Maize recombinant non-C4 NADP-malic enzyme: A novel dimeric malic enzyme with high specific activity

Among the different isoforms of NADP-malic enzyme (NADP-ME) involved in a wide range of metabolic pathways in plants, the NADP-ME that participates in C4-photosynthesis is the most studied. In the present work, the expression in E. coli of a cDNA encoding for a maize non-photosynthetic NADP-ME is pr...

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Veröffentlicht in:	Plant molecular biology 2004-05, Vol.55 (1), p.97-107
Hauptverfasser:	Saigo, M, Bologna, F.P, Maurino, V.G, Detarsio, E, Andreo, C.S, Drincovich, M.F
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Blotting, Western Cloning, Molecular complementary DNA Corn Dimerization DNA, Complementary - chemistry DNA, Complementary - genetics E coli enzyme activity Escherichia coli gene expression Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant immunohistochemistry Isoenzymes - chemistry Isoenzymes - genetics Isoenzymes - metabolism isozymes Kinetics leaves Malate Dehydrogenase - chemistry Malate Dehydrogenase - genetics Malate Dehydrogenase - metabolism malic enzyme Molecular Sequence Data Molecular Weight nucleotide sequences Photosynthesis protein quaternary structure recombinant proteins Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Reverse Transcriptase Polymerase Chain Reaction Roots Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid substrate specificity Zea mays Zea mays - enzymology Zea mays - genetics
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