Biophysical Characterization of Human XRCC1 and Its Binding to Damaged and Undamaged DNA

The human DNA repair protein, hXRCC1, which is required for DNA single-strand break repair and genetic stability was produced as a histidine-tagged polypeptide in Escherichia coli, purified by affinity chromatography, and subjected to sedimentation and spectroscopic analyses. This study represents t...

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Veröffentlicht in:	Biochemistry (Easton) 2004-12, Vol.43 (51), p.16505-16514
Hauptverfasser:	Mani, Rajam S, Karimi-Busheri, Feridoun, Fanta, Mesfin, Caldecott, Keith W, Cass, Carol E, Weinfeld, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Affinity Circular Dichroism DNA - metabolism DNA Damage - physiology DNA-Binding Proteins - genetics DNA-Binding Proteins - isolation & purification DNA-Binding Proteins - metabolism Escherichia coli Humans Kinetics Ligands Spectrometry, Fluorescence X-ray Repair Cross Complementing Protein 1
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