High-resolution imaging of single fluorescent molecules with the optical near-field of a metal tip

We show that a concentration of light at a metal tip allows near-field optical imaging of single fluorescent dye molecules at very high resolution, despite strong quenching effects. Details as small as 10 nm were observed in the fluorescence patterns of single Cy-3 dyes bound to the termini of DNA....

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Veröffentlicht in:	Physical review letters 2004-11, Vol.93 (20), p.200801.1-200801.4, Article 200801
Hauptverfasser:	FREY, Heinrich G, WITT, Susanne, FELDERER, Karin, GUCKENBERGER, Reinhard
Format:	Artikel
Sprache:	eng
Schlagworte:	Atomic and molecular physics Biological and medical sciences Exact sciences and technology Fluorescence and phosphorescence spectra Fluorescence and phosphorescence radiationless transitions, quenching (intersystem crossing, internal conversion) Fundamental and applied biological sciences. Psychology General aspects, investigation technics, apparatus Instruments, apparatus, components and techniques common to several branches of physics and astronomy Molecular properties and interactions with photons Near-field scanning optical microscopes Near-field scanning optical microscopy Physics Scanning probe microscopes, components and techniques Tissues, organs and organisms biophysics
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