Digital Ion Trap Mass Spectrometer for Probing the Structure of Biological Macromolecules by Gas Phase X-ray Scattering
Small-angle X-ray scattering is a technique for the characterization and structural analysis of a variety of materials including biological macromolecules and polymers. For the conformational analysis of proteins, the interaction between sample and X-rays is generally performed when the proteins are...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2009-05, Vol.81 (9), p.3392-3397 |
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| container_title | Analytical chemistry (Washington) |
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| creator | McCullough, Bryan J Entwistle, Andrew Konishi, Ikuo Buffey, Steven Hasnain, S. Samar Brancia, Francesco L Grossmann, J. Günter Gaskell, Simon J |
| description | Small-angle X-ray scattering is a technique for the characterization and structural analysis of a variety of materials including biological macromolecules and polymers. For the conformational analysis of proteins, the interaction between sample and X-rays is generally performed when the proteins are present in solution. Here a three-dimensional digital ion trap interfaced with a high intensity X-ray source is built to prove that X-ray scattering can be performed on ions isolated in gas-phase. Initial experiments on an unresolved ion population of multiply charged cytochrome C ions indicate that a small-angle X-ray scattering signal can be detected and that partial structural information can be extracted about the overall molecular structure of protein ions. |
| doi_str_mv | 10.1021/ac802662d |
| format | Article |
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| subjects | Analytical chemistry Biopolymers - chemistry Chemistry Cytochromes c - chemistry Equipment Design Exact sciences and technology Gases - chemistry Ions Mass spectrometry Mass Spectrometry - instrumentation Mass Spectrometry - methods Molecular structure Peptides - chemistry Polymers Proteins Scattering, Small Angle Spectrometric and optical methods X-Ray Diffraction X-rays |
| title | Digital Ion Trap Mass Spectrometer for Probing the Structure of Biological Macromolecules by Gas Phase X-ray Scattering |
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