Anti-proliferative and Apoptosis Induction Activity of Green Tea Polyphenols on Human Promyelocytic Leukemia HL-60 Cells
Background: Anti-proliferation and apoptosis inducing effects of green tea polyphenols (GTPs) were studied against human promyeolcytic leukemia HL-60 cells. Materials and Methods: Anti-proliferation activity of GTPs against HL-60 cells and cytotoxicity against normal cells, V79-4 were measured with...
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| Veröffentlicht in: | Anticancer research 2009-04, Vol.29 (4), p.1417-1421 |
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| creator | Han, Dong Hoon Jeong, Jin Hyun Kim, Jeong Hee |
| description | Background: Anti-proliferation and apoptosis inducing effects of green tea polyphenols (GTPs) were studied against human promyeolcytic
leukemia HL-60 cells. Materials and Methods: Anti-proliferation activity of GTPs against HL-60 cells and cytotoxicity against
normal cells, V79-4 were measured with the MTT assay. Nuclear fragmentation of GTP-treated cells was observed with a fluorescence
microscope and flow-cytometric analysis was performed to observe the appearance of apoptotic bodies. Changes in apoptosis-related
protein levels were investigated by Western blot analysis. Results: GTPs significantly reduced the HL-60 proliferation, however,
they did not show significant inhibition in proliferation of normal V79-4 cells. GTP treatment induced apoptosis in HL-60
cells, evidenced by nuclei fragmentation and apoptotic body appearance. The expression level of Bcl-2 was decreased and caspase-3
was activated by GTP treatment. Conclusion: GTPs revealed anti-proliferative and apoptosis-inducing activity against HL-60
cells through the down-regulation of Bcl-2 and activation of caspase-3. |
| format | Article |
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leukemia HL-60 cells. Materials and Methods: Anti-proliferation activity of GTPs against HL-60 cells and cytotoxicity against
normal cells, V79-4 were measured with the MTT assay. Nuclear fragmentation of GTP-treated cells was observed with a fluorescence
microscope and flow-cytometric analysis was performed to observe the appearance of apoptotic bodies. Changes in apoptosis-related
protein levels were investigated by Western blot analysis. Results: GTPs significantly reduced the HL-60 proliferation, however,
they did not show significant inhibition in proliferation of normal V79-4 cells. GTP treatment induced apoptosis in HL-60
cells, evidenced by nuclei fragmentation and apoptotic body appearance. The expression level of Bcl-2 was decreased and caspase-3
was activated by GTP treatment. Conclusion: GTPs revealed anti-proliferative and apoptosis-inducing activity against HL-60
cells through the down-regulation of Bcl-2 and activation of caspase-3.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 19414396</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Apoptosis - drug effects ; Biological and medical sciences ; Caspase 3 - metabolism ; Cell Nucleus - metabolism ; Cell Proliferation - drug effects ; Flavonoids - pharmacology ; Hematologic and hematopoietic diseases ; Humans ; Leukemia, Promyelocytic, Acute - drug therapy ; Leukemia, Promyelocytic, Acute - metabolism ; Leukemia, Promyelocytic, Acute - pathology ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Medical sciences ; Phenols - pharmacology ; Polyphenols ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Tea - chemistry ; Tumor Cells, Cultured ; Tumors</subject><ispartof>Anticancer research, 2009-04, Vol.29 (4), p.1417-1421</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21404374$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19414396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Dong Hoon</creatorcontrib><creatorcontrib>Jeong, Jin Hyun</creatorcontrib><creatorcontrib>Kim, Jeong Hee</creatorcontrib><title>Anti-proliferative and Apoptosis Induction Activity of Green Tea Polyphenols on Human Promyelocytic Leukemia HL-60 Cells</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: Anti-proliferation and apoptosis inducing effects of green tea polyphenols (GTPs) were studied against human promyeolcytic
leukemia HL-60 cells. Materials and Methods: Anti-proliferation activity of GTPs against HL-60 cells and cytotoxicity against
normal cells, V79-4 were measured with the MTT assay. Nuclear fragmentation of GTP-treated cells was observed with a fluorescence
microscope and flow-cytometric analysis was performed to observe the appearance of apoptotic bodies. Changes in apoptosis-related
protein levels were investigated by Western blot analysis. Results: GTPs significantly reduced the HL-60 proliferation, however,
they did not show significant inhibition in proliferation of normal V79-4 cells. GTP treatment induced apoptosis in HL-60
cells, evidenced by nuclei fragmentation and apoptotic body appearance. The expression level of Bcl-2 was decreased and caspase-3
was activated by GTP treatment. Conclusion: GTPs revealed anti-proliferative and apoptosis-inducing activity against HL-60
cells through the down-regulation of Bcl-2 and activation of caspase-3.</description><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Caspase 3 - metabolism</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Proliferation - drug effects</subject><subject>Flavonoids - pharmacology</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Leukemia, Promyelocytic, Acute - drug therapy</subject><subject>Leukemia, Promyelocytic, Acute - metabolism</subject><subject>Leukemia, Promyelocytic, Acute - pathology</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Medical sciences</subject><subject>Phenols - pharmacology</subject><subject>Polyphenols</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Tea - chemistry</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0M1KxDAUBeAiio4_ryDZ6K6QNGnTLIdBnYEBXei63Da3TjRNatKqfXsLjrp0dTYfh3vuQbJgUrFU5pweJgua5TSVlOYnyWmML5QWhSr5cXLClGCCq2KRfC7dYNI-eGtaDDCYdyTgNFn2vh98NJFsnB6bwXhHlnO8m2EiviV3AdGRRwTy4O3U79B5G8mM1mMHjjwE301ofTMNpiFbHF-xM0DW27SgZIXWxvPkqAUb8WKfZ8nT7c3jap1u7-82q-U23XFKhzQXnJW14KUChQ1ogZmEWutaclrO0zKFuqwZAMsFy1RJBbaF4FqilrXQBT9Lrr97541vI8ah6kxs5gvAoR9jVUimpMrUvzCjLKeKlTO83MOx7lBXfTAdhKn6eeoMrvYAYgO2DeAaE39dxgQVXIo_tzPPuw8TsIodWDvX8gpCpipRMcEk_wJsYI4b</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Han, Dong Hoon</creator><creator>Jeong, Jin Hyun</creator><creator>Kim, Jeong Hee</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>7U7</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20090401</creationdate><title>Anti-proliferative and Apoptosis Induction Activity of Green Tea Polyphenols on Human Promyelocytic Leukemia HL-60 Cells</title><author>Han, Dong Hoon ; Jeong, Jin Hyun ; Kim, Jeong Hee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h300t-54318b4389a9ecad4e27abddb730870029ed8b1aa154129804ef643d7ed7b4d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Caspase 3 - metabolism</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Proliferation - drug effects</topic><topic>Flavonoids - pharmacology</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Leukemia, Promyelocytic, Acute - drug therapy</topic><topic>Leukemia, Promyelocytic, Acute - metabolism</topic><topic>Leukemia, Promyelocytic, Acute - pathology</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Medical sciences</topic><topic>Phenols - pharmacology</topic><topic>Polyphenols</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Tea - chemistry</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Dong Hoon</creatorcontrib><creatorcontrib>Jeong, Jin Hyun</creatorcontrib><creatorcontrib>Kim, Jeong Hee</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Dong Hoon</au><au>Jeong, Jin Hyun</au><au>Kim, Jeong Hee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-proliferative and Apoptosis Induction Activity of Green Tea Polyphenols on Human Promyelocytic Leukemia HL-60 Cells</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>29</volume><issue>4</issue><spage>1417</spage><epage>1421</epage><pages>1417-1421</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: Anti-proliferation and apoptosis inducing effects of green tea polyphenols (GTPs) were studied against human promyeolcytic
leukemia HL-60 cells. Materials and Methods: Anti-proliferation activity of GTPs against HL-60 cells and cytotoxicity against
normal cells, V79-4 were measured with the MTT assay. Nuclear fragmentation of GTP-treated cells was observed with a fluorescence
microscope and flow-cytometric analysis was performed to observe the appearance of apoptotic bodies. Changes in apoptosis-related
protein levels were investigated by Western blot analysis. Results: GTPs significantly reduced the HL-60 proliferation, however,
they did not show significant inhibition in proliferation of normal V79-4 cells. GTP treatment induced apoptosis in HL-60
cells, evidenced by nuclei fragmentation and apoptotic body appearance. The expression level of Bcl-2 was decreased and caspase-3
was activated by GTP treatment. Conclusion: GTPs revealed anti-proliferative and apoptosis-inducing activity against HL-60
cells through the down-regulation of Bcl-2 and activation of caspase-3.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>19414396</pmid><tpages>5</tpages></addata></record> |
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| subjects | Apoptosis - drug effects Biological and medical sciences Caspase 3 - metabolism Cell Nucleus - metabolism Cell Proliferation - drug effects Flavonoids - pharmacology Hematologic and hematopoietic diseases Humans Leukemia, Promyelocytic, Acute - drug therapy Leukemia, Promyelocytic, Acute - metabolism Leukemia, Promyelocytic, Acute - pathology Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Medical sciences Phenols - pharmacology Polyphenols Proto-Oncogene Proteins c-bcl-2 - metabolism Tea - chemistry Tumor Cells, Cultured Tumors |
| title | Anti-proliferative and Apoptosis Induction Activity of Green Tea Polyphenols on Human Promyelocytic Leukemia HL-60 Cells |
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