Pediococcus lolii sp. nov., isolated from ryegrass silage

1 Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan 2 Asama Chemical Co. Ltd, 20-3 Nihonbashi-Kodenma-cho, Chuo-ku, Tokyo 103-0001, Japan 3 Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan Correspondence Katsumi Doi doi{at}agr.kyushu-u.ac.jp A Gram-positive, coccus-shaped, lactic acid bacterium, strain NGRI 0510Q T , was isolated from ryegrass silage produced in Okinawa Prefecture, Japan. The cell is non-spore-forming, non-motile, and occurs in pairs or tetrads. The strain is homofermentative and produces D - and L -lactic acid from glucose. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NGRI 0510Q T belongs to the genus Pediococcus and clusters within the Pediococcus acidilactici and Pediococcus pentosaceus group, with 98.2 and 96.9 % sequence identity, respectively. DNA–DNA relatedness between strain NGRI 0510Q T and P. acidilactici JCM 8797 T and P. pentosaceus JCM 5890 T was 19.3 and 17.3 %, respectively. Based on its phenotypic characteristics, phylogenetic relationship and DNA–DNA relatedness, NGRI 0510Q T (=JCM 15055 T =DSM 19927 T ) represents the type strain of a novel species, for which the name Pediococcus lolii sp. nov. is proposed. Abbreviations: ERIC-PCR, enterobacterial repetitive intergenic consensus PCR; LAB, lactic acid bacteria, RAPD-PCR, random amplified polymorphic DNA PCR The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain NGRI 0510Q T is AB362985 .