject><subject>LPS profiles</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Pachyrhizus</subject><subject>Pachyrhizus - microbiology</subject><subject>Pachyrhizus ahipa</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Phylogeny</subject><subject>Proteome - analysis</subject><subject>Proteome - isolation & purification</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>SDS-PAGE analysis</subject><subject>Sequence Analysis, DNA</subject><subject>TP-RAPD fingerprinting-ITS sequencing</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkd1rFDEUxYModq2--yRB0LfRfE0-fCurVqHYIvocspk7nZTZyZhkitu_3iy7WCiITyHc37mcew5CLyl5R4nS74linBFGBOPSaG4eoRWVVDfEaPEYrfbjZj8_Qc9yviGECiPpU3RCW0mZ5GyFfl8NMMWym4PHburw-d_fenDJ-QIp3LkS4oRjj78P4S5ugsN9ilv8MdxCylA18Tq5eQjejfgyhesw4TK4gr_FbhldAXzl_LBLVbxknGfwAfJz9KR3Y4YXx_cU_fz86cf6S3Nxef51fXbReMFFacBXz8D6lnHFq2PTa99Cq-u5ZNNSpYV0hnOtNsaD94YKKYTWSlBBet05foreHvbOKf5aIBe7DdnDOLoJ4pKtVIy0hrL_glQpKRXnFXz9ALyJS5rqEZYRLVpJhK4QOUA-xZwT9HZOYevSzlJi993Zh91Vyavj3mWzhe5ecCyrAm-OgMs16j65yYd8z0lOGNWqch8OHNRcbwMkm2vik4cuJPDFdjH828Ufcs6yrw</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Rodríguez-Navarro, Dulce N.</creator><creator>Camacho, María</creator><creator>Leidi, Eduardo O.</creator><creator>Rivas, Raúl</creator><creator>Velázquez, Encarna</creator><general>Elsevier GmbH</general><general>Elsevier</general><general>Elsevier Science Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Phenotypic and Genotypic Characterization of Rhizobia from Diverse Geographical Origin that Nodulate Pachyrhizus species</title><author>Rodríguez-Navarro, Dulce N. ; Camacho, María ; Leidi, Eduardo O. ; Rivas, Raúl ; Velázquez, Encarna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-ec149e2f523736329f8c5e586980b517846a93387b9cecc9146448874140f8da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Bacterial Proteins - analysis</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Bradyrhizobia-biodiversity</topic><topic>Bradyrhizobium</topic><topic>Bradyrhizobium - chemistry</topic><topic>Bradyrhizobium - classification</topic><topic>Bradyrhizobium - genetics</topic><topic>Bradyrhizobium - isolation & purification</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - isolation & purification</topic><topic>DNA, Ribosomal Spacer - chemistry</topic><topic>DNA, Ribosomal Spacer - isolation & purification</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, rRNA</topic><topic>Lipopolysaccharides - analysis</topic><topic>Lipopolysaccharides - isolation & purification</topic><topic>LPS profiles</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Pachyrhizus</topic><topic>Pachyrhizus - microbiology</topic><topic>Pachyrhizus ahipa</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Phylogeny</topic><topic>Proteome - analysis</topic><topic>Proteome - isolation & purification</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>SDS-PAGE analysis</topic><topic>Sequence Analysis, DNA</topic><topic>TP-RAPD fingerprinting-ITS sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodríguez-Navarro, Dulce N.</creatorcontrib><creatorcontrib>Camacho, María</creatorcontrib><creatorcontrib>Leidi, Eduardo O.</creatorcontrib><creatorcontrib>Rivas, Raúl</creatorcontrib><creatorcontrib>Velázquez, Encarna</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodríguez-Navarro, Dulce N.</au><au>Camacho, María</au><au>Leidi, Eduardo O.</au><au>Rivas, Raúl</au><au>Velázquez, Encarna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypic and Genotypic Characterization of Rhizobia from Diverse Geographical Origin that Nodulate Pachyrhizus species</atitle><jtitle>Systematic and applied microbiology</jtitle><addtitle>Syst Appl Microbiol</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>27</volume><issue>6</issue><spage>737</spage><epage>745</epage><pages>737-745</pages><issn>0723-2020</issn><eissn>1618-0984</eissn><coden>SAMIDF</coden><abstract>Legumes from the genus
Pachyrhizus, commonly known as yam bean, are cultivated in several countries from the American continent and constitute an alternative source for sustainable starch, oil and protein production. The endosymbionts of these legumes have been poorly studied although it is known that this legume is nodulated by fast and slow growing rhizobia. In this study we have analyzed a collection of strains isolated in several countries using different phenotypic and molecular methods. The results obtained by SDS-PAGE analysis, LPS profiling and TP-RAPD fingerprinting showed the high diversity of the strains analyzed, although all of them presented slow growth in yeast mannitol agar (YMA) medium. These results were confirmed using 16S-23S internal transcribed spacer (ITS) region and complete sequencing of the 16S rRNA gene, showing that most strains analyzed belong to different species of genus
Bradyrhizobium. Three strains were closely related to
B. elkanii and the rest of the strains were related to the phylogenetic group constituted by
B. japonicum,
B. liaoningense,
B. yuanmingense and
B. betae. These results support that the study of rhizobia nodulating unexplored legumes in different geographical locations will allow the discovery of new species able to establish legume symbioses.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><pmid>15612632</pmid><doi>10.1078/0723202042369839</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0723-2020 |
| ispartof | Systematic and applied microbiology, 2004-11, Vol.27 (6), p.737-745 |
| issn | 0723-2020 1618-0984 |
| language | eng |
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| subjects | Bacterial Proteins - analysis Bacterial Proteins - isolation & purification Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Bradyrhizobia-biodiversity Bradyrhizobium Bradyrhizobium - chemistry Bradyrhizobium - classification Bradyrhizobium - genetics Bradyrhizobium - isolation & purification DNA Fingerprinting DNA, Bacterial - analysis DNA, Bacterial - chemistry DNA, Bacterial - isolation & purification DNA, Ribosomal - chemistry DNA, Ribosomal - isolation & purification DNA, Ribosomal Spacer - chemistry DNA, Ribosomal Spacer - isolation & purification Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Genes, rRNA Lipopolysaccharides - analysis Lipopolysaccharides - isolation & purification LPS profiles Microbiology Molecular Sequence Data Pachyrhizus Pachyrhizus - microbiology Pachyrhizus ahipa Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Phylogeny Proteome - analysis Proteome - isolation & purification Random Amplified Polymorphic DNA Technique RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics SDS-PAGE analysis Sequence Analysis, DNA TP-RAPD fingerprinting-ITS sequencing |
| title | Phenotypic and Genotypic Characterization of Rhizobia from Diverse Geographical Origin that Nodulate Pachyrhizus species |
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