Large Scale Localization of Protein Phosphorylation by Use of Electron Capture Dissociation Mass Spectrometry

We used on-line electron capture dissociation (ECD) for the large scale identification and localization of sites of phosphorylation. Each FT-ICR ECD event was paired with a linear ion trap collision-induced dissociation (CID) event, allowing a direct comparison of the relative merits of ECD and CID...

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Veröffentlicht in:	Molecular & cellular proteomics 2009-05, Vol.8 (5), p.904-912
Hauptverfasser:	Sweet, Steve M M, Bailey, Christopher M, Cunningham, Debbie L, Heath, John K, Cooper, Helen J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Mass Spectrometry - methods Mice Molecular Sequence Data NIH 3T3 Cells Phosphopeptides - analysis Phosphopeptides - chemistry Phosphorylation Proteins - chemistry Proteins - metabolism Reproducibility of Results
Online-Zugang:	Volltext
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