Identification of RAPD markers, in situ DNA content and structural chromosomal diversity in some legumes of the mangrove flora of Orissa

Randomly amplified polymorphic DNA (RAPD) markers, karyotypes and 4C DNA content were analyzed in five legume mangroves belonging to the sub-family Papilinoideae (Dalbergia spinosa, Derris heterophylla and D. indica) and Caesalpinioideae (Caesalpinia crista, Cynometra ramiflora) of the family Fabace...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Genetica 2004-11, Vol.122 (3), p.217-226
Hauptverfasser: Jena, S, Sahoo, P, Mohanty, S, Das, A B
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 226
container_issue 3
container_start_page 217
container_title Genetica
container_volume 122
creator Jena, S
Sahoo, P
Mohanty, S
Das, A B
description Randomly amplified polymorphic DNA (RAPD) markers, karyotypes and 4C DNA content were analyzed in five legume mangroves belonging to the sub-family Papilinoideae (Dalbergia spinosa, Derris heterophylla and D. indica) and Caesalpinioideae (Caesalpinia crista, Cynometra ramiflora) of the family Fabaceae to establish the genetic variability and phylogenetic affinities. Somatic chromosome numbers were reported for the first time in D. spinosa (2n = 20), C. ramiflora (2n = 26) and D. heterophylla (2n = 24) with reconfirmation of the somatic chromosome number in D. indica (2n = 22) and C. crista (2n = 24). Significant intergeneric and interspecific variation of 4C DNA content was observed and that varied from 8.970 pg in C. ramiflora to 28.730 pg in D. indica. From the RAPD analysis, the dendogram showed clustering of Caesalpinia crista and Cynomitra ramiflora into one group (81.80). In the second groups Derris indica and Derris heterophylla were more similar (83.10) than Dalbergia spinosa (85.80). Species-specific DNA markers (900 bp) obtained in D. spinosa from OPN15; 700 and 2000 bp in C. ramiflora from OPN4 and 400 and 800 bp in D. heterophylla and 500 bp DNA fragment in C. crista obtained from OPN-11 were found characteristic RAPD markers of these species. C. crista found more closer affinity to C. ramiflora of the sub-family Caesalpinioideae [genetic distance (1-F) = 0.847]. Derris indica showed closer genetic relation with D. heterophylla [genetic distance (1-F) = 0.856] than D. spinosa [genetic distance (1-F) = 0.876] where Derris and Dalbergia belongs to the sub-family Papilionoideae. By employing these markers the present study has helped to resolve the relationship between the taxonomically diverse leguminous mangroves and study their ability to coexist with mangroves that would shed light on the evolution of mangroves from terrestrial species.
doi_str_mv 10.1007/s10709-004-2040-5
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67195072</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17757997</sourcerecordid><originalsourceid>FETCH-LOGICAL-c355t-482a1e5b461c1ef938d21b8f73af84bf9f6b4490fee20de5dd58b049bb6d29e43</originalsourceid><addsrcrecordid>eNqFkU1rFTEUhoMo9rb6A9xIcNFVR09m8jFZXlo_CsWK6DpkJidt6sykJplC_4E_21zvBcGNqxzC875wzkPIKwZvGYB6lxko0A0Ab1rg0IgnZMOE6hrZC_WUbACYbKQCdUSOc74DAK2kfk6OmJCgBecb8uvS4VKCD6MtIS40evp1--WCzjb9wJTPaFhoDmWlF5-3dIxLqTS1i6O5pHUsa7ITHW9TnGOOc51deKixUB7_BOOMdMKbdca8ay63WIuXmxQfkPopJrv7vU4hZ_uCPPN2yvjy8J6Q7x_efzv_1Fxdf7w83141YydEaXjfWoZi4JKNDL3ueteyofeqs77ng9deDpxr8IgtOBTOiX4ArodBulYj707I6b73PsWfK-Zi5pBHnCa7YFyzkYppAar9L8iUEkprVcE3_4B3cU1LXcIo2amub4WsENtDY4o5J_TmPoV65EfDwOxkmr1MU2WanUwjaub1oXgdZnR_Ewd73W8IApuc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>763738256</pqid></control><display><type>article</type><title>Identification of RAPD markers, in situ DNA content and structural chromosomal diversity in some legumes of the mangrove flora of Orissa</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Jena, S ; Sahoo, P ; Mohanty, S ; Das, A B</creator><creatorcontrib>Jena, S ; Sahoo, P ; Mohanty, S ; Das, A B</creatorcontrib><description>Randomly amplified polymorphic DNA (RAPD) markers, karyotypes and 4C DNA content were analyzed in five legume mangroves belonging to the sub-family Papilinoideae (Dalbergia spinosa, Derris heterophylla and D. indica) and Caesalpinioideae (Caesalpinia crista, Cynometra ramiflora) of the family Fabaceae to establish the genetic variability and phylogenetic affinities. Somatic chromosome numbers were reported for the first time in D. spinosa (2n = 20), C. ramiflora (2n = 26) and D. heterophylla (2n = 24) with reconfirmation of the somatic chromosome number in D. indica (2n = 22) and C. crista (2n = 24). Significant intergeneric and interspecific variation of 4C DNA content was observed and that varied from 8.970 pg in C. ramiflora to 28.730 pg in D. indica. From the RAPD analysis, the dendogram showed clustering of Caesalpinia crista and Cynomitra ramiflora into one group (81.80). In the second groups Derris indica and Derris heterophylla were more similar (83.10) than Dalbergia spinosa (85.80). Species-specific DNA markers (900 bp) obtained in D. spinosa from OPN15; 700 and 2000 bp in C. ramiflora from OPN4 and 400 and 800 bp in D. heterophylla and 500 bp DNA fragment in C. crista obtained from OPN-11 were found characteristic RAPD markers of these species. C. crista found more closer affinity to C. ramiflora of the sub-family Caesalpinioideae [genetic distance (1-F) = 0.847]. Derris indica showed closer genetic relation with D. heterophylla [genetic distance (1-F) = 0.856] than D. spinosa [genetic distance (1-F) = 0.876] where Derris and Dalbergia belongs to the sub-family Papilionoideae. By employing these markers the present study has helped to resolve the relationship between the taxonomically diverse leguminous mangroves and study their ability to coexist with mangroves that would shed light on the evolution of mangroves from terrestrial species.</description><identifier>ISSN: 0016-6707</identifier><identifier>EISSN: 1573-6857</identifier><identifier>DOI: 10.1007/s10709-004-2040-5</identifier><identifier>PMID: 15609544</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Caesalpinia - genetics ; Caesalpinia - metabolism ; Caesalpinia crista ; Cell Nucleus - metabolism ; Chromosomes ; Cynometra ; Cytogenetic Analysis ; Dalbergia ; Dalbergia - genetics ; Dalbergia - metabolism ; Deoxyribonucleic acid ; Derris - genetics ; Derris - metabolism ; Derris heterophylla ; Derris indica ; DNA ; DNA - metabolism ; Fabaceae ; Flora ; Genetic Markers ; Genetic Variation ; Genetics ; Karyotypes ; Mangroves ; Papilionoideae ; Phylogeny ; Polymorphism, Genetic ; Random Amplified Polymorphic DNA Technique</subject><ispartof>Genetica, 2004-11, Vol.122 (3), p.217-226</ispartof><rights>Kluwer Academic Publishers 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-482a1e5b461c1ef938d21b8f73af84bf9f6b4490fee20de5dd58b049bb6d29e43</citedby><cites>FETCH-LOGICAL-c355t-482a1e5b461c1ef938d21b8f73af84bf9f6b4490fee20de5dd58b049bb6d29e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15609544$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jena, S</creatorcontrib><creatorcontrib>Sahoo, P</creatorcontrib><creatorcontrib>Mohanty, S</creatorcontrib><creatorcontrib>Das, A B</creatorcontrib><title>Identification of RAPD markers, in situ DNA content and structural chromosomal diversity in some legumes of the mangrove flora of Orissa</title><title>Genetica</title><addtitle>Genetica</addtitle><description>Randomly amplified polymorphic DNA (RAPD) markers, karyotypes and 4C DNA content were analyzed in five legume mangroves belonging to the sub-family Papilinoideae (Dalbergia spinosa, Derris heterophylla and D. indica) and Caesalpinioideae (Caesalpinia crista, Cynometra ramiflora) of the family Fabaceae to establish the genetic variability and phylogenetic affinities. Somatic chromosome numbers were reported for the first time in D. spinosa (2n = 20), C. ramiflora (2n = 26) and D. heterophylla (2n = 24) with reconfirmation of the somatic chromosome number in D. indica (2n = 22) and C. crista (2n = 24). Significant intergeneric and interspecific variation of 4C DNA content was observed and that varied from 8.970 pg in C. ramiflora to 28.730 pg in D. indica. From the RAPD analysis, the dendogram showed clustering of Caesalpinia crista and Cynomitra ramiflora into one group (81.80). In the second groups Derris indica and Derris heterophylla were more similar (83.10) than Dalbergia spinosa (85.80). Species-specific DNA markers (900 bp) obtained in D. spinosa from OPN15; 700 and 2000 bp in C. ramiflora from OPN4 and 400 and 800 bp in D. heterophylla and 500 bp DNA fragment in C. crista obtained from OPN-11 were found characteristic RAPD markers of these species. C. crista found more closer affinity to C. ramiflora of the sub-family Caesalpinioideae [genetic distance (1-F) = 0.847]. Derris indica showed closer genetic relation with D. heterophylla [genetic distance (1-F) = 0.856] than D. spinosa [genetic distance (1-F) = 0.876] where Derris and Dalbergia belongs to the sub-family Papilionoideae. By employing these markers the present study has helped to resolve the relationship between the taxonomically diverse leguminous mangroves and study their ability to coexist with mangroves that would shed light on the evolution of mangroves from terrestrial species.</description><subject>Caesalpinia - genetics</subject><subject>Caesalpinia - metabolism</subject><subject>Caesalpinia crista</subject><subject>Cell Nucleus - metabolism</subject><subject>Chromosomes</subject><subject>Cynometra</subject><subject>Cytogenetic Analysis</subject><subject>Dalbergia</subject><subject>Dalbergia - genetics</subject><subject>Dalbergia - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>Derris - genetics</subject><subject>Derris - metabolism</subject><subject>Derris heterophylla</subject><subject>Derris indica</subject><subject>DNA</subject><subject>DNA - metabolism</subject><subject>Fabaceae</subject><subject>Flora</subject><subject>Genetic Markers</subject><subject>Genetic Variation</subject><subject>Genetics</subject><subject>Karyotypes</subject><subject>Mangroves</subject><subject>Papilionoideae</subject><subject>Phylogeny</subject><subject>Polymorphism, Genetic</subject><subject>Random Amplified Polymorphic DNA Technique</subject><issn>0016-6707</issn><issn>1573-6857</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1rFTEUhoMo9rb6A9xIcNFVR09m8jFZXlo_CsWK6DpkJidt6sykJplC_4E_21zvBcGNqxzC875wzkPIKwZvGYB6lxko0A0Ab1rg0IgnZMOE6hrZC_WUbACYbKQCdUSOc74DAK2kfk6OmJCgBecb8uvS4VKCD6MtIS40evp1--WCzjb9wJTPaFhoDmWlF5-3dIxLqTS1i6O5pHUsa7ITHW9TnGOOc51deKixUB7_BOOMdMKbdca8ay63WIuXmxQfkPopJrv7vU4hZ_uCPPN2yvjy8J6Q7x_efzv_1Fxdf7w83141YydEaXjfWoZi4JKNDL3ueteyofeqs77ng9deDpxr8IgtOBTOiX4ArodBulYj707I6b73PsWfK-Zi5pBHnCa7YFyzkYppAar9L8iUEkprVcE3_4B3cU1LXcIo2amub4WsENtDY4o5J_TmPoV65EfDwOxkmr1MU2WanUwjaub1oXgdZnR_Ewd73W8IApuc</recordid><startdate>200411</startdate><enddate>200411</enddate><creator>Jena, S</creator><creator>Sahoo, P</creator><creator>Mohanty, S</creator><creator>Das, A B</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200411</creationdate><title>Identification of RAPD markers, in situ DNA content and structural chromosomal diversity in some legumes of the mangrove flora of Orissa</title><author>Jena, S ; Sahoo, P ; Mohanty, S ; Das, A B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-482a1e5b461c1ef938d21b8f73af84bf9f6b4490fee20de5dd58b049bb6d29e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Caesalpinia - genetics</topic><topic>Caesalpinia - metabolism</topic><topic>Caesalpinia crista</topic><topic>Cell Nucleus - metabolism</topic><topic>Chromosomes</topic><topic>Cynometra</topic><topic>Cytogenetic Analysis</topic><topic>Dalbergia</topic><topic>Dalbergia - genetics</topic><topic>Dalbergia - metabolism</topic><topic>Deoxyribonucleic acid</topic><topic>Derris - genetics</topic><topic>Derris - metabolism</topic><topic>Derris heterophylla</topic><topic>Derris indica</topic><topic>DNA</topic><topic>DNA - metabolism</topic><topic>Fabaceae</topic><topic>Flora</topic><topic>Genetic Markers</topic><topic>Genetic Variation</topic><topic>Genetics</topic><topic>Karyotypes</topic><topic>Mangroves</topic><topic>Papilionoideae</topic><topic>Phylogeny</topic><topic>Polymorphism, Genetic</topic><topic>Random Amplified Polymorphic DNA Technique</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jena, S</creatorcontrib><creatorcontrib>Sahoo, P</creatorcontrib><creatorcontrib>Mohanty, S</creatorcontrib><creatorcontrib>Das, A B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genetica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jena, S</au><au>Sahoo, P</au><au>Mohanty, S</au><au>Das, A B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of RAPD markers, in situ DNA content and structural chromosomal diversity in some legumes of the mangrove flora of Orissa</atitle><jtitle>Genetica</jtitle><addtitle>Genetica</addtitle><date>2004-11</date><risdate>2004</risdate><volume>122</volume><issue>3</issue><spage>217</spage><epage>226</epage><pages>217-226</pages><issn>0016-6707</issn><eissn>1573-6857</eissn><abstract>Randomly amplified polymorphic DNA (RAPD) markers, karyotypes and 4C DNA content were analyzed in five legume mangroves belonging to the sub-family Papilinoideae (Dalbergia spinosa, Derris heterophylla and D. indica) and Caesalpinioideae (Caesalpinia crista, Cynometra ramiflora) of the family Fabaceae to establish the genetic variability and phylogenetic affinities. Somatic chromosome numbers were reported for the first time in D. spinosa (2n = 20), C. ramiflora (2n = 26) and D. heterophylla (2n = 24) with reconfirmation of the somatic chromosome number in D. indica (2n = 22) and C. crista (2n = 24). Significant intergeneric and interspecific variation of 4C DNA content was observed and that varied from 8.970 pg in C. ramiflora to 28.730 pg in D. indica. From the RAPD analysis, the dendogram showed clustering of Caesalpinia crista and Cynomitra ramiflora into one group (81.80). In the second groups Derris indica and Derris heterophylla were more similar (83.10) than Dalbergia spinosa (85.80). Species-specific DNA markers (900 bp) obtained in D. spinosa from OPN15; 700 and 2000 bp in C. ramiflora from OPN4 and 400 and 800 bp in D. heterophylla and 500 bp DNA fragment in C. crista obtained from OPN-11 were found characteristic RAPD markers of these species. C. crista found more closer affinity to C. ramiflora of the sub-family Caesalpinioideae [genetic distance (1-F) = 0.847]. Derris indica showed closer genetic relation with D. heterophylla [genetic distance (1-F) = 0.856] than D. spinosa [genetic distance (1-F) = 0.876] where Derris and Dalbergia belongs to the sub-family Papilionoideae. By employing these markers the present study has helped to resolve the relationship between the taxonomically diverse leguminous mangroves and study their ability to coexist with mangroves that would shed light on the evolution of mangroves from terrestrial species.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>15609544</pmid><doi>10.1007/s10709-004-2040-5</doi><tpages>10</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0016-6707
ispartof Genetica, 2004-11, Vol.122 (3), p.217-226
issn 0016-6707
1573-6857
language eng
recordid cdi_proquest_miscellaneous_67195072
source MEDLINE; SpringerNature Journals
subjects Caesalpinia - genetics
Caesalpinia - metabolism
Caesalpinia crista
Cell Nucleus - metabolism
Chromosomes
Cynometra
Cytogenetic Analysis
Dalbergia
Dalbergia - genetics
Dalbergia - metabolism
Deoxyribonucleic acid
Derris - genetics
Derris - metabolism
Derris heterophylla
Derris indica
DNA
DNA - metabolism
Fabaceae
Flora
Genetic Markers
Genetic Variation
Genetics
Karyotypes
Mangroves
Papilionoideae
Phylogeny
Polymorphism, Genetic
Random Amplified Polymorphic DNA Technique
title Identification of RAPD markers, in situ DNA content and structural chromosomal diversity in some legumes of the mangrove flora of Orissa
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T10%3A53%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20RAPD%20markers,%20in%20situ%20DNA%20content%20and%20structural%20chromosomal%20diversity%20in%20some%20legumes%20of%20the%20mangrove%20flora%20of%20Orissa&rft.jtitle=Genetica&rft.au=Jena,%20S&rft.date=2004-11&rft.volume=122&rft.issue=3&rft.spage=217&rft.epage=226&rft.pages=217-226&rft.issn=0016-6707&rft.eissn=1573-6857&rft_id=info:doi/10.1007/s10709-004-2040-5&rft_dat=%3Cproquest_cross%3E17757997%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=763738256&rft_id=info:pmid/15609544&rfr_iscdi=true