Genotypic and technological characterization of enterococci isolated from artisanal Fiore Sardo cheese
A total of 118 enterococcal strains isolated from artisanal Fiore Sardo cheese were characterized technologically and genetically. The presence of potential virulence factors was also investigated. Strains were classified as Ec. faecium (84 strains), Ec. durans (24 strains) and Ec. faecalis (10 stra...
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| Veröffentlicht in: | Journal of dairy research 2004-11, Vol.71 (4), p.444-450 |
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| creator | Cosentino, Sofia Pisano, M Barbara Corda, Arianna Fadda, M Elisabetta Piras, Carla |
| description | A total of 118 enterococcal strains isolated from artisanal Fiore Sardo cheese were characterized technologically and genetically. The presence of potential virulence factors was also investigated. Strains were classified as Ec. faecium (84 strains), Ec. durans (24 strains) and Ec. faecalis (10 strains). RAPD-PCR analysis with two different primers (M13 and XD9) confirmed species identification and proved useful for the detection of interstrain variations, especially among Ec. faecium isolates. Most strains could hydrolyse casein and had weak acidifying activity in milk. None of the isolates produced lipolytic reactions. Gelatinase activity was observed in two strains of Ec. faecalis and one strain of Ec. durans. β-Haemolysis on horses' blood was never detected in any of the strains, independently of species. Most strains produced tyramine from tyrosine but none decarboxylated lysine, histidine or ornithine. Overall, a wide spectrum of resistance was observed. Almost all strains were resistant to the aminoglycosides, gentamycin, kanamycin, streptomycin, neomycin, and to the semisynthetic penicillin, oxacillin, but resistance to vancomycin was not widespread among our strains: only one Ec. faecium and one Ec. durans strain were found to be vancomycin resistant. Our results show a certain diversity in technological traits of the enterococcal strains isolated from artisanal Fiore Sardo, together with a low incidence of some potentially pathogenic traits of health concern. |
| doi_str_mv | 10.1017/S002202990400041X |
| format | Article |
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The presence of potential virulence factors was also investigated. Strains were classified as Ec. faecium (84 strains), Ec. durans (24 strains) and Ec. faecalis (10 strains). RAPD-PCR analysis with two different primers (M13 and XD9) confirmed species identification and proved useful for the detection of interstrain variations, especially among Ec. faecium isolates. Most strains could hydrolyse casein and had weak acidifying activity in milk. None of the isolates produced lipolytic reactions. Gelatinase activity was observed in two strains of Ec. faecalis and one strain of Ec. durans. β-Haemolysis on horses' blood was never detected in any of the strains, independently of species. Most strains produced tyramine from tyrosine but none decarboxylated lysine, histidine or ornithine. Overall, a wide spectrum of resistance was observed. Almost all strains were resistant to the aminoglycosides, gentamycin, kanamycin, streptomycin, neomycin, and to the semisynthetic penicillin, oxacillin, but resistance to vancomycin was not widespread among our strains: only one Ec. faecium and one Ec. durans strain were found to be vancomycin resistant. Our results show a certain diversity in technological traits of the enterococcal strains isolated from artisanal Fiore Sardo, together with a low incidence of some potentially pathogenic traits of health concern.</description><identifier>ISSN: 0022-0299</identifier><identifier>EISSN: 1469-7629</identifier><identifier>DOI: 10.1017/S002202990400041X</identifier><identifier>PMID: 15605711</identifier><identifier>CODEN: JDRSAN</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animal productions ; Biological and medical sciences ; blood ; casein ; Cheese - analysis ; Cheese - microbiology ; cheeses ; Dairy products ; Drug Resistance, Microbial ; enterococci ; Enterococcus ; Enterococcus - drug effects ; Enterococcus - genetics ; Enterococcus - isolation & purification ; Enterococcus durans ; Enterococcus faecalis ; Enterococcus faecalis - genetics ; Enterococcus faecalis - isolation & purification ; Enterococcus faecium ; Enterococcus faecium - genetics ; Enterococcus faecium - isolation & purification ; Fiore Sardo ; Food industries ; Fundamental and applied biological sciences. Psychology ; Genotype ; gentamicin ; Horses ; Italy ; kanamycin ; lysine decarboxylase ; milk ; Milk and cheese industries. Ice creams ; neomycin ; oxacillin ; Phenotype ; Random Amplified Polymorphic DNA Technique ; species identification ; streptomycin ; Terrestrial animal productions ; traditional technology ; tyrosine ; vancomycin ; Vertebrates ; virulence ; Virulence Factors - analysis</subject><ispartof>Journal of dairy research, 2004-11, Vol.71 (4), p.444-450</ispartof><rights>Proprietors of Journal of Dairy Research 2004</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c607t-79b95b0d80486d553c658068bc957d3904737d871f831ec95071af06e13f15663</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S002202990400041X/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,776,780,27901,27902,55603</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16319736$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15605711$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cosentino, Sofia</creatorcontrib><creatorcontrib>Pisano, M Barbara</creatorcontrib><creatorcontrib>Corda, Arianna</creatorcontrib><creatorcontrib>Fadda, M Elisabetta</creatorcontrib><creatorcontrib>Piras, Carla</creatorcontrib><title>Genotypic and technological characterization of enterococci isolated from artisanal Fiore Sardo cheese</title><title>Journal of dairy research</title><addtitle>Journal of Dairy Research</addtitle><description>A total of 118 enterococcal strains isolated from artisanal Fiore Sardo cheese were characterized technologically and genetically. The presence of potential virulence factors was also investigated. Strains were classified as Ec. faecium (84 strains), Ec. durans (24 strains) and Ec. faecalis (10 strains). RAPD-PCR analysis with two different primers (M13 and XD9) confirmed species identification and proved useful for the detection of interstrain variations, especially among Ec. faecium isolates. Most strains could hydrolyse casein and had weak acidifying activity in milk. None of the isolates produced lipolytic reactions. Gelatinase activity was observed in two strains of Ec. faecalis and one strain of Ec. durans. β-Haemolysis on horses' blood was never detected in any of the strains, independently of species. Most strains produced tyramine from tyrosine but none decarboxylated lysine, histidine or ornithine. Overall, a wide spectrum of resistance was observed. Almost all strains were resistant to the aminoglycosides, gentamycin, kanamycin, streptomycin, neomycin, and to the semisynthetic penicillin, oxacillin, but resistance to vancomycin was not widespread among our strains: only one Ec. faecium and one Ec. durans strain were found to be vancomycin resistant. Our results show a certain diversity in technological traits of the enterococcal strains isolated from artisanal Fiore Sardo, together with a low incidence of some potentially pathogenic traits of health concern.</description><subject>Animal productions</subject><subject>Biological and medical sciences</subject><subject>blood</subject><subject>casein</subject><subject>Cheese - analysis</subject><subject>Cheese - microbiology</subject><subject>cheeses</subject><subject>Dairy products</subject><subject>Drug Resistance, Microbial</subject><subject>enterococci</subject><subject>Enterococcus</subject><subject>Enterococcus - drug effects</subject><subject>Enterococcus - genetics</subject><subject>Enterococcus - isolation & purification</subject><subject>Enterococcus durans</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - genetics</subject><subject>Enterococcus faecalis - isolation & purification</subject><subject>Enterococcus faecium</subject><subject>Enterococcus faecium - genetics</subject><subject>Enterococcus faecium - isolation & purification</subject><subject>Fiore Sardo</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>gentamicin</subject><subject>Horses</subject><subject>Italy</subject><subject>kanamycin</subject><subject>lysine decarboxylase</subject><subject>milk</subject><subject>Milk and cheese industries. Ice creams</subject><subject>neomycin</subject><subject>oxacillin</subject><subject>Phenotype</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>species identification</subject><subject>streptomycin</subject><subject>Terrestrial animal productions</subject><subject>traditional technology</subject><subject>tyrosine</subject><subject>vancomycin</subject><subject>Vertebrates</subject><subject>virulence</subject><subject>Virulence Factors - analysis</subject><issn>0022-0299</issn><issn>1469-7629</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkdFqFDEUhoModlt9AG90EOrd6DnJTDK51GJXy4KUteJdyGSSberMZE1mwfr0ZtnBBUW8Csn5zk8-fkKeIbxGQPFmDUApUCmhAoAKvz4gC6y4LAWn8iFZ7Mflfn5CTlO6A0AGkj8mJ1hzqAXigrilHcN0v_Wm0GNXTNbcjqEPG290X5hbHbWZbPQ_9eTDWARX2DHfgwnG-MKn0OvJdoWLYSh0nHzSY9679CHaYq1jF3KGtck-IY-c7pN9Op9n5Oby_eeLD-Xq0_LjxdtVaTiIqRSylXULXQNVw7u6ZobXDfCmNbIWHcuegomuEegahjY_gkDtgFtkLjtxdkZeHXK3MXzf2TSpwSdj-16PNuyS4gIlCvg_iELwikKTwZd_gHdhF7NlUpQKKRkXMkN4gEwMKUXr1Db6Qcd7haD2Vam_qso7z-fgXTvY7rgxd5OB8xnQKbfhoh6NT0eOM5SC7VXKA-fTZH_8nuv4LesyUSu-vFZXX66uqVy_U6vMvzjwTgelNzFn3qwpYP4Y0pxKM8FmHT200Xcbe5T-t9AvLrDBvw</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Cosentino, Sofia</creator><creator>Pisano, M Barbara</creator><creator>Corda, Arianna</creator><creator>Fadda, M Elisabetta</creator><creator>Piras, Carla</creator><general>Cambridge University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Genotypic and technological characterization of enterococci isolated from artisanal Fiore Sardo cheese</title><author>Cosentino, Sofia ; Pisano, M Barbara ; Corda, Arianna ; Fadda, M Elisabetta ; Piras, Carla</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c607t-79b95b0d80486d553c658068bc957d3904737d871f831ec95071af06e13f15663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animal productions</topic><topic>Biological and medical sciences</topic><topic>blood</topic><topic>casein</topic><topic>Cheese - analysis</topic><topic>Cheese - microbiology</topic><topic>cheeses</topic><topic>Dairy products</topic><topic>Drug Resistance, Microbial</topic><topic>enterococci</topic><topic>Enterococcus</topic><topic>Enterococcus - drug effects</topic><topic>Enterococcus - genetics</topic><topic>Enterococcus - isolation & purification</topic><topic>Enterococcus durans</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecalis - genetics</topic><topic>Enterococcus faecalis - isolation & purification</topic><topic>Enterococcus faecium</topic><topic>Enterococcus faecium - genetics</topic><topic>Enterococcus faecium - isolation & purification</topic><topic>Fiore Sardo</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>gentamicin</topic><topic>Horses</topic><topic>Italy</topic><topic>kanamycin</topic><topic>lysine decarboxylase</topic><topic>milk</topic><topic>Milk and cheese industries. Ice creams</topic><topic>neomycin</topic><topic>oxacillin</topic><topic>Phenotype</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>species identification</topic><topic>streptomycin</topic><topic>Terrestrial animal productions</topic><topic>traditional technology</topic><topic>tyrosine</topic><topic>vancomycin</topic><topic>Vertebrates</topic><topic>virulence</topic><topic>Virulence Factors - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cosentino, Sofia</creatorcontrib><creatorcontrib>Pisano, M Barbara</creatorcontrib><creatorcontrib>Corda, Arianna</creatorcontrib><creatorcontrib>Fadda, M Elisabetta</creatorcontrib><creatorcontrib>Piras, Carla</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cosentino, Sofia</au><au>Pisano, M Barbara</au><au>Corda, Arianna</au><au>Fadda, M Elisabetta</au><au>Piras, Carla</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotypic and technological characterization of enterococci isolated from artisanal Fiore Sardo cheese</atitle><jtitle>Journal of dairy research</jtitle><addtitle>Journal of Dairy Research</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>71</volume><issue>4</issue><spage>444</spage><epage>450</epage><pages>444-450</pages><issn>0022-0299</issn><eissn>1469-7629</eissn><coden>JDRSAN</coden><abstract>A total of 118 enterococcal strains isolated from artisanal Fiore Sardo cheese were characterized technologically and genetically. The presence of potential virulence factors was also investigated. Strains were classified as Ec. faecium (84 strains), Ec. durans (24 strains) and Ec. faecalis (10 strains). RAPD-PCR analysis with two different primers (M13 and XD9) confirmed species identification and proved useful for the detection of interstrain variations, especially among Ec. faecium isolates. Most strains could hydrolyse casein and had weak acidifying activity in milk. None of the isolates produced lipolytic reactions. Gelatinase activity was observed in two strains of Ec. faecalis and one strain of Ec. durans. β-Haemolysis on horses' blood was never detected in any of the strains, independently of species. Most strains produced tyramine from tyrosine but none decarboxylated lysine, histidine or ornithine. Overall, a wide spectrum of resistance was observed. Almost all strains were resistant to the aminoglycosides, gentamycin, kanamycin, streptomycin, neomycin, and to the semisynthetic penicillin, oxacillin, but resistance to vancomycin was not widespread among our strains: only one Ec. faecium and one Ec. durans strain were found to be vancomycin resistant. Our results show a certain diversity in technological traits of the enterococcal strains isolated from artisanal Fiore Sardo, together with a low incidence of some potentially pathogenic traits of health concern.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>15605711</pmid><doi>10.1017/S002202990400041X</doi><tpages>7</tpages></addata></record> |
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| subjects | Animal productions Biological and medical sciences blood casein Cheese - analysis Cheese - microbiology cheeses Dairy products Drug Resistance, Microbial enterococci Enterococcus Enterococcus - drug effects Enterococcus - genetics Enterococcus - isolation & purification Enterococcus durans Enterococcus faecalis Enterococcus faecalis - genetics Enterococcus faecalis - isolation & purification Enterococcus faecium Enterococcus faecium - genetics Enterococcus faecium - isolation & purification Fiore Sardo Food industries Fundamental and applied biological sciences. Psychology Genotype gentamicin Horses Italy kanamycin lysine decarboxylase milk Milk and cheese industries. Ice creams neomycin oxacillin Phenotype Random Amplified Polymorphic DNA Technique species identification streptomycin Terrestrial animal productions traditional technology tyrosine vancomycin Vertebrates virulence Virulence Factors - analysis |
| title | Genotypic and technological characterization of enterococci isolated from artisanal Fiore Sardo cheese |
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